##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96592502 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.568388444891923 31.0 27.0 33.0 10.0 34.0 2 26.83466751901716 31.0 27.0 34.0 2.0 34.0 3 27.34942235992603 31.0 27.0 34.0 10.0 34.0 4 30.77195558098288 35.0 30.0 37.0 12.0 37.0 5 30.996646447775003 35.0 31.0 37.0 16.0 37.0 6 31.151717821741485 35.0 31.0 37.0 17.0 37.0 7 30.53848919867507 35.0 32.0 37.0 7.0 37.0 8 30.72100536333555 35.0 32.0 37.0 10.0 37.0 9 32.02333682173384 37.0 32.0 39.0 10.0 39.0 10-11 32.159376759906266 37.0 31.0 39.0 14.0 39.0 12-13 32.22724831685176 37.0 31.0 39.0 12.0 39.0 14-15 32.88485815907326 38.0 31.0 40.0 10.5 41.0 16-17 33.095938331735105 38.0 31.0 40.0 13.5 41.0 18-19 33.07191358393429 38.0 31.0 40.0 11.5 41.0 20-21 33.066344083311975 38.0 31.0 40.0 10.5 41.0 22-23 32.97374852139144 38.0 31.0 40.0 10.5 41.0 24-25 32.82970504273717 38.0 31.0 40.0 10.0 41.0 26-27 32.715618946282184 38.0 31.0 40.0 10.0 41.0 28-29 32.67132840704343 38.0 30.5 40.0 10.0 41.0 30-31 32.498971343552114 38.0 30.0 40.0 9.0 41.0 32-33 32.25855593325453 38.0 30.0 40.0 8.0 41.0 34-35 32.066204688434304 37.0 30.0 40.0 8.0 41.0 36-37 32.00180486576484 37.0 30.0 40.0 8.0 41.0 38-39 31.863051326696144 37.0 30.0 40.0 7.0 41.0 40-41 31.74610964109823 37.0 30.0 40.0 4.5 41.0 42-43 31.547927534789398 37.0 30.0 40.0 2.0 41.0 44-45 31.650277973957024 37.0 30.0 40.0 2.0 41.0 46-47 31.64024996474364 37.0 30.0 40.0 2.0 41.0 48-49 31.565187368270056 37.0 30.0 40.0 2.0 41.0 50-51 31.20740722711583 36.0 29.5 39.5 2.0 40.5 52-53 31.21203765381292 37.0 30.0 39.5 2.0 40.5 54-55 31.435329167682184 37.0 30.0 40.0 2.0 41.0 56-57 31.440561136929656 37.0 30.0 40.0 2.0 41.0 58-59 31.29905455808568 37.0 29.5 40.0 2.0 41.0 60-61 31.03856526565592 36.0 29.0 40.0 2.0 41.0 62-63 30.818421817047454 36.0 29.0 40.0 2.0 41.0 64-65 30.595137912464466 36.0 29.0 39.5 2.0 41.0 66-67 30.328289192674603 35.5 29.0 39.0 2.0 41.0 68-69 29.943249523653503 35.0 28.0 39.0 2.0 40.5 70-71 29.588584701947156 35.0 28.0 39.0 2.0 40.0 72-73 29.12119250726107 34.5 28.0 38.0 2.0 40.0 74-75 28.679166298021766 34.0 27.0 37.0 2.0 40.0 76-77 28.26843899850529 34.0 26.0 37.0 2.0 39.0 78-79 27.837114318666266 34.0 26.0 36.0 2.0 39.0 80-81 27.372051870030244 34.0 26.0 36.0 2.0 38.5 82-83 27.140623756696975 34.0 26.0 35.5 2.0 37.0 84-85 26.86779225368859 33.5 25.5 35.0 2.0 37.0 86-87 26.503254315743888 33.0 25.0 35.0 2.0 36.5 88-89 26.146965014944946 33.0 23.5 35.0 2.0 36.0 90-91 25.850445845165083 33.0 22.5 35.0 2.0 36.0 92-93 25.586439711438473 33.0 20.5 35.0 2.0 35.5 94-95 25.30248945720445 33.0 19.5 35.0 2.0 35.0 96-97 24.971872702914354 32.0 14.0 35.0 2.0 35.0 98-99 24.552372191373614 32.0 2.0 35.0 2.0 35.0 100-101 22.971294516214105 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.854469361011915 1101 2 6.0271491264198715 1101 3 5.353038348536842 1101 4 5.621659896130343 1101 5 5.343308925786445 1101 6 5.131811875817512 1101 7 6.394923405366093 1101 8 6.074993126780548 1101 9 6.290873577599875 1101 10-11 5.69591997333217 1101 12-13 5.510100662824851 1101 14-15 6.152569360438747 1101 16-17 5.687482584812209 1101 18-19 5.680777719988672 1101 20-21 5.648570107496031 1101 22-23 5.482063013976994 1101 24-25 5.6158726202750735 1101 26-27 5.506106552859777 1101 28-29 5.360258273925616 1101 30-31 5.403212239428505 1101 32-33 5.6633465507615846 1101 34-35 5.535145330007836 1101 36-37 5.504362120207343 1101 38-39 5.530233490362328 1101 40-41 5.414863805160923 1101 42-43 5.471972232158553 1101 44-45 5.413971871745417 1101 46-47 5.18284805437283 1101 48-49 5.087504934512438 1101 50-51 5.021378813666512 1101 52-53 5.163272504150857 1101 54-55 5.429008009204349 1101 56-57 5.329707692957484 1101 58-59 5.2929677059653635 1101 60-61 5.230519047372841 1101 62-63 5.157031199280482 1101 64-65 5.250266658325451 1101 66-67 5.249371569652464 1101 68-69 5.27770895988267 1101 70-71 5.212447333193214 1101 72-73 5.28930724311914 1101 74-75 5.305999719889272 1101 76-77 5.240102939264403 1101 78-79 5.341959176278564 1101 80-81 5.4678347167239245 1101 82-83 5.220379323792084 1101 84-85 5.210304423798554 1101 86-87 5.125477319131768 1101 88-89 5.100660654883399 