FastQCFastQC Report
Fri 27 May 2016
SRR1233114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073421
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC639900.4245220776358598No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG337300.22377136550488438Illumina Single End PCR Primer 1 (100% over 50bp)
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA228200.15139230835521678No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA187540.12441767532400243No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG152680.10129087484519939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGAAT576600.040.5577741
GACGGCC37350.039.8285142
CGACGGC44900.032.5983621
CGCCGTA91500.029.6454546-47
GTCGCCG89700.029.62427344-45
CCGTATC93450.029.16771948-49
GAATATG876600.027.5576254
GGAATAT961150.026.4264243
TGGAATA960650.026.3987452
TCGGTGG105550.024.93832638-39
CGGCCAG59500.024.61574
TCTCGGT107400.024.37314636-37
TGGTCGC104200.024.36024742-43
CCCGTAT12750.024.0704151
GATCTCG117150.023.91739834-35
CCGTTCG8800.023.6188933
ACGGCCA63650.023.2292653
CGTATCA93300.023.17811848-49
TATGGCG838550.022.9194187
TCGCCGT90300.022.8792444-45
Produced by FastQC (version 0.11.5)