##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68567500 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.636368250264336 33.0 31.0 34.0 30.0 34.0 2 31.892072497903527 34.0 31.0 34.0 30.0 34.0 3 31.919990082765157 34.0 31.0 34.0 30.0 34.0 4 35.40145143107157 37.0 35.0 37.0 33.0 37.0 5 35.40514029241259 37.0 35.0 37.0 33.0 37.0 6 35.419870507164475 37.0 35.0 37.0 33.0 37.0 7 35.356167309585445 37.0 35.0 37.0 33.0 37.0 8 35.332378357093376 37.0 35.0 37.0 33.0 37.0 9 37.0144078754512 39.0 37.0 39.0 33.0 39.0 10-11 37.032307674918876 39.0 37.0 39.0 33.5 39.0 12-13 36.946339373609945 39.0 37.0 39.0 33.0 39.0 14-15 38.237387078426366 40.0 38.0 41.0 33.0 41.0 16-17 38.104897407663984 40.0 38.0 41.0 33.0 41.0 18-19 38.10712925948883 40.0 38.0 41.0 33.0 41.0 20-21 38.20732464359938 40.0 38.0 41.0 33.5 41.0 22-23 38.16071473365662 40.0 38.0 41.0 33.0 41.0 24-25 37.508620512633534 40.0 37.0 41.0 31.5 41.0 26-27 36.50281318408867 39.0 36.0 41.0 29.0 41.0 28-29 36.1881745506253 39.0 35.5 41.0 27.0 41.0 30-31 35.95282756407919 39.0 35.0 41.0 27.0 41.0 32-33 35.879297903525725 39.0 35.0 41.0 27.0 41.0 34-35 35.738429773580776 39.0 35.0 41.0 27.0 41.0 36-37 35.6488068910198 39.0 35.0 41.0 26.5 41.0 38-39 35.5122503883035 39.0 34.0 41.0 25.5 41.0 40-41 35.59816413752871 39.0 34.5 41.0 26.5 41.0 42-43 35.7018494986692 39.0 35.0 41.0 26.5 41.0 44-45 35.594943150909685 39.0 35.0 41.0 26.0 41.0 46-47 35.49459007547307 39.0 35.0 41.0 26.0 41.0 48-49 35.39853212527801 39.0 34.0 41.0 26.0 41.0 50-51 35.3244702592336 39.0 34.0 41.0 25.5 41.0 52-53 35.23244225033726 39.0 34.0 41.0 25.0 41.0 54-55 35.104041200277095 39.0 34.0 41.0 24.5 41.0 56-57 34.932822816932216 39.0 34.0 41.0 24.0 41.0 58-59 34.72089746600066 38.0 34.0 40.5 24.0 41.0 60-61 34.50052724687352 38.0 33.0 40.0 23.0 41.0 62-63 34.320466226710906 38.0 33.0 40.0 23.0 41.0 64-65 34.05310165894921 38.0 33.0 40.0 22.5 41.0 66-67 33.646849155941226 37.0 32.0 40.0 22.0 41.0 68-69 33.31002068035148 37.0 32.0 39.5 21.5 41.0 70-71 33.01218974732927 36.5 32.0 39.0 21.0 41.0 72-73 32.71664443796259 36.0 31.5 39.0 20.5 41.0 74-75 32.293918386990924 35.5 31.0 39.0 19.5 40.0 76-77 31.940299106719657 35.0 31.0 38.0 18.0 40.0 78-79 31.588153002515767 35.0 31.0 37.0 18.0 39.0 80-81 31.227157939256934 35.0 31.0 37.0 17.5 39.0 82-83 30.87514085390309 35.0 31.0 36.0 13.0 39.0 84-85 30.537907441572173 35.0 30.0 36.0 11.5 37.0 86-87 30.155135136908886 34.0 30.0 35.5 7.5 37.0 88-89 29.816208874466767 34.0 30.0 35.0 6.0 37.0 90-91 29.538214175812158 34.0 29.0 35.0 2.0 36.0 92-93 29.271052699894263 34.0 29.0 35.0 2.0 36.0 94-95 28.990973836001018 34.0 29.0 35.0 2.0 36.0 96-97 28.64066030553834 34.0 29.0 35.0 2.0 36.0 98-99 28.31972825318117 34.0 29.0 35.0 2.0 35.0 100-101 27.27272380500966 33.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2351731120312266 1101 2 0.3416568714415895 1101 3 0.3427138980001452 1101 4 0.3112283010805186 1101 5 0.2990452852594032 1101 6 0.30412225249745006 1101 7 0.32729752012615165 1101 8 0.33893288746313033 1101 9 0.40332300631504836 1101 10-11 0.37483908481277695 1101 12-13 0.3865813800637099 1101 14-15 0.5210160968467861 1101 16-17 0.4614084878382698 1101 18-19 0.48941082741327335 1101 20-21 0.4395451074172314 1101 22-23 0.4771902480630885 1101 24-25 0.5173466608030992 1101 26-27 0.46377095836256643 1101 28-29 0.3526329014326208 1101 30-31 0.29067045955668647 1101 32-33 0.3152443072105058 1101 34-35 0.3976336178984923 1101 36-37 0.3261980521017662 1101 38-39 0.33439213603869433 1101 40-41 0.41325201849577553 1101 42-43 0.39003581526020525 1101 44-45 0.3249226586150442 1101 46-47 0.35105607186974197 1101 48-49 0.402016322750292 1101 50-51 0.4928494326043591 1101 52-53 0.4498510744456752 1101 54-55 0.48821179856011554 1101 56-57 