##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233111_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40085961 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.429623428511544 31.0 27.0 33.0 10.0 34.0 2 26.73747499779287 31.0 27.0 34.0 2.0 34.0 3 27.230586888012986 31.0 27.0 34.0 10.0 34.0 4 30.60793258767078 35.0 30.0 37.0 10.0 37.0 5 30.806997567053465 35.0 30.0 37.0 12.0 37.0 6 30.935103289652954 35.0 31.0 37.0 13.0 37.0 7 30.375990561882748 35.0 31.0 37.0 2.0 37.0 8 30.561473155152747 35.0 31.0 37.0 8.0 37.0 9 31.839388558004135 37.0 31.0 39.0 10.0 39.0 10-11 31.944117355699667 37.0 30.5 39.0 11.0 39.0 12-13 31.981958808471624 37.0 31.0 39.0 10.5 39.0 14-15 32.63933993000691 38.0 31.0 40.0 10.0 41.0 16-17 32.84660808056965 38.0 31.0 40.0 10.5 41.0 18-19 32.794283315298344 38.0 31.0 40.0 10.0 41.0 20-21 32.763884954635365 38.0 31.0 40.0 10.0 41.0 22-23 32.67533749533908 38.0 31.0 40.0 10.0 41.0 24-25 32.51552361436464 38.0 30.5 40.0 9.0 41.0 26-27 32.37695123487248 38.0 30.0 40.0 8.5 41.0 28-29 32.32431878083202 38.0 30.0 40.0 8.0 41.0 30-31 32.16712115246533 37.0 30.0 40.0 8.0 41.0 32-33 31.89774131896202 37.0 30.0 40.0 7.0 41.0 34-35 31.66345187533361 37.0 30.0 40.0 2.0 41.0 36-37 31.585891067947706 37.0 30.0 40.0 2.0 41.0 38-39 31.447798108170588 37.0 30.0 40.0 2.0 41.0 40-41 31.301622156445244 37.0 30.0 40.0 2.0 41.0 42-43 31.10366070555225 37.0 29.0 40.0 2.0 41.0 44-45 31.21875331864939 37.0 29.5 40.0 2.0 41.0 46-47 31.184003983339704 37.0 29.5 40.0 2.0 41.0 48-49 31.1084233330467 36.5 29.0 40.0 2.0 41.0 50-51 30.767939653486167 36.0 29.0 39.5 2.0 40.5 52-53 30.765610957911175 36.0 29.0 39.5 2.0 40.5 54-55 30.962586190711505 37.0 29.0 40.0 2.0 41.0 56-57 30.947322156003693 36.5 29.0 40.0 2.0 41.0 58-59 30.79731970252628 36.0 29.0 40.0 2.0 41.0 60-61 30.541416195560338 36.0 28.0 40.0 2.0 41.0 62-63 30.292829751543188 36.0 28.0 40.0 2.0 41.0 64-65 30.037823216961172 35.0 28.0 39.0 2.0 41.0 66-67 29.771538831263143 35.0 28.0 39.0 2.0 41.0 68-69 29.39582058416911 35.0 28.0 39.0 2.0 40.5 70-71 29.024249150469412 34.5 27.5 38.5 2.0 40.0 72-73 28.56853441782274 34.0 26.0 37.5 2.0 40.0 74-75 28.14511215036107 34.0 26.0 37.0 2.0 39.5 76-77 27.741429462050316 34.0 26.0 36.5 2.0 39.0 78-79 27.296320375105886 34.0 25.0 36.0 2.0 39.0 80-81 26.831487013620553 33.0 24.0 36.0 2.0 38.0 82-83 26.59576536034648 33.0 24.0 35.0 2.0 37.0 84-85 26.31774901192964 33.0 23.5 35.0 2.0 37.0 86-87 25.96274474996371 33.0 21.5 35.0 2.0 36.0 88-89 25.617264233730108 33.0 20.0 35.0 2.0 36.0 90-91 25.310526745261264 32.0 18.5 35.0 2.0 36.0 92-93 25.024199581993308 32.0 17.0 35.0 2.0 35.0 94-95 24.72478953417133 32.0 8.5 35.0 2.0 35.0 96-97 24.39054555783258 32.0 2.0 35.0 2.0 35.0 98-99 23.94792980016121 32.0 2.0 35.0 2.0 35.0 100-101 22.335400029451705 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.838232570623525 1101 2 6.036278934176483 1101 3 5.381986885641641 1101 4 5.612715518585858 1101 5 5.349085724651001 1101 6 5.139002611175378 1101 7 6.376762777621096 1101 8 6.056890008659082 1101 9 6.259343550468625 1101 10-11 5.680720174767629 1101 12-13 5.498272116963481 1101 14-15 6.1508417071549175 1101 16-17 5.676152255417005 1101 18-19 5.678461868240603 1101 20-21 5.6355781388073645 1101 22-23 5.462258561382452 1101 24-25 5.602476914224283 1101 26-27 5.461687565257591 1101 28-29 5.360524776380743 1101 30-31 5.394507435806261 1101 32-33 5.625642305047631 