##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233112_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68567500 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22005523024757 33.0 31.0 34.0 30.0 34.0 2 32.51179405695118 34.0 31.0 34.0 31.0 34.0 3 32.618117067123634 34.0 31.0 34.0 31.0 34.0 4 35.507553097312865 37.0 35.0 37.0 33.0 37.0 5 35.92960665038101 37.0 35.0 37.0 35.0 37.0 6 35.95155407445218 37.0 35.0 37.0 35.0 37.0 7 35.94266426514019 37.0 35.0 37.0 35.0 37.0 8 35.97195843511868 37.0 35.0 37.0 35.0 37.0 9 37.75626785284574 39.0 38.0 39.0 35.0 39.0 10-11 37.79408589346264 39.0 38.0 39.0 35.0 39.0 12-13 37.71655718817224 39.0 37.5 39.0 35.0 39.0 14-15 39.01105494585627 40.0 38.0 41.0 36.0 41.0 16-17 38.9225154409888 40.0 38.0 41.0 35.5 41.0 18-19 38.91403269041456 40.0 38.5 41.0 35.0 41.0 20-21 38.99093670470704 40.0 39.0 41.0 35.0 41.0 22-23 38.938160243555615 40.0 39.0 41.0 35.0 41.0 24-25 38.374538651693584 40.0 38.0 41.0 33.5 41.0 26-27 37.39683081634885 40.0 37.0 41.0 31.0 41.0 28-29 37.014327873992784 40.0 36.0 41.0 30.0 41.0 30-31 36.92642902978817 39.0 36.0 41.0 30.0 41.0 32-33 36.88606559229956 39.0 36.0 41.0 30.0 41.0 34-35 36.83859144638495 39.0 36.0 41.0 30.0 41.0 36-37 36.73319574142268 39.0 36.0 41.0 30.0 41.0 38-39 36.627131928391734 39.0 36.0 41.0 30.0 41.0 40-41 36.51718183541765 39.0 35.0 41.0 30.0 41.0 42-43 36.40031280125424 39.0 35.0 41.0 30.0 41.0 44-45 36.27457010974587 39.0 35.0 41.0 29.0 41.0 46-47 36.484388106610275 39.0 35.0 41.0 30.0 41.0 48-49 36.44979728734459 39.0 35.0 41.0 29.5 41.0 50-51 36.39148935720276 39.0 35.0 41.0 29.0 41.0 52-53 36.301828898530644 39.0 35.0 41.0 28.5 41.0 54-55 36.197503795529954 39.0 35.0 41.0 28.0 41.0 56-57 35.88183104240347 39.0 35.0 41.0 27.5 41.0 58-59 35.71633089291574 39.0 35.0 41.0 27.0 41.0 60-61 35.46724739854888 39.0 34.0 41.0 26.5 41.0 62-63 35.39960559302877 38.5 34.0 41.0 26.5 41.0 64-65 35.25702224085755 38.0 34.0 40.0 26.5 41.0 66-67 34.95018894519998 38.0 34.0 40.0 26.0 41.0 68-69 34.597534261858755 37.0 34.0 40.0 26.0 41.0 70-71 34.285128129215735 37.0 33.5 39.0 26.0 41.0 72-73 33.94937438290735 36.0 33.0 39.0 26.0 41.0 74-75 33.56136057169942 36.0 33.0 39.0 26.0 40.5 76-77 33.21018623254457 35.5 32.0 38.5 25.5 40.0 78-79 32.87173891420862 35.0 32.0 37.0 25.0 39.0 80-81 32.530111517847374 35.0 32.0 37.0 25.0 39.0 82-83 32.17525273635469 35.0 32.0 36.5 24.0 39.0 84-85 31.872425259780506 35.0 32.0 36.0 24.0 37.5 86-87 31.549601990739056 35.0 32.0 36.0 22.5 37.0 88-89 31.2503714077369 35.0 31.0 35.0 21.5 37.0 90-91 31.002389368140882 35.0 31.0 35.0 20.5 36.0 92-93 30.782138053742663 35.0 31.0 35.0 20.0 36.0 94-95 30.545840565865753 35.0 31.0 35.0 19.0 36.0 96-97 30.27137219528202 34.5 31.0 35.0 15.0 36.0 98-99 29.987617887483136 34.0 31.0 35.0 2.0 35.5 100-101 28.999743639479345 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3599887699033104 1101 2 0.27096224223276266 1101 3 0.24755369117352188 1101 4 0.7994561917531726 1101 5 0.3869343015228637 1101 6 0.294702730067975 1101 7 0.14197307102413248 1101 8 0.17377357539113092 1101 9 0.259545322917873 1101 10-11 0.22924898205510402 1101 12-13 0.2590239928375624 1101 14-15 0.28700219384448644 1101 16-17 0.2520447416134317 1101 18-19 0.27387782848094133 1101 20-21 0.3344603539322648 1101 22-23 0.2814171371241869 1101 24-25 0.3121611319549018 1101 26-27 0.35841724184165 1101 28-29 0.531810933674528 1101 30-31 0.4853991054726521 1101 32-33 0.4362823517972316 1101 34-35 0.4405752571550252 1101 36-37 0.3991900873679768 1101 38-39 0.32324517203561953 1101 40-41 0.1478097857439522 1101 42-43 0.26849412187206667 1101 44-45 