0.559987462832872 1101 58-59 0.5417526846188991 1101 60-61 0.4928394179253104 1101 62-63 0.5588800851599132 1101 64-65 0.5586803451265396 1101 66-67 0.4871646511793628 1101 68-69 0.5196264163952549 1101 70-71 0.5291329166505605 1101 72-73 0.5507610120672268 1101 74-75 0.5316296822626967 1101 76-77 0.553663887444916 1101 78-79 0.5132677649899406 1101 80-81 0.4483555153583971 1101 82-83 0.4181546344500511 1101 84-85 0.35304381140926466 1101 86-87 0.3387072920400449 1101 88-89 0.42803739117673345 1101 90-91 0.4076805528519216 1101 92-93 0.3872613056888774 1101 94-95 0.47965972082553066 1101 96-97 0.4751465570482125 1101 98-99 0.4162426757218931 1101 100-101 0.4519331963338864 1102 1 0.11088625848622868 1102 2 0.14523922018153357 1102 3 0.17800907862809723 1102 4 0.1601903338295827 1102 5 0.1756301422569564 1102 6 0.22541485883227352 1102 7 0.23885936995470303 1102 8 0.22146753067484326 1102 9 0.25949106711932046 1102 10-11 0.300902681775149 1102 12-13 0.3049572000972276 1102 14-15 0.3509422473287245 1102 16-17 0.29225964475428867 1102 18-19 0.26300952904719566 1102 20-21 0.31923259503650314 1102 22-23 0.31046343454170966 1102 24-25 0.3748934622236675 1102 26-27 0.32816560654106297 1102 28-29 0.2599992169261114 1102 30-31 0.3440288538777736 1102 32-33 0.365249803816198 1102 34-35 0.28241586879820346 1102 36-37 0.19654869000664377 1102 38-39 0.22581119863516363 1102 40-41 0.27516134536600134 1102 42-43 0.2880017165824924 1102 44-45 0.3915479150164529 1102 46-47 0.24975241840405005 1102 48-49 0.24240043160170188 1102 50-51 0.26855791148034314 1102 52-53 0.38056855163856085 1102 54-55 0.28895786439856863 1102 56-57 0.2763257426672183 1102 58-59 0.3042820917440494 1102 60-61 0.29058785069796755 1102 62-63 0.22795281040890814 1102 64-65 0.2284426860903963 1102 66-67 0.2841247213751714 1102 68-69 0.34063303497900677 1102 70-71 0.3168956297923984 1102 72-73 0.17913930141455126 1102 74-75 0.15919966284026543 1102 76-77 0.28679916328301047 1102 78-79 0.25996800205319204 1102 80-81 0.1953992508798983 1102 82-83 0.15801603431687283 1102 84-85 0.2047846083826954 1102 86-87 0.24378096394068294 1102 88-89 0.17022581123914549 1102 90-91 0.2007847272225476 1102 92-93 0.17376439652350584 1102 94-95 0.19578381999362193 1102 96-97 0.18014200461062657 1102 98-99 0.15417980306358814 1102 100-101 0.09743639904273849 1103 1 0.20015387291386944 1103 2 0.1927268747731823 1103 3 0.23110680226648483 1103 4 0.14508101552315367 1103 5 0.14209935316435462 1103 6 0.1989483977409492 1103 7 0.2122264588784475 1103 8 0.17221296772054728 1103 9 0.19163035279787266 1103 10-11 0.15203126044507087 1103 12-13 0.20605657591428894 1103 14-15 0.3033890199289928 1103 16-17 0.2789695005141226 1103 18-19 0.2330214085041078 1103 20-21 0.25769017010009776 1103 22-23 0.2942473920727551 1103 24-25 0.31531143218496993 1103 26-27 0.32254893498664927 1103 28-29 0.4069905969901271 1103 30-31 0.4629837991171968 1103 32-33 0.41727155728391097 1103 34-35 0.4827302417500121 1103 36-37 0.4647872050980979 1103 38-39 0.46285169190257136 1103 40-41 0.3806560690007714 1103 42-43 0.4147213711170963 1103 44-45 0.38180575103567804 1103 46-47 0.3435128899487765 1103 48-49 0.2865866583833565 1103 50-51 0.31847037267799294 1103 52-53 0.3469597418600401 1103 54-55 0.26935698928252094 1103 56-57 0.281538391432683 1103 58-59 0.25614087140265696 1103 60-61 0.20099483551404518 1103 62-63 0.32415997562365106 1103 64-65 0.36388803783638224 1103 66-67 0.3037550468192407 1103 68-69 0.30815446593690154 1103 70-71 0.3314362290199071 1103 72-73 0.24482441142089328 1103 74-75 0.24397536524415386 1103 76-77 0.25393308275568316 1103 78-79 0.24219611330428137 1103 80-81 0.2779439067556986 1103 82-83 0.2882834738829416 1103 84-85 0.2839352950564731 1103 86-87 0.2654530244024542 1103 88-89 