1101 34-35 5.472578518991963 1101 36-37 5.432684990174259 1101 38-39 5.4576985356713905 1101 40-41 5.3771150425819165 1101 42-43 5.4297872656542445 1101 44-45 5.379954516458948 1101 46-47 5.113004342751708 1101 48-49 5.024693881612887 1101 50-51 4.965745598666178 1101 52-53 5.098865789801 1101 54-55 5.372801571291657 1101 56-57 5.279152110688113 1101 58-59 5.24557938238366 1101 60-61 5.197871949495902 1101 62-63 5.10333769624161 1101 64-65 5.183988611701007 1101 66-67 5.148922690105707 1101 68-69 5.198220939784438 1101 70-71 5.11026528771643 1101 72-73 5.219281567111235 1101 74-75 5.231333628361359 1101 76-77 5.141301490274177 1101 78-79 5.252668984182943 1101 80-81 5.340087508916621 1101 82-83 5.074795036752548 1101 84-85 5.101367718193785 1101 86-87 5.020282364836319 1101 88-89 4.97948385648618 1101 90-91 4.940596290814337 1101 92-93 4.900693251828372 1101 94-95 4.857338757260408 1101 96-97 4.764424018861678 1101 98-99 4.785460678096381 1101 100-101 4.416475759593457 1102 1 4.564246485219972 1102 2 5.819204278713972 1102 3 5.165664422331261 1102 4 5.352420399092779 1102 5 5.015819410711135 1102 6 4.994782372116781 1102 7 6.221266432392618 1102 8 5.947954467876251 1102 9 6.053481262583009 1102 10-11 5.51308666817355 1102 12-13 5.367156387995589 1102 14-15 6.027767678926068 1102 16-17 5.64602644344043 1102 18-19 5.546313464086516 1102 20-21 5.4392368550401535 1102 22-23 5.318438596565752 1102 24-25 5.432072249083767 1102 26-27 5.262957872359721 1102 28-29 5.154714053432599 1102 30-31 5.233743930044987 1102 32-33 5.4622569474256615 1102 34-35 5.372625013660642 1102 36-37 5.295322475242337 1102 38-39 5.4067519631086896 1102 40-41 5.278397107405876 1102 42-43 5.390008421444161 1102 44-45 5.270578438663716 1102 46-47 5.0065531149930536 1102 48-49 4.984313104088724 1102 50-51 4.851152958021064 1102 52-53 5.111720832255276 1102 54-55 5.367022972423808 1102 56-57 5.357455977442253 1102 58-59 5.209727854103427 1102 60-61 5.110543633426612 1102 62-63 5.064052348363084 1102 64-65 5.12438544273699 1102 66-67 5.123392288011253 1102 68-69 5.193885101120248 1102 70-71 5.117069379674405 1102 72-73 5.182011817648057 1102 74-75 5.229755323528597 1102 76-77 5.221403645290149 1102 78-79 5.1360067750187675 1102 80-81 5.1518697527102155 1102 82-83 5.084268826091552 1102 84-85 5.058107864835652 1102 86-87 4.959026163873325 1102 88-89 4.999641512547374 1102 90-91 4.898772448021102 1102 92-93 4.895904546854283 1102 94-95 4.958794726956107 1102 96-97 4.8643219532510145 1102 98-99 4.753457161887983 1102 100-101 4.3421968968600275 1103 1 4.365435435249996 1103 2 5.565989127288134 1103 3 4.997736043401126 1103 4 5.135282333069611 1103 5 4.901956478464189 1103 6 4.815888136356158 1103 7 6.05376797959056 1103 8 5.755777937058788 1103 9 5.922259459810885 1103 10-11 5.443686975123676 1103 12-13 5.324254788349176 1103 14-15 5.895214267230401 1103 16-17 5.370603348782215 1103 18-19 5.2019839073121545 1103 20-21 5.0898599439072605 1103 22-23 5.0259790655307235 1103 24-25 5.312247678682333 1103 26-27 5.222961028542315 1103 28-29 5.152881671072251 1103 30-31 5.174448592106835 1103 32-33 5.3790810050419005 1103 34-35 5.31327522165201 1103 36-37 5.214043447218948 1103 38-39 5.222300751724752 1103 40-41 5.05528396078012 1103 42-43 5.114395192149953 1103 44-45 5.031736764143428 1103 46-47 4.90368344885583 1103 48-49 4.947459068552224 1103 50-51 4.881263328238557 1103 52-53 5.0593156665489865 