1101 90-91 5.06624514021879 1101 92-93 5.04846578388851 1101 94-95 5.018662737770324 1101 96-97 4.899721432683762 1101 98-99 4.9039467399233985 1101 100-101 4.570214628913636 1102 1 4.6463201904568585 1102 2 5.843082957650616 1102 3 5.219841351931471 1102 4 5.3954576270737284 1102 5 5.082192509835167 1102 6 5.043738877313608 1102 7 6.273286150965184 1102 8 6.035709891450075 1102 9 6.144812977691323 1102 10-11 5.602222478482712 1102 12-13 5.438744113740459 1102 14-15 6.091776978746566 1102 16-17 5.683856038239398 1102 18-19 5.6103252238299355 1102 20-21 5.511175526513252 1102 22-23 5.371687337882815 1102 24-25 5.525600255596668 1102 26-27 5.36191330514653 1102 28-29 5.2354146745837795 1102 30-31 5.290318530316888 1102 32-33 5.538125157425231 1102 34-35 5.466751588431016 1102 36-37 5.388505362174126 1102 38-39 5.474985021379823 1102 40-41 5.3686956943623905 1102 42-43 5.481646133128752 1102 44-45 5.333957232687311 1102 46-47 5.112832394355269 1102 48-49 5.0949349589266575 1102 50-51 4.943020695377999 1102 52-53 5.178279241590776 1102 54-55 5.423849251051838 1102 56-57 5.440325154146734 1102 58-59 5.293309241116507 1102 60-61 5.2015246453300925 1102 62-63 5.1445982788115 1102 64-65 5.201485205606275 1102 66-67 5.193310408770632 1102 68-69 5.278451704768383 1102 70-71 5.209378636850726 1102 72-73 5.284861511320258 1102 74-75 5.3252494657521225 1102 76-77 5.327630467472552 1102 78-79 5.251596406894905 1102 80-81 5.27755479103195 1102 82-83 5.223920211779308 1102 84-85 5.1634441271384794 1102 86-87 5.11591217589789 1102 88-89 5.149230332652905 1102 90-91 5.052537901176571 1102 92-93 5.047120067406368 1102 94-95 5.117776587714271 1102 96-97 5.038470759281086 1102 98-99 4.9635224120365535 1102 100-101 4.551745676582154 1103 1 4.4362790848184765 1103 2 5.616490328713336 1103 3 5.0533425448003335 1103 4 5.2020348620176655 1103 5 4.950967176897102 1103 6 4.867102712394619 1103 7 6.088086455165119 1103 8 5.8201769826165375 1103 9 5.968199594145574 1103 10-11 5.47281623254619 1103 12-13 5.362890455760098 1103 14-15 5.946130680118223 1103 16-17 5.404397712622512 1103 18-19 5.237893804807161 1103 20-21 5.148421082573048 1103 22-23 5.105666014716828 1103 24-25 5.358497599636582 1103 26-27 5.276010303984052 1103 28-29 5.161413409033507 1103 30-31 5.226109684358242 1103 32-33 5.4567645878732485 1103 34-35 5.355136990717384 1103 36-37 5.2280848275286935 1103 38-39 5.267780027020066 1103 40-41 5.082671140488646 1103 42-43 5.151291457815095 1103 44-45 5.048758054225843 1103 46-47 4.949955432881364 1103 48-49 4.999603088699445 1103 50-51 4.888944396169979 1103 52-53 5.063366457682736 1103 54-55 5.238080852076354 1103 56-57 5.193217582125328 1103 58-59 5.172631155827279 1103 60-61 5.019919957341802 1103 62-63 4.883261798973514 1103 64-65 5.002478370751831 1103 66-67 5.078962353417989 1103 68-69 5.101148038215722 1103 70-71 5.016439381807377 1103 72-73 5.0438238680598175 1103 74-75 4.979759534349533 1103 76-77 5.03006666147348 1103 78-79 5.088271019278835 1103 80-81 5.187135997869635 1103 82-83 5.01545557260749 1103 84-85 5.001557742548162 1103 86-87 4.85309675212784 1103 88-89 4.8980310249073185 1103 90-91 4.936808306027391 1103 92-93 4.856933901452294 1103 94-95 4.890886739146271 1103 96-97 4.884866332878527 1103 98-99 4.730995842234332 1103 100-101 4.344262766066073 1104 1 4.409802569302812 1104 2 5.683397857778505 1104 3 4.972421943042978 1104 4 5.135886185653792 1104 5 4.876366052142814 1104 6 4.7572335276477915 1104 7 6.102637237304421 1104 8 5.894083062488477 1104 9 5.972505456674554 1104 10-11 5.380274857293781 1104 12-13 4.952533595929932 1104 14-15 5.797455133127009 1104 16-17 5.368848634665518 1104 18-19 5.256988281608859 1104 20-21 5.2044386271027285 1104 22-23 5.174402962763988 1104 24-25 5.333817897744726 1104 26-27 5.145679919718056 1104 28-29 5.001816972619018 1104 30-31 5.031638242483879 1104 32-33 5.184868640373242 1104 34-35 5.245853381030713 1104 36-37 5.1501069000172315 1104 38-39 5.250067999078972 1104 40-41 5.036056943716421 1104 42-43 5.246897193263134 1104 44-45 5.010880865281983 1104 46-47 4.7684152128183435 1104 48-49 4.7465409193124515 1104 50-51 4.578991352349679 1104 52-53 4.6745719102005445 