0.4029121981261241 1101 46-47 0.3926567256787479 1101 48-49 0.46350689066799333 1101 50-51 0.4490605668711325 1101 52-53 0.4304655659447931 1101 54-55 0.3196781727993283 1101 56-57 0.4251128623603577 1101 58-59 0.428764617463365 1101 60-61 0.5552660647799641 1101 62-63 0.39428398305740586 1101 64-65 0.3298125409691224 1101 66-67 0.42498992760204146 1101 68-69 0.40054933440740115 1101 70-71 0.47303677633481556 1101 72-73 0.47128407427870656 1101 74-75 0.3806180585651333 1101 76-77 0.3833920990876081 1101 78-79 0.4210960220620166 1101 80-81 0.3944229346760011 1101 82-83 0.3961414658483591 1101 84-85 0.4119049075115413 1101 86-87 0.34825986361337513 1101 88-89 0.39675014373818485 1101 90-91 0.37474906964191845 1101 92-93 0.38143812396472043 1101 94-95 0.40039573872302014 1101 96-97 0.4410526709947966 1101 98-99 0.4319021481380929 1101 100-101 0.3115316707109983 1102 1 0.3291542060994388 1102 2 0.24790311099800988 1102 3 0.20839278636842096 1102 4 0.778923772314684 1102 5 0.3330576117958586 1102 6 0.24824344334646753 1102 7 0.12635237859454662 1102 8 0.12393861524024885 1102 9 0.12196398786091578 1102 10-11 0.15700134308339386 1102 12-13 0.15046014563461085 1102 14-15 0.16025000684305013 1102 16-17 0.20609491356135123 1102 18-19 0.22308599411208263 1102 20-21 0.2662313613561196 1102 22-23 0.26569793202713043 1102 24-25 0.2872755303756662 1102 26-27 0.18271566941375283 1102 28-29 0.3558695703938781 1102 30-31 0.357085842786212 1102 32-33 0.25020705746689487 1102 34-35 0.24825009697710243 1102 36-37 0.14665460125217322 1102 38-39 0.20004924676207025 1102 40-41 0.23593592797794116 1102 42-43 0.20202959103692564 1102 44-45 0.19907903155690576 1102 46-47 0.24333932671804348 1102 48-49 0.28378949955641986 1102 50-51 0.27098093103214893 1102 52-53 0.25125143565602315 1102 54-55 0.27264128218498485 1102 56-57 0.2808472342521213 1102 58-59 0.32396722373020026 1102 60-61 0.4326437379065382 1102 62-63 0.41225221534020307 1102 64-65 0.3792512284350593 1102 66-67 0.3314856258733272 1102 68-69 0.31481887916201856 1102 70-71 0.24472336094346048 1102 72-73 0.30197244058417283 1102 74-75 0.2619473771271075 1102 76-77 0.17830434522657157 1102 78-79 0.2874503175635681 1102 80-81 0.28680515198836076 1102 82-83 0.29041608628318727 1102 84-85 0.2889922766616948 1102 86-87 0.2297660767326768 1102 88-89 0.2275962419765314 1102 90-91 0.23555770905681328 1102 92-93 0.26072884165824917 1102 94-95 0.2744476857920297 1102 96-97 0.25480401397368624 1102 98-99 0.25719787359609114 1102 100-101 0.19555380668193223 1103 1 0.2982877713514611 1103 2 0.22643876801218 1103 3 0.19909293138618267 1103 4 0.7557072855673894 1103 5 0.3431328007706682 1103 6 0.20499314426400161 1103 7 0.0877991956620292 1103 8 0.10833374189660816 1103 9 0.1556439915013499 1103 10-11 0.18886450252662002 1103 12-13 0.20802664846600294 1103 14-15 0.2276521425197231 1103 16-17 0.23402980581191457 1103 18-19 0.28746370970452517 1103 20-21 0.2773967111662472 1103 22-23 0.25321696529524473 1103 24-25 0.2666010915947439 1103 26-27 0.25908002065129665 1103 28-29 0.39691534597621114 1103 30-31 0.3321302315745527 1103 32-33 0.2570686832241691 1103 34-35 0.20619841874953693 1103 36-37 0.17394032122035696 1103 38-39 0.2706142210785316 1103 40-41 0.23778266889825517 1103 42-43 0.08044197515477691 1103 44-45 -0.06843459805961061 1103 46-47 0.19516738399045153 1103 48-49 0.17507853713058807 1103 50-51 0.17626250795277798 1103 52-53 0.2241470183782397 1103 54-55 0.2935685416860707 1103 56-57 0.23404642841479983 1103 58-59 0.2694999745311506 1103 60-61 0.427328067533729 1103 62-63 0.2369870880447067 1103 64-65 0.22135896806695854 1103 66-67 