FastQCFastQC Report
Fri 27 May 2016
SRR1233120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45501957
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT3990310.8769534901542806No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC3296410.7244545547788197No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA3236170.7112155637613564No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT2460460.5407371819194502No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT2360700.5188128501813669No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT2301860.5058815382380147No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1863150.4094659049499783No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1663180.3655183446285618No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1659020.3646040982369176No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA1527840.3357745689927139No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1415490.3110833232953035No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG1325390.2912819771685864No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG1321480.2904226734687477No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA1306200.287064576145593No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA1276230.2804780462519447No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT1102840.2423719929232934No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1068350.23479209916180088No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG1033810.22720121686194727No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA1026660.22562985587630877No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT1007650.22145201359141542No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT986630.21683243206440547No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT911680.20036061306110417No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT878300.19302466485122827No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT862840.1896270087899736No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA840170.18464480549704707No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC822900.18084936434712026No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT762250.16752026731509592No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC755730.1660873619128074No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG729280.16027442511978113No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA726020.15955797241863687No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT719110.15803935641713168No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA700890.15403513303834382No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA697950.15338900698271066No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA696000.15296045398662741No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA682080.14990124490689488No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA678780.14917600137506173No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA665330.1462200845559236No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA650800.14302681530818553No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT626890.13777209626390355No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG600150.13189542594838283No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG593380.13040757785428878No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT577900.12700552637768966No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA572570.1258341481883955No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT571330.12556163243704002No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG565940.12437706800171254No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT552940.12152004802782439No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG552010.12131566121430777No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA534270.11741692780378656No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT531870.11688947796245336No Hit
GGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTGGTTGGCCCCCAA531790.11687189630107558No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG517280.11368302246868194No Hit
ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA512320.11259295946326No Hit
ATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTT497400.10931397961630529No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT492830.10830962721009998No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT489270.10752724327878907No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT475140.10442188233793989No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG464860.102162638850896No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG464460.10207473054400715No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA462340.10160881651749616No Hit
GTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGT461040.10132311452010735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT545850.083.997871
GATCGGG52400.066.3639761
GCGTGTT700850.063.952752
GAGCGGC61400.062.4903229
ATCGGGA60150.057.7341422
CGTGTTT829250.053.4451453
TCGAACG37400.049.801912
CGAACGT68950.047.4638861
CGCCGGA68550.044.22848546-47
TCGGGAG70700.042.177323
GTGTTTT1074900.040.5162934
GTAGATA473550.040.3585852
GCCGGAT85400.039.9396446-47
GTCGAAC48000.039.6971781
CCGGATC84350.038.50853348-49
TCGGGGG124950.038.4263138-39
CGCGTTT16250.038.4024351
TAGATAT515250.037.5346263
CGGGAGA82500.036.9477124
GATATAG523700.036.4423875
Produced by FastQC (version 0.11.5)