FastQCFastQC Report
Fri 27 May 2016
SRR1233114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073421
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC570280.3783348186188125No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC466890.3097438862750533TruSeq Adapter, Index 11 (100% over 50bp)
AGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAA201180.13346671601622487No Hit
CGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTT171280.11363047578913904No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA167680.11124216592902167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCC43100.044.74352
CTGGAAT515050.040.3321231
CGACGGC51900.037.053541
ACGGCCA67200.028.4850463
TATGCCG106350.027.51214246-47
GAATATG767400.027.4135344
CGGCCAG70450.027.1035544
TGCCGTC110150.026.56301548-49
TGGAATA842450.026.561352
GGAATAT849350.026.2672773
CCGTCTT111950.026.26320550-51
CTCGTAT111750.026.18269542-43
CGTATGC111950.026.15713544-45
CCCGTAT12600.026.0671921
ATCTCGT121300.024.04300340-41
TATGGCG725900.022.6663847
TGGCGAG713750.022.4200219
ATGCCGT108300.022.36778346-47
ATGGCGA737300.022.2772678
TCTCGTA108300.022.1704240-41
Produced by FastQC (version 0.11.5)