0.2664773845027497 1103 68-69 0.20232607496119925 1103 70-71 0.19936883460878363 1103 72-73 0.24903625686452813 1103 74-75 0.2833781314004469 1103 76-77 0.1502397215036595 1103 78-79 0.2045197464285664 1103 80-81 0.2386115543445726 1103 82-83 0.23048747102095746 1103 84-85 0.22601555077032742 1103 86-87 0.2601754946592685 1103 88-89 0.2752755004683429 1103 90-91 0.2431580302871872 1103 92-93 0.2753289991074972 1103 94-95 0.27540000144893995 1103 96-97 0.19035628959197837 1103 98-99 0.22509223631719166 1103 100-101 0.09029520478747344 1104 1 0.20932777601878882 1104 2 0.16317203973819971 1104 3 0.14439683475525555 1104 4 0.6758864151432533 1104 5 0.2853206490934781 1104 6 0.18087134711649355 1104 7 0.08340287585304651 1104 8 0.07396133235153002 1104 9 0.07050658479584371 1104 10-11 0.08923792209749593 1104 12-13 0.08446648745878349 1104 14-15 0.1399646092889384 1104 16-17 0.1549965753513689 1104 18-19 0.1393472992466087 1104 20-21 0.07653447623722798 1104 22-23 0.15352913697672932 1104 24-25 -0.01581048425642706 1104 26-27 -0.11588458727100459 1104 28-29 0.06866773875825061 1104 30-31 -0.0735376133160841 1104 32-33 0.04585675549409984 1104 34-35 -0.022008067074047233 1104 36-37 0.06342784704018811 1104 38-39 -0.07447860420142405 1104 40-41 -0.11880374425978601 1104 42-43 -0.12960688352094252 1104 44-45 -0.034483062895489525 1104 46-47 -0.13203573917517986 1104 48-49 -0.09321549899418358 1104 50-51 0.04201057905353167 1104 52-53 0.05261785652738382 1104 54-55 0.09379739512205276 1104 56-57 0.0701514603485478 1104 58-59 0.14387633063399363 1104 60-61 0.19535372492776304 1104 62-63 0.12804556463879635 1104 64-65 -0.016688327408616033 1104 66-67 0.0933468043448471 1104 68-69 0.0018070479971115105 1104 70-71 0.07551226311971249 1104 72-73 -0.0773287090576531 1104 74-75 0.06396171867059763 1104 76-77 0.05150122554504577 1104 78-79 -0.08742667849951147 1104 80-81 -0.014448747148335883 1104 82-83 -0.061553091009955097 1104 84-85 -0.03762375583175981 1104 86-87 -0.0026924259482470347 1104 88-89 0.12199588763755287 1104 90-91 0.0880677009934665 1104 92-93 0.037489504066812174 1104 94-95 0.09933065259980012 1104 96-97 0.0812270154982464 1104 98-99 -0.05828826780686214 1104 100-101 -0.026956088885317087 1105 1 0.20730454772266427 1105 2 0.1566651361874989 1105 3 0.144475394209735 1105 4 0.6950319461959893 1105 5 0.29952308868028155 1105 6 0.1550239381965639 1105 7 0.05081398417847538 1105 8 0.0713713696872702 1105 9 0.1033491400559754 1105 10-11 0.07646082008237443 1105 12-13 0.10014288398090798 1105 14-15 0.09194545421448908 1105 16-17 0.15446164323325462 1105 18-19 0.15653563527820324 1105 20-21 0.14988294897025867 1105 22-23 0.11897279874734323 1105 24-25 0.11597898645548099 1105 26-27 0.09668227855627975 1105 28-29 0.21763168903611074 1105 30-31 0.20555601149170855 1105 32-33 0.15490986485859537 1105 34-35 0.11968474185530198 1105 36-37 0.037804916566123836 1105 38-39 0.03810984963367048 1105 40-41 -0.0026115266954249705 1105 42-43 -0.1095879630608394 1105 44-45 -0.09180269563617571 1105 46-47 0.061560790812862365 1105 48-49 -0.09389597276225459 1105 50-51 -0.028145927094797685 1105 52-53 -0.042180766022127614 1105 54-55 0.10550223792976965 1105 56-57 -0.06667979291194115 1105 58-59 -0.03528422499288553 1105 60-61 0.09945911693963438 1105 62-63 0.030463069122831143 1105 64-65 0.03918354069438834 1105 66-67 0.04508764187649206 1105 68-69 0.03248230458056156 1105 70-71 -0.1151435357649504 1105 72-73 -0.04121464852178747 1105 74-75 0.018508031932128688 1105 76-77 0.010336106005084389 1105 78-79 0.03366073888746257 1105 80-81 0.04481800078154663 1105 82-83 0.030985662947735193 1105 