FastQCFastQC Report
Fri 27 May 2016
SRR1233113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72709612
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC20498832.8192737433394637No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA9534871.3113630698510672No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC7003580.9632261550233551No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT6959910.9572200715360715No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA5567010.7656498015695641No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG5491560.7552729066962977No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG5271760.7250430658328916No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT4283950.5891862000308845No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG3869150.532137346572555No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT3831050.5268973241117006No Hit
CCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAA3642930.5010245412944853No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA2892190.397772718138009No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT2789530.3836535395072662No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC2619300.3602412291788877No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG2554180.3512850543061624No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA2280520.3136476646306406No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA2232670.3070666915400401No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT2207120.30355271322311556No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT2152280.2960103816810355No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA2064680.28396245602300835No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT2058290.2830836176102824No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1993690.274198960104477No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT1961220.2697332506739274No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG1920230.26409575669307656No Hit
CCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGA1896050.26077019913130606No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG1871300.2573662475327196No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA1822170.2506092316927781No Hit
CTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTT1791980.2464570984094923No Hit
CAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGT1733320.2383893892873476No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT1534870.21109588646959088No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG1523730.20956376441673216No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG1443240.19849370121793525No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC1436350.19754609610624796No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1381330.18997900855254185No Hit
TAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTT1319670.18149869923662915No Hit
CGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAAGAC1280680.17613627205162366No Hit
ATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAA1133590.15590648455117598No Hit
ATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATG1129190.15530133760031617No Hit
CGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTAC1114900.153335985344001No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA1088430.14969547630098756No Hit
CTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAACCTTGGA1087840.14961433159621315No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1076350.14803407285408152No Hit
CACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTT1069730.14712360175983336No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC1065430.1465322081487658No Hit
CGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAA1019220.14017678983075857No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC1009350.13881933519326164No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA998550.13733397449569668No Hit
CTTCTTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGA991610.13637949271411323No Hit
CTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATGAAATA936930.12885916651570084No Hit
TTTTTTTCTATTGTATCACGGTCTTTTATATATTATATTATTTGATACTT930900.12802984012622706No Hit
GCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATA911870.12541257956375837No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA902530.1241280176271605No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA884090.12159190176946619No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA852510.11724859706306781No Hit
GTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATA833250.1145997038190769No Hit
CAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCA804490.1106442432948205No Hit
CTTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGAAAA779230.10717014966329348No Hit
TTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATT760990.10466154048518372No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA744530.10239774075537632No Hit
CACCGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAA742990.1021859393225754No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT729500.10033061378459839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCC79850.047.7853131
CTCCGCT65100.043.438521
CGTCCGT23000.041.053471
CACGGTC2621700.039.2869261
CGGGTGC103650.037.7939571
CGGCCAG421450.034.7669031
CCCGCTG93300.033.838341
CTTTTAT6797700.033.6191181
TTATATA6874950.032.465084
TATTATA7021700.031.8395069
TATATAT7238350.030.8596845
TTTTATA7332600.030.7384762
TTTATAT7343750.030.6009063
ACGGTCT3410000.030.0960162
CGGTGCT82500.029.5227761
CGTCCGC27300.028.9113311
CCGCTAC62000.028.8189281
CTGGAAT963950.026.7189031
TCACGGT1637100.026.0011792
CGGTCTT4100950.025.54643
Produced by FastQC (version 0.11.5)