##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233117_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21516238
Sequences flagged as poor quality	0
Sequence length	101
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42742425511374	34.0	31.0	34.0	31.0	34.0
2	32.50375521036717	34.0	31.0	34.0	31.0	34.0
3	32.59322294166852	34.0	31.0	34.0	31.0	34.0
4	35.87116892832288	37.0	37.0	37.0	35.0	37.0
5	35.88013071801864	37.0	37.0	37.0	35.0	37.0
6	35.96140487012646	37.0	37.0	37.0	35.0	37.0
7	35.96291215034896	37.0	37.0	37.0	35.0	37.0
8	36.0587577159167	37.0	37.0	37.0	35.0	37.0
9	37.85641541983315	39.0	39.0	39.0	35.0	39.0
10-11	37.830395954906244	39.0	39.0	39.0	35.0	39.0
12-13	37.744219412334076	39.0	39.0	39.0	35.0	39.0
14-15	39.17562986615039	41.0	40.0	41.0	36.5	41.0
16-17	39.078910448936284	41.0	39.5	41.0	36.0	41.0
18-19	38.93311349316734	41.0	39.5	41.0	36.0	41.0
20-21	38.784017703280654	41.0	39.5	41.0	36.0	41.0
22-23	38.62513788423422	41.0	39.0	41.0	35.0	41.0
24-25	38.49310808887688	41.0	39.0	41.0	35.0	41.0
26-27	38.30331208457538	41.0	39.0	41.0	34.5	41.0
28-29	38.118272929496314	41.0	39.0	41.0	34.0	41.0
30-31	37.97028130568178	41.0	39.0	41.0	34.0	41.0
32-33	37.87527031909575	41.0	39.0	41.0	34.0	41.0
34-35	37.77428147522815	41.0	39.0	41.0	33.0	41.0
36-37	37.6668556789528	41.0	39.0	41.0	33.0	41.0
38-39	37.62865111456752	41.0	39.0	41.0	33.0	41.0
40-41	37.58458293220218	41.0	39.0	41.0	33.0	41.0
42-43	37.50163155845367	41.0	39.0	41.0	33.0	41.0
44-45	37.44403556978688	41.0	38.5	41.0	32.5	41.0
46-47	37.37478972857616	41.0	38.0	41.0	32.0	41.0
48-49	37.32353248741718	40.0	38.0	41.0	32.0	41.0
50-51	37.26343094922077	40.0	38.0	41.0	32.0	41.0
52-53	37.15288901805232	40.0	38.0	41.0	31.0	41.0
54-55	37.03044463441982	40.0	38.0	41.0	31.0	41.0
56-57	36.90849143795491	40.0	38.0	41.0	31.0	41.0
58-59	36.76023324802412	40.0	38.0	41.0	31.0	41.0
60-61	36.80181042801256	40.0	37.5	41.0	31.0	41.0
62-63	36.656224034145744	40.0	37.0	41.0	31.0	41.0
64-65	36.43862328535314	40.0	37.0	41.0	31.0	41.0
66-67	36.20786359120957	39.5	36.0	41.0	30.0	41.0
68-69	35.907397636148104	39.0	36.0	41.0	30.0	41.0
70-71	35.546383131660846	39.0	35.0	41.0	30.0	41.0
72-73	35.21807457697763	37.5	35.0	40.0	29.0	41.0
74-75	34.78429886302615	37.0	35.0	39.5	29.0	41.0
76-77	34.3885871684446	37.0	35.0	39.0	29.0	41.0
78-79	33.98685081471956	36.0	35.0	39.0	29.0	40.5
80-81	33.6066488481862	36.0	34.5	37.5	28.0	39.0
82-83	33.204129829759275	35.0	34.0	37.0	28.0	39.0
84-85	32.85457618102198	35.0	34.0	37.0	27.0	38.5
86-87	32.54133794206962	35.0	34.0	36.0	27.0	37.0
88-89	32.30036310250891	35.0	34.0	36.0	26.5	37.0
90-91	32.079756182284285	35.0	34.0	36.0	26.0	36.5
92-93	32.08478501213827	35.0	34.0	35.0	27.0	36.0
94-95	31.976933978886084	35.0	34.0	35.0	27.0	36.0
96-97	31.883612088693198	35.0	34.0	35.0	27.0	36.0
98-99	31.75639040616673	35.0	34.0	35.0	26.5	36.0
100-101	31.382778927245553	35.0	33.5	35.0	24.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.053924781551472734
1101	2	0.012289752553897415
1101	3	0.03328065824427995
1101	4	0.018681185453125693
1101	5	-0.003572199582187352
1101	6	0.05841502469155557
1101	7	0.04188634366795441
1101	8	0.05441147398391166
1101	9	0.08922327372953731
1101	10-11	0.12452166248046126
1101	12-13	0.19283253823542879
1101	14-15	0.22275550382104825
1101	16-17	0.15274911511689027
1101	18-19	0.04976354865796395
1101	20-21	0.036571864034868895
1101	22-23	0.04154719526142969
1101	24-25	0.009905011195563418
1101	26-27	1.9711437695946188E-4
1101	28-29	-0.011098404536667772
1101	30-31	-0.006147826224463415
1101	32-33	-0.050766282045792366
1101	34-35	-0.055873938211156826
1101	36-37	-0.03349317287934639
1101	38-39	-0.05445449961386828
1101	40-41	-0.02515782223653673
1101	42-43	-0.0420622893571192
1101	44-45	-0.04682241481462057
1101	46-47	-0.0335456346680445
1101	48-49	-0.05135264450852617
1101	50-51	-0.024767676606408884
1101	52-53	-0.05248617486417828
1101	54-55	-0.028846417061700436
1101	56-57	-0.008843591009366492