0.2827958952107359 1103 90-91 0.2980654530255009 1103 92-93 0.3285669028598086 1103 94-95 0.36124477047306414 1103 96-97 0.3694283616461185 1103 98-99 0.36728512776971556 1103 100-101 0.30009918994174356 1104 1 0.12104163479665075 1104 2 0.16289979020339374 1104 3 0.16203672198846775 1104 4 0.0793402816397446 1104 5 0.0727930932400227 1104 6 0.07216743444992346 1104 7 0.01922925637590822 1104 8 0.04016140984933969 1104 9 0.0855407271175892 1104 10-11 0.10049372607108609 1104 12-13 0.07601130031286374 1104 14-15 0.08938273584551126 1104 16-17 0.0976309994968858 1104 18-19 0.13388996504779271 1104 20-21 0.08884454255954921 1104 22-23 0.1279281416383995 1104 24-25 0.1009751096765541 1104 26-27 0.083445337016407 1104 28-29 -0.0070476961287582185 1104 30-31 0.006994592151286838 1104 32-33 0.045011800868778096 1104 34-35 0.1944387595434236 1104 36-37 0.03558685178423815 1104 38-39 0.027924496031801027 1104 40-41 -0.051872155712651136 1104 42-43 0.06921550204526028 1104 44-45 0.03378124837630736 1104 46-47 -0.0762252707317046 1104 48-49 0.09511030610855187 1104 50-51 -0.017098803028034126 1104 52-53 -0.03133028100774027 1104 54-55 0.05116193844088457 1104 56-57 0.035924546179259664 1104 58-59 0.019149401314088266 1104 60-61 -0.06587217890601238 1104 62-63 0.09033383094688219 1104 64-65 0.14032108596509119 1104 66-67 0.005572943416787268 1104 68-69 0.054525196535117004 1104 70-71 -0.011001057969117767 1104 72-73 0.03758691014834881 1104 74-75 0.09318989306056125 1104 76-77 0.1374105109757231 1104 78-79 0.048298440698939515 1104 80-81 0.0899368388061319 1104 82-83 -0.007104160357869205 1104 84-85 -0.0017585502868016079 1104 86-87 0.018986753306734272 1104 88-89 0.02764941830149681 1104 90-91 0.02282263705145482 1104 92-93 0.015607742952060022 1104 94-95 0.04651385497264471 1104 96-97 0.08321140480447653 1104 98-99 0.11552672941130382 1104 100-101 0.1047496382550257 1105 1 0.0698364964070528 1105 2 0.10511507656905295 1105 3 0.10800653923475423 1105 4 0.03138632561122279 1105 5 0.060499495046002494 1105 6 0.12336641303911478 1105 7 0.11470279358093194 1105 8 0.0996367705806449 1105 9 0.06970978600551803 1105 10-11 0.04009324836462014 1105 12-13 0.07958501864981571 1105 14-15 0.15045989002845772 1105 16-17 0.11280954893096862 1105 18-19 0.04204355132731763 1105 20-21 0.03722234181793027 1105 22-23 0.1306623721402218 1105 24-25 0.13094936943743107 1105 26-27 0.20352996344504248 1105 28-29 0.20701679974484222 1105 30-31 0.2681312556133051 1105 32-33 0.18781238604981354 1105 34-35 0.20297112472032808 1105 36-37 0.19100875156487263 1105 38-39 0.10977481180876225 1105 40-41 0.15158036801472718 1105 42-43 0.20658836139052994 1105 44-45 0.15346983867404873 1105 46-47 0.13351957965771533 1105 48-49 0.1368817398195361 1105 50-51 0.15881432607947232 1105 52-53 0.20016915982134975 1105 54-55 0.22630559767621605 1105 56-57 0.13545616378282688 1105 58-59 0.1415775931591341 1105 60-61 0.1318554135791885 1105 62-63 0.1497704440922405 1105 64-65 0.16969093248116707 1105 66-67 0.14509322615592168 1105 68-69 0.10156356396264954 1105 70-71 0.044637443976007773 1105 72-73 0.05544324755945951 1105 74-75 0.09107116559054163 1105 76-77 0.03467195573597692 1105 78-79 0.03961506584195007 1105 80-81 0.08601234089626786 1105 82-83 0.07140847226328972 1105 84-85 0.05524538906116305 1105 86-87 0.08912419586545539 1105 88-89 0.05324989327147378 1105 90-91 0.10682893448154829 1105 92-93 0.10480782848099679 1105 94-95 0.0618667298435156 1105 96-97 0.025717715447555634 1105 98-99 0.07831940611688992 1105 100-101 -0.030333136075075373 1106 1 -0.0024883918409983607 1106 2 0.041327086583898165 1106 3 0.03142160794928728 1106 4 -0.0016803322331782056 1106 5 0.041811956899884706 1106 6 0.03553320422835071 1106 7 0.06314398026461276 1106 8 