##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31245237 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35014523973686 33.0 31.0 34.0 30.0 34.0 2 32.59714752683745 34.0 31.0 34.0 31.0 34.0 3 32.672126314804395 34.0 31.0 34.0 31.0 34.0 4 36.06983502797562 37.0 35.0 37.0 35.0 37.0 5 36.058715765222075 37.0 35.0 37.0 35.0 37.0 6 36.052909792298905 37.0 35.0 37.0 35.0 37.0 7 36.03744013847614 37.0 35.0 37.0 35.0 37.0 8 36.04054880428655 37.0 35.0 37.0 35.0 37.0 9 37.819305387249905 39.0 38.0 39.0 35.0 39.0 10-11 37.83586272685338 39.0 38.0 39.0 35.0 39.0 12-13 37.74225165902887 39.0 37.5 39.0 35.0 39.0 14-15 39.081326875517064 40.0 38.0 41.0 36.0 41.0 16-17 38.96792528730059 40.0 38.0 41.0 36.0 41.0 18-19 38.994526461745195 40.0 38.5 41.0 35.5 41.0 20-21 39.00409395198379 40.0 39.0 41.0 35.0 41.0 22-23 38.996078394284545 40.0 39.0 41.0 35.0 41.0 24-25 38.42143512945669 40.0 38.0 41.0 33.5 41.0 26-27 37.432619074068796 40.0 37.0 41.0 31.0 41.0 28-29 36.97548954421437 40.0 36.0 41.0 30.5 41.0 30-31 36.90324152446019 39.0 36.0 41.0 30.0 41.0 32-33 36.91973069367341 39.0 36.0 41.0 30.0 41.0 34-35 36.815130638951466 39.0 36.0 41.0 30.0 41.0 36-37 36.69022873790331 39.0 36.0 41.0 30.0 41.0 38-39 36.570936011783175 39.0 35.0 41.0 30.0 41.0 40-41 36.41709389178261 39.0 35.0 41.0 30.0 41.0 42-43 36.297670009672196 39.0 35.0 41.0 29.0 41.0 44-45 36.14991478541194 39.0 35.0 41.0 28.5 41.0 46-47 35.98003422089582 39.0 35.0 41.0 27.5 41.0 48-49 36.23311485843426 39.0 35.0 41.0 29.0 41.0 50-51 36.20866356366572 39.0 35.0 41.0 28.0 41.0 52-53 36.11610057558533 39.0 35.0 41.0 28.0 41.0 54-55 35.98752566671202 39.0 35.0 41.0 28.0 41.0 56-57 35.665916888388466 39.0 35.0 41.0 26.5 41.0 58-59 35.52774546085216 39.0 34.5 41.0 26.0 41.0 60-61 35.2523529426261 39.0 34.0 41.0 26.0 41.0 62-63 35.12563436468733 38.0 34.0 41.0 26.0 41.0 64-65 34.975363268968 38.0 34.0 40.5 26.0 41.0 66-67 34.71344896503746 38.0 34.0 40.0 26.0 41.0 68-69 34.38140435612634 37.0 34.0 40.0 25.5 41.0 70-71 34.0758588100964 37.0 33.0 39.5 25.0 41.0 72-73 33.75522165186329 36.5 33.0 39.0 24.5 41.0 74-75 33.37394848373209 36.0 32.5 39.0 23.5 41.0 76-77 32.986705989780134 35.5 32.0 39.0 22.5 40.0 78-79 32.611928547701524 35.0 32.0 37.5 22.0 39.0 80-81 32.24041752347726 35.0 32.0 37.0 22.0 39.0 82-83 31.899776516337514 35.0 32.0 36.5 22.0 39.0 84-85 31.55510003012619 35.0 31.0 36.0 21.0 37.5 86-87 31.204484206664844 35.0 31.0 36.0 20.0 37.0 88-89 30.88129627565315 35.0 31.0 35.5 19.5 37.0 90-91 30.6078477817275 35.0 31.0 35.0 18.5 36.0 92-93 30.356206499569836 35.0 31.0 35.0 16.5 36.0 94-95 30.12014584814959 34.0 31.0 35.0 9.0 36.0 96-97 29.828442123834748 34.0 31.0 35.0 2.0 36.0 98-99 29.500823629534317 34.0 31.0 35.0 2.0 35.0 100-101 28.554303956791877 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26193702524366813 1101 2 0.24645513358960613 1101 3 0.2015995507044721 1101 4 0.2477229434407846 1101 5 0.26285798297582375 1101 6 0.19605694616909375 1101 7 0.20101134965432976 1101 8 0.21848709289044876 1101 9 0.23927915582623172 1101 10-11 0.23281856461658634 1101 12-13 0.26835640445165865 1101 14-15 0.31682181288848454 1101 16-17 0.25297881250639875 1101 18-19 0.2420502102145079 1101 20-21 0.2792452571644475 1101 22-23 0.3286727056315044 1101 24-25 0.34576417974044915 1101 26-27 0.4042277067007234 1101 28-29 0.6203676294312075 1101 30-31 0.48149619753279893 1101 32-33 0.38654836690493966 1101 34-35 0.3270908997102495 1101 36-37 0.41741851589242174 1101 38-39 0.4551771401526139 1101 40-41 0.33073711229904035 1101 42-43 0.21435909321167657 1101 44-45 0.22132146411993858 1101 46-47 0.3674116527029625 1101 48-49 0.39262273951280235 1101 50-51 0.4178452727427526 1101 52-53 0.27795075411711423 1101 54-55 0.44080255239024524 1101 56-57 0.558314083436052 1101 58-59 