FastQCFastQC Report
Fri 27 May 2016
SRR1233113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72709612
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC20651522.8402737178682784No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA10550811.4510887501366394No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT6687410.91974222060214No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC6665370.916710984511924No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG5779200.7948330132747785No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG5224780.7185817467984839No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA5154130.7088650122352461No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG4248170.5842652550532109No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT3963810.5451562580199163No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT3929400.5404237337973966No Hit
CCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAA3726860.5125677193821361No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA3180610.43744010076686973No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT2792490.3840606383651174No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT2611060.3591079539800047No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC2602110.3578770300685967No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG2500260.3438692534901713No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT2432500.3345499904469302No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA2383580.32782185662055247No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT2300910.3164519706142841No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT2238110.30781487322473955No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA2188930.3010509807149019No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT1994990.27437775352177646No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA1988650.2735057917789466No Hit
CAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGT1988240.27344940308579835No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA1926840.26500485245334554No Hit
CCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGA1907330.2623215758598739No Hit
CTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTT1842160.2533585243172526No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG1816530.24983354332849417No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG1808570.2487387774810296No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC1606910.22100379245594107No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT1593980.21922548562080071No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG1592790.21906182087727274No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1581940.21756958350981162No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG1456530.20032152007632775No Hit
CGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAAGAC1452120.19971499779148869No Hit
TAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTT1403690.19305425533009307No Hit
ATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATG1298770.17862425122004502No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC1266260.17415304045357854No Hit
ATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAA1229110.16904367472075083No Hit
CACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTT1216520.16731212924090422No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1167870.1606211294319656No Hit
CGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTAC1136860.15635621876238318No Hit
CGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAA1076960.14811796822681436No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA1074190.14773700071456852No Hit
CTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAACCTTGGA1061780.14603021124634802No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC1046330.14390532024844252No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA1012540.13925806673263502No Hit
TTTTTTTCTATTGTATCACGGTCTTTTATATATTATATTATTTGATACTT982110.13507292543384772No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA976990.13436875443648358No Hit
CTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATGAAATA928500.1276997599712126No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA912040.12543596024140521No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA908280.12491883466521593No Hit
GCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATA892120.12269629495478535No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA865950.11909704593114868No Hit
TTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATT850260.11693914691774177No Hit
GTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATA846140.11637250931830032No Hit
CAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCA842000.1158031210509004No Hit
CTTCTTTGGACTTGATCAAGGAAAGAAGATCTTTGAAGCCAGAAGTTAGA841970.11579899504896272No Hit
CACCGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAA838830.11536714017948548No Hit
GTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATA809560.1113415376222885No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT781900.10753736383574705No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT762720.10489947326358996No Hit
TTTTTTTTCTATTGTATCACGGTCTTTTATATATTATATTATTTGATACT732440.10073496197449108No Hit
TAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTT730520.10047089785047952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT62650.053.992221
CGCCGCC75300.044.4801671
CTCCGCT59450.039.2375531
CACGGTC2804950.039.2253651
CGTCCGT21250.036.6654661
CGGGTGC85550.036.4297451
GCGGGTG95000.035.2804952
CGTCCGC22450.034.705621
CCGCTAC63350.034.047211
CGGCCAG417450.032.2186741
CCCGCTG85400.032.0432781
CGGGTGT111950.031.2091713
TATTATA7572450.031.184589
CTTTTAT6996600.030.8100451
CCGTCAC64250.030.5386051
ACGGTCT3629500.030.1693842
CGCCGGT25000.030.025441
TTATATA7231750.029.7029174
TATATAT7764250.029.49455
CGGTGCT78850.029.4029181
Produced by FastQC (version 0.11.5)