1104 54-55 5.000401382347722 1104 56-57 5.03184011416915 1104 58-59 4.910048145883298 1104 60-61 4.764742308152432 1104 62-63 4.657718977419513 1104 64-65 4.753159361898511 1104 66-67 4.700961909244004 1104 68-69 4.9015115923527155 1104 70-71 4.856416143923152 1104 72-73 4.910568610136625 1104 74-75 4.93257167397033 1104 76-77 4.926273289832501 1104 78-79 4.91073452229714 1104 80-81 4.916700851527967 1104 82-83 4.8906728733579605 1104 84-85 4.9314588051685355 1104 86-87 4.856088185733544 1104 88-89 4.908255666510897 1104 90-91 4.854657781306642 1104 92-93 4.825059148148668 1104 94-95 4.773580853267692 1104 96-97 4.715063163340389 1104 98-99 4.699919351924148 1104 100-101 4.411849205852246 1105 1 4.215145082687872 1105 2 5.505579387746415 1105 3 4.77780895732997 1105 4 5.033427524507783 1105 5 3.6857487535703655 1105 6 4.232921540163069 1105 7 4.807941393818929 1105 8 5.278918951072001 1105 9 5.345959429065132 1105 10-11 4.869599050229969 1105 12-13 4.50797758258334 1105 14-15 4.377137298677809 1105 16-17 4.531766644475468 1105 18-19 4.862609355154039 1105 20-21 4.881872832439452 1105 22-23 4.693034167069836 1105 24-25 4.9611810518140835 1105 26-27 4.866001292957193 1105 28-29 4.754204966426435 1105 30-31 4.849521685413578 1105 32-33 5.014835042023957 1105 34-35 4.962514284667378 1105 36-37 4.8545495879814275 1105 38-39 4.997091257153421 1105 40-41 4.806142930706027 1105 42-43 5.052216783626623 1105 44-45 4.780534354968566 1105 46-47 4.647666874919633 1105 48-49 4.642341171173243 1105 50-51 4.4638696480108315 1105 52-53 4.657315467312163 1105 54-55 4.771234071286688 1105 56-57 4.736511481997567 1105 58-59 4.717370999170061 1105 60-61 4.669190964796616 1105 62-63 4.618056943700282 1105 64-65 4.611512759059863 1105 66-67 4.657330991677174 1105 68-69 4.717729375729871 1105 70-71 4.718762711391168 1105 72-73 4.715066311157059 1105 74-75 4.737063056193492 1105 76-77 4.591807911838657 1105 78-79 4.673280332552814 1105 80-81 4.70812874830747 1105 82-83 4.577072974133191 1105 84-85 4.572953658072702 1105 86-87 4.416932994204721 1105 88-89 4.4160755272345895 1105 90-91 4.362830661100155 1105 92-93 4.311930575580163 1105 94-95 4.283173816516875 1105 96-97 4.153097373592807 1105 98-99 4.2112184511818676 1105 100-101 3.8730253517364908 1106 1 4.21776718764681 1106 2 5.371875919484932 1106 3 4.585108983109013 1106 4 4.82490401638621 1106 5 4.641329638793721 1106 6 4.4245303739343385 1106 7 5.666043165452869 1106 8 5.056443570353416 1106 9 5.297815528999088 1106 10-11 3.7063328977187915 1106 12-13 4.70836849044672 1106 14-15 5.25758158818266 1106 16-17 4.832947817922708 1106 18-19 4.73513830818457 1106 20-21 4.825604313163392 1106 22-23 4.679365491671948 1106 24-25 4.858635191847579 1106 26-27 4.7045968049642966 1106 28-29 4.700698735557523 1106 30-31 4.479816865239513 1106 32-33 4.7504574374729565 1106 34-35 4.198025090078495 1106 36-37 4.445042969808565 1106 38-39 4.726553504337453 1106 40-41 4.63154042211718 1106 42-43 4.63570827158475 1106 44-45 4.5299191463461455 1106 46-47 4.3653517117464595 1106 48-49 4.384715494562094 1106 50-51 4.171719724125413 1106 52-53 4.357549408206726 1106 54-55 4.512649910002811 1106 56-57 4.308901984599444 1106 58-59 4.320983008984573 1106 60-61 4.185060577328617 1106 62-63 4.20746929331613 1106 64-65 4.303488603240645 1106 66-67 4.397217652965896 1106 68-69 4.3964323503601825 1106 70-71 4.265351217746979 1106 72-73 4.343943960278192 1106 74-75 4.468869377012197 1106 76-77 4.450533427281805 1106 78-79 4.542707696218031 1106 80-81 4.6464956407239875 1106 82-83 4.467319929397295 1106 84-85 4.47037735386894 1106 86-87 4.439006771196588 1106 88-89 4.430436158744328 1106 90-91 4.424286790795811 1106 92-93 4.376555208641619 1106 94-95 4.330305474515544 1106 96-97 4.335475937927185 1106 98-99 4.273579187826645 1106 100-101 4.073729830533349 1107 1 1.0899994053093565 1107 2 1.8731591946040602 1107 3 1.0714719939438986 1107 4 1.5357272985241721 1107 5 1.1645306202911918 1107 6 1.7567531324725394 1107 7 1.865383642654475 1107 8 1.724523782286763 1107 9 2.60704669250525 1107 10-11 2.1395267052355713 1107 12-13 1.346040998561282 1107 14-15 2.291039318801694 1107 16-17 2.6086648788655893 