0.0708250055148909 1106 9 0.08608142095864224 1106 10-11 0.037496830544846205 1106 12-13 0.015150089077607731 1106 14-15 0.0014692810126533118 1106 16-17 0.07206528245030341 1106 18-19 0.04607360439349151 1106 20-21 0.006814695357526546 1106 22-23 0.016165629879090204 1106 24-25 -0.011724621545745606 1106 26-27 0.025612998534676024 1106 28-29 -0.04367848768306004 1106 30-31 0.049664872703189644 1106 32-33 0.006045195600648867 1106 34-35 0.04887222701749039 1106 36-37 0.07064206285567565 1106 38-39 -0.017787977565077995 1106 40-41 0.01807185879736295 1106 42-43 0.028339909651791118 1106 44-45 -0.03900140107581507 1106 46-47 -0.00785364185868076 1106 48-49 0.029204677323384942 1106 50-51 0.013047166350688144 1106 52-53 -0.010137417504147095 1106 54-55 0.052863500277645414 1106 56-57 0.030010995790256345 1106 58-59 -0.008109856764342283 1106 60-61 -0.01545420361991745 1106 62-63 -0.01827647997708226 1106 64-65 -0.030895373869569198 1106 66-67 -0.06066310503236849 1106 68-69 -0.0031609536453132137 1106 70-71 -0.049805771959526624 1106 72-73 -0.040990574109777356 1106 74-75 -0.05230102141045734 1106 76-77 -0.06518221513997347 1106 78-79 -0.035781126642504546 1106 80-81 -0.01832618510046302 1106 82-83 -0.13418889500005093 1106 84-85 -0.012411132133998137 1106 86-87 -0.03614860899592642 1106 88-89 -0.03643767283806554 1106 90-91 -0.027064258892878 1106 92-93 0.007152268544945883 1106 94-95 -0.06703349075245058 1106 96-97 -0.011097351402749922 1106 98-99 -0.07875690501650467 1106 100-101 -0.11823916663029976 1107 1 -0.016581749859369666 1107 2 0.044939593118677124 1107 3 0.05310806975516158 1107 4 0.004699784206422919 1107 5 0.04235211977361075 1107 6 0.03649155211311239 1107 7 0.049345583807784976 1107 8 0.039660720818787354 1107 9 0.06994347560160463 1107 10-11 0.028177087246781696 1107 12-13 -0.11366739740306286 1107 14-15 -0.21497078633154132 1107 16-17 -0.2127894004192683 1107 18-19 0.05177556581356413 1107 20-21 0.05717631213803287 1107 22-23 -0.008825476583915304 1107 24-25 -0.10269278157832673 1107 26-27 -0.19937716826519392 1107 28-29 -0.12225728311859285 1107 30-31 -0.11973836658157211 1107 32-33 -0.07554003685577015 1107 34-35 0.07421476447488118 1107 36-37 -0.022840112212996644 1107 38-39 0.031785564934587285 1107 40-41 0.0455718564052674 1107 42-43 0.02235113016575241 1107 44-45 0.04506581690682765 1107 46-47 0.009114149759518853 1107 48-49 -0.007254149615008032 1107 50-51 -0.0019417350839603387 1107 52-53 -0.020066780710322973 1107 54-55 -0.034001649155960934 1107 56-57 -0.040728201305654466 1107 58-59 -0.06638873846828375 1107 60-61 -0.053780575189293245 1107 62-63 -0.10867153749844505 1107 64-65 -0.06782288634323663 1107 66-67 -0.04487693131883219 1107 68-69 0.020276474358944085 1107 70-71 -0.03208281125561996 1107 72-73 -0.13481154265667783 1107 74-75 -0.06145217123135893 1107 76-77 0.011233563129362523 1107 78-79 -0.061023660464485374 1107 80-81 -0.0858734522936686 1107 82-83 -0.04559616856307258 1107 84-85 -0.05209015200031075 1107 86-87 -0.028519105764839736 1107 88-89 -0.0655858436493908 1107 90-91 -0.03860033754973813 1107 92-93 -0.06230038364029866 1107 94-95 -0.048768742250217656 1107 96-97 -0.03302838795289631 1107 98-99 -0.07135011129126312 1107 100-101 -0.19604915144552493 1108 1 -0.08767493881715183 1108 2 0.0016048146362983573 1108 3 0.0028450336246237384 1108 4 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-1.659688910193438 2216 84-85 -1.650486002617086 2216 86-87 -1.6703548441610145 2216 88-89 -1.6917274469789092 2216 90-91 -1.7319501402934776 2216 92-93 -1.8744957042588588 2216 94-95 -1.940772372355351 2216 96-97 -1.9457028025932637 2216 98-99 -1.9251831646413358 2216 100-101 -1.8153120858278733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 321356.0 3 90567.0 