FastQCFastQC Report
Fri 27 May 2016
SRR1233117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21516238
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1224660.5691794262547198No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT1016590.4724757181064831No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT838640.38977073966183123No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC816720.37958308510995276No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT803570.3734714219093505No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA730230.3393855375646988No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT688340.3199165207226282No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA612580.2847059044429607No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA526260.24458736699231529No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC510410.23722083758322435No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT509200.23665847161571646No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT470470.21865811300284No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA458530.21310881576974564No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT428350.19908220015041664No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA404410.1879557197684837No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG404000.18776516601089838No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT398080.18501375565747136No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA390570.18152336853682322No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA375390.1744682318535424No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG369500.1717307644579875No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT368410.171224170321968No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT316190.14695412831927215No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC315010.1464057053096364No Hit
GGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTGGTTGGCCCCCAA294670.136952379872355No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA282630.1313566061130203No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA276910.12869814881207392No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG271840.12634178893168962No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG265790.1235299590941502No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA258920.12033702174144012No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT258880.120318431130944No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT258680.12022547807846334No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT257110.1194957966164903No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG256430.1191797562380561No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT254860.11845007477608308No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT254700.11837571233409855No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA254050.11807361491353646No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA252410.1173113998831952No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC243250.11305415007958175No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG232470.10804398055087512No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT231950.10780230261442543No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT231580.10763033946733626No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG227210.1055993152706342No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG227110.10555283874439389No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT226920.10546453334453727No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG224820.10448852629349054No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA223720.103977284504847No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA222310.10332196548485846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT503500.087.113951
GATCGGG54200.070.9244841
ATCGGGA55750.068.525372
GAGCGGC59700.068.49679
GCGTGTT640350.067.5445562
TCGGGAG58950.059.146893
CGTGTTT748300.057.499083
CGGGAGA67950.051.7249954
GTGTTTT872750.048.6862034
CGCCGGA59000.045.48867846-47
GCCGGAT70650.043.75460446-47
CCGGATC69600.041.9070348-49
TCGAACG10450.041.480792
TCGGGGG109250.040.52437238-39
CGAACGT19350.040.372421
TCTCGGG86700.040.00163336-37
CTCGGGG95400.039.97912236-37
AGAGCGG108800.038.7766658
GCGCCGT21650.038.29366744-45
TCGCCGG15450.038.02244644-45
Produced by FastQC (version 0.11.5)