0.5270128010550721 1101 60-61 0.5852355489792842 1101 62-63 0.5141585401468802 1101 64-65 0.32949118192352245 1101 66-67 0.39901261419211664 1101 68-69 0.3257812783880709 1101 70-71 0.3710375999479041 1101 72-73 0.3056890845586153 1101 74-75 0.31887344092043435 1101 76-77 0.36942946541471144 1101 78-79 0.34983363257362754 1101 80-81 0.38284255149024915 1101 82-83 0.33170962702431694 1101 84-85 0.29558551771984654 1101 86-87 0.3149628327476002 1101 88-89 0.31048994786621975 1101 90-91 0.335342285633228 1101 92-93 0.3277756015331441 1101 94-95 0.3625897469618451 1101 96-97 0.3919346202935685 1101 98-99 0.29243428211003675 1101 100-101 0.3128736433706436 1102 1 0.23752040261766894 1102 2 0.23251006146251996 1102 3 0.2055411026442755 1102 4 0.2578553256338125 1102 5 0.2629273838795356 1102 6 0.1904672817556161 1102 7 0.18058682481444066 1102 8 0.17796488426693458 1102 9 0.22270634749619234 1102 10-11 0.23732601683278887 1102 12-13 0.26114275604805215 1102 14-15 0.28436672858587997 1102 16-17 0.3297628295900168 1102 18-19 0.36367249257694567 1102 20-21 0.37524630202881326 1102 22-23 0.35441991678770535 1102 24-25 0.3947839572773191 1102 26-27 0.38134747388840395 1102 28-29 0.4677060992531423 1102 30-31 0.24812373009848443 1102 32-33 0.23186252870517166 1102 34-35 0.35293927008810755 1102 36-37 0.40478452114014374 1102 38-39 0.3746457865608761 1102 40-41 0.2621212200055041 1102 42-43 0.30589428943881103 1102 44-45 0.35782101680824496 1102 46-47 0.38160103188636185 1102 48-49 0.33796494392223053 1102 50-51 0.39490490638126374 1102 52-53 0.3494538848899751 1102 54-55 0.4086879047548777 1102 56-57 0.5105313103080604 1102 58-59 0.4297699240473847 1102 60-61 0.539793341113743 1102 62-63 0.4296424032309645 1102 64-65 0.46267259874004907 1102 66-67 0.35450235478636216 1102 68-69 0.3877584359049422 1102 70-71 0.3925781868078815 1102 72-73 0.42949313135293465 1102 74-75 0.4338024965941827 1102 76-77 0.4421294193924581 1102 78-79 0.421550984435747 1102 80-81 0.3810377848988509 1102 82-83 0.39577936040029726 1102 84-85 0.3656695890931694 1102 86-87 0.42228573679745196 1102 88-89 0.44849412115083 1102 90-91 0.3918535808028025 1102 92-93 0.33601955836154573 1102 94-95 0.290243051403138 1102 96-97 0.36870083359301375 1102 98-99 0.4068524677562273 1102 100-101 0.4231105707627698 1103 1 0.19718452738803194 1103 2 0.19322923758992516 1103 3 0.15535570348501437 1103 4 0.2469098593276371 1103 5 0.22986891359375505 1103 6 0.17844814962505495 1103 7 0.18343284098791202 1103 8 0.1909811507770769 1103 9 0.16687163407593886 1103 10-11 0.16951132504474486 1103 12-13 0.24234159370859487 1103 14-15 0.2662917554650406 1103 16-17 0.31728704100367366 1103 18-19 0.33877588058887653 1103 20-21 0.3557095924852405 1103 22-23 0.3340917525549756 1103 24-25 0.33700269656745263 1103 26-27 0.36453823169043886 1103 28-29 0.5893486777777852 1103 30-31 0.5255824454177329 1103 32-33 0.35438123096619023 1103 34-35 0.3710629867317934 1103 36-37 0.374416715861436 1103 38-39 0.38258031517911206 1103 40-41 0.3745485024098869 1103 42-43 0.2553367742372856 1103 44-45 0.27443572512277825 1103 46-47 0.30711436234374645 1103 48-49 0.31991442991679975 1103 50-51 0.3199651065837017 1103 52-53 0.39664508862345116 1103 54-55 0.3908845358673645 1103 56-57 0.5562250777881061 1103 58-59 0.5107599606152675 1103 60-61 0.547540337217697 1103 62-63 0.4560687670671655 1103 64-65 0.3940855945961843 1103 66-67 0.38365951617801386 1103 68-69 0.31786142488107316 1103 70-71 0.35724534130908836 1103 72-73 0.36630514926992674 1103 74-75 0.3622095892519255 1103 76-77 0.377827068738533 1103 78-79 0.3371031551451438 1103 80-81 0.35073901943763985 1103 82-83 0.42839942127298514 1103 84-85 0.44500809744831216 1103 86-87 0.43406415175356017 1103 88-89 0.4378915941886383 1103 90-91 0.4237354516333056 1103 92-93 