1103 54-55 5.209086498244364 1103 56-57 5.192557854680619 1103 58-59 5.145480695273271 1103 60-61 4.991092096138441 1103 62-63 4.877050485053573 1103 64-65 4.998845978563306 1103 66-67 5.0532869418410655 1103 68-69 5.066681563550208 1103 70-71 4.974485120974464 1103 72-73 5.025152008665312 1103 74-75 4.952761472940754 1103 76-77 4.993961581397478 1103 78-79 5.05853786829319 1103 80-81 5.14029414907208 1103 82-83 4.948534770225997 1103 84-85 4.953419352906209 1103 86-87 4.7884203249911295 1103 88-89 4.824512357652459 1103 90-91 4.859543598943787 1103 92-93 4.770704326521109 1103 94-95 4.7575047964898936 1103 96-97 4.773596056129453 1103 98-99 4.635152120499885 1103 100-101 4.203853509953877 1104 1 4.377155638044528 1104 2 5.653456211641231 1104 3 4.967166806147269 1104 4 5.106945184195077 1104 5 4.8669100914827474 1104 6 4.7347284598872506 1104 7 6.06866456244547 1104 8 5.844807673023595 1104 9 5.913679601409857 1104 10-11 5.343722635486138 1104 12-13 4.901865444520464 1104 14-15 5.714037647297346 1104 16-17 5.307680628761371 1104 18-19 5.191711434051541 1104 20-21 5.127743730524568 1104 22-23 5.093920375522895 1104 24-25 5.252178589810633 1104 26-27 5.035260498399865 1104 28-29 4.908359253511335 1104 30-31 4.945250461077713 1104 32-33 5.101031645391775 1104 34-35 5.178237693787668 1104 36-37 5.0807216710065575 1104 38-39 5.163325976433839 1104 40-41 4.927135746269133 1104 42-43 5.103448082625359 1104 44-45 4.865013405570306 1104 46-47 4.580835626388151 1104 48-49 4.590784012140585 1104 50-51 4.459280218419135 1104 52-53 4.535510901581038 1104 54-55 4.864937279850441 1104 56-57 4.892787811588587 1104 58-59 4.776280562727997 1104 60-61 4.625128962564997 1104 62-63 4.548636237699029 1104 64-65 4.622118690730144 1104 66-67 4.554428822437686 1104 68-69 4.78095028466868 1104 70-71 4.730451227345462 1104 72-73 4.777947356488426 1104 74-75 4.833685123201111 1104 76-77 4.8378935855153244 1104 78-79 4.809096706232172 1104 80-81 4.831060706890799 1104 82-83 4.840000487955582 1104 84-85 4.857618748372943 1104 86-87 4.733109690197701 1104 88-89 4.757510689110237 1104 90-91 4.713948277871989 1104 92-93 4.655296379319644 1104 94-95 4.6195812791485125 1104 96-97 4.55380733511922 1104 98-99 4.461677599256241 1104 100-101 4.170200658870936 1105 1 4.1817951458000735 1105 2 5.486888790221041 1105 3 4.758366899202571 1105 4 5.029244668516043 1105 5 3.6062242730977694 1105 6 4.188431708117157 1105 7 4.749090121888301 1105 8 5.224902636938026 1105 9 5.2720759597892375 1105 10-11 4.82555892649237 1105 12-13 4.433391826961266 1105 14-15 4.2918214426197565 1105 16-17 4.461646122261325 1105 18-19 4.808370417562472 1105 20-21 4.823404385776346 1105 22-23 4.6565943844514415 1105 24-25 4.937018651738526 1105 26-27 4.848542953425259 1105 28-29 4.733951191267607 1105 30-31 4.826129421574386 1105 32-33 4.955234839658072 1105 34-35 4.870747761704461 1105 36-37 4.798375018877593 1105 38-39 4.922336407136427 1105 40-41 4.695862865096121 1105 42-43 4.981018725333794 1105 44-45 4.725089845627021 1105 46-47 4.586113616882528 1105 48-49 4.576876143859376 1105 50-51 4.39975487489642 1105 52-53 4.571116457760507 1105 54-55 4.670255598931032 1105 56-57 4.622463984680504 1105 58-59 4.628989650922485 1105 60-61 4.6115781507720435 1105 62-63 4.530004969956984 1105 64-65 4.502250851772441 1105 66-67 4.564190448312019 1105 68-69 4.604122893097415 1105 70-71 4.593521208406749 1105 72-73 4.599578615145315 