1107 18-19 2.5307511257022846 1107 20-21 0.741077546105462 1107 22-23 1.263092522273535 1107 24-25 0.9564789330513044 1107 26-27 1.1542222031112246 1107 28-29 1.1704683097720157 1107 30-31 1.128411364712548 1107 32-33 1.6484172361005598 1107 34-35 0.7928085213176104 1107 36-37 1.3282267528903162 1107 38-39 0.8907439733802924 1107 40-41 1.8334900272766 1107 42-43 1.8524431154385859 1107 44-45 1.2359869311711478 1107 46-47 2.2404724465026042 1107 48-49 2.559954692071564 1107 50-51 2.533830271464968 1107 52-53 1.626787734944262 1107 54-55 1.6178981191453055 1107 56-57 1.8368751134814687 1107 58-59 1.6524140324216496 1107 60-61 1.5346739365658841 1107 62-63 1.3753495969688778 1107 64-65 1.1022881184963254 1107 66-67 0.7656371000014062 1107 68-69 1.3734402457934145 1107 70-71 1.4375607001071948 1107 72-73 2.0879135834982314 1107 74-75 2.3928254295201583 1107 76-77 2.354131525562977 1107 78-79 2.255883254059075 1107 80-81 1.693200018761413 1107 82-83 1.9531538309652845 1107 84-85 1.8440564438684248 1107 86-87 1.9736995742544536 1107 88-89 1.5941731803569539 1107 90-91 1.9993689339349494 1107 92-93 2.026558054843562 1107 94-95 2.0361886889478704 1107 96-97 1.6968336334984997 1107 98-99 1.4104538662153452 1107 100-101 1.8270843688868332 1108 1 0.7270888732923098 1108 2 0.015159404775385354 1108 3 0.4027622973829743 1108 4 -1.3989491918904164 1108 5 -0.40844444368692834 1108 6 -1.742254422196119 1108 7 -1.0663676572935117 1108 8 -1.2989957881243726 1108 9 -3.727276826451 1108 10-11 -1.6203060772775828 1108 12-13 -0.6659233678674035 1108 14-15 0.3753077609081359 1108 16-17 -1.798911517007582 1108 18-19 -1.404231564822986 1108 20-21 -0.15311500732779848 1108 22-23 -0.5179907655674789 1108 24-25 -2.4555762520537705 1108 26-27 -1.8356093579002248 1108 28-29 -1.6074070090672734 1108 30-31 -1.0123653768708536 1108 32-33 -1.5646380320499205 1108 34-35 -0.44365016863001827 1108 36-37 0.09866620107689883 1108 38-39 -1.727616658060434 1108 40-41 -1.7250934269891154 1108 42-43 -1.211013316359665 1108 44-45 -0.7898711836013774 1108 46-47 -0.4560650552601899 1108 48-49 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-0.2182824414492437 2312 68-69 -0.7252893829423037 2312 70-71 -0.013831820860396249 2312 72-73 -0.49596125223567 2312 74-75 0.2752791812935911 2312 76-77 0.5661189829171462 2312 78-79 0.4046887032597155 2312 80-81 0.672550435195955 2312 82-83 0.8570098399201065 2312 84-85 -0.6105118949499833 2312 86-87 -0.47779893839306453 2312 88-89 -0.04712462377548121 2312 90-91 0.21372137262537194 2312 92-93 0.6059294711618222 2312 94-95 0.29726309053757305 2312 96-97 -0.12783478009777838 2312 98-99 -0.29943588224889695 2312 100-101 -0.9014274615516484 2313 1 -4.404487358173995 2313 2 -22.681953744955663 2313 3 -12.863114416835792 2313 4 -9.857522499311077 2313 5 -6.805468797641716 2313 6 -6.124383497352564 2313 7 -26.15741301395186 2313 8 -13.74701968533084 2313 9 -10.224187774668472 2313 10-11 -7.48683229365103 2313 12-13 -6.344968729589219 2313 14-15 -6.63238183617171 2313 16-17 -7.813869157173961 2313 18-19 -9.233975687394608 2313 20-21 -7.193390262004986 2313 22-23 -8.310949235981557 2313 24-25 -11.280430128111501 2313 26-27 -10.105582990140828 2313 28-29 -8.118081239689328 2313 30-31 -7.628986932034209 2313 32-33 -7.782118734654734 2313 34-35 -7.70369055243868 2313 36-37 -9.505190654233466 2313 38-39 -9.560063195472061 2313 40-41 -8.249548853577043 2313 42-43 -8.047202019754142 2313 44-45 -7.964425682625556 2313 46-47 -7.996131327335728 2313 48-49 -8.143045237766394 2313 50-51 -8.243446243194853 2313 52-53 -8.143107526237845 2313 54-55 -8.378459044950642 2313 56-57 -8.510818692423328 2313 58-59 -9.034387647210256 2313 60-61 -8.834757628655392 2313 62-63 -10.398734311357373 2313 64-65 -10.156403442652206 2313 66-67 -10.046817542025497 2313 68-69 -10.408454522997548 2313 70-71 -10.09656359364985 2313 72-73 -10.070454881961815 2313 74-75 -10.197209938258887 2313 76-77 -10.555403280036872 2313 78-79 -10.7935428159585 2313 80-81 -11.011375742017044 2313 82-83 -11.076900932158908 2313 84-85 -11.257657987773142 2313 86-87 -11.50224123441049 2313 88-89 -11.52743573171459 2313 90-91 -11.549351902594347 2313 