0.4145324038801341 1103 94-95 0.46461170555019393 1103 96-97 0.48095517335248417 1103 98-99 0.45300361671842637 1103 100-101 0.4807705004145575 1104 1 0.17040116214404577 1104 2 0.13768847746657542 1104 3 0.135028377585364 1104 4 0.20649114064622864 1104 5 0.23834863386465344 1104 6 0.1714303757192539 1104 7 0.15250768183933872 1104 8 0.1618394039889921 1104 9 0.15934460023721186 1104 10-11 0.12923858258383802 1104 12-13 0.14401216790339078 1104 14-15 0.23291235187690518 1104 16-17 0.27613108702858113 1104 18-19 0.2218116575400515 1104 20-21 0.227613697148783 1104 22-23 0.2264560826846207 1104 24-25 0.25982606398741837 1104 26-27 0.24729708417159202 1104 28-29 0.449087601580608 1104 30-31 0.3357585657501332 1104 32-33 0.2750288590107104 1104 34-35 0.27057190353974647 1104 36-37 0.25079616818044315 1104 38-39 0.2132395106522864 1104 40-41 0.15475270976256894 1104 42-43 0.20186404116022771 1104 44-45 0.15143590715315725 1104 46-47 0.21204153291382255 1104 48-49 0.2389164905732173 1104 50-51 0.2022647905423085 1104 52-53 0.23233910727842755 1104 54-55 0.2445498072209844 1104 56-57 0.3119033096043111 1104 58-59 0.31793890380290435 1104 60-61 0.4470879388609106 1104 62-63 0.31669720741148666 1104 64-65 0.2917180476151131 1104 66-67 0.30996553049727993 1104 68-69 0.29053965778570046 1104 70-71 0.22061936675000737 1104 72-73 0.23703374664878396 1104 74-75 0.19644921113859937 1104 76-77 0.21368725248498066 1104 78-79 0.22203531082319472 1104 80-81 0.2143590331681935 1104 82-83 0.24415594459943435 1104 84-85 0.2267804550368382 1104 86-87 0.16142838688319827 1104 88-89 0.13394631460855067 1104 90-91 0.1712536960904636 1104 92-93 0.22825733326529019 1104 94-95 0.2597239267454441 1104 96-97 0.18806717130320294 1104 98-99 0.2564302642004215 1104 100-101 0.22961337130290715 1105 1 0.11232109278336111 1105 2 0.0928497769599872 1105 3 0.07317576033085516 1105 4 0.1566463533208875 1105 5 0.18411582496214152 1105 6 0.054080619502244076 1105 7 0.08049573644082386 1105 8 0.08270700258962904 1105 9 0.0841824540076388 1105 10-11 0.08545387803538773 1105 12-13 0.09183520015381674 1105 14-15 0.14614837514070445 1105 16-17 0.19305546062569334 1105 18-19 0.1479060359394353 1105 20-21 0.18936851183070758 1105 22-23 0.15392442154553976 1105 24-25 0.1689579456041841 1105 26-27 0.15936994777015911 1105 28-29 0.39916517649295 1105 30-31 0.30238441136301475 1105 32-33 0.18175689920538218 1105 34-35 0.14862316247540974 1105 36-37 0.12183887882518718 1105 38-39 0.06075960806599312 1105 40-41 0.14865855896029956 1105 42-43 0.10317077622691073 1105 44-45 0.11575028207467142 1105 46-47 0.1563182015991771 1105 48-49 0.12506792484156648 1105 50-51 0.18794337308138154 1105 52-53 0.18331322627700786 1105 54-55 0.203860561182033 1105 56-57 0.29262335199354084 1105 58-59 0.2452578061686168 1105 60-61 0.29037356051073715 1105 62-63 0.18625269794184618 1105 64-65 0.20571728945729717 1105 66-67 0.16892153383523123 1105 68-69 0.133479862032587 1105 70-71 0.1245079109447218 1105 72-73 0.092135080587191 1105 74-75 0.06646351779701831 1105 76-77 0.19775616846371946 1105 78-79 0.10957765699161115 1105 80-81 0.1175080899961145 1105 82-83 0.04836923323814801 1105 84-85 0.10037749800738638 1105 86-87 0.16248660849871044 1105 88-89 0.14082552100976997 1105 90-91 0.06905371856668197 1105 92-93 0.0406060848188865 1105 94-95 0.060720816822083634 1105 96-97 0.11295629526879836 1105 98-99 0.10086715216209186 1105 100-101 0.06061820093509951 1106 1 0.06820660116739674 1106 2 0.06709791808028598 1106 3 0.035374540048891845 1106 4 0.13938648265360598 1106 5 0.15950408623155 1106 6 0.07776320022700389 1106 7 0.06894543567941724 1106 8 0.07508382902428679 1106 9 0.0618139449877404 1106 10-11 0.05175201501641169 1106 12-13 0.047715724382378255 1106 14-15 0.04822486223527278 1106 16-17 