1101	58-59	-0.040064933981810213
1101	60-61	-0.03097609418729519
1101	62-63	-0.04021369944531017
1101	64-65	-0.024553364180121662
1101	66-67	-0.023914446368486608
1101	68-69	1.652412915404966E-4
1101	70-71	0.01883232297883808
1101	72-73	-0.02366091083632682
1101	74-75	-0.01530195572887294
1101	76-77	-0.04356038828203168
1101	78-79	-0.05530391150526981
1101	80-81	-0.07784459522140708
1101	82-83	-0.042660291222972546
1101	84-85	-0.04944308642063078
1101	86-87	-0.05188484938034321
1101	88-89	-0.06819523838842656
1101	90-91	-0.06491723704942132
1101	92-93	-0.09244763423312463
1101	94-95	-0.05032839149465573
1101	96-97	-0.0336799561677914
1101	98-99	0.004223113696820491
1101	100-101	0.057578754447064284
1102	1	0.055456500665236774
1102	2	0.03907084944651018
1102	3	0.03715428286746203
1102	4	0.03416176253901426
1102	5	0.04126540717572169
1102	6	0.1054989425286692
1102	7	0.04448546339150994
1102	8	0.08526372974113627
1102	9	0.1102499857780117
1102	10-11	0.14345976501601854
1102	12-13	0.18286820309884178
1102	14-15	0.18227379111880992
1102	16-17	0.11961449889304987
1102	18-19	0.06892886424044775
1102	20-21	0.057406312508803126
1102	22-23	0.09659855588625987
1102	24-25	0.09774080869561175
1102	26-27	0.08076884402134965
1102	28-29	0.05885952085156276
1102	30-31	0.06352450451640834
1102	32-33	0.041006112444996745
1102	34-35	0.04131752454123472
1102	36-37	0.056233873673512846
1102	38-39	0.04098205367429131
1102	40-41	0.043959111259546546
1102	42-43	0.08601600271117604
1102	44-45	0.08222565633445811
1102	46-47	0.07942171424625855
1102	48-49	0.10134746106652415
1102	50-51	0.09174100655871342
1102	52-53	0.0820523151690864
1102	54-55	0.09509374680391147
1102	56-57	0.10433398133332616
1102	58-59	0.09005162876677275
1102	60-61	0.09065006158453315
1102	62-63	0.09761649190063793
1102	64-65	0.10996285454233146
1102	66-67	0.13718823783583645
1102	68-69	0.15977061403474124
1102	70-71	0.1694949884538417
1102	72-73	0.1747048012657899
1102	74-75	0.12048574010798063
1102	76-77	0.10568185898122096
1102	78-79	0.11783864962280433
1102	80-81	0.10361295402213244
1102	82-83	0.08890766849016529
1102	84-85	0.09751541170045641
1102	86-87	0.09311418447948228
1102	88-89	0.10446921408362897
1102	90-91	0.11660817511473454
1102	92-93	0.10302113422785908
1102	94-95	0.09713817812328074
1102	96-97	0.13009488999425756
1102	98-99	0.13764720760938687
1102	100-101	0.18213669832856993
1103	1	0.04101869524142643
1103	2	0.04666545967040037
1103	3	0.06921138494722356
1103	4	0.030405879403637925
1103	5	0.02149351259438248
1103	6	0.09159172306260643
1103	7	0.056361326485351526
1103	8	0.08689782475405394
1103	9	0.09101442842781182
1103	10-11	0.12120131544099877
1103	12-13	0.1699991969359047
1103	14-15	0.14669735077890778
1103	16-17	0.05915355461725369
1103	18-19	0.03062954197677925
1103	20-21	0.03142493806552693
1103	22-23	0.07965604920961766
1103	24-25	0.03950900654681533
1103	26-27	0.05685412583680716
1103	28-29	0.023117149870266473
1103	30-31	0.018154342810262847
1103	32-33	0.01958264488790462
1103	34-35	0.03564413297780078
1103	36-37	0.021685691397344442
1103	38-39	0.0341843181175463
1103	40-41	0.024410790200860788
1103	42-43	0.03337035208441819
1103	44-45	0.040865738365141624
1103	46-47	0.027921425376980835
1103	48-49	0.03305106373027655
1103	50-51	0.05548228732664029
1103	52-53	0.019639410602337648
1103	54-55	0.08847358078017464
1103	56-57	0.04731351428662833
1103	58-59	0.058699843726031986
1103	60-61	0.04070835231037506
1103	62-63	0.05467761697961038
1103	64-65	0.05761943808365544
1103	66-67	0.07192249405132856
1103	68-69	0.05075543143320971
1103	70-71	0.06523218799918595
1103	72-73	0.09429749162207202
1103	74-75	0.0787507178493172
1103	76-77	0.09305603934006257
1103	78-79	0.09084691547521828
1103	80-81	0.09163655933048886
1103	82-83	0.08886618040700967
1103	84-85	0.04679242994748023
1103	86-87	0.0451002539119969
1103	88-89	0.08289154154167022
1103	90-91	0.07207384448305021
1103	92-93	0.08483256376486992