1105 74-75 4.656679902559265 1105 76-77 4.5418164681952256 1105 78-79 4.60381879965686 1105 80-81 4.6324750716026415 1105 82-83 4.5116718882051075 1105 84-85 4.495745367509308 1105 86-87 4.345508238426973 1105 88-89 4.315863795418405 1105 90-91 4.272815383924311 1105 92-93 4.242805830248841 1105 94-95 4.228841625402971 1105 96-97 4.08083557603905 1105 98-99 4.122105368324679 1105 100-101 3.734102015214731 1106 1 4.196822477074413 1106 2 5.374410467576354 1106 3 4.557454236198854 1106 4 4.812265483212947 1106 5 4.613710884751921 1106 6 4.430479422171089 1106 7 5.653574142799464 1106 8 5.0588968688641955 1106 9 5.254453937633304 1106 10-11 3.657464778275205 1106 12-13 4.6411792381769 1106 14-15 5.209981371215733 1106 16-17 4.822542149257256 1106 18-19 4.679946511829897 1106 20-21 4.775930004370412 1106 22-23 4.601930064159408 1106 24-25 4.821964277130213 1106 26-27 4.658157951536339 1106 28-29 4.621504573719392 1106 30-31 4.37428020986696 1106 32-33 4.608595224689726 1106 34-35 4.08284444715029 1106 36-37 4.3607071570188225 1106 38-39 4.644350614237588 1106 40-41 4.533140748653398 1106 42-43 4.488315399354544 1106 44-45 4.408779075783702 1106 46-47 4.237203295877656 1106 48-49 4.22351830085039 1106 50-51 4.027664010486948 1106 52-53 4.240604095792374 1106 54-55 4.40144145401484 1106 56-57 4.184716660459468 1106 58-59 4.226862803155029 1106 60-61 4.057136109441604 1106 62-63 4.095347016667901 1106 64-65 4.170137859896936 1106 66-67 4.2688927671863794 1106 68-69 4.245096514962501 1106 70-71 4.135144330519452 1106 72-73 4.2267199291980155 1106 74-75 4.357048447247454 1106 76-77 4.348741531765974 1106 78-79 4.450908575298801 1106 80-81 4.530233420987809 1106 82-83 4.350609727859023 1106 84-85 4.354936731995959 1106 86-87 4.269009589971191 1106 88-89 4.2417845943371155 1106 90-91 4.233302981898358 1106 92-93 4.201559349773515 1106 94-95 4.1554334199470375 1106 96-97 4.144871744717435 1106 98-99 4.061206245168766 1106 100-101 3.8476333195849186 1107 1 0.978078959075571 1107 2 1.7848057503692694 1107 3 0.9799502005933647 1107 4 1.44160643926271 1107 5 1.0344567417862294 1107 6 1.6298183353014295 1107 7 1.694781901944335 1107 8 1.6029333082976542 1107 9 2.420290425941001 1107 10-11 1.9710928154780376 1107 12-13 1.187199972513195 1107 14-15 2.159890054815115 1107 16-17 2.4349443535309767 1107 18-19 2.4176740052628176 1107 20-21 0.6473045254695471 1107 22-23 1.1540198789773903 1107 24-25 0.795709997834912 1107 26-27 0.986767016357657 1107 28-29 1.0547888910438985 1107 30-31 0.960704145569153 1107 32-33 1.4741198648652478 1107 34-35 0.640140721493637 1107 36-37 1.141744600611915 1107 38-39 0.682331611852419 1107 40-41 1.62853361083258 1107 42-43 1.6479408455857616 1107 44-45 1.0539356555939925 1107 46-47 2.0322210626752373 1107 48-49 2.3561582836758674 1107 50-51 2.3635775396673324 1107 52-53 1.4456469267726604 1107 54-55 1.4297546671157484 1107 56-57 1.6625186615777245 1107 58-59 1.5173670848357617 1107 60-61 1.387464028023647 1107 62-63 1.2265621168323655 1107 64-65 0.9250688502039068 1107 66-67 0.5687406908661785 1107 68-69 1.186181816581719 1107 70-71 1.2211908634197393 1107 72-73 1.873799609051808 1107 74-75 2.1804450767702335 1107 76-77 2.1692992333053382 1107 78-79 2.0310688608818204 1107 80-81 1.4641013290480878 1107 82-83 1.7263172640309499 1107 84-85 1.6251991288985614 1107 86-87 1.7712194746500707 1107 88-89 1.4567967799807242 1107 90-91 1.7772226332290266 