FastQCFastQC Report
Fri 27 May 2016
SRR1233118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34429118
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT2444700.7100675654833795No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1957300.568501348190215No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1933710.5616495897455172No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1702160.49439547071754786No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1517270.44069383363233416No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT1462550.4248003100166551No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1173690.3409003971580103No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1083540.3147161655433636No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA926310.269048425812128No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA889400.25832784911887663No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA833240.24201607488173238No Hit
GGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTGGTTGGCCCCCAA833160.2419928387360954No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA817360.23740369997279626No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG780600.22672669105261423No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC746610.21685423367511186No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT723920.21026388186882977No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA706470.20519549760176836No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT659230.19147455360314486No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT650380.18890405499205645No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT648470.18834929201497408No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT636990.18501490511607066No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG610200.17723370084589446No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG609580.17705362071720804No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT562580.16340238515549543No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT560660.16284471766020844No Hit
GGGTGTTCTACTGGTTGGCCCCCAATTCAGGTTAAGATAAGTAGGTTGGC529410.15376809827077184No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA527050.1530826319744816No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG515860.14983247610351216No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA509150.14788354438821233No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA501010.1455192665696519No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT494330.1435790484089659No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT487240.14151974500189055No Hit
CAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTAC471920.13707002311241318No Hit
CCCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAA467240.13571070859265114No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT460910.13387214856912685No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA418890.12166736307331485No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA417460.12125201697005424No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA413900.12021800848920963No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC411060.11939312531909763No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA408610.11868151835896579No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA407510.11836202135645764No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA398210.1156608194261613No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA395710.11493468987500638No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG388730.11290733616818184No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT381140.11070280685087548No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT369480.1073161386242889No Hit
TTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGT366590.10647673286315382No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT366550.10646511479033532No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA362880.10539915660923989No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG362290.10522779003516733No Hit
GTGGAATTATGTGAAGGGCTATTAGTGTTAGTTCTCGTGTGTGTGAGGGT352830.10248011581359708No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG349900.10162909197964352No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC349460.10150129317864023No Hit
ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA344310.1000054663032611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT573900.086.517761
GATCGGG181900.084.450871
GAGCGGC227300.082.81259
ATCGGGA187650.081.710812
TCGGGAG189450.073.0111853
GCGTGTT724500.067.810322
CGGGAGA219050.065.1584244
AGAGCGG308200.062.7000168
CGTGTTT842750.057.925543
GTGTTTT1001950.048.3584984
CGCCGGA257000.046.1396146-47
GCCGGAT302550.045.7148746-47
CCGGATC287500.044.88876748-49
GGAGAGC261950.044.5431336
TCGGGGG467950.043.74481638-39
GAGAGCG266350.043.6123857
TCTCGGG360750.043.5407736-37
CTCGGGG400750.043.30879636-37
GCGCCGG241500.042.6170944-45
GCGCCGT97000.042.3925144-45
Produced by FastQC (version 0.11.5)