FastQCFastQC Report
Fri 27 May 2016
SRR1233115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31245237
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT664240.2125892019958114No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC535050.17124210003591908No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG394390.1262240385630616No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT353910.11326846392619777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGC51350.035.815211
CGGCGCT17450.034.0417251
GACGGCC66300.029.4433122
CGGCGAG17600.026.1912381
CCGACGG7600.025.6370011
ACGGCCA80100.024.5476673
TATGCCG65450.023.46241846-47
CGGCCAG106000.023.2679671
ATGCCGT66050.021.99159446-47
TGCCGTC68850.021.86683348-49
CGTATGC74900.020.34374844-45
GCCGTCT71400.020.18789748-49
CTCGTAT70450.019.8768942-43
CTGGAAT905900.019.8713191
CCGTCTT76000.019.58771950-51
TCTCGTA68300.019.56433740-41
TCGTATG79100.018.7534442-43
CGGCGAT4600.018.5956611
CGTCTTC81600.017.68886450-51
GCGACGG8500.017.3316441
Produced by FastQC (version 0.11.5)