FastQCFastQC Report
Fri 27 May 2016
SRR1233112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68567500
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC5180610.7555489116563969No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC2278390.3322842454515623No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG1577820.23011193349618989No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT1438060.20972909906296716No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1125350.16412294454369783No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT1025710.14959127866700697No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1002070.14614358114266962No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA923910.1347445947424071No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT886490.12928719874576147No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT783080.11420570970211835No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA771120.11246144310351114No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT768780.11212017355160973No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA725090.10574835016589493No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA693870.10119517264009917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT1782500.033.7914621
TATTATA1750800.030.7867859
TTATATA1793100.030.1443714
CGACGGC56900.029.3492931
TTTTATA2045900.027.8243622
CGGCGAG55550.026.294031
TTTATAT2192250.025.2782483
CGGCCAG124400.024.9361881
GACGGCC72850.023.7582652
TATATAT2343900.023.3892235
CACGGTC326450.022.5900841
CGGCGCT108600.020.2402081
ACGGCCA88050.019.3827483
CTGGAAT2178200.019.1538091
CCGGAAT54750.018.1620541
GGTGTCC1335550.018.139263
CGCCGGT10850.017.9786431
TTACGCG73750.017.3396576
TGTCCAA1442750.017.2165265
GTGTCCA1430050.017.1633154
Produced by FastQC (version 0.11.5)