1103	94-95	0.06112038022933319
1103	96-97	0.09511619371075852
1103	98-99	0.1319376629712501
1103	100-101	0.143037943899035
1104	1	0.08394874370117122
1104	2	0.052735172466974234
1104	3	0.07983429936616204
1104	4	0.04958427858082359
1104	5	0.05467839606097158
1104	6	0.11269203773350966
1104	7	0.06167625659634268
1104	8	0.07505682672707081
1104	9	0.09780937171402826
1104	10-11	0.12396157777145334
1104	12-13	0.17239843712361136
1104	14-15	0.1486807567593189
1104	16-17	0.09047359671728117
1104	18-19	0.06644694579404131
1104	20-21	0.10019738962151337
1104	22-23	0.12521146075226852
1104	24-25	0.10917365931286582
1104	26-27	0.13692732675222885
1104	28-29	0.1255611374180532
1104	30-31	0.11774957817156917
1104	32-33	0.1384701521665619
1104	34-35	0.12798127662902914
1104	36-37	0.15974343409464353
1104	38-39	0.18959538924446662
1104	40-41	0.18314115100376682
1104	42-43	0.15924823875893424
1104	44-45	0.21095243833489974
1104	46-47	0.21209205046005764
1104	48-49	0.22108128408969918
1104	50-51	0.1813237288683638
1104	52-53	0.20018535027375606
1104	54-55	0.2104547438518054
1104	56-57	0.22326108529945543
1104	58-59	0.21052475770503065
1104	60-61	0.20223914764240192
1104	62-63	0.23384231885427909
1104	64-65	0.24322372693321626
1104	66-67	0.2593521139127333
1104	68-69	0.28495849256247396
1104	70-71	0.2501506997810097
1104	72-73	0.25696742711865284
1104	74-75	0.2763483416803325
1104	76-77	0.2901190915181857
1104	78-79	0.2895140038757589
1104	80-81	0.2354262527851958
1104	82-83	0.22256970064246673
1104	84-85	0.23319764857654945
1104	86-87	0.2373081572338407
1104	88-89	0.23956130767127348
1104	90-91	0.2414116939628812
1104	92-93	0.23870616634290798
1104	94-95	0.23837862691865297
1104	96-97	0.23726566912450764
1104	98-99	0.24518728978073412
1104	100-101	0.22793205570105712
1105	1	0.06906262313039235
1105	2	0.06840185448135827
1105	3	0.06611921122174635
1105	4	0.042853472090612854
1105	5	0.039156320303746384
1105	6	0.09016783542953988
1105	7	0.04030626447885055
1105	8	0.06057023831727548
1105	9	0.07665743451181584
1105	10-11	0.0836242049759619
1105	12-13	0.12566446706929213
1105	14-15	0.0855904840221342
1105	16-17	0.004479931582778818
1105	18-19	-0.013143395563929516
1105	20-21	0.003964783357758961
1105	22-23	0.003431980276310753
1105	24-25	0.03248645841498643
1105	26-27	0.03950137203700166
1105	28-29	0.04153560300196801
1105	30-31	0.036910042806596266
1105	32-33	0.06499272897602282
1105	34-35	0.07092458720271111
1105	36-37	0.11268395132032794
1105	38-39	0.10839219780078224
1105	40-41	0.10745934847524552
1105	42-43	0.09554790218095377
1105	44-45	0.09854871275893373
1105	46-47	0.13158378320910913
1105	48-49	0.15399828601204035
1105	50-51	0.12148244741513281
1105	52-53	0.14363749396618886
1105	54-55	0.13863227391193078
1105	56-57	0.1458756544246569
1105	58-59	0.12072771652297831
1105	60-61	0.13565804212221622
1105	62-63	0.17334814106162355
1105	64-65	0.16033429620225093
1105	66-67	0.15612722710690718
1105	68-69	0.14191101173491205
1105	70-71	0.13293091437888904
1105	72-73	0.17011537191659443
1105	74-75	0.2039993800263602
1105	76-77	0.1976548374302567
1105	78-79	0.1862656832616878
1105	80-81	0.1792081147575928
1105	82-83	0.1560930166791792
1105	84-85	0.1587295563859854
1105	86-87	0.18294998260509487
1105	88-89	0.1534767017616545
1105	90-91	0.17535588309825556
1105	92-93	0.13959503859452838
1105	94-95	0.12284465271665113
1105	96-97	0.12676868736095415
1105	98-99	0.11167182359413275
1105	100-101	0.16383793046482253
1106	1	0.11959391605959269
1106	2	0.09445200985338431
1106	3	0.0836594868882301
1106	4	0.05135235786547554
1106	5	0.0577956748157078
1106	6	0.10190931773185952
1106	7	0.024790301480429378
1106	8	0.04902053292338593
1106	9	0.08107996240862292
1106	10-11	0.09336306174334652
1106	12-13	0.136099975758178
1106	14-15	0.10725140702992064
1106	16-17	0.057756802551807596
1106	18-19	0.022791496281200807
1106	20-21	0.010748497390302703
1106	22-23	0.0674231489942585
1106	24-25	0.060515582515208166