1107 92-93 1.7613272259262587 1107 94-95 1.7934862289429745 1107 96-97 1.4592444751539482 1107 98-99 1.2084850467348005 1107 100-101 1.6216355738852286 1108 1 0.6001091010553878 1108 2 -0.08863676780252305 1108 3 0.2593888123486252 1108 4 -1.4959757361865371 1108 5 -0.4832084897517994 1108 6 -1.833665236041508 1108 7 -1.1868981915483445 1108 8 -1.4240393382904841 1108 9 -3.754672681490529 1108 10-11 -1.7137967613592267 1108 12-13 -0.796024942429117 1108 14-15 0.17040415804977016 1108 16-17 -1.916689983611061 1108 18-19 -1.5986002620494908 1108 20-21 -0.3156935183257801 1108 22-23 -0.668041415938827 1108 24-25 -2.6397512380119323 1108 26-27 -2.0332724429537947 1108 28-29 -1.8060959977324451 1108 30-31 -1.2012422150023099 1108 32-33 -1.7776571851511669 1108 34-35 -0.6408094635673471 1108 36-37 -0.09745251860368853 1108 38-39 -1.917914873152526 1108 40-41 -1.9563164984246804 1108 42-43 -1.43330555512539 1108 44-45 -0.9892866014994688 1108 46-47 -0.6735588898104332 1108 48-49 -1.5211980879912481 1108 50-51 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-1.10439790463899 2312 52-53 0.4762355840854475 2312 54-55 1.2991094565364207 2312 56-57 0.3130833550474179 2312 58-59 0.5915765041260919 2312 60-61 -1.5099017237189365 2312 62-63 -0.9719242035568527 2312 64-65 0.068813229315424 2312 66-67 -0.38625571316127605 2312 68-69 -0.8832147046583394 2312 70-71 -0.20690604102903976 2312 72-73 -0.716508418773774 2312 74-75 0.1219862924720907 2312 76-77 0.4517862368825085 2312 78-79 0.24581503953474027 2312 80-81 0.5558391860513261 2312 82-83 0.7339594622708674 2312 84-85 -0.7064983916166483 2312 86-87 -0.5948880900189835 2312 88-89 -0.22483944633109232 2312 90-91 0.04139644835311529 2312 92-93 0.4599995146551201 2312 94-95 0.16574665907915076 2312 96-97 -0.2669027829658077 2312 98-99 -0.40458108500904544 2312 100-101 -1.0113277122662332 2313 1 -4.393703082559984 2313 2 -22.55685325890314 2313 3 -12.837064447388894 2313 4 -9.864297636596842 2313 5 -6.81877630082764 2313 6 -6.128099002381028 2313 7 -25.955832081849834 2313 8 -13.710181761034104 2313 9 -10.1713905127293 2313 10-11 -7.551640391116624 2313 12-13 -6.41995354994059 2313 14-15 -6.606710733515968 2313 16-17 -7.847736626199062 2313 18-19 -9.250475853994523 2313 20-21 -7.223365429983101 2313 22-23 -8.362676986230138 2313 24-25 -11.315253555290482 2313 26-27 -10.142404797456646 2313 28-29 -8.188795705188738 2313 30-31 -7.681726150484902 2313 32-33 -7.857546558816807 2313 34-35 -7.7221696451622925 2313 36-37 -9.58341443330034 2313 38-39 -9.681167394048856 2313 40-41 -8.35988756228328 2313 42-43 -8.14884191905875 2313 44-45 -8.046821881156024 2313 46-47 -8.063289661512002 2313 48-49 -8.21426154402408 2313 50-51 -8.34274339477287 2313 52-53 -8.21703237323105 2313 54-55 -8.466115122523668 2313 56-57 -8.631995629373016 2313 58-59 -9.175919569211061 2313 60-61 -9.013027465821285 2313 62-63 -10.53277384320294 2313 64-65 -10.364077562769193 2313 66-67 -10.211323961787105 2313 68-69 -10.549841769913819 2313 70-71 -10.237406356907414 2313 72-73 -10.26571258442986 2313 74-75 -10.406013325013078 2313 76-77 -10.762351540076729 2313 78-79 -10.95103533066831 2313 80-81 -11.180211528121692 2313 82-83 -11.264487610484545 2313 84-85 -11.408176001827773 2313 86-87 -11.60392600124855 2313 88-89 -11.61914003759979 2313 90-91 -11.608906909661581 2313 92-93 -11.371919408560434 2313 94-95 -11.268286176847695 2313 96-97 -11.002758848063479 2313 98-99 -10.638132227219785 2313 100-101 -9.541654769193542 2314 1 -23.64762064830894 2314 2 -22.55685325890314 2314 3 -23.336710897059294 2314 4 -25.710534534919535 2314 5 -25.70030127971732 2314 6 -25.40905913064047 2314 7 -25.955832081849834 2314 8 -26.144957427647547 2314 9 -27.463240607389547 2314 10-11 -26.822970013215468 2314 12-13 -27.09171723367711 2314 14-15 -27.913276088681 2314 16-17 -28.745115791820286 2314 18-19 -27.834460412477686 2314 20-21 -27.15124829307506 2314 22-23 -27.006366502554812 2314 24-25 -27.625041415071344 2314 26-27 -27.433037795106465 2314 28-29 -28.081155053358728 2314 30-31 -27.898140205720082 2314 32-33 -27.578034994530622 2314 34-35 -27.694376027315986 2314 36-37 -27.609257821909175 2314 38-39 -26.84489518763839 2314 40-41 -26.59798015950386 2314 42-43 -26.85653876374169 2314 44-45 -27.013371487345676 2314 46-47 -26.361145595608523 2314 48-49 -27.003782384742888 2314 50-51 -26.471364233091606 2314 52-53 -26.143410569171614 2314 54-55 -25.859984107374782 2314 56-57 -25.74064445903408 2314 58-59 -25.641459187236517 2314 60-61 -25.411334521341768 2314 62-63 -25.18651266502642 2314 64-65 -24.91514117505268 2314 66-67 -24.608472749888147 2314 68-69 -24.236350209551908 2314 70-71 -24.058039420528697 2314 72-73 -23.65795963332602 2314 74-75 -23.249838859904454 2314 76-77 -22.901632826919297 2314 78-79 -22.535520758692275 2314 80-81 -22.065349350757394 2314 82-83 -21.86828674193834 2314 84-85 -21.617370539545444 2314 86-87 -21.265083714205915 2314 88-89 -20.91524273732366 2314 90-91 -20.607049961222245 2314 92-93 -20.351530091298756 2314 94-95 -20.05107312924132 2314 96-97 -19.707959904369854 2314 98-99 -19.2961484191275 2314 100-101 -17.841884574803107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2840525.0 3 164255.0 4 148771.0 5 147096.0 6 168256.0 7 165612.0 8 173380.0 9 189929.0 10 205147.0 11 228471.0 12 250054.0 13 272354.0 14 292324.0 15 320787.0 16 344588.0 17 356536.0 18 352960.0 19 362326.0 20 368011.0 21 355642.0 22 385079.0 23 431395.0 24 516688.0 25 636881.0 26 777626.0 27 897998.0 28 963230.0 29 1035094.0 30 1189224.0 31 1367150.0 32 1605497.0 33 1937554.0 34 2377601.0 35 2953574.0 36 3811876.0 37 5146935.0 38 5138557.0 39 1205027.0 40 1951.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.85589158367132 15.774545623998772 17.322914447053996 34.04664834527591 2 21.370582452217405 20.031891491942623 41.42809062724416 17.16943542859581 3 24.313917055805714 22.27923003731235 31.985658933818012 21.421193973063925 4 23.661052989335396 29.956704140749963 28.04002816938454 18.342214700530096 5 23.83700783238467 32.86662702876434 27.729857301260623 15.56650783759036 6 19.23469255757231 35.3756209371218 28.602006828566985 16.78767967673891 7 16.932799130698182 18.899220987775063 46.24202760374397 17.92595227778278 8 18.22017522235108 24.110565046841685 36.77372196223769 20.895537768569543 9 20.042963763554177 22.106184013573206 36.72755303068444 21.123299192188178 10-11 21.430020064500606 32.885132019108646 27.92683263482742 17.75801528156332 12-13 20.036096970055866 27.32506132558527 33.80206913908598 18.836772565272884 14-15 20.561743457829714 27.41227235484378 33.49565510029366 18.530329087032847 16-17 21.176742344739594 27.642762723656023 31.6806273355628 19.499867596041582 18-19 21.399635868914995 28.062349788741624 30.15192494697031 20.38608939537307 20-21 21.333341208225683 