0.07774551337787017 1106 18-19 0.031364246252067574 1106 20-21 0.09912039288165886 1106 22-23 0.10858196352050697 1106 24-25 0.07601025032126785 1106 26-27 0.033353058605811725 1106 28-29 0.24365171013945286 1106 30-31 0.15987608348846294 1106 32-33 0.05966144365405057 1106 34-35 0.00855150368106905 1106 36-37 -0.04019233659240484 1106 38-39 -0.059830673014204194 1106 40-41 -0.005979627589447034 1106 42-43 -0.01725837923179796 1106 44-45 -0.04318866739726701 1106 46-47 0.08314702931898665 1106 48-49 0.07672407784397706 1106 50-51 0.07924780256689701 1106 52-53 -0.0342768277863712 1106 54-55 0.06164543434434222 1106 56-57 0.22759140407555378 1106 58-59 0.20677199833524185 1106 60-61 0.20307263175492807 1106 62-63 0.09182291697019451 1106 64-65 0.1111031017354307 1106 66-67 0.141252526127559 1106 68-69 0.11465384529156353 1106 70-71 0.09697487919118686 1106 72-73 0.02501894384322867 1106 74-75 0.08820763207331339 1106 76-77 0.10399442209791943 1106 78-79 0.09488534173001284 1106 80-81 0.11278052454273535 1106 82-83 0.04551148956518247 1106 84-85 0.09672918175957435 1106 86-87 0.10358250872677743 1106 88-89 0.062465404137416414 1106 90-91 0.09618465280587074 1106 92-93 0.1399629876210433 1106 94-95 0.10593997449512571 1106 96-97 0.0940888859885689 1106 98-99 0.10550185371995724 1106 100-101 0.011698936174816765 1107 1 0.03165231144539149 1107 2 0.021429522654287325 1107 3 0.02911797337322497 1107 4 0.13519017099241637 1107 5 0.14205709933649757 1107 6 0.08824956743858792 1107 7 0.05845273553556041 1107 8 0.039125008792844085 1107 9 0.003568339217132177 1107 10-11 0.007706921333834771 1107 12-13 0.017928326095933755 1107 14-15 0.07623557198126463 1107 16-17 0.15013123093243763 1107 18-19 0.09753878921202386 1107 20-21 0.11535864850608846 1107 22-23 0.04476079283375611 1107 24-25 0.057487796508858935 1107 26-27 -0.03195879758068543 1107 28-29 0.19722536604971452 1107 30-31 0.13847084734589288 1107 32-33 -0.02359061212393243 1107 34-35 -0.04813387359381949 1107 36-37 -0.09166810906223333 1107 38-39 -0.12167881820184334 1107 40-41 -0.034738514253426445 1107 42-43 0.011472923912954514 1107 44-45 -0.03517714537102279 1107 46-47 -0.15072916153564364 1107 48-49 -0.11538017031971037 1107 50-51 -0.06277399123825234 1107 52-53 -0.05957171483181156 1107 54-55 -0.014722115125913149 1107 56-57 0.17319458126156206 1107 58-59 0.14658571773387763 1107 60-61 0.11209608557368966 1107 62-63 0.054563276522408444 1107 64-65 -0.01850395126336224 1107 66-67 -0.09975963734862603 1107 68-69 -0.031983390966878744 1107 70-71 -0.04181208972167383 1107 72-73 -0.017538847270486713 1107 74-75 -0.015243640274057668 1107 76-77 -0.04299839402651173 1107 78-79 -0.051246749720881724 1107 80-81 -0.08459433487421819 1107 82-83 -0.040679928918571306 1107 84-85 -0.004452154451055179 1107 86-87 -0.0398204051544937 1107 88-89 -0.015087273809005097 1107 90-91 -0.047980160488567236 1107 92-93 -0.03232370687874564 1107 94-95 -0.03665246922154353 1107 96-97 -0.08873784549318131 1107 98-99 -0.09507177389872012 1107 100-101 -0.11073351134574239 1108 1 -0.01744461314378043 1108 2 -0.01619965211772012 1108 3 -0.04220586438069773 1108 4 0.07568220803398162 1108 5 0.1086970074775877 1108 6 0.07005822878141288 1108 7 0.048942667419339614 1108 8 0.011114766912648122 1108 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-0.6750533162130701 2215 86-87 -0.6237300433233806 2215 88-89 -0.7809852964929682 2215 90-91 -0.6978634834233652 2215 92-93 -0.6739357857415165 2215 94-95 -0.732916148439422 2215 96-97 -0.7566479647621875 2215 98-99 -0.812285430043918 2215 100-101 -0.822831898299782 2216 1 -1.331367736682342 2216 2 -0.9363010303736594 2216 3 -1.2279090774583636 2216 4 -2.310336038316514 2216 5 -2.798058614781631 2216 6 -1.4093022811426579 2216 7 -0.7826878463133511 2216 8 -0.5621432194834739 2216 9 -0.5357280226861434 2216 10-11 -0.4927336757995917 2216 12-13 -0.5117419222714616 2216 14-15 -0.6289841529619338 2216 16-17 -0.6659508959326601 2216 18-19 -0.7911575924496432 2216 20-21 -0.7654740283558539 2216 22-23 -0.7598120476873973 2216 24-25 -0.7942430454025597 2216 26-27 -0.8233209682339862 2216 28-29 -0.5375761193336146 2216 30-31 -0.7132968784244156 2216 32-33 -0.8123752183039059 2216 34-35 -0.7532709044413153 2216 36-37 -0.9249739304046898 2216 38-39 -0.763041320738779 2216 40-41 -0.786353699429462 2216 42-43 -0.7321142428872704 2216 44-45 -0.7381537967562366 2216 46-47 -0.7033279035259241 2216 48-49 -0.7354153795503038 2216 50-51 -0.7670180637727739 2216 52-53 -0.7592462734516872 2216 54-55 -0.6726851285025717 2216 56-57 -0.7754390680025196 2216 58-59 -0.7761629448833745 2216 60-61 -0.7525046630100931 2216 62-63 -0.8472865544614123 2216 64-65 -0.789699511826278 2216 66-67 -0.7512936910113126 2216 68-69 -0.6800304696615811 2216 70-71 -0.7349135466220531 2216 72-73 -0.7547322921038884 2216 74-75 -0.7542795373381068 2216 76-77 -0.8006061166985461 2216 78-79 -0.8044416256186366 2216 80-81 -0.7953263186693071 2216 82-83 -0.8569259120849253 2216 84-85 -0.7780387206749673 2216 86-87 -0.7826866375632626 2216 88-89 -0.7674307624169536 2216 90-91 -0.8294829697499679 2216 92-93 -0.8492857935240217 2216 94-95 -0.8970005353332837 2216 96-97 -0.8969969250033074 2216 98-99 -0.9249750309949825 2216 100-101 -0.9262291758411578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3188.0 3 1387.0 4 1620.0 5 3096.0 6 6954.0 7 17955.0 8 37023.0 9 56300.0 10 64853.0 11 68881.0 12 80424.0 13 94540.0 14 111043.0 15 125339.0 16 137783.0 17 145453.0 18 147112.0 19 150887.0 20 156296.0 21 165189.0 22 180074.0 23 202672.0 24 235364.0 25 281633.0 26 338488.0 27 410833.0 28 493089.0 29 586633.0 30 707518.0 31 852256.0 32 1039448.0 33 1283907.0 34 1622997.0 35 2138474.0 36 3028264.0 37 4998871.0 38 7819574.0 39 3435154.0 40 14665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.85599750889684 19.13896828006414 20.780972624123812 35.2240615869152 2 17.736363765261494 23.28915522632941 46.037939493290644 12.936541515118453 3 19.055483062817064 28.822011065783233 34.582845116931246 17.53966075446846 4 18.764476206312516 36.432970581631295 27.385042961902666 17.417510250153516 5 19.89361130466061 37.45282520980718 26.693524520233275 15.960038965298933 6 16.310620399518815 38.142923991903146 27.944435179032247 17.60202042954579 7 14.432226065559053 17.689303549201817 48.37026061563315 19.50820976960598 8 17.787540303705153 23.60780147741152 35.2204342465586 23.38422397232473 9 19.100517624494255 22.967158162378475 34.55236073261342 23.379963480513847 10-11 21.879720419467453 34.7849401814427 24.410250112681176 18.925089286408678 12-13 18.687677100993024 27.667436159949755 34.20528223229672 19.439604506760503 14-15 19.124639701084682 28.55740700574619 33.59979154582825 18.718161747340883 16-17 20.378294600537533 28.691719357962448 31.474675588934776 19.45531045256524 18-19 20.97851789710103 29.155798854299793 30.113065148113122 19.752618100486053 20-21 20.11555924906699 29.656141567289218 30.53255663336788 19.69574255027591 22-23 19.627410254446467 30.092735533080493 31.98221153798454 18.297642674488497 24-25 19.765544803827932 29.844600409224988 31.622035726845944 18.767819060101136 26-27 19.782051567222968 29.608540310226505 30.941471555582794 19.667936566967732 28-29 19.98976322026015 29.640314893394347 30.16863962446685 20.201282261878653 30-31 19.205172229267596 29.210091081173182 31.740811422054836 19.84392526750438 32-33 19.852582004062665 29.54103994511419 31.71930005136303 18.887077999460107 34-35 19.148633994649906 30.34321184868795 30.67950836110147 19.82864579556068 36-37 19.768637689278453 29.06013255908837 