FastQCFastQC Report
Fri 27 May 2016
SRR1233118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34429118
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC5921451.7198959322745357TruSeq Adapter, Index 8 (100% over 50bp)
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2673160.7764241883861214No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT2410900.700250293951765No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA2317190.6730320538562736No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1986010.5768402199556782No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1722640.500343924000609No Hit
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG1552660.45097292355848323No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1533250.4453352537233164No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA1362070.3956157110966363No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1360700.3952177921026033No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA1310590.38066325137925405No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG1181920.3432908156404123No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA1028590.2987558380089783No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1000050.2904663430529937No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC873980.25384908204735307No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA812710.23605309900764812No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT810880.23552157217620273No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC808540.2348419149163217No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT803980.23351745461501514No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC733840.21314516392781252No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT729990.21202692441903392No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA714250.2074552127649625No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG711640.20669713351355676No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG680430.19763213219693865No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC672380.19529399504221978No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT667260.1938068817214545No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA618490.17964154643752417No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT609470.17702167101695723No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA583410.16945249657571826No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC583380.1694437830211044No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT561070.16296380290659784No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT557270.16186008598884236No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT543990.1580028858131074No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT536140.1557228390224809No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA529710.15385523381691044No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT520890.15129344876043588No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT515120.1496175417563703No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG506180.14702090248144029No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA497730.14456658459853663No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC467060.135658427264968No Hit
AATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAA459810.1335526515666187No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT457140.13277714520598524No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT452110.13131617254906153No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC449030.13042158094203865No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA447750.13004980261184731No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC439250.12758096213792058No Hit
CCTCACTCTACTCAACCTCATAATCTATATTATTCTTACAGCCCCTATAT426260.12380799299011958No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG418460.12154246879051622No Hit
CTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGT414410.12036613891764524No Hit
CCGCAAACAAAGATCACCCAGCTACTACCATCATTCAAGTAGCACAACTA411880.11963129581187644No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG397770.11553302062515805No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA390950.1135521392096074No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT384080.11155673520303365No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG382240.11102230385338363No Hit
CCTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAAT356460.10353445592187405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC354430.10294483872633622TruSeq Adapter, Index 8 (98% over 50bp)
CAATAAACTAGGAGGTGTCCTAGCCTTAATCTTATCTATCCTAATTTTAG344980.10020006902297061No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT188900.047.5261049
CCCGAAA120750.047.436581
CTACGCA189150.047.365458
CTCTACG191050.046.6954156
CCGCAAA93900.046.28341
CGTCTTC757000.043.513350-51
GCCGTCT768300.043.0340548-49
ATGCCGT777100.042.56200846-47
TGCCGTC765350.042.343548-49
CCGTCTT770750.042.0899750-51
TATGCCG777100.041.87133446-47
CGCAAAC103750.041.2103732
CCCCTCT220850.040.7812421
GTATGCC822600.040.3839144-45
TCTCGTA765100.040.2869640-41
GCTTGAA831000.040.0814458-59
TGCTTGA823150.039.6760458-59
CGGCAAT78100.039.660521
CCCTCTC227650.039.6495362
CTCGTAT780450.038.9529442-43
Produced by FastQC (version 0.11.5)