28.355019453206847 29.87126900778325 20.44037033078422 22-23 21.063842337796444 28.422644539071996 31.267947360176752 19.245565762954808 24-25 20.89733798350551 28.382002582813726 31.173496595883556 19.54716283779721 26-27 21.085175881669862 28.63602096924172 30.325235683083164 19.953567466005254 28-29 21.479190155391528 28.476835168545843 29.60404850184527 20.439926174217355 30-31 21.063739195770747 28.153543035185706 30.36530010052394 20.4174176685196 32-33 21.618950257271898 28.4156401577982 29.983900555282556 19.98150902964735 34-35 20.500116316000554 29.711927600362237 29.32148796149747 20.466468122139737 36-37 20.79669655427682 28.727460579075547 29.649747516787027 20.826095349860605 38-39 21.16747787061152 28.04384876314857 30.309669708514686 20.479003657725222 40-41 21.432377657537007 28.368834921920666 29.610204478905995 20.588582941636332 42-43 21.160425498316144 28.988027375304316 29.818908804128192 20.03263832225135 44-45 21.165690282051468 28.74149623263208 29.869425357993695 20.223388127322757 46-47 21.040920922712367 28.65990667656962 29.654891062996874 20.64428133772114 48-49 21.486772732678077 28.63153283766643 29.111008914398184 20.770685515257313 50-51 21.206292021929205 28.699726353722355 29.421038054118505 20.672943570229936 52-53 20.941911462523336 28.777307142041785 29.817457375379792 20.46332402005509 54-55 20.73669368809198 28.874748306908543 29.80691708524523 20.581640919754246 56-57 21.007939165057547 28.484875198150895 29.349897513278574 21.157288123512988 58-59 21.231320574647555 28.401185288385218 29.226765090475844 21.140729046491387 60-61 21.446324103536277 28.348942538770117 29.440888162097494 20.76384519559612 62-63 20.49898466947069 29.5458664443644 29.29571527548011 20.6594336106848 64-65 20.308429926292053 29.69700710652318 29.20492787449998 20.789635092684783 66-67 20.636785455367807 28.81200833740681 29.726640226186685 20.824565981038702 68-69 21.22081094675515 29.218082111091213 28.95994061362281 20.60116632853083 70-71 20.743414383903634 29.97098036392342 28.638286855590167 20.647318396582783 72-73 20.478598527160806 29.784733933351333 28.989903128850802 20.746764410637063 74-75 20.588654130031852 29.010766291462836 29.51342224450386 20.88715733400145 76-77 20.847076078873176 29.094904078106687 28.902900867112287 21.155118975907854 78-79 20.816436673257567 29.617450516648535 28.4317116301123 21.134401179981595 80-81 20.920890261079762 29.603631660918712 29.15444277323362 20.321035304767904 82-83 20.9769365007311 29.46693861801978 29.049437282305185 20.506687598943937 84-85 20.741048007279005 29.712402015001704 28.72189346394563 20.82465651377366 86-87 21.170231808478658 30.040648877778192 28.034529648406707 20.754589665336443 88-89 20.958868880676047 29.71514922050232 28.58016284455262 20.74581905426901 90-91 21.045713736345373 30.04966419825226 28.271617978846802 20.63300408655557 92-93 20.640173214200974 30.399480692897747 28.17674509585521 20.783600997046072 94-95 20.71611397483421 30.407429739903435 28.164060525400377 20.712395759861977 96-97 21.104371106880105 29.47437560159893 28.708112130318302 20.71314116120266 98-99 21.361790283099676 29.852885912835557 27.963064673242815 20.822259130821948 100-101 21.91608223134174 30.35652450970034 27.17866683055723 20.54872642840069 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 866.0 1 1015.5 2 1439.0 3 2079.0 4 3012.0 5 4046.5 6 4928.5 7 5791.0 8 7013.0 9 8807.5 10 