4 90689.0 5 104256.0 6 136050.0 7 167755.0 8 199790.0 9 239870.0 10 239802.0 11 233428.0 12 246687.0 13 267928.0 14 291713.0 15 314467.0 16 339995.0 17 340802.0 18 323334.0 19 331838.0 20 349698.0 21 379178.0 22 423824.0 23 488649.0 24 581625.0 25 705828.0 26 836007.0 27 976270.0 28 1154067.0 29 1365598.0 30 1635495.0 31 1977984.0 32 2436670.0 33 3043410.0 34 3883674.0 35 5104096.0 36 7039895.0 37 1.0804709E7 38 1.5533274E7 39 5549832.0 40 17390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.82980863042727 17.38846886840422 21.21487878651403 34.566843714654475 2 18.954336110919165 23.26880081453475 45.1784856043916 12.59837747015449 3 19.04048135846301 28.648342619043614 35.897342019679265 16.41383400281411 4 18.563837955198725 36.45260583614236 29.29508686081446 15.688469347844453 5 18.999215802804788 37.56054058861026 29.40546807489325 14.03477553369171 6 16.12882412338667 38.02966709192654 28.845169453339143 16.99633933134765 7 14.619233174171658 16.73238418197707 49.849123613022236 18.79925903082903 8 17.442299145992564 24.25660248699451 35.845100343824214 22.455998023188712 9 19.503540464209127 23.14461266500131 35.42509290059838 21.926753970191186 10-11 20.706282713876263 35.981329386173506 25.236374369677968 18.076013530272267 12-13 18.54803858213273 30.03598133751141 33.63066447379071 17.785315606565153 14-15 18.476030654898356 29.240018639141002 34.736995225997106 17.546955479963533 16-17 19.44198765314116 28.460944746807566 32.6005857870485 19.496481813002767 18-19 20.76808136225154 30.138722548449415 29.38384098144463 19.709355107854417 20-21 19.781810001374566 30.887956063460486 30.442634456843905 18.887599478321047 22-23 19.426082832389678 30.772250725995516 32.66948054776414 17.132185893850668 24-25 18.876746624099074 30.13634722856862 32.615132377883995 18.371773769448307 26-27 19.333649957253872 30.857884760109755 30.283230650082356 19.525234632554017 28-29 20.112328188737532 30.779649770807694 29.35011389574642 19.757908144708352 30-31 19.035296363110255 30.11933005949073 31.45579862789043 19.389574949508585 32-33 19.66856382007393 30.41465886234807 30.97356789430122 18.943209423276784 34-35 17.997801293344843 32.89943061491867 29.6582077535628 19.444560338173687 36-37 19.249754107663932 30.482060582152844 30.54469054912697 19.723494761056255 38-39 20.052662992211076 29.022376129794146 31.90662328050195 19.01833759749283 40-41 20.580519279722413 30.258222890700498 30.36188681336162 18.799371016215467 42-43 20.553564571284006 30.993356488560835 30.106270439059706 18.34680850109545 44-45 19.461082254673514 30.67754007338352 30.590970760586 19.270406911356968 46-47 19.815880763436684 29.898202530503916 30.579505733662433 19.706410972396966 48-49 20.16102420822105 29.678215490246266 30.65712043471054 19.50363986682214 50-51 19.239529559474775 30.383271968924824 30.82362677673478 19.55357169486562 52-53 19.016965889926592 30.70792897338861 31.535390469089986 18.739714667594814 54-55 18.889816720157278 30.75104067819038 31.772611562910807 18.586531038741537 56-57 20.07995451238614 29.50776476526245 30.43870719189108 19.973573530460328 58-59 19.617773326164524 29.95369359334921 30.366494708374685 20.062038372111576 60-61 20.291827106238006 29.557368538359274 31.09987813829974 19.050926217102983 62-63 17.84287237046694 32.63590769354736 30.35838855479618 19.162831381189523 64-65 17.93882089017325 31.466122115261324 30.968370012791635 19.626686981773794 66-67 19.15093791292687 29.258071694814074 31.565654650234805 20.02533574202425 68-69 20.334233199910887 30.385003678548962 30.08825960172885 19.1925035198113 70-71 19.27282913595161 32.13038916581865 29.16117829684631 19.435603401383432 72-73 18.815174027325696 31.621903439092456 30.6232368554778 18.939685678104045 74-75 