FastQCFastQC Report
Fri 27 May 2016
SRR1233119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30351850
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT4325251.4250366946331112No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC3219081.0605877401212775No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT2655730.874981261438759No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT2519100.8299658834634462No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2161040.7119961386208749No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1409190.464284714111331No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG1397110.46030472607106315No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1365400.4498572574653604No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT1138170.3749919691880396No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA1076290.35460441455792646No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1001820.33006884259114355No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC978970.32254047117391527No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT927780.3056749423840721No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA913730.3010459000028005No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT882800.29085541737983023No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA793130.2613119134418495No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT791500.2607748786317803No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG749590.24696682409803683No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC737550.24300001482611436No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA708770.23351789100170173No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA693330.22843088642043236No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT681360.2244871399931141No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA630510.20773363073420567No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT617110.20331874333854444No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA604750.1992465039198599No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG603740.19891374001914214No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA598830.19729604620476182No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG596150.19641306872562958No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA581430.19156328197457484No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA559120.18421282393000757No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA503890.16601623953729344No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT493610.1626292960725623No Hit
GGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTGGTTGGCCCCCAA467210.15393130896469243No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT447330.14738146109709951No Hit
ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA441230.14537169892444776No Hit
GACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGA434850.14326968537337922No Hit
GGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCAC428360.14113143020936122No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT425890.14031764126404156No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG424250.13977731176188601No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA421020.13871312621800647No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG419270.1381365551029015No Hit
AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA415830.13700318102520934No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC410490.13524381545111747No Hit
TAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTC403220.13284857430436695No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA392310.129254065238198No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG382620.1260615086065594No Hit
CACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATAC381730.12576828101087742No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA378110.12457560247563164No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC372550.12274375367564087No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT369990.1219003125015444No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA366280.12067798173752177No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA366230.1206615082770902No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT355700.1171921975102012No Hit
AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA348770.11490897589438535No Hit
TACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTC347680.11454985445697707No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT345120.11370641328288063No Hit
GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC339270.11177901841238672No Hit
TAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATA333160.10976596154764866No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT332660.10960122694333295No Hit
GTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGT327430.10787810298219054No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT321420.10589799303831562No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT320880.10572007966565464No Hit
TATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGAC318950.10508420409299597No Hit
CCCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAA318660.10498865802249285No Hit
CTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAG316530.10428688860810792No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG314880.10374326441386604No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT305710.1007220317707158No Hit
GGCTTTTTATTGCTGGCATAGTGCGGGCATAGGGGTCAGGCAGAGTACTG304440.10030360587575386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT228850.082.738111
GATCGGG32600.068.821271
GCGTGTT290800.064.047432
ATCGGGA38450.058.1026462
GAGCGGC46150.057.3817869
CGAACGT43900.051.8661
CGTGTTT359700.051.405973
TCGAACG20650.043.828132
CGCCGGA37350.042.1763246-47
GCCGGAT46550.040.7106446-47
GTAGATA268400.039.292942
TCGGGAG51600.039.1394463
GTGTTTT474550.038.5067674
TAGATAT283400.037.413113
TCGGGGG74950.037.39052238-39
CGGGAGA57250.037.2674564
CCGGATC47600.035.79038248-49
AGATATA307600.034.959474
GCGCCGT14250.034.40939344-45
CTCGGGG68650.033.95594836-37
Produced by FastQC (version 0.11.5)