1106	26-27	0.07264081362906438
1106	28-29	0.049964472590481535
1106	30-31	0.0499261762369656
1106	32-33	0.06044246868454195
1106	34-35	0.051010365173304706
1106	36-37	0.045183094572543325
1106	38-39	0.07532581244920067
1106	40-41	0.08639954074823208
1106	42-43	0.1269779061086922
1106	44-45	0.1141157349796913
1106	46-47	0.15228730362880327
1106	48-49	0.11957648809449495
1106	50-51	0.09328105229592865
1106	52-53	0.13031786838534742
1106	54-55	0.14695672766548284
1106	56-57	0.11630350815315182
1106	58-59	0.13477857102648727
1106	60-61	0.14606734070589056
1106	62-63	0.14475897084557232
1106	64-65	0.1550563082552685
1106	66-67	0.17982686544880266
1106	68-69	0.12338760386562342
1106	70-71	0.17481753628700858
1106	72-73	0.17342975381853876
1106	74-75	0.201621564196941
1106	76-77	0.2035249964023862
1106	78-79	0.21395685146530496
1106	80-81	0.2142956888661871
1106	82-83	0.25017128975277103
1106	84-85	0.22468605358927363
1106	86-87	0.20888924058097302
1106	88-89	0.2131025151669803
1106	90-91	0.22127413003980934
1106	92-93	0.21881347890588643
1106	94-95	0.21690524884901663
1106	96-97	0.22140995118113338
1106	98-99	0.19874820429283702
1106	100-101	0.22778528731839032
1107	1	0.04518424713849356
1107	2	0.057775702591648326
1107	3	0.028185662112463206
1107	4	0.03995629849983118
1107	5	0.03390299232815863
1107	6	0.06418364213526218
1107	7	0.0357414016513502
1107	8	0.05273319653412045
1107	9	0.06252517317542328
1107	10-11	0.08590736899618179
1107	12-13	0.09136135190938433
1107	14-15	0.04933201422839062
1107	16-17	-0.0456667254203893
1107	18-19	-0.07009658496394877
1107	20-21	-0.03915321639067315
1107	22-23	-0.024024681361602518
1107	24-25	-0.038801570100780225
1107	26-27	-0.01903432319782894
1107	28-29	0.002801432621915012
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2216	4	-0.19651266421178093
2216	5	-0.1409049635179258
2216	6	-0.18365249826567975
2216	7	-0.08716103302128886
2216	8	-0.1705670636294272
2216	9	-0.2665786450555956
2216	10-11	-0.2794256776502948
2216	12-13	-0.25345034303992264
2216	14-15	-0.3658274708074316
2216	16-17	-0.37577997857169265
2216	18-19	-0.34627181176222166
2216	20-21	-0.34717730068229713
2216	22-23	-0.36995061103361593
2216	24-25	-0.35240055382981694
2216	26-27	-0.34007882140046775
2216	28-29	-0.37339157217326857
2216	30-31	-0.37974480060525195
2216	32-33	-0.43377262136623074
2216	34-35	-0.4529855692594893
2216	36-37	-0.4782558729023165
2216	38-39	-0.5534256272498013
2216	40-41	-0.5029963217951234
2216	42-43	-0.5424929306112887
2216	44-45	-0.5577604153084295
2216	46-47	-0.5193293061212998
2216	48-49	-0.5019538986930172
2216	50-51	-0.5358208891221423
2216	52-53	-0.5304612749293938
2216	54-55	-0.5138257432924433
2216	56-57	-0.5849486334124663
2216	58-59	-0.5336896549307966
2216	60-61	-0.48573671848252076
2216	62-63	-0.5442890905432449
2216	64-65	-0.6173150912286474
2216	66-67	-0.519404524938615
2216	68-69	-0.46743557377307354
2216	70-71	-0.43094714099773057
2216	72-73	-0.42580822144175556
2216	74-75	-0.4765121968196553
2216	76-77	-0.498848442517307
2216	78-79	-0.4350520228690513
2216	80-81	-0.48099943959259406
2216	82-83	-0.44230068201785855
2216	84-85	-0.4319600681943214
2216	86-87	-0.43414592381787287
2216	88-89	-0.5050688351195589
2216	90-91	-0.561615534950608
2216	92-93	-0.44897511813293534
2216	94-95	-0.482841325254995
2216	96-97	-0.4615514444481761
2216	98-99	-0.47351505647836234
2216	100-101	-0.5002679983834746
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104491.0
3	40671.0
4	22561.0
5	19029.0
6	79505.0
7	82998.0
8	54519.0
9	67086.0
10	101670.0
11	133213.0
12	126815.0
13	84581.0
14	57252.0
15	47111.0
16	42770.0
17	39082.0
18	36597.0
19	37566.0
20	39670.0
21	45942.0
22	49329.0
23	57561.0
24	66218.0
25	81597.0
26	100927.0
27	117952.0
28	126377.0
29	130374.0
30	151052.0
31	188266.0
32	248303.0
33	341584.0
34	490491.0
35	752509.0
36	1349550.0
37	3449164.0
38	8262804.0
39	4274761.0