92-93 -11.332214988082043 2313 94-95 -11.247233815876914 2313 96-97 -10.975272141240474 2313 98-99 -10.639228197074512 2313 100-101 -9.582479579606787 2314 1 -23.79017246112885 2314 2 -22.681953744955663 2314 3 -23.471185847462344 2314 4 -25.874038317243226 2314 5 -25.88305010015287 2314 6 -25.60534424391527 2314 7 -26.15741301395186 2314 8 -26.339343386526785 2314 9 -27.67341045830407 2314 10-11 -27.040908506664703 2314 12-13 -27.330946132517674 2314 14-15 -28.151107657399237 2314 16-17 -28.98959732182501 2314 18-19 -28.10208491928487 2314 20-21 -27.42431899959106 2314 22-23 -27.281133984675375 2314 24-25 -27.919404356270505 2314 26-27 -27.749586475519862 2314 28-29 -28.41575307213722 2314 30-31 -28.216793022803696 2314 32-33 -27.928458423987962 2314 34-35 -28.08518457271316 2314 36-37 -28.013131652996282 2314 38-39 -27.242619320736893 2314 40-41 -27.032156470791083 2314 42-43 -27.284290419627233 2314 44-45 -27.432880870478122 2314 46-47 -26.796209497860065 2314 48-49 -27.441761000329933 2314 50-51 -26.893952348876972 2314 52-53 -26.57904786417649 2314 54-55 -26.314225392450464 2314 56-57 -26.213231329675185 2314 58-59 -26.121306594220332 2314 60-61 -25.88115205595848 2314 62-63 -25.686986556670245 2314 64-65 -25.44329619742063 2314 66-67 -25.13883506581505 2314 68-69 -24.75927145518176 2314 70-71 -24.59764952111727 2314 72-73 -24.191325489648513 2314 74-75 -23.765122347347244 2314 76-77 -23.402993813701784 2314 78-79 -23.054080375986665 2314 80-81 -22.579252488120893 2314 82-83 -22.388919198091717 2314 84-85 -22.140561106966803 2314 86-87 -21.78159882824732 2314 88-89 -21.422508991546803 2314 90-91 -21.124224643770358 2314 92-93 -20.889765552819796 2314 94-95 -20.601748419688754 2314 96-97 -20.262613266734398 2314 98-99 -19.865601760823637 2314 100-101 -18.43587818649648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6505061.0 3 342319.0 4 310049.0 5 307436.0 6 353093.0 7 351126.0 8 365623.0 9 405222.0 10 442054.0 11 495751.0 12 547144.0 13 601586.0 14 654820.0 15 727278.0 16 790324.0 17 824425.0 18 823323.0 19 847921.0 20 862692.0 21 825609.0 22 890093.0 23 996864.0 24 1200403.0 25 1485516.0 26 1825012.0 27 2116985.0 28 2257117.0 29 2417343.0 30 2797573.0 31 3229738.0 32 3798646.0 33 4618656.0 34 5693655.0 35 7124794.0 36 9261156.0 37 1.2996275E7 38 1.3409496E7 39 3085565.0 40 4759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.46404476343593 16.345476149348983 15.620277565737572 32.57020152147752 2 21.468384726485333 19.681117441669603 42.831896303575945 16.01860152826912 3 23.25481819317911 22.835052422722825 32.69962639331375 21.210502990784313 4 22.072432886581893 31.585669197163902 29.11330566018236 17.22859225607185 5 21.50915936043293 34.19290260105148 29.86618908388682 14.431748954628773 6 17.879026253062545 36.80248182465461 29.77485984845934 15.543632073823499 7 16.07951742188143 19.21645153411187 47.407641515705265 17.296389528301436 8 17.163032107734796 25.389370497396158 37.97247467941941 19.475122715449633 9 20.169044608213323 22.41453444920938 37.19100367920515 20.225417263372147 10-11 20.82475594908968 34.57080266334598 27.60489472573778 16.999546661826567 12-13 19.147665077442596 30.350997580263435 33.34573966447847 17.1555976778155 14-15 19.540850431295063 29.21433983005246 34.41735607466482 16.827453663987654 16-17 19.983723172124833 28.834517087368074 32.67408457661073 18.507675163896366 18-19 20.43831667250148 29.64885770214336 29.730630002878378 20.182195622476783 20-21 20.41797776908533 30.305450380297543 29.657125248065245 19.619446602551882 22-23 20.18906485926588 29.862227767259274 32.477406846960065 17.47130052651478 24-25 19.584523844582616 29.82063855127005 32.55559442254871 18.039243181598618 26-27 20.21030641476827 30.255370098193236 30.671885564353534 18.862437922684965 28-29 20.749865759693765 30.042530112966716 29.67091460031582 19.536689527023697 30-31 20.28222137899188 29.00970371228173 31.476421408771394 19.231653499954994 32-33 21.033269431814006 29.4541240104971 31.20414144423008 18.30846511345882 34-35 18.552122074477715 32.349688660086386 29.667130647330335 19.43105861810556 36-37 19.2231366273119 30.554626755359205 30.5218100956735 