84-85 0.003378023830087784 1105 86-87 -0.02463981840896068 1105 88-89 0.00929657054103572 1105 90-91 -0.053499697511679045 1105 92-93 -0.015697329440300223 1105 94-95 -0.027772242224692434 1105 96-97 -0.05312452345249241 1105 98-99 -0.08007106093026195 1105 100-101 -0.10545825749187543 1106 1 0.11313698327254684 1106 2 0.11733472759198804 1106 3 0.10567910854012297 1106 4 0.6061114638019802 1106 5 0.2150776554946816 1106 6 0.12987888509034917 1106 7 0.09197703601465435 1106 8 0.066617217414219 1106 9 0.036431392563592624 1106 10-11 0.02169264093581802 1106 12-13 0.05858213593700157 1106 14-15 0.0941219993872977 1106 16-17 0.09561923379963133 1106 18-19 0.12435514647493306 1106 20-21 0.11573414718984054 1106 22-23 0.11228654705001162 1106 24-25 0.055423406131062336 1106 26-27 0.019808501119143784 1106 28-29 0.17541712240287666 1106 30-31 0.07654968138650986 1106 32-33 0.04951693109764932 1106 34-35 -7.553249785914318E-4 1106 36-37 -0.0022001259087502945 1106 38-39 -0.04610110039673998 1106 40-41 -0.10360946475394428 1106 42-43 -0.04272936385508075 1106 44-45 -0.014826667248442504 1106 46-47 0.0394479053590544 1106 48-49 0.024658453841183814 1106 50-51 0.056857641852040786 1106 52-53 -0.0013000361951398531 1106 54-55 0.05861475867751409 1106 56-57 0.00840418886431138 1106 58-59 0.016242211536116713 1106 60-61 0.11156991865887278 1106 62-63 0.05838275462490117 1106 64-65 0.07110459456625051 1106 66-67 0.08277888484805374 1106 68-69 0.07038108617611272 1106 70-71 -0.04427389179446806 1106 72-73 -0.0049370726801925 1106 74-75 0.037111016955421405 1106 76-77 0.05360455789348606 1106 78-79 0.041364492143458165 1106 80-81 0.002876843177197941 1106 82-83 0.03561173223689451 1106 84-85 0.06645896218806868 1106 86-87 0.1076559936547774 1106 88-89 0.057348374030897986 1106 90-91 0.0767132561702546 1106 92-93 0.07997225014865705 1106 94-95 0.08258612047351832 1106 96-97 0.06871662171929316 1106 98-99 0.04948989322902264 1106 100-101 -0.05977329683825161 1107 1 0.07809471408605617 1107 2 0.07057109965455766 1107 3 0.0841285676201764 1107 4 0.6301411857905705 1107 5 0.2245981463384652 1107 6 0.07827342815415506 1107 7 0.029761762178914353 1107 8 0.014370540881863292 1107 9 0.008917567054858466 1107 10-11 0.013843876209634232 1107 12-13 0.026631129176649893 1107 14-15 0.031058133208937022 1107 16-17 0.08008648179939826 1107 18-19 0.09056341058595763 1107 20-21 0.054926014186641225 1107 22-23 0.03780220257865352 1107 24-25 0.012707763579932418 1107 26-27 -0.00528987112782886 1107 28-29 0.12945593026782376 1107 30-31 0.04336657082345141 1107 32-33 0.014505744491955852 1107 34-35 -0.0820130959658627 1107 36-37 -0.09731023009251771 1107 38-39 -0.06886877071253394 1107 40-41 -0.11154839628245128 1107 42-43 -0.09878797858095822 1107 44-45 -0.14817166786130542 1107 46-47 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2216 72-73 -1.022978752232774 2216 74-75 -1.1126688787546968 2216 76-77 -1.0645881864428972 2216 78-79 -1.0896146953031831 2216 80-81 -1.1689044950843126 2216 82-83 -1.1142348490143554 2216 84-85 -1.0574813040862203 2216 86-87 -1.0529410339734078 2216 88-89 -1.0307347200972394 2216 90-91 -1.0736424253249233 2216 92-93 -1.1000334973971064 2216 94-95 -1.1058494227345328 2216 96-97 -1.1589952323533268 2216 98-99 -1.10843727217609 2216 100-101 -0.9073641294236303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5057.0 3 2401.0 4 3750.0 5 7044.0 6 17048.0 7 43748.0 8 88337.0 9 125249.0 10 135998.0 11 142383.0 12 160415.0 13 183399.0 14 206567.0 15 227902.0 16 243579.0 17 253409.0 18 261356.0 19 272904.0 20 288052.0 21 314402.0 22 349550.0 23 403851.0 24 481456.0 25 583267.0 