40	14290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.253791803884127	16.953792600371496	22.39002384198771	32.40239175375666
2	18.28242667317604	21.45320549730729	50.23968904497892	10.024678784537743
3	14.934988831462565	22.915852780919092	44.53774404889857	17.611414338719776
4	19.120470820233614	30.68315078974999	35.4385403940915	14.757837995924895
5	20.492379062169796	33.80890686344093	33.66308733107395	12.035626743315325
6	17.481833761830167	36.00376987924891	33.63127675314917	12.88311960577175
7	12.834213862107308	17.790837784932478	55.00805484676271	14.366893506197506
8	14.615877552572154	23.216312256817385	43.963498637633585	18.204311552976872
9	17.358950017191667	23.97150003639112	41.29855786127668	17.370992085140536
10-11	19.229704790293773	32.68962978463468	33.62631380244097	14.454351622630575
12-13	19.182756633527045	24.287634655637756	40.26002500070377	16.26958371013143
14-15	17.747496775406994	28.421631275443854	39.48489089789129	14.345981051257864
16-17	19.58638903289853	28.25259546402819	37.82219913926559	14.338816363807688
18-19	19.192121036046025	28.929197929184447	35.22943865778351	16.649242376986013
20-21	20.424455847101196	28.976611362423494	34.71688568962698	15.882047100848332
22-23	20.505705879377423	29.57832772624331	34.10827901005366	15.807687384325616
24-25	19.692532910525976	29.869947543272442	33.3846946861493	17.05282486005228
26-27	20.184826196186616	29.748756121151494	33.62767303165678	16.43874465100511
28-29	20.84071190244991	29.863332198303084	32.919544813704775	16.37641108554223
30-31	20.20996799560279	31.4932662076434	31.81832329786961	16.478442498884203
32-33	19.84881793956346	30.66424854018313	32.83676176231589	16.650171757937514
34-35	21.0029874902914	31.458966289659667	30.966266216698074	16.571780003350856
36-37	20.514686714987455	31.35371305169935	31.491258162176948	16.640342071136246
38-39	20.589894037183964	29.795988773937705	32.51419134268857	17.099925846189755
40-41	21.0416732606192	31.158940228708854	30.608727263712527	17.19065924695942
42-43	20.955241351735683	30.857571209371827	30.9730533463051	17.214134092587393
44-45	21.367928633483103	29.936728235201798	31.351300398406895	17.344042732908207
46-47	20.054669274979997	30.379609033937687	31.39033199993563	18.17538969114669
48-49	19.72298956882566	30.351831473521468	31.695421561568416	18.229757396084455
50-51	19.882866412018185	30.274292458320843	32.73408943337337	17.1087516962876
52-53	21.52195905733269	29.53430091291633	30.96540677569314	17.978333254057848
54-55	20.87720693461205	29.86354159633186	31.58558427591474	17.673667193141352
56-57	21.294155784315823	29.89418622293918	31.640583621041934	17.171074371703064
58-59	20.568606564574814	31.305205973016815	30.708163167054376	17.418024295353998
60-61	21.077822108635022	30.838405446001925	30.20132020324165	17.882452242121403
62-63	21.209039700084187	30.18664226506493	30.762704962441838	17.841613072409046
64-65	21.291182033176753	30.388165940354405	30.60253508148177	17.718116944987074
66-67	21.32377921116643	30.213655261779397	30.12393447240983	18.338631054644342
68-69	21.682000811575268	29.719402361922242	30.873443836826148	17.725152989676342
70-71	21.063759829115636	29.670529909427557	31.03390170417977	18.231808557277045
72-73	22.44413982716597	28.86718641057051	30.85587749410708	17.83279626815644
74-75	22.751290658116076	28.684818598186666	30.916913984385147	17.646976759312107
76-77	21.4505360397244	29.258452244802864	31.098660675447764	18.19235104002497
78-79	21.65830173632138	29.949579218855867	30.4061857101645	17.985933334658252
80-81	21.86213372127864	29.21845795478361	31.041505535922624	17.877902788015128
82-83	21.968084475159127	29.903954788006175	30.055621229012797	18.0723395078219
84-85	22.238879484327054	30.32231825537251	29.484914667707596	17.95388759259284
86-87	22.355171236691355	30.526554692305258	30.227598162589047	16.890675908414334
88-89	23.3787155109567	28.50524737249131	30.729930193308302	17.386106923243684
