FastQCFastQC Report
Fri 27 May 2016
SRR1233116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96592502
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC2912820.30155756810192164No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG2543560.2633289279534347No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1885410.19519216926382132No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT1780490.1843300425119954No Hit
GTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA1509710.15629681069861923No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1453780.15050650618823394No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1370660.14190128339361163No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1345060.13925097415946425No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1309780.1355985167461549No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1258980.13033930935964366No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1229800.1273183709435335No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1221130.12642078574587495No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1018910.10548541335019979No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG991910.10269016532980996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG358800.040.900531
TCACGGT586450.028.4877512
CGGCGAG77050.028.4834141
CACGGTC660850.025.0596393
GCGACGG28900.024.3146861
CGACGGC124100.023.96231
GACGGCC176750.023.4214672
TTGGCGA120500.022.7343541
TTACGCG122050.021.3768528
GGCCAGT689100.021.1702162
ACGGCCA202100.020.4175033
TACGCGC131550.019.9773569
CTGGAAT2867550.019.6679611
CGGACAG113450.018.8957881
CGACGGA25400.018.4434221
GCCAGTG798150.018.424543
ACGGTCT941950.017.4473174
CTTTTAT2386300.016.8381521
TGAATTG1324750.016.4202672
TATTATA2160050.016.3381799
Produced by FastQC (version 0.11.5)