31.326705293568597 19.844524458064583 38-39 20.341393153778924 28.839304371415075 31.329549524620344 19.489752950185654 40-41 20.64077587777757 29.047753384312525 31.015733745347152 19.295736992562755 42-43 20.202940715599564 30.172624363485905 30.712387547469717 18.912047373444814 44-45 19.927634061326714 29.79044205619326 30.88603027960321 19.395893602876818 46-47 19.641771375596868 29.342481750628718 31.034311973690926 19.98143490008349 48-49 20.396886411839347 29.306501979805756 30.395703191497635 19.900908416857263 50-51 19.83796255697369 29.254470233499475 31.1335874359148 19.773979773612034 52-53 19.497536536528752 29.724688918186153 31.49866650075338 19.279108044531714 54-55 19.61183106972998 30.051470313229284 30.756064247943854 19.580634369096884 56-57 20.227659789266866 29.122753223788184 30.574722257742387 20.074864729202563 58-59 20.13179681251272 29.004524930394933 30.199324546344876 20.66435371074747 60-61 20.454227891609662 29.321874581316752 30.3570269756345 19.866870551439085 62-63 18.94873828177378 31.28292136105575 30.052462299968184 19.71587805720228 64-65 19.268167559421716 30.396850065990883 30.62133876067979 19.713643613907607 66-67 20.018790652214694 29.05470228285314 30.926504504156433 20.00000256077573 68-69 20.840186664407092 29.618248195682217 29.54129469527651 20.000270444634182 70-71 20.108350614398518 30.49159882904407 29.539435395018394 19.86061516153902 72-73 19.58441799933062 30.505377342948563 30.309395349864342 19.600809307856473 74-75 19.864325575859223 29.570950548938995 30.630606540361395 19.934117334840387 76-77 20.41193951624376 29.64044494740926 29.81538841267506 20.132227123671917 78-79 20.012071360938638 30.51297266393535 29.303467941987243 20.171488033138772 80-81 20.090994426169733 30.274910453875805 30.413568505163376 19.22052661479109 82-83 19.733150005095176 30.423229054596234 30.100219771131798 19.74340116917679 84-85 19.883328737151942 30.58206603359649 29.61979639681889 19.91480883243268 86-87 20.569547928217027 30.44778312931344 28.918548449480475 20.06412049298906 88-89 20.261116918396237 30.370604966126518 29.530934586926 19.837343528551248 90-91 20.412132150956722 30.692919388848495 29.317363733425722 19.57758472676906 92-93 19.93641526663457 30.92652428677447 29.145651196233562 19.991409250357396 94-95 20.004457771703418 30.847343276732353 29.005258022864783 20.142940928699453 96-97 20.29109196335876 30.085825597047805 29.3624992378011 20.26058320179234 98-99 20.977330080741584 30.357092506611487 28.720199817975455 19.94537759467147 100-101 21.027884986118046 31.097195710181364 27.914929881952887 19.959989421747707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13975.0 1 9036.5 2 3498.5 3 2841.5 4 3201.5 5 5093.5 6 6314.0 7 6151.5 8 10688.0 9 11985.5 10 31483.0 11 44318.0 12 26810.0 13 25283.5 14 25727.5 15 21912.5 16 25806.0 17 30520.0 18 38395.0 19 45796.0 20 55996.5 21 70498.0 22 87657.0 23 110747.5 24 141429.0 25 182631.5 26 232692.0 27 295239.0 28 371490.0 29 469935.5 30 598691.5 31 736431.0 32 894065.5 33 1091660.5 34 1312763.0 35 1572884.5 36 1927281.5 37 2080235.0 38 1918253.5 39 1679999.0 40 1480446.0 41 1323692.0 42 1184273.5 43 1096178.0 44 1021799.5 45 966753.0 46 925575.0 47 874534.0 48 820355.5 49 771388.5 50 714094.0 51 653355.0 52 591686.5 53 524555.0 54 457413.5 55 390362.5 56 324196.0 57 256775.5 58 196353.0 59 144833.5 60 101484.5 61 69647.0 62 47203.5 63 30587.0 64 20140.5 65 14134.5 66 9693.0 67 6927.5 68 4855.5 69 3144.5 70 2056.0 71 1303.0 72 857.0 73 594.0 74 444.5 75 324.5 76 213.0 77 141.0 78 105.0 79 69.0 80 49.0 81 40.5 82 34.0 83 38.5 84 28.5 85 18.0 86 17.0 87 12.0 88 8.5 89 6.5 90 2.5 91 1.