FastQCFastQC Report
Fri 27 May 2016
SRR1233119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30351850
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG3048651.0044363028942223No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT2492310.8211394033642102No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG2418850.796936595298145No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2112550.6960201766943366No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA2056640.6775995532397532No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA1983780.6535944266988668No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1717200.565764525061899No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1636110.539047866933976No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG1364390.44952449356464264No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT1307110.4306524972942341No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1287520.4241981954971444No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC1245930.41049557111016294No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC1056190.3479820834644346No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC1020620.3362628637134145No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1004520.33095840945444843No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC990490.32633595645734936No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT934450.3078725020056438No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA923160.3041527946401949No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC871030.28697756479423825TruSeq Adapter, Index 11 (100% over 50bp)
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT781170.25737146170661757No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT780070.257009045577123No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA720380.23734302851391265No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT708330.23337292454990388No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT707500.2330994651067398No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT687210.226414534863608No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA605980.19965175104647656No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT586560.19325345901485413No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT580080.19111849854292243No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA575240.1895238675731463No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG569630.18767554531272393No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC542010.17857560577032372No Hit
GTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTG540650.17812752764658496No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA534660.17615400708688267No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG518930.17097145643511022No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA467520.15403344441936817No Hit
ATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAG464890.1531669404006675No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC456870.1505245973474434No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA439850.1449170314165364No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA432250.14241306543093749No Hit
CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT417510.13755668929571013No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC415560.13691422433887884No Hit
CTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGT412460.1358928697921214No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA399300.13155705500653173No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT379800.12513240543821877No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT378480.12469750608282526No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG366650.12079988534471539No Hit
GTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCG366600.12078341188428382No Hit
AATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAA365310.12035839660514927No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC360840.11888566924256677No Hit
CCTCACTCTACTCAACCTCATAATCTATATTATTCTTACAGCCCCTATAT360440.11875388155911419No Hit
TATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGA357710.11785443061955038No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG348530.1148299032843138No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT348140.11470141029294754No Hit
CACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTAT344980.11366028759367221No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA336290.11079720017066505No Hit
CAATAAACTAGGAGGTGTCCTAGCCTTAATCTTATCTATCCTAATTTTAG331830.10932776750016886No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC331050.10907078151743634No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT327620.1079407021318305No Hit
AGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTC322340.10620110471025654No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC321480.10591776119083351No Hit
CTAATTTTAGCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCT318720.10500842617501074No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA313180.10318316675919259No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG312380.10291959139228746No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA311740.10270873109876333No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT305670.10070885300237052No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA303800.10009274558222975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA81900.049.1017271
TACGCAT138800.046.61089
CTACGCA141200.045.820978
CTCTACG143050.045.2280126
CTCCGCT24350.043.6359181
TCCGCTC21250.042.028132
CGGCAAT86850.040.268411
TCGTACC61450.037.1074032
CTCGTAC64350.035.8372841
CCCTCTC184100.035.5063322
CCCCTCT188100.034.5005951
CGTACCA66850.033.967823
TGCCGTC133700.033.89145348-49
CGTCTTC132800.033.60252450-51
TCCACTC667650.033.5699162
CCGCAAA54250.033.3750651
CTCCACT687350.033.1350941
ACTCGTC119800.033.0660642-43
GCCGTCT136350.032.97149748-49
TATGCCG138500.032.88834446-47
Produced by FastQC (version 0.11.5)