FastQCFastQC Report
Fri 27 May 2016
SRR1233120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45501957
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2888160.6347331390603705No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT2677090.588346123222788No Hit
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG2545650.5594594535791064No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG2153770.4733356853200842No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA2090620.45945716137000436No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA2068960.45469692655197225No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA2062070.45318270596581156No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT2060750.4528926085530783No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1565770.34411047419345064No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1552190.3411259871745736TruSeq Adapter, Index 25 (97% over 44bp)
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1510620.33199011638114817No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA1397450.3071186586546157No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1264310.2778583787066565No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC1244000.27339483442437434No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1197100.2630875854416547No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1187440.26096459983028863No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC1141030.2507650385235079No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG1139440.25041560300362464No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1103970.24262033389025445No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC1091720.2399281419917829No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT1014260.22290469836275395No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT969510.213069956529562No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG914770.20103970473182065No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT898470.19745744122609935No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT877920.19294115195968384No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG875650.19244227231808952No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT864260.18993908327942904No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT833880.18326244737121966No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA744460.16361054536621358No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC744320.16357977745880248No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC704060.1547318063704381No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC667300.14665303296735127No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT655440.14404655166809638No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA616950.13558757483771522No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG598800.13159873541263292No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA596900.1311811709549108No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC576220.1266363114887564No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA570560.12539240894627893No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA567570.12473529435228466No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT563110.12375511673047382No Hit
CTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGT558720.1227903230623685No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA556090.12221232594457421No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT534020.11736198511198101No Hit
GTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTG529410.11634884187508683No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG507460.11152487353456028No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG505370.11106555263106596No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT471050.10352301990000122No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT470610.10342632076242347No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT464950.10218241821994602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA213000.048.8809741
CTCCGCT37900.045.5132031
TCCGCTC33750.042.932542
CGGCAAT156300.040.4557231
TACGCAT86800.040.1131329
CTACGCA90700.038.3383458
CTCTACG94400.036.8854266
CCGAAAC346550.036.409951
TGCCGTC226850.034.18107250-51
ACTCGTC324950.034.0500342-43
TATGCCG229150.033.99345448-49
CGTCTTC227100.033.95521552-53
CCGTCTT230550.033.81793252-53
GCCGTCT233550.033.32252550-51
CCGCTCA41250.033.168753
ATGCCGT235900.032.8596948-49
CGACCGT3500.032.6746671
TCGTCTA344650.032.2002244-45
CTAACGA330400.032.16579494-95
CCAAACG386950.031.97997
Produced by FastQC (version 0.11.5)