19.700426521655395 38-39 20.034688596760137 28.806766677046554 31.944590520929488 19.21395420526382 40-41 20.667392554994667 29.562757274063227 30.3666754636373 19.403174707304803 42-43 20.230133211864988 30.802024197287324 30.712506351144047 18.25533623970364 44-45 19.952938044914113 30.36894610559486 30.847155633608153 18.83096021588288 46-47 19.808792762565155 29.825477416408336 30.90602077517585 19.459709045850655 48-49 20.652812754809126 29.542077406088126 29.973387567170207 19.831722271932538 50-51 20.216493374991398 30.06535901354908 30.326085589227127 19.3920620222324 52-53 19.399060667236554 30.203559844791116 31.609393018708293 18.787986469264037 54-55 19.231924698655064 30.153251188859798 31.702113452417198 18.91271066006794 56-57 19.807432296824473 29.25777059944562 30.53312845628578 20.401668647444122 58-59 20.446107461427104 29.11074894324688 30.279752887363394 20.163390707962623 60-61 21.12530487367115 29.098426473944556 30.60987427866335 19.166394373720944 62-63 18.856189789969413 31.312480134327608 30.857444814919482 18.973885260783494 64-65 18.295658615491732 31.625765649508637 30.5441109764665 19.534464758533133 66-67 19.03892909625064 29.943147885910886 31.31047166681648 19.707451351021994 68-69 20.36759022972611 30.38636011312762 30.143050337385404 19.102999319760865 70-71 19.403821323522607 31.991683474562034 29.50923250750871 19.095262694406653 72-73 18.69679945774392 31.594690581822686 30.352286923426792 19.356223037006597 74-75 19.051821802435068 29.90737960789981 31.408833989904426 19.631964599760693 76-77 19.469578194191637 30.137073647686325 29.98078341732316 20.41256474079888 78-79 19.52978631286278 31.171887170420437 29.197448233892576 20.10087828282421 80-81 19.504998617519874 31.352191633318217 30.601499977601353 18.54130977156056 82-83 19.606969957588852 31.035690816523186 30.455376213523135 18.90196301236483 84-85 19.206506192884483 31.22993169718699 29.927349127556486 19.636212982372037 86-87 20.016208283768353 31.7791072295526 28.65915293246789 19.545531554211156 88-89 19.642351955864918 30.800420290713166 30.342803497981556 19.214424255440363 90-91 19.87247214260151 31.460247966982006 29.648188575381624 19.01909131503486 92-93 18.830229254193924 32.2210784169322 29.46069114668754 19.488001182186334 94-95 18.742430657146684 32.39389571311166 29.46958945503094 19.394084174710713 96-97 19.445759405374826 30.37064193656328 30.791418322142093 19.3921803359198 98-99 19.78922716930911 31.042344385818392 29.247971101120473 19.920457343752023 100-101 20.41946204021625 32.02728823266851 28.309168189832846 19.244081537282394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4808.0 1 4736.5 2 5481.0 3 7536.0 4 10171.5 5 12913.0 6 15897.0 7 18581.0 8 21766.0 9 27090.0 10 34989.0 11 48486.5 12 62547.5 13 84069.0 14 103732.0 15 155064.0 16 353083.5 17 496515.0 18 590650.0 19 683438.5 20 727932.5 21 667720.5 22 520724.5 23 551009.5 24 738461.0 25 944806.0 26 1126815.5 27 1198269.5 28 1305319.0 29 1499165.0 30 1727056.0 31 2101777.5 32 2601772.0 33 3314875.5 34 4186378.5 35 5125733.0 36 5950920.0 37 6331818.0 38 5855383.5 39 4872561.0 40 4144773.5 41 3706763.0 42 3361365.0 43 3098082.5 44 2881889.0 45 2712223.0 46 2603444.0 47 2458000.0 48 2321376.5 49 2193009.0 50 2010854.0 51 1875408.0 52 1733978.0 53 1461193.0 54 1202324.5 55 1020384.5 56 864586.5 57 714737.0 58 568703.5 59 432911.5 60 317344.0 61 227359.0 62 161193.5 63 114829.0 64 82072.5 65 59475.5 66 43145.0 67 31864.5 68 23996.0 69 17858.0 70 13477.0 71 10225.0 72 7943.5 73 6295.5 74 5057.5 75 3888.0 76 2952.5 77 2318.5 78 1845.5 79 1478.0 80 1182.5 81 907.5 82 632.5 83 437.5 84 319.0 85 211.0 86 138.5 87 102.5 88 80.5 89 54.5 90 38.5 91 25.0 92 21.0 93 23.0 94 16.0 95 10.0 96 9.0 97 7.0 98 3.5 99 2.5 100 4.