19.12196886279943 29.481608633827978 31.87832938345426 19.51809311991833 76-77 20.014256462921715 30.565776728751448 29.831597983700686 19.588368824626144 78-79 19.748196302913186 32.0254435410362 28.50919604769023 19.717164108360375 80-81 19.630375177744558 31.795704962263464 30.442729427206768 18.131190432785214 82-83 18.99061289969738 31.4949604404419 30.40979472782295 19.104631932037773 84-85 19.492018084369416 31.683693440770043 29.69890618733365 19.12538228752689 86-87 20.703510361079935 31.92842896882744 28.063731601785836 19.304329068306796 88-89 19.738117183796987 31.60581470813432 29.751402632442485 18.90466547562621 90-91 19.96319935340582 31.35341964559813 29.936955273567932 18.746425727428115 92-93 19.441833652601794 31.56153235530868 29.994145252046174 19.00248874004335 94-95 19.591817552047253 31.965820541801875 29.254043096219057 19.188318809931822 96-97 20.188897661104683 30.349907911179724 30.241774317038665 19.21942011067693 98-99 20.952667079884783 31.025011849637217 29.043615415466512 18.978705655011485 100-101 21.080231888285265 32.46479308710395 27.990216210303714 18.464758814307068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40332.0 1 28453.5 2 15448.5 3 13759.5 4 11997.5 5 10172.5 6 9208.0 7 8820.0 8 9035.0 9 9713.5 10 11456.5 11 13640.5 12 14829.5 13 16834.5 14 22508.0 15 128742.5 16 442017.0 17 477667.5 18 382812.5 19 374064.5 20 310908.5 21 292943.0 22 237084.5 23 307115.0 24 520005.5 25 633113.5 26 617071.0 27 684180.0 28 855185.0 29 1010045.5 30 1187920.0 31 1460930.0 32 1829379.0 33 2396092.5 34 3125029.0 35 3936202.0 36 4649551.0 37 4867602.5 38 4408653.5 39 3574941.0 40 2918905.0 41 2609835.0 42 2443828.5 43 2323217.0 44 2104258.0 45 1911534.0 46 1778617.5 47 1620328.0 48 1574993.0 49 1519671.5 50 1383957.5 51 1272545.5 52 1150004.5 53 972925.5 54 812840.0 55 691567.5 56 589076.0 57 484770.0 58 381445.0 59 292914.5 60 218476.5 61 158874.0 62 114040.0 63 81472.0 64 59253.0 65 43687.5 66 31508.5 67 23281.5 68 18158.0 69 14292.0 70 10683.0 71 7298.0 72 5152.0 73 3979.5 74 3087.0 75 2297.5 76 1680.0 77 1316.0 78 1074.5 79 790.0 80 565.5 81 435.5 82 339.0 83 271.5 84 222.5 85 171.0 86 151.5 87 148.0 88 123.0 89 83.5 90 73.0 91 75.5 92 71.0 93 77.0 94 80.0 95 70.0 96 79.0 97 100.5 98 124.5 99 174.0 100 618.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002234294673132315 2 0.00181135377547672 3 0.13605716994202793 4 0.3520735042111788 5 0.38765012578845665 6 0.3864090130163707 7 0.41097896233638387 8 0.21321909067707007 9 0.14696758668465382 10-11 0.1867925766580377 12-13 1.4584168884675684E-5 14-15 0.0015101906880081672 16-17 0.04893061581653116 18-19 0.0029788164946950085 20-21 6.198271775987166E-4 22-23 0.002656506362343676 24-25 0.005707514493017829 26-27 0.014328216720749624 28-29 0.02652641557589237 30-31 0.03127356254785431 32-33 0.024906843621249133 34-35 0.02641192985014766 36-37 0.030976774711051154 38-39 0.026023990957815293 40-41 0.018655339628832902 42-43 0.011299084843402486 44-45 0.004066066285047581 46-47 0.002263463010901666 48-49 0.002331279396215408 50-51 0.0010872497903525722 52-53 0.0011740255952163926 54-55 0.0011587122178874831 56-57 3.835636416669705E-4 58-59 1.429248550698217E-4 60-61 2.5522295548182448E-5 62-63 0.00114850329966821 64-65 0.12032741459146097 66-67 0.20944470776971597 68-69 0.19978123746672988 70-71 0.030682174499580706 72-73 3.0626754657818937E-5 74-75 0.0 76-77 2.1876253327013526E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.38549604404419E-5 88-89 0.0 90-91 3.3033142523790425E-4 92-93 1.0719364130236629E-4 94-95 0.0 96-97 2.916833776935137E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.85675E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.43934368258117 #Duplication Level Percentage of deduplicated