90-91	23.362087638110722	29.120195167317974	29.302251300606414	18.215465893964883
92-93	23.274322137980963	28.964882062952857	29.732880570325506	18.027915228740675
94-95	24.004902527650295	29.401096249127463	28.613223775017875	17.980777448204364
96-97	23.776043492850373	29.132210254409863	29.741555293663385	17.350190959076382
98-99	24.96144274042147	28.571727503794637	29.074128151382457	17.392701604401438
100-101	25.84215038806092	28.536709930541747	28.320040525684774	17.30109915571256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	671.0
1	660.0
2	699.0
3	906.5
4	1171.0
5	1673.5
6	2189.5
7	2684.0
8	3480.5
9	4405.0
10	5563.5
11	7173.5
12	9131.5
13	11265.5
14	14209.0
15	23757.0
16	44938.5
17	51463.0
18	54078.5
19	65118.0
20	73237.5
21	81517.5
22	89535.5
23	113217.5
24	148342.0
25	193457.0
26	247895.5
27	347096.0
28	460191.5
29	582773.5
30	694505.5
31	727119.0
32	814016.5
33	1020384.0
34	1233977.5
35	1237793.0
36	1057930.5
37	1033432.5
38	1058489.0
39	1063078.5
40	1096332.0
41	1038691.5
42	894303.0
43	762716.5
44	660037.0
45	526075.0
46	430807.0
47	385631.0
48	366095.0
49	342611.0
50	311713.5
51	287322.5
52	272383.0
53	258953.5
54	237066.5
55	205369.5
56	178013.5
57	160322.5
58	139253.0
59	106184.5
60	73949.5
61	52812.0
62	36565.5
63	25817.0
64	19731.0
65	14954.0
66	10469.5
67	6281.5
68	3409.5
69	2232.5
70	1796.0
71	2263.5
72	3428.5
73	4198.0
74	3822.5
75	2908.0
76	2607.0
77	2836.0
78	1937.5
79	647.5
80	229.0
81	112.5
82	79.5
83	50.5
84	40.0
85	33.0
86	27.5
87	26.0
88	17.5
89	14.0
90	14.5
91	14.0
92	12.0
93	13.0
94	14.0
95	12.0
96	9.5
97	9.5
98	11.5
99	11.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21830489140341355
2	0.5168793912764862
3	0.5430224372866669
4	0.5508258460424169
5	0.5637556156424743
6	0.061172403837511
7	0.0
8	0.0
9	0.0
10-11	0.02042410945630923
12-13	0.1154616341388304
14-15	0.2682439188486389
16-17	0.24442237532416217
18-19	0.3836962576822212
20-21	0.17347595801831156
22-23	0.1721188434520942
24-25	0.15561967663678009
26-27	0.12131535261879889
28-29	0.12249120873267902
30-31	0.10517173122922324
32-33	0.0915866426091773
34-35	0.08019989368029858
36-37	0.0761401691132065
38-39	0.06650093757096384
40-41	0.07486671229422169
42-43	0.08013482654356212
44-45	0.08303031412833414
46-47	0.07796902042076315
48-49	0.08493585170418733
50-51	0.08110850976829685
52-53	0.09049212041621775
54-55	0.12436653656647599
56-57	0.1411863914128483
58-59	0.17993851899202826
60-61	0.1869587982806288
62-63	0.1912276672158023
64-65	0.21068041727368886
66-67	0.2825168600570416
68-69	0.30378219463830064
70-71	0.34782102707731716
72-73	0.34576676461749495
74-75	0.39019367605061817
76-77	0.4192833338244353
78-79	0.49319727733073043
80-81	0.4942639136079458
82-83	0.522210248836251
84-85	0.5398829479391332
86-87	0.5483137897991275
88-89	0.6013667445024543
90-91	0.6321690622682273
92-93	0.6592579055873987
94-95	0.6692503587290678
96-97	0.6850291393876569
98-99	0.7180716257182134
100-101	0.7477724498120907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.1516238E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.656375210683542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01547255964545	21.32922459210062
2	8.579345387746573	4.573884994356399
3	3.361715089072591	2.688334162972063
4	1.8110162251202846	1.9310051203776817
5	1.143393411666906	1.5239361897408301
6	0.8165481122925555	1.3059727715307432
7	0.6184315640048356	1.1539600668569927
8	0.4534485509071686	0.966983576937778
9	0.35023277969253314	0.8402342747908348
>10	2.338866436439678	12.057257498118911
>50	0.25775205788161937	4.795916304458402
>100	0.20069347151088743	10.70474757687766
>500	0.026086528879605986	4.844835057072232
>1k	0.02176583448688729	11.72739868072522
>5k	0.0030694345162995606	5.66244130045425
>10k+	0.0021625561364837812	13.893867832629573