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055384441475031854 2 0.0042566487813806625 3 0.0072363029283471275 4 0.009671874148370199 5 0.0 6 0.0 7 0.038281034642176026 8 0.001891488293079678 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7605731715205105E-4 18-19 2.0803170736070908E-4 20-21 1.6962585369411664E-4 22-23 1.2801951222197482E-4 24-25 2.480378049300762E-4 26-27 6.40097561109874E-6 28-29 1.9042902443018754E-4 30-31 4.5126878058246125E-4 32-33 4.800731708324056E-6 34-35 6.112931708599298E-4 36-37 3.20048780554937E-6 38-39 0.0 40-41 2.0803170736070912E-5 42-43 1.6962585369411664E-4 44-45 4.000609756936713E-5 46-47 1.632248780830179E-4 48-49 0.0 50-51 3.616551220270789E-4 52-53 0.0 54-55 0.0511213917180401 56-57 0.3606501688561364 58-59 0.1644634668637655 60-61 0.3510183008053355 62-63 0.0025619904883422712 64-65 3.520536586104308E-5 66-67 0.015053494393401467 68-69 0.0012625924392892266 70-71 0.0032676980494659074 72-73 6.240951220821273E-5 74-75 4.608702439991093E-4 76-77 9.1533951238712E-4 78-79 0.011276918782853206 80-81 8.481292684705832E-5 82-83 1.6002439027746852E-5 84-85 3.0884707323551423E-4 86-87 0.0 88-89 0.0 90-91 0.012011430734226789 92-93 3.20048780554937E-6 94-95 0.01014714658749428 96-97 0.010856054636423465 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.1245237E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.492144820480256 #Duplication Level Percentage of deduplicated Percentage of total 1 79.88962334325113 41.93577678187559 2 10.883989961824225 11.426479546014612 3 3.7162377039431282 5.852198632281351 4 1.7076838002896415 3.5855994140956784 5 0.9651728895965427 2.5331997548751564 6 0.5899434444327292 1.8580438032633455 7 0.4093512611173228 1.5041407976711714 8 0.29220509901432956 1.227077789979437 9 0.21532571549929555 1.0172617777406296 >10 1.1727746804689156 11.36616318715672 >50 0.09293862117918852 3.3699273454325356 >100 0.05647431854734564 5.526120088212565 >500 0.004467551913051454 1.59686836412584 >1k 0.003194255937510238 3.2834149538459423 >5k 3.606319424494774E-4 1.310448260335537 >10k+ 2.567210432644322E-4 2.607279503093679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66424 0.2125892019958114 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 53505 0.17124210003591908 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 39439 0.1262240385630616 No Hit GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT 35391 0.11326846392619777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002157128780940276 3.2004878055493706E-6 0.0 0.0 0.0 2 0.0021827326833846706 6.400975611098741E-6 0.0 9.601463416648112E-6 3.2004878055493706E-6 3 0.00223714097607901 6.400975611098741E-6 0.0 1.2801951222197482E-5 3.2004878055493706E-6 4 0.0024931800005229595 6.400975611098741E-6 0.0 1.9202926833296224E-5 3.2004878055493706E-6 5 0.0025283853663840027 6.400975611098741E-6 0.0 1.9202926833296224E-5 3.2004878055493706E-6 6 0.002624400000550484 6.400975611098741E-6 0.0 2.2403414638845593E-5 3.2004878055493706E-6 7 0.002710813171300317 6.400975611098741E-6 0.0 2.2403414638845593E-5 6.400975611098741E-6 8 0.0028004268298556993 6.400975611098741E-6 0.0 2.2403414638845593E-5 6.400975611098741E-6 9 0.002912443903049927 6.400975611098741E-6 0.0 2.5603902444394964E-5 6.400975611098741E-6 10-11 0.003406919269007305 6.400975611098741E-6 0.0 3.040463415271902E-5 6.400975611098741E-6 12-13 0.0040870229276865466 6.400975611098741E-6 0.0 3.840585366659245E-5 6.400975611098741E-6 14-15 0.005074373415698527 6.400975611098741E-6 0.0 4.000609756936713E-5 6.400975611098741E-6 16-17 0.006192943903738032 6.400975611098741E-6 0.0 5.120780488878993E-5 6.400975611098741E-6 18-19 0.007423531464971765 6.400975611098741E-6 0.0 6.881048781931146E-5 8.001219513873426E-6 20-21 0.008943763172607717 6.400975611098741E-6 0.0 7.521146343041021E-5 1.2801951222197482E-5 22-23 0.010249562197271859 6.400975611098741E-6 0.0 9.441439026370644E-5 1.2801951222197482E-5 