Percentage of total 1 74.92420277118428 29.549613832361377 2 11.94181159688229 9.419544235241482 3 4.641832660394316 5.492125008309645 4 2.3817434728569684 3.757375975590016 5 1.4059697028599576 2.7725261159195194 6 0.9199321918612141 2.176891312769077 7 0.6426463730658265 1.7741885821595182 8 0.49916216741758745 1.574930261929948 9 0.37007604452742526 1.31360006779266 >10 2.0454773245698856 14.387592394212895 >50 0.13348257468399488 3.620835446549484 >100 0.07956436264439995 6.023736875537641 >500 0.007169858306551071 1.9491907341120083 >1k 0.0053927450425520955 4.2976645160176625 >5k 7.050070394772323E-4 1.9679216393743129 >10k+ 8.311466635286502E-4 9.922263002122813 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 539300 0.7865242279505598 No Hit CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC 232644 0.339291938600649 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 165802 0.2418084369416998 No Hit CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT 141050 0.20570970211835055 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 113192 0.165081124439421 No Hit TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC 101392 0.14787180515550372 No Hit CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT 98291 0.14334925438436577 No Hit GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA 95191 0.13882816203011633 No Hit AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT 86431 0.1260524300871404 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 77821 0.11349546067743463 No Hit GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA 73837 0.10768512779377987 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 73806 0.10763991687023734 No Hit CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT 73093 0.10660006562875998 No Hit TTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACAA 70514 0.10283880847340213 No Hit GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA 70006 0.10209793269406059 No Hit CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA 69485 0.10133809749516898 No Hit GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA 69370 0.10117037955299524 No Hit CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA 69064 0.10072410398512416 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.625150399241623E-5 1.4584168884675685E-6 0.0 1.604258577314325E-5 1.4584168884675685E-6 2 3.646042221168921E-5 1.4584168884675685E-6 0.0 6.708717686950815E-5 2.916833776935137E-6 3 7.729609508878113E-5 1.4584168884675685E-6 0.0 7.4379261311846E-5 2.916833776935137E-6 4 2.318882852663434E-4 1.4584168884675685E-6 0.0 8.75050133080541E-5 2.916833776935137E-6 5 2.6980712436650017E-4 1.4584168884675685E-6 0.0 9.771393152732708E-5 2.916833776935137E-6 6 3.1210121413205964E-4 2.916833776935137E-6 0.0 1.1959018485434061E-4 4.375250665402705E-6 7 3.471032194552813E-4 2.916833776935137E-6 0.0 1.38549604404419E-4 4.375250665402705E-6 8 3.9377255988624346E-4 2.916833776935137E-6 0.0 1.48758522623692E-4 4.375250665402705E-6 9 5.337805811791301E-4 2.916833776935137E-6 0.0 1.6042585773143252E-4 4.375250665402705E-6 10-11 8.31297626426514E-4 2.916833776935137E-6 0.0 1.7646844350457578E-4 4.375250665402705E-6 12-13 0.0012586137747475116 2.916833776935137E-6 0.0 1.9834469683158932E-4 4.375250665402705E-6 14-15 0.0017632260181572902 4.375250665402705E-6 0.0 2.0636598971816094E-4 4.375250665402705E-6 16-17 0.002604003354358843 4.375250665402705E-6 0.0 2.173041163816677E-4 4.375250665402705E-6 18-19 0.003273416706165457 5.10445910963649E-6 0.0 2.224085754913042E-4 5.833667553870274E-6 20-21 0.003973456812629891 7.292084442337843E-6 0.0 2.4209720348561636E-4 5.833667553870274E-6 22-23 0.004718707842636817 7.292084442337843E-6 0.0 2.479308710394866E-4 5.833667553870274E-6 