26 712782.0 27 868141.0 28 1056601.0 29 1275868.0 30 1544078.0 31 1879413.0 32 2307145.0 33 2873227.0 34 3658485.0 35 4828867.0 36 6880832.0 37 1.1391505E7 38 1.7593614E7 39 6864251.0 40 31567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.646125249100272 17.700173094389314 20.72147382895547 34.93222782755495 2 19.056737745799254 23.484040617694678 44.38162767974817 13.077593956757894 3 19.203533744120758 28.974744594742408 35.06273088562366 16.75899077551318 4 18.686835599956247 36.527421883545415 28.900255952163928 15.885486564334414 5 19.256283224559738 37.46762824953513 29.004948408502568 14.27114011740256 6 16.114176943945925 38.225726359000305 28.80223695547597 16.857859741577805 7 14.626081967370661 16.67088320038034 49.8842914212219 18.818743411027093 8 17.514455447300488 24.123887745073024 35.853212342932096 22.508444464694392 9 19.51071134283735 22.991230539249642 35.43693003244977 22.06112808546323 10-11 20.78727385423123 36.00656360520655 25.03305574798556 18.173106792576657 12-13 18.696087067488243 29.813173150545087 33.63976227804718 17.850977503919495 14-15 18.52251941517483 29.118877748204326 34.67653698909833 17.682065847522516 16-17 19.575640876274754 28.365065814412144 32.55270558961078 19.506587719702317 18-19 20.76949255843597 29.964579058900544 29.40741452055708 19.858513862106406 20-21 19.92894228315164 30.678077077332556 30.430405804499216 18.96257483501659 22-23 19.507899212549688 30.543684328171643 32.76828226645325 17.18013419282542 24-25 18.99204502680346 29.904836922127014 32.769122992529574 18.333995058539948 26-27 19.42617321620723 30.540535826249215 30.51236980791264 19.52092114963091 28-29 20.322761207634564 30.368732963768334 29.616260568115027 19.692245260482068 30-31 19.04483548140824 29.77317465103042 31.787316866786664 19.394673000774677 32-33 19.731461685553626 30.10513609593484 31.354797387527533 18.808604830984002 34-35 18.01533722980082 32.54807407665984 30.049061934491178 19.38752675904816 36-37 19.276087696005444 30.133302165868507 30.98128963300077 19.60932050512528 38-39 20.032653518274508 28.727350429076843 32.26724414533641 18.972751907312237 40-41 20.54654557193848 29.92018674791076 30.812165609743474 18.72110207040728 42-43 20.533415238584226 30.660354157809 30.50850931896462 18.297721284642147 44-45 19.45144690378415 30.358577911148092 31.02743337184823 19.16254181321953 46-47 19.769276661391203 29.62556721263649 31.004581366348894 19.600574759623417 48-49 20.136197381561697 29.38313139272932 31.060453329878822 19.42021789583016 50-51 19.26178083690365 30.009701166927087 31.250370713997455 19.47814728217181 52-53 19.03563976491418 30.38895802728252 31.897048196478195 18.67835401132511 54-55 18.89255378899945 30.467146158811286 32.11763422225078 18.522665829938482 56-57 20.006054698634014 29.223592706143265 30.84783982737112 19.9225127678516 58-59 19.628210098800306 29.567834226825894 30.783261449751016 20.02069422462278 60-61 20.27639705912047 29.20568712498703 31.448475319633275 19.069440496259226 62-63 17.870957693437038 32.326914960687944 30.671730380673218 19.130396965201797 64-65 17.938901906660366 31.167855524063583 31.35590778104287 19.537334788233185 66-67 19.122684786021054 28.979021892434105 31.966138882470325 19.932154439074516 68-69 20.266924178017835 30.10693419623729 30.451253976133984 19.174887649610895 70-71 19.27114087765851 31.829489346474983 29.51478350804622 19.38458626782029 72-73 18.743317061980022 31.375468362595687 30.955009407905383 18.926205167518905 74-75 19.004290131676242 29.27317737220976 32.244197106873806 19.478335389240193 76-77 19.871197174733705 30.326799665720294 