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 4.218413350551785 2 9.384636294026217 3 4.880913013310288 4 3.234254145316579 5 1.928964424174456 6 2.225566121063931 7 7.710027016382701 8 6.394518075533441 9 5.161923437908255 10-11 1.6649553192027267 12-13 3.070191203867977 14-15 3.9347681458753394 16-17 3.4091631667228164 18-19 1.911620945484982 20-21 1.7749716225385692 22-23 2.083630673527848 24-25 2.2355078865231177 26-27 1.6207660714700194 28-29 1.4535957459720839 30-31 1.459326004413883 32-33 1.9280585567604407 34-35 1.9461784932333568 36-37 1.8389952255300313 38-39 1.2021026228309108 40-41 1.7148448023429397 42-43 2.743257960126139 44-45 1.4437839077819932 46-47 0.633679620391239 48-49 0.7446416493073137 50-51 0.7446416493073137 52-53 0.8041240095426869 54-55 0.3307508278437596 56-57 0.2377679377225367 58-59 0.9281734932179311 60-61 0.8245686606192268 62-63 0.0 64-65 0.03625695501706747 66-67 0.27183786998291026 68-69 0.0 70-71 0.0 72-73 0.2372596166936436 74-75 0.3307508278437596 76-77 0.2355809149658428 78-79 0.2944938728266921 80-81 0.05969459202951385 82-83 0.3632021044449185 84-85 0.0690348615257942 86-87 0.034407432576909536 88-89 0.07126536591836083 90-91 0.10492429319203264 92-93 0.06860108044411149 94-95 0.06854206965256993 96-97 0.10504386769068265 98-99 0.0690358968028388 100-101 0.06922535250199854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9.6592502E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.42381725908104 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22628550660379 43.10615765291221 2 9.606099355927993 10.071767944154903 3 3.2874063789426256 5.170151737980766 4 1.5593298319177866 3.269840886203664 5 0.8659984160192881 2.26994713540244 6 0.5497440826380906 1.7291809996488248 7 0.3678026905143669 1.3497134724446458 8 0.26156776939673587 1.0969904754975945 9 0.1879291356206621 0.8866766397091183 >10 0.9682333760106231 9.092439205099803 >50 0.06779647179906255 2.456103281273073 >100 0.043127833538278225 4.416757807896431 >500 0.004410518531844855 1.5986581639136397 >1k 0.0032796606743698145 3.3663764709753727 >5k 4.8747528260543695E-4 1.7882719647862242 >10k+ 5.014965816752058E-4 8.330966162101516 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 291282 0.30155756810192164 No Hit GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG 254356 0.2633289279534347 No Hit GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA 188541 0.19519216926382132 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 178049 0.1843300425119954 No Hit GTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA 150971 0.15629681069861923 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 145378 0.15050650618823394 No Hit ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG 137066 0.14190128339361163 No Hit CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA 134506 0.13925097415946425 No Hit CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG 130978 0.1355985167461549 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 125898 0.13033930935964366 No Hit CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC 122980 0.1273183709435335 No Hit GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT 122113 0.12642078574587495 No Hit CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT 101891 0.10548541335019979 No Hit GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG 99191 0.10269016532980996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006502575117062399 2.0705540891776465E-6 0.0 1.2423324535065879E-5 0.0 2 0.006510857333419109 1.1388047490477056E-5 0.0 2.8987757248487052E-5 0.0 3 0.006529492320221708 1.2423324535065879E-5 0.0 3.10583113376647E-5 0.0 4 0.006613349760833403 1.552915566883235E-5 0.0 4.3481635872730575E-5 0.0 5 0.006633020024680591 1.552915566883235E-5 0.0 5.4869683363207634E-5 0.0 6 0.006682713322820854 1.656443271342117E-5 0.0 6.21166226753294E-5 0.0 7 0.006711701080069341 2.0705540891776466E-5 0.0 6.522245380909587E-5 1.0352770445888232E-6 8 0.006809017122260691 2.3811372025542936E-5 0.0 7.350467016580645E-5 1.0352770445888232E-6 9 0.006948779523280182 2.4846649070131757E-5 0.0 8.075160947792821E-5 1.0352770445888232E-6 10-11 0.00721018697703886 2.7434841681603817E-5 0.0 8.748091026775556E-5 1.0352770445888232E-6 12-13 0.007582369074568542 2.8470118726192638E-5 0.0 1.0042187332511586E-4 1.0352770445888232E-6 14-15 0.008087584272327888 3.209358838225352E-5 0.0 1.066335355926488E-4 1.0352770445888232E-6 16-17 0.008793643216737465 3.312886542684234E-5 0.0 1.247508838729532E-4 1.5529155668832348E-6 18-19 0.009696404799618918 3.5199419516019985E-5 