FastQCFastQC Report
Fri 27 May 2016
SRR1233112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68567500
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC5393000.7865242279505598No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC2326440.339291938600649No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1658020.2418084369416998No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT1410500.20570970211835055No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1131920.165081124439421No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1013920.14787180515550372No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT982910.14334925438436577No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA951910.13882816203011633No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT864310.1260524300871404No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT778210.11349546067743463No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA738370.10768512779377987No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA738060.10763991687023734No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT730930.10660006562875998No Hit
TTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACAA705140.10283880847340213No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA700060.10209793269406059No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA694850.10133809749516898No Hit
GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA693700.10117037955299524No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA690640.10072410398512416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT1828800.034.0906261
TATTATA1781300.030.8219689
TTATATA1823650.030.5837274
TTTTATA2082650.028.2397672
TTTATAT2238900.025.5686443
CGGCGAG55000.024.0018181
TATATAT2351850.023.6578165
CACGGTC328550.022.5121671
CTGGAAT2174700.019.7820421
GGTGTCC1313150.018.821113
GGAAAAC3667200.018.1209031
TGTCCAA1414900.017.7285085
GTGTCCA1410000.017.6570744
TGGTGTC1399850.017.6349932
CTGGTGT1505500.016.8722691
CGGCGCT89750.016.6733261
GTCCAAG1530400.016.6544156
CGCCAGT92000.016.1619511
TGATACT1781600.015.93347320-21
TTGATAC1717550.015.84930520-21
Produced by FastQC (version 0.11.5)