FastQCFastQC Report
Fri 27 May 2016
SRR1233117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21516238
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1237590.5751888410975934TruSeq Adapter, Index 1 (100% over 50bp)
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1231370.5722980011654454No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1041090.48386246703536184No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1014360.4714392915713239No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA874850.4065998898134516No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC794870.3694279641264426No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT755370.3510697362615156No Hit
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG627090.29144964840043136No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT613710.2852310891894764No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC548460.25490515581766665No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA536480.24933726797407613No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA529500.24609320644250168No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA511070.2375275826564105No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT510950.23747181082492208No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG480820.22346843346871323No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT457570.21266264111783856No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC421850.19606122594479575No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA406750.18904327048250721No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT393870.18305709390275382No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA366540.170355059281274No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC343970.15986530730883344No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG337250.15674208474548385No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC334030.15524554060054552No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC318990.14825547105400116No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT315760.1467542792564388No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC297610.13831878974382045No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC288860.13425209369779234No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC287950.1338291573090054No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT280640.1304317232408379No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC279870.13007385398878746No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG270210.1255842215539724No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA267720.1244269560505884No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC262850.12216354922268474No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT254500.11828275928161792No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA251930.11708831255724166No Hit
CTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGT249600.11600540949584215No Hit
CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT249320.11587527522236928No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA245590.11414170079360526No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG235010.10922448431737927No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA228070.10599901339630098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCT16100.051.4948731
CCCGAAA115450.049.731751
TCCGCTC14650.046.3721472
TACGCAT41400.042.3376059
CGGCAAT45550.041.6327741
CTCTACG41900.041.040536
CTACGCA42400.040.6691638
TGCCGTC174850.040.47553648-49
CGTCTTC172800.040.2410550-51
TATGCCG176500.039.98950646-47
CCGTCTT176850.039.91036650-51
GCCGTCT177450.039.48098448-49
ATGCCGT179650.039.05037746-47
CCGCAAA63500.036.0920871
CGCAAAC62400.035.782612
TCTCGTA173850.035.4217340-41
CTCCACT428050.035.0079571
TCCACTC423950.034.5699922
GTATGCC205100.034.28583544-45
ACTCGTC165650.034.10711742-43
Produced by FastQC (version 0.11.5)