0.0 1.3303310022966377E-4 3.1058311337664697E-6 20-21 0.010612624984080027 3.8305250649786456E-5 0.0 1.4338587067555203E-4 3.1058311337664697E-6 22-23 0.01162564357221019 4.2446358828141754E-5 0.0 1.5684447225520673E-4 3.1058311337664697E-6 24-25 0.012661438255321308 4.5552189961908224E-5 0.0 1.7237362792403907E-4 3.1058311337664697E-6 26-27 0.013783678571655593 5.072857518485234E-5 0.0 1.868675065482826E-4 4.141108178355293E-6 28-29 0.015001682014614343 5.3834406318618806E-5 0.0 2.0291430073940934E-4 4.141108178355293E-6 30-31 0.016292672489216606 5.9010791541562925E-5 0.0 2.1274943266300317E-4 4.141108178355293E-6 32-33 0.017665449850341387 6.0046068586151746E-5 0.0 2.277609498095411E-4 4.658746700649705E-6 34-35 0.019093614533351667 6.108134563074057E-5 0.0 2.4277246695607907E-4 5.1763852229441166E-6 36-37 0.020571990153024508 6.315189971991822E-5 0.0 2.7072494715997726E-4 5.1763852229441166E-6 38-39 0.022133187936264453 6.574009233139029E-5 0.0 3.0074798145305313E-4 5.1763852229441166E-6 40-41 0.023833630482001593 7.609286277727851E-5 0.0 3.2197116086712404E-4 5.1763852229441166E-6 42-43 0.025694023331127712 7.868105538875057E-5 0.0 3.4474725584807814E-4 5.1763852229441166E-6 44-45 0.027634132512687166 8.02339709556338E-5 0.0 3.789113983195093E-4 5.1763852229441166E-6 46-47 0.029692780915852042 8.230452504481145E-5 0.0 4.317105275935393E-4 5.1763852229441166E-6 48-49 0.03207288284136174 8.696327174546115E-5 0.0 4.498278758738437E-4 5.1763852229441166E-6 50-51 0.03484846059790438 9.36925725352885E-5 0.0 4.865802109567469E-4 5.694023745238528E-6 52-53 0.037804176560205474 1.0870408968182644E-4 0.0 5.140150526383508E-4 6.729300789827351E-6 54-55 0.04082407969927107 1.1698630603853702E-4 0.0 5.300618468294775E-4 7.764577834416175E-6 56-57 0.04421305910473258 1.1750394456083144E-4 0.0 5.673318204346752E-4 8.282216356710586E-6 58-59 0.04836969643875671 1.2319796830606998E-4 0.0 6.211662267532939E-4 8.282216356710586E-6 60-61 0.052959597215941255 1.247508838729532E-4 0.0 6.522245380909586E-4 8.282216356710586E-6 62-63 0.058411366132745994 1.2785671500671966E-4 0.0 7.412583639255974E-4 8.282216356710586E-6 64-65 0.06491497652685299 1.2889199205130852E-4 0.0 7.899163850212721E-4 9.835131923593821E-6 66-67 0.07328363851678674 1.3148018466278057E-4 0.0 8.256334430595865E-4 1.0352770445888233E-5 68-69 0.08402619076996265 1.3251546170736937E-4 0.0 8.541035617857792E-4 1.0352770445888233E-5 70-71 0.09801433655792455 1.3613893136343026E-4 0.0 8.706679944992003E-4 1.2940963057360291E-5 72-73 0.11606542710737527 1.387271239749023E-4 0.0 8.794678493782053E-4 1.3458601579654703E-5 74-75 0.14031938006947992 1.3924476249719674E-4 1.0352770445888232E-6 8.90338258346388E-4 1.3458601579654703E-5 76-77 0.17281983233025686 1.4079767806407997E-4 1.0352770445888232E-6 9.012086673145707E-4 1.3458601579654703E-5 78-79 0.21710329027402148 1.418329551086688E-4 1.0352770445888232E-6 9.146672688942253E-4 1.4493878624243526E-5 80-81 0.2762113978577757 1.4390350919784643E-4 1.0352770445888232E-6 9.234671237732304E-4 1.4493878624243526E-5 82-83 0.3518777264926837 1.4442114772014085E-4 1.0352770445888232E-6 9.348551712637074E-4 1.4493878624243526E-5 84-85 0.4571291672308064 1.464917018093185E-4 1.0352770445888232E-6 9.524548810217173E-4 1.4493878624243526E-5 86-87 0.6002960768114278 1.470093403316129E-4 1.0352770445888232E-6 9.617723744230167E-4 1.4493878624243526E-5 88-89 0.7785930423460818 1.470093403316129E-4 1.0352770445888232E-6 9.716075063466107E-4 1.4493878624243526E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCAG 35880 0.0 40.90053 1 TCACGGT 58645 0.0 28.487751 2 CGGCGAG 7705 0.0 28.483414 1 CACGGTC 66085 0.0 25.059639 3 GCGACGG 2890 0.0 24.314686 1 CGACGGC 12410 0.0 23.9623 1 GACGGCC 17675 0.0 23.421467 2 TTGGCGA 12050 0.0 22.734354 1 TTACGCG 12205 0.0 21.376852 8 GGCCAGT 68910 0.0 21.170216 2 ACGGCCA 20210 0.0 20.417503 3 TACGCGC 13155 0.0 19.977356 9 CTGGAAT 286755 0.0 19.667961 1 CGGACAG 11345 0.0 18.895788 1 CGACGGA 2540 0.0 18.443422 1 GCCAGTG 79815 0.0 18.42454 3 ACGGTCT 94195 0.0 17.447317 4 CTTTTAT 238630 0.0 16.838152 1 TGAATTG 132475 0.0 16.420267 2 TATTATA 216005 0.0 16.338179 9 >>END_MODULE