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	122466	0.5691794262547198	No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT	101659	0.4724757181064831	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	83864	0.38977073966183123	No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC	81672	0.37958308510995276	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	80357	0.3734714219093505	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	73023	0.3393855375646988	No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT	68834	0.3199165207226282	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	61258	0.2847059044429607	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	52626	0.24458736699231529	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	51041	0.23722083758322435	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	50920	0.23665847161571646	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	47047	0.21865811300284	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	45853	0.21310881576974564	No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT	42835	0.19908220015041664	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	40441	0.1879557197684837	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	40400	0.18776516601089838	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	39808	0.18501375565747136	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	39057	0.18152336853682322	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	37539	0.1744682318535424	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	36950	0.1717307644579875	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	36841	0.171224170321968	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	31619	0.14695412831927215	No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC	31501	0.1464057053096364	No Hit
GGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTGGTTGGCCCCCAA	29467	0.136952379872355	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	28263	0.1313566061130203	No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA	27691	0.12869814881207392	No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG	27184	0.12634178893168962	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	26579	0.1235299590941502	No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA	25892	0.12033702174144012	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	25888	0.120318431130944	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	25868	0.12022547807846334	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	25711	0.1194957966164903	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	25643	0.1191797562380561	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	25486	0.11845007477608308	No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT	25470	0.11837571233409855	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	25405	0.11807361491353646	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	25241	0.1173113998831952	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	24325	0.11305415007958175	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	23247	0.10804398055087512	No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT	23195	0.10780230261442543	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	23158	0.10763033946733626	No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG	22721	0.1055993152706342	No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG	22711	0.10555283874439389	No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT	22692	0.10546453334453727	No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG	22482	0.10448852629349054	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	22372	0.103977284504847	No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA	22231	0.10332196548485846	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017939939128764053	0.0	0.0	4.647652624032138E-6	0.0
2	0.0018032892181244695	0.0	0.0	4.647652624032138E-6	0.0
3	0.0018218798286205981	0.0	0.0	4.647652624032138E-6	0.0
4	0.0018404704391167266	0.0	0.0	4.647652624032138E-6	0.0
5	0.0018404704391167266	0.0	0.0	9.295305248064276E-6	0.0
