FastQCFastQC Report
Fri 27 May 2016
SRR1233115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31245237
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC519850.16637735857148403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461070.14756489125046482No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG409300.13099596588113574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGA12000.030.2566946-47
GAGCGGC27350.026.2364489
CGGCGAG17550.025.7847961
GGCGCCG16650.025.37172544-45
GATCGGG26000.023.8170071
TCGGGGG38050.022.08327138-39
CGGCGCT12450.021.0431391
GCGCCGG16000.020.3209944-45
CTGGAAT900900.019.6373331
GCCGGAT23400.019.57278846-47
GGGCGCC25450.019.3968242-43
AGCGGCG20700.019.27376410-11
ATCGGGA32250.018.9059052
CCGGATC21850.018.57176248-49
CTCGGGG39300.017.33522836-37
AGAGCGG45650.016.880498
CGACGCT5200.016.4886991
GCGGCGT14050.015.8883610-11
GGGGCGC28050.015.65286742-43
CGGCGGG13750.015.02033612-13
Produced by FastQC (version 0.11.5)