FastQCFastQC Report
Fri 27 May 2016
SRR1233111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40085961
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC504930.12596180493215567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG152850.031.8115561
CGACGGC45050.028.0829661
ACGACGG37900.024.398921
GACGGCC71950.023.4444982
CGGCGAG28550.021.1777231
CACGGTC163850.020.7762341
TCACGGT152600.020.3765432
ACGGCCA94850.017.9396673
GGCCAGT299850.016.148142
CTGGAAT838850.015.281691
ACGGTCT230950.014.9377582
GCGACGG16150.014.4718391
CGACGGA17600.014.1456161
TTACGCG13800.014.0948358
CGGACGT16500.013.8569281
AGTGAAT438900.013.7852146
TAATACG441850.013.4692319
GCCAGTG367050.013.3620683
CGGTCTT263600.013.2520433
TACGCGC14800.013.12358959
Produced by FastQC (version 0.11.5)