FastQCFastQC Report
Fri 27 May 2016
SRR1233116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96592502
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC3921270.4059600816634815No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG2646050.2739394823834256No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT2289030.23697802133751542No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1951910.202076761610337No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1798970.18624323449039554No Hit
GTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA1791870.18550818778873748No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1741430.18028625037583146No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1715180.17756864813378578No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1536950.15911690536807918No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1433020.14835727104366755No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1330020.13769391748440266No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1228250.12715790300162222No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1192650.12347231672288601No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA1191180.12332013099733145No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC1142420.11827212012791635No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1133850.11738488770070372No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT1067050.11046923704285039No Hit
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA1051770.10888733371871866No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA999520.10347801116074207No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG973630.1007976788923016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG310300.048.780091
TCACGGT673950.027.3187712
CACGGTC750400.024.6429563
CTGGAAT2776400.022.7071231
GGCCAGT667050.022.6895522
CGGTCAG69450.022.3623711
TTACGCG153850.022.3428158
TACGCGC163450.021.1909489
CGGACAG83250.020.0817151
GCCAGTG794000.019.3782793
ATTACGC190350.018.307627
TGAATTG1421100.017.9372582
GCGCGTA58250.017.5869980-81
TATTATA2617100.017.56239
GATTACG203050.017.5128026
CGCGCAG171900.017.50005110-11
GTGAATT1656100.017.201341
CTTTTAT2755950.017.1300011
AGTGAAT1138900.017.0726766
ACGGTCT1113250.016.7215334
Produced by FastQC (version 0.11.5)