30.237997639962455 19.56400551958355 78-79 19.626906077593823 31.802126629524018 28.837589001211 19.733378291671155 80-81 19.534704074391087 31.55995911500908 30.759532796172316 18.14580401442751 82-83 18.860920448393625 31.284490504021807 30.742847331126637 19.11174171645793 84-85 19.309236434591867 31.46007893412771 30.047825260001552 19.18285937127887 86-87 20.43652883469658 31.705986267721187 28.463135697324116 19.394349200258116 88-89 19.372438108433297 31.446268275786633 30.08612608014001 19.095167535640062 90-91 19.601974345992 31.243486656478787 30.199142917952663 18.955396079576545 92-93 19.011785466875704 31.50065920443359 30.234204980493672 19.25335034819703 94-95 19.080638273679682 31.868683061702274 29.53053906375316 19.52013960086489 96-97 19.548315893098042 30.343290188500383 30.438728989681703 19.669664928719875 98-99 20.163095783907767 31.065957356852113 29.186526112028677 19.584420747211446 100-101 20.167348962700988 32.4612841360703 28.15266999671856 19.218696904510153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12927.0 1 9180.0 2 4897.5 3 4790.0 4 5293.0 5 6117.0 6 6845.0 7 6758.5 8 8830.5 9 9969.0 10 22819.0 11 34692.0 12 26905.5 13 26768.5 14 31261.5 15 132476.5 16 436572.0 17 480730.5 18 399274.0 19 387276.5 20 321838.0 21 297529.5 22 236659.5 23 303844.5 24 518526.0 25 635215.5 26 615175.0 27 671599.0 28 847968.5 29 1006547.0 30 1177831.0 31 1447845.0 32 1812560.5 33 2379300.0 34 3119095.5 35 3951329.0 36 4683918.5 37 4911727.0 38 4431380.0 39 3566258.5 40 2893449.0 41 2583626.0 42 2436550.0 43 2316088.5 44 2092566.5 45 1914995.5 46 1789625.5 47 1628577.5 48 1582244.5 49 1529963.5 50 1396435.5 51 1285616.0 52 1163915.5 53 982170.0 54 816769.0 55 695900.5 56 591684.5 57 484498.0 58 377577.0 59 286196.5 60 210119.0 61 149224.0 62 105027.0 63 73638.0 64 52692.0 65 38751.5 66 27922.5 67 20450.0 68 15869.5 69 12410.0 70 8950.0 71 5600.5 72 3731.5 73 2920.5 74 2241.5 75 1513.5 76 1015.5 77 767.0 78 604.5 79 430.5 80 316.5 81 203.5 82 141.0 83 117.5 84 88.5 85 77.0 86 62.5 87 37.0 88 23.5 89 14.5 90 7.0 91 4.5 92 2.5 93 1.5 94 1.0 95 1.0 96 2.5 97 1.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0610814161227987 2 0.019596747730338718 3 0.0 4 0.0 5 0.0 6 0.008374229773580778 7 0.1442928501112043 8 0.0031662230648630916 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.885696576366355E-5 18-19 0.005574069347723047 20-21 0.0 22-23 8.320268348707478E-4 24-25 7.561891566704342E-4 26-27 0.0012148612680934845 28-29 5.833667553870274E-4 30-31 1.4219564662558792E-4 32-33 5.9065883982936524E-5 34-35 0.0017595799759361215 36-37 3.966893936631786E-4 38-39 5.10445910963649E-6 40-41 9.166150144018668E-4 42-43 6.818098953585883E-4 44-45 7.561891566704342E-4 46-47 5.84825172275495E-4 48-49 1.4948773106792577E-4 50-51 7.160826922375761E-4 52-53 1.669887337295366E-4 54-55 0.026190979691544828 56-57 0.3650556021438728 58-59 0.12059940934116017 60-61 0.3264870383199038 62-63 6.052430087140409E-5 64-65 1.793852772815109E-4 66-67 0.00173041163816677 68-69 6.205563860429503E-4 70-71 2.1584569949320012E-4 72-73 2.4063878659714877E-4 74-75 0.0012250701863127575 76-77 5.403434571772342E-4 78-79 4.594013198672841E-4 80-81 5.760746709446895E-5 82-83 0.0034148831443468115 84-85 1.0719364130236629E-4 86-87 2.916833776935137E-6 88-89 0.0 90-91 1.246946439639771E-4 92-93 0.0 94-95 2.4063878659714882E-5 96-97 0.0 98-99 1.4584168884675685E-6 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.85675E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.57720081348472 #Duplication Level Percentage of deduplicated Percentage of total 1 74.04112649504441 28.56299405255952 