24-25 0.011539358782908255 6.400975611098741E-6 0.0 1.0881658538867861E-4 1.2801951222197482E-5 26-27 0.01279074951487806 6.400975611098741E-6 0.0 1.2161853661087608E-4 1.2801951222197482E-5 28-29 0.014221367563958628 9.601463416648112E-6 0.0 1.3282024393029888E-4 1.2801951222197482E-5 30-31 0.01564238414962255 1.2801951222197482E-5 0.0 1.536234146663698E-4 1.2801951222197482E-5 32-33 0.01707780293041144 1.2801951222197482E-5 0.0 1.72826341499666E-4 1.2801951222197482E-5 34-35 0.01879486463808868 1.2801951222197482E-5 0.0 2.1283243906903312E-4 1.2801951222197482E-5 36-37 0.020772766101918187 1.6002439027746852E-5 0.0 2.6884097566614717E-4 1.2801951222197482E-5 38-39 0.023016308053608298 1.760268293052154E-5 0.0 2.880439024994433E-4 1.9202926833296224E-5 40-41 0.025463080980950792 1.9202926833296224E-5 0.0 3.0884707323551423E-4 1.9202926833296224E-5 42-43 0.028551551713305937 1.9202926833296224E-5 0.0 3.2965024397158514E-4 1.9202926833296224E-5 44-45 0.03206568732379914 2.080317073607091E-5 0.0 3.4085195129100797E-4 2.080317073607091E-5 46-47 0.036303133178346506 2.5603902444394964E-5 0.0 3.6005487812430423E-4 2.2403414638845593E-5 48-49 0.041259088545239714 2.7204146347169652E-5 0.0 3.7125658544372696E-4 2.2403414638845593E-5 50-51 0.04736561927822791 2.8804390249944336E-5 0.0 4.000609756936713E-4 2.5603902444394964E-5 52-53 0.05522761757255994 3.6805609763817764E-5 0.0 4.1286292691586877E-4 2.5603902444394964E-5 54-55 0.06456504074525023 4.000609756936713E-5 0.0 4.384668293602638E-4 2.5603902444394964E-5 56-57 0.0765780717233798 4.4806829277691185E-5 0.0 4.8327365863795493E-4 2.5603902444394964E-5 58-59 0.09236927855596039 4.4806829277691185E-5 0.0 5.280804879156461E-4 2.5603902444394964E-5 60-61 0.11215309392596381 4.4806829277691185E-5 0.0 5.760878049988866E-4 2.5603902444394964E-5 62-63 0.13972529637077163 4.800731708324056E-5 0.0 6.016917074432817E-4 2.8804390249944336E-5 64-65 0.1748266463781344 5.600853659711399E-5 0.0 6.112931708599298E-4 2.8804390249944336E-5 66-67 0.2204079937047685 6.080926830543804E-5 0.0 6.272956098876766E-4 2.8804390249944336E-5 68-69 0.2810252327418736 6.56100000137621E-5 0.0 6.336965854987753E-4 2.8804390249944336E-5 70-71 0.36027891227069264 6.721024391653678E-5 0.0 6.432980489154235E-4 2.8804390249944336E-5 72-73 0.4629473605849109 6.721024391653678E-5 0.0 6.60900731845945E-4 2.8804390249944336E-5 74-75 0.5937977042708942 6.721024391653678E-5 0.0 6.769031708736919E-4 2.8804390249944336E-5 76-77 0.7570097804026898 6.881048781931146E-5 0.0 6.849043903875653E-4 2.8804390249944336E-5 78-79 0.9580132165424124 7.68117073331849E-5 0.0 6.993065855125375E-4 2.8804390249944336E-5 80-81 1.2051644863503514 7.841195123595958E-5 0.0 7.089080489291856E-4 2.8804390249944336E-5 82-83 1.5089179832433341 8.001219513873427E-5 0.0 7.201097562486084E-4 3.040463415271902E-5 84-85 1.8855065813711063 8.001219513873427E-5 0.0 7.313114635680312E-4 3.2004878055493705E-5 86-87 2.3295310578057062 8.161243904150895E-5 0.0 7.409129269846793E-4 3.6805609763817764E-5 88-89 2.8296936905935457 8.6413170749833E-5 0.0 7.505143904013275E-4 3.840585366659245E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGC 5135 0.0 35.81521 1 CGGCGCT 1745 0.0 34.041725 1 GACGGCC 6630 0.0 29.443312 2 CGGCGAG 1760 0.0 26.191238 1 CCGACGG 760 0.0 25.637001 1 ACGGCCA 8010 0.0 24.547667 3 TATGCCG 6545 0.0 23.462418 46-47 CGGCCAG 10600 0.0 23.267967 1 ATGCCGT 6605 0.0 21.991594 46-47 TGCCGTC 6885 0.0 21.866833 48-49 CGTATGC 7490 0.0 20.343748 44-45 GCCGTCT 7140 0.0 20.187897 48-49 CTCGTAT 7045 0.0 19.87689 42-43 CTGGAAT 90590 0.0 19.871319 1 CCGTCTT 7600 0.0 19.587719 50-51 TCTCGTA 6830 0.0 19.564337 40-41 TCGTATG 7910 0.0 18.75344 42-43 CGGCGAT 460 0.0 18.595661 1 CGTCTTC 8160 0.0 17.688864 50-51 GCGACGG 850 0.0 17.331644 1 >>END_MODULE