24-25 0.0054778138330841865 7.292084442337843E-6 0.0 2.544937470375907E-4 5.833667553870274E-6 26-27 0.00623181536442192 7.292084442337843E-6 0.0 2.6907791592226636E-4 5.833667553870274E-6 28-29 0.007052904072629161 9.479709775039195E-6 0.0 2.7782841725307176E-4 7.292084442337843E-6 30-31 0.007856491778174792 1.020891821927298E-5 0.0 2.8949575236081234E-4 7.292084442337843E-6 32-33 0.008708936449484086 1.020891821927298E-5 0.0 2.982462536916178E-4 7.292084442337843E-6 34-35 0.009634301965216759 1.020891821927298E-5 0.0 3.1210121413205964E-4 8.75050133080541E-6 36-37 0.010556750647172495 1.020891821927298E-5 0.0 3.456448025668137E-4 8.75050133080541E-6 38-39 0.011593685054872935 1.1667335107740548E-5 0.0 3.507492616764502E-4 8.75050133080541E-6 40-41 0.012951471178036241 1.3125751996208116E-5 0.0 3.638750136726583E-4 8.75050133080541E-6 42-43 0.01465854814598753 1.3125751996208116E-5 0.0 3.813760163342691E-4 8.75050133080541E-6 44-45 0.01666241295074197 1.3125751996208116E-5 0.0 3.88668100776607E-4 8.75050133080541E-6 46-47 0.019276625223320087 1.3125751996208116E-5 0.0 4.061691034382178E-4 8.75050133080541E-6 48-49 0.022915375360046668 1.3125751996208116E-5 0.0 4.0908593721515293E-4 8.75050133080541E-6 50-51 0.02802202209501586 1.3125751996208116E-5 0.0 4.1710723010172457E-4 8.75050133080541E-6 52-53 0.03496116965034455 1.38549604404419E-5 0.0 4.2804535676523134E-4 8.75050133080541E-6 54-55 0.04421482480767128 1.4584168884675685E-5 0.0 4.3971269187297187E-4 8.75050133080541E-6 56-57 0.05707806176395523 1.604258577314325E-5 0.0 4.521092354249462E-4 8.75050133080541E-6 58-59 0.07432384147008422 1.6771794217377038E-5 0.0 4.63047362088453E-4 8.75050133080541E-6 60-61 0.09700149487731068 1.750100266161082E-5 0.0 4.7179786341925844E-4 9.479709775039195E-6 62-63 0.12778940460130528 1.9688627994312177E-5 0.0 4.892988660808692E-4 1.020891821927298E-5 64-65 0.16938564188573302 2.1876253327013526E-5 0.0 5.038830349655449E-4 1.020891821927298E-5 66-67 0.2237102125642615 2.6980712436650014E-5 0.0 5.585736682830788E-4 1.020891821927298E-5 68-69 0.29279396215408177 2.916833776935137E-5 0.0 5.67324169613884E-4 1.020891821927298E-5 70-71 0.38355634958252816 2.916833776935137E-5 0.0 5.826375469427936E-4 1.020891821927298E-5 72-73 0.4967105407080614 2.916833776935137E-5 0.0 6.132643016006125E-4 1.020891821927298E-5 74-75 0.6413512232471652 2.916833776935137E-5 0.0 6.409742224814963E-4 1.020891821927298E-5 76-77 0.8196266452765524 3.0626754657818937E-5 0.0 6.497247238123017E-4 1.1667335107740548E-5 78-79 1.0371378568563825 3.135596310205272E-5 0.0 6.59204433587341E-4 1.1667335107740548E-5 80-81 1.301250592481861 3.3543588434754076E-5 0.0 6.708717686950815E-4 1.1667335107740548E-5 82-83 1.6163524993619425 3.646042221168921E-5 0.0 6.847267291355233E-4 1.1667335107740548E-5 84-85 2.0194516352499363 3.791883910015678E-5 0.0 6.912896051336275E-4 1.1667335107740548E-5 86-87 2.5006220148029312 3.791883910015678E-5 0.0 6.934772304663288E-4 1.1667335107740548E-5 88-89 3.031684836110402 3.791883910015678E-5 0.0 7.007693149086666E-4 1.1667335107740548E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTAT 182880 0.0 34.090626 1 TATTATA 178130 0.0 30.821968 9 TTATATA 182365 0.0 30.583727 4 TTTTATA 208265 0.0 28.239767 2 TTTATAT 223890 0.0 25.568644 3 CGGCGAG 5500 0.0 24.001818 1 TATATAT 235185 0.0 23.657816 5 CACGGTC 32855 0.0 22.512167 1 CTGGAAT 217470 0.0 19.782042 1 GGTGTCC 131315 0.0 18.82111 3 GGAAAAC 366720 0.0 18.120903 1 TGTCCAA 141490 0.0 17.728508 5 GTGTCCA 141000 0.0 17.657074 4 TGGTGTC 139985 0.0 17.634993 2 CTGGTGT 150550 0.0 16.872269 1 CGGCGCT 8975 0.0 16.673326 1 GTCCAAG 153040 0.0 16.654415 6 CGCCAGT 9200 0.0 16.161951 1 TGATACT 178160 0.0 15.933473 20-21 TTGATAC 171755 0.0 15.849305 20-21 >>END_MODULE