2 12.298419087724355 9.488771656710721 3 4.793208879998575 5.547257445140496 4 2.4769060599696022 3.822084098863384 5 1.5091138506442736 2.910869403335767 6 0.9912350597051207 2.2943444370966577 7 0.6859518896866358 1.8523472657781468 8 0.5077814357067062 1.5671029131693752 9 0.3769460319510827 1.3087370493380832 >10 2.0857998833596256 14.362911012782256 >50 0.13719374412970126 3.658509539715322 >100 0.08179519143625189 6.084537806229127 >500 0.00734187538887691 1.9481883035098655 >1k 0.005530422512467037 4.320023366898913 >5k 7.472927378411367E-4 2.0359856094121436 >10k+ 9.028000044930443E-4 10.235336039460195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 518061 0.7555489116563969 No Hit CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC 227839 0.3322842454515623 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 157782 0.23011193349618989 No Hit CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT 143806 0.20972909906296716 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 112535 0.16412294454369783 No Hit CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT 102571 0.14959127866700697 No Hit TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC 100207 0.14614358114266962 No Hit GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA 92391 0.1347445947424071 No Hit AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT 88649 0.12928719874576147 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 78308 0.11420570970211835 No Hit GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA 77112 0.11246144310351114 No Hit CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT 76878 0.11212017355160973 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 72509 0.10574835016589493 No Hit CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA 69387 0.10119517264009917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.20851715462865E-5 0.0 0.0 1.604258577314325E-5 0.0 2 4.958617420789733E-5 4.375250665402705E-6 0.0 5.979509242717031E-5 0.0 3 9.917234841579466E-5 5.833667553870274E-6 0.0 6.708717686950815E-5 1.4584168884675685E-6 4 2.683487074780326E-4 5.833667553870274E-6 0.0 1.1229810041200277E-4 1.4584168884675685E-6 5 3.0772596346665695E-4 5.833667553870274E-6 0.0 1.239654355197433E-4 1.4584168884675685E-6 6 3.6752105589382727E-4 5.833667553870274E-6 0.0 1.4146643818135413E-4 1.4584168884675685E-6 7 4.1419039632478944E-4 5.833667553870274E-6 0.0 1.4438327195828927E-4 1.4584168884675685E-6 8 4.681518211980895E-4 5.833667553870274E-6 0.0 1.618842746199001E-4 1.4584168884675685E-6 9 6.169103438217814E-4 5.833667553870274E-6 0.0 1.7792686039304334E-4 1.4584168884675685E-6 10-11 9.370328508404126E-4 5.833667553870274E-6 0.0 1.8376052794691363E-4 1.4584168884675685E-6 12-13 0.0013811207933787872 5.833667553870274E-6 0.0 1.976154883873555E-4 1.4584168884675685E-6 14-15 0.001885003828344332 5.833667553870274E-6 0.0 2.0636598971816094E-4 1.4584168884675685E-6 16-17 0.0027454697925401975 8.021292886571627E-6 0.0 2.216793670470704E-4 1.4584168884675685E-6 18-19 0.003441134648339228 9.479709775039195E-6 0.0 2.318882852663434E-4 2.1876253327013526E-6 20-21 0.004194406971232727 1.020891821927298E-5 0.0 2.595982061472272E-4 2.916833776935137E-6 22-23 0.004957159003901266 1.020891821927298E-5 0.0 2.6761949903379885E-4 2.916833776935137E-6 24-25 0.005741058081452583 1.020891821927298E-5 0.0 2.7637000036460425E-4 2.916833776935137E-6 26-27 0.006508914573230758 1.020891821927298E-5 0.0 2.8949575236081234E-4 2.916833776935137E-6 28-29 0.007370109745870858 1.020891821927298E-5 0.0 3.0407992124548806E-4 2.916833776935137E-6 30-31 0.008231304918510957 