6	0.001849765744364791	0.0	0.0	9.295305248064276E-6	0.0
7	0.001854413396988823	0.0	0.0	9.295305248064276E-6	0.0
8	0.0018590610496128552	0.0	0.0	9.295305248064276E-6	0.0
9	0.0018637087022368873	0.0	0.0	9.295305248064276E-6	0.0
10-11	0.0018683563548609196	0.0	0.0	9.295305248064276E-6	0.0
12-13	0.0019125090547892248	0.0	0.0	9.295305248064276E-6	0.0
14-15	0.001979900017837691	0.0	0.0	9.295305248064276E-6	0.0
16-17	0.0020403195019501087	2.323826312016069E-6	0.0	9.295305248064276E-6	0.0
18-19	0.0021053866386865584	6.971478936048206E-6	0.0	9.295305248064276E-6	0.0
20-21	0.0021774252543590566	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
22-23	0.0022610830015916353	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
24-25	0.0023726266645684065	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
26-27	0.002595713990521949	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
28-29	0.002930344979452263	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
30-31	0.0035786925205047466	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
32-33	0.005058969881258982	9.295305248064276E-6	0.0	9.295305248064276E-6	0.0
34-35	0.007580321429796417	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
36-37	0.011298443529022126	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
38-39	0.01704294217232585	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
40-41	0.025438926637639907	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
42-43	0.03676757990871825	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
44-45	0.051877098589446724	9.295305248064276E-6	0.0	1.3942957872096414E-5	0.0
46-47	0.07269625851879868	9.295305248064276E-6	0.0	1.859061049612855E-5	0.0
48-49	0.09872543703969067	9.295305248064276E-6	0.0	1.859061049612855E-5	0.0
50-51	0.12868652968051386	1.859061049612855E-5	0.0	2.323826312016069E-5	0.0
52-53	0.1689654111466884	1.859061049612855E-5	0.0	2.323826312016069E-5	0.0
54-55	0.22396805612579673	1.859061049612855E-5	0.0	2.323826312016069E-5	0.0
56-57	0.29008788618158987	1.859061049612855E-5	0.0	2.323826312016069E-5	0.0
58-59	0.36454560504489675	1.859061049612855E-5	0.0	2.556208943217676E-5	0.0
60-61	0.45552805281294995	2.323826312016069E-5	0.0	2.788591574419283E-5	0.0
62-63	0.5774057713992566	2.556208943217676E-5	0.0	2.788591574419283E-5	0.0
64-65	0.719772666578609	2.788591574419283E-5	0.0	2.788591574419283E-5	0.0
66-67	0.8816922363472648	2.788591574419283E-5	0.0	2.788591574419283E-5	0.0
68-69	1.0735101554463191	3.0209742056208894E-5	0.0	2.788591574419283E-5	0.0
70-71	1.2890682841489296	3.2533568368224964E-5	0.0	2.788591574419283E-5	0.0
72-73	1.5428231459421484	3.2533568368224964E-5	0.0	2.788591574419283E-5	0.0
74-75	1.8576783729572055	3.2533568368224964E-5	0.0	2.788591574419283E-5	0.0
76-77	2.208120676114477	3.2533568368224964E-5	0.0	2.788591574419283E-5	0.0
78-79	2.615036606306363	3.71812209922571E-5	0.0	2.788591574419283E-5	0.0
80-81	3.0896084157462846	3.71812209922571E-5	0.0	2.788591574419283E-5	0.0
82-83	3.638054663645197	3.71812209922571E-5	0.0	2.788591574419283E-5	0.0
84-85	4.3236554643056095	3.71812209922571E-5	0.0	3.0209742056208894E-5	0.0
86-87	5.07745591957107	3.71812209922571E-5	0.0	3.2533568368224964E-5	0.0
88-89	5.908625848068794	3.71812209922571E-5	0.0	3.71812209922571E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	50350	0.0	87.11395	1
GATCGGG	5420	0.0	70.924484	1
ATCGGGA	5575	0.0	68.52537	2
GAGCGGC	5970	0.0	68.4967	9
GCGTGTT	64035	0.0	67.544556	2
TCGGGAG	5895	0.0	59.14689	3
CGTGTTT	74830	0.0	57.49908	3
CGGGAGA	6795	0.0	51.724995	4
GTGTTTT	87275	0.0	48.686203	4
CGCCGGA	5900	0.0	45.488678	46-47
GCCGGAT	7065	0.0	43.754604	46-47
CCGGATC	6960	0.0	41.90703	48-49
TCGAACG	1045	0.0	41.48079	2
TCGGGGG	10925	0.0	40.524372	38-39
CGAACGT	1935	0.0	40.37242	1
TCTCGGG	8670	0.0	40.001633	36-37
CTCGGGG	9540	0.0	39.979122	36-37
AGAGCGG	10880	0.0	38.776665	8
GCGCCGT	2165	0.0	38.293667	44-45
TCGCCGG	1545	0.0	38.022446	44-45
>>END_MODULE