1.020891821927298E-5 0.0 3.179348816859299E-4 2.916833776935137E-6 32-33 0.00911875159514347 1.020891821927298E-5 0.0 3.31060633682138E-4 2.916833776935137E-6 34-35 0.010096620118860977 1.020891821927298E-5 0.0 3.646042221168921E-4 4.375250665402705E-6 36-37 0.011059904473693805 1.0938126663506763E-5 0.0 3.8429285011120425E-4 5.10445910963649E-6 38-39 0.01212454880227513 1.1667335107740548E-5 0.0 3.879388923323732E-4 5.833667553870274E-6 40-41 0.01348306413388267 1.1667335107740548E-5 0.0 3.9887701899587997E-4 5.833667553870274E-6 42-43 0.015190141101833959 1.1667335107740548E-5 0.0 4.149196047690232E-4 5.833667553870274E-6 44-45 0.017232653954132787 1.1667335107740548E-5 0.0 4.302329820979327E-4 5.833667553870274E-6 46-47 0.01986728406314945 1.1667335107740548E-5 0.0 4.4554635942684214E-4 5.833667553870274E-6 48-49 0.02355124512341853 1.1667335107740548E-5 0.0 4.564844860903489E-4 5.833667553870274E-6 50-51 0.028661537900608887 1.1667335107740548E-5 0.0 4.7471469719619357E-4 5.833667553870274E-6 52-53 0.035597768622160644 1.1667335107740548E-5 0.0 4.856528238597003E-4 6.562875998104058E-6 54-55 0.04486746636526051 1.2396543551974333E-5 0.0 4.973201589674409E-4 7.292084442337843E-6 56-57 0.057794873664637036 1.3125751996208116E-5 0.0 5.111751194078827E-4 7.292084442337843E-6 58-59 0.07511794946585482 1.3125751996208116E-5 0.0 5.221132460713894E-4 7.292084442337843E-6 60-61 0.09795311189703576 1.4584168884675685E-5 0.0 5.294053305137273E-4 8.75050133080541E-6 62-63 0.12906989462937982 1.6771794217377038E-5 0.0 5.461771247311045E-4 8.75050133080541E-6 64-65 0.17102271484303788 1.9688627994312177E-5 0.0 5.724286287235206E-4 8.75050133080541E-6 66-67 0.2258664819338608 2.5522295548182448E-5 1.4584168884675685E-6 6.198271775987166E-4 8.75050133080541E-6 68-69 0.2955263061946257 2.8439129325117587E-5 1.4584168884675685E-6 6.351405549276261E-4 8.75050133080541E-6 70-71 0.3867233018558355 2.916833776935137E-5 1.4584168884675685E-6 6.460786815911327E-4 8.75050133080541E-6 72-73 0.5003864804754439 3.135596310205272E-5 1.4584168884675685E-6 6.876435629124586E-4 8.75050133080541E-6 74-75 0.6456695956539176 3.20851715462865E-5 1.4584168884675685E-6 7.080613993510045E-4 8.75050133080541E-6 76-77 0.8248273599008276 3.20851715462865E-5 1.4584168884675685E-6 7.292084442337842E-4 8.75050133080541E-6 78-79 1.0434644693185549 3.20851715462865E-5 1.4584168884675685E-6 7.379589455645897E-4 8.75050133080541E-6 80-81 1.309154482808911 3.3543588434754076E-5 1.4584168884675685E-6 7.525431144492653E-4 8.75050133080541E-6 82-83 1.6260188864987057 3.500200532322164E-5 1.4584168884675685E-6 7.627520326685383E-4 8.75050133080541E-6 84-85 2.0316578553979654 3.500200532322164E-5 1.4584168884675685E-6 7.715025339993437E-4 8.75050133080541E-6 86-87 2.516442192000583 3.791883910015678E-5 1.4584168884675685E-6 7.780654099974478E-4 8.75050133080541E-6 88-89 3.051843803551245 4.010646443285814E-5 1.4584168884675685E-6 7.860867028840194E-4 8.75050133080541E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTAT 178250 0.0 33.791462 1 TATTATA 175080 0.0 30.786785 9 TTATATA 179310 0.0 30.144371 4 CGACGGC 5690 0.0 29.349293 1 TTTTATA 204590 0.0 27.824362 2 CGGCGAG 5555 0.0 26.29403 1 TTTATAT 219225 0.0 25.278248 3 CGGCCAG 12440 0.0 24.936188 1 GACGGCC 7285 0.0 23.758265 2 TATATAT 234390 0.0 23.389223 5 CACGGTC 32645 0.0 22.590084 1 CGGCGCT 10860 0.0 20.240208 1 ACGGCCA 8805 0.0 19.382748 3 CTGGAAT 217820 0.0 19.153809 1 CCGGAAT 5475 0.0 18.162054 1 GGTGTCC 133555 0.0 18.13926 3 CGCCGGT 1085 0.0 17.978643 1 TTACGCG 7375 0.0 17.339657 6 TGTCCAA 144275 0.0 17.216526 5 GTGTCCA 143005 0.0 17.163315 4 >>END_MODULE