11403.5 11 15543.5 12 19674.5 13 25640.0 14 31879.5 15 44504.5 16 82986.5 17 114339.0 18 139063.0 19 167028.0 20 190889.5 21 190953.5 22 175915.0 23 198435.0 24 253396.0 25 313854.0 26 370237.0 27 420578.5 28 482543.5 29 568080.0 30 671258.0 31 800339.5 32 961481.0 33 1162730.0 34 1385695.5 35 1618768.0 36 1825203.0 37 1933272.5 38 1869687.0 39 1707356.5 40 1586987.0 41 1514344.5 42 1456795.0 43 1412675.5 44 1372022.5 45 1338380.0 46 1314462.5 47 1291002.5 48 1264235.5 49 1211728.5 50 1134521.0 51 1061950.0 52 982966.5 53 879306.0 54 776435.0 55 685435.5 56 599417.5 57 511882.0 58 429470.5 59 350825.0 60 274773.0 61 212924.0 62 163559.0 63 122141.5 64 90398.5 65 66436.0 66 48052.0 67 35306.5 68 26382.5 69 19388.0 70 14303.5 71 10453.0 72 7830.5 73 6169.5 74 4856.5 75 3735.0 76 2885.0 77 2246.0 78 1783.5 79 1391.0 80 1063.0 81 847.0 82 681.5 83 550.5 84 431.0 85 299.5 86 237.5 87 195.5 88 134.5 89 91.5 90 72.5 91 53.0 92 39.5 93 27.5 94 21.5 95 19.0 96 14.0 97 8.0 98 7.0 99 4.5 100 3.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 4.175192407137252 2 9.267913023215286 3 4.87539016465141 4 3.2586745269746684 5 2.0007303803942733 6 2.226163419158144 7 7.563388588837873 8 6.314337830144573 9 5.112153853564843 10-11 1.7256877039819503 12-13 3.108659413204538 14-15 3.9160019139867948 16-17 3.4675182166644327 18-19 1.926788034344493 20-21 1.7969969586110208 22-23 2.160064217993926 24-25 2.2384507633482955 26-27 1.6876843740879757 28-29 1.4177794066107083 30-31 1.4430426153435614 32-33 1.9627307924587365 34-35 1.9439349352258262 36-37 1.8862426673517945 38-39 1.2125828790782887 40-41 1.765806487712743 42-43 2.7193660144507947 44-45 1.4720465351947032 46-47 0.6690634159924469 48-49 0.7536803221457008 50-51 0.7536803221457008 52-53 0.7500144501961672 54-55 0.3312593653423951 56-57 0.2471526128561568 58-59 0.9665141868496054 60-61 0.8402455413255528 62-63 0.0 64-65 0.03380859448523636 66-67 0.27915259409647186 68-69 0.0 70-71 0.0 72-73 0.24639923189068613 74-75 0.3312593653423951 76-77 0.24534399961123546 78-79 0.29745077085715865 80-81 0.05702619927210926 82-83 0.3635424881045012 84-85 0.06645718185476457 86-87 0.03309886970153965 88-89 0.06741013393691622 90-91 0.09981299936903097 92-93 0.06592831839555999 94-95 0.06589838272805784 96-97 0.0999714089428965 98-99 0.0664646657716401 100-101 0.06663055926238116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.0085961E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.55685659843672 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28419380280549 56.347931826378726 2 7.196569050463212 9.291757523830029 3 2.1496855771383294 4.163308306051404 4 1.008983758418994 2.6054727920962684 5 0.5602281652242264 1.8083284662392847 6 0.3592508480008958 1.391526328635637 7 0.24759503825473547 1.1188770165366786 8 0.18346477802371983 0.9475127492593044 9 0.13269148447547352 0.7709530621605154 >10 0.783033565492138 9.326806177252521 >50 0.058215685105233346 2.5781434248352544 >100 0.03176696422055625 3.8012591918572967 >500 0.002222009052036797 0.9946251082915251 >1k 0.001769748871778364 2.3165512325836453 >5k 2.000684340261681E-4 0.8865422617176714 >10k+ 1.294560190766668E-4 1.6504045322744638 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 50493 0.12596180493215567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005939735360217508 7.4839168755365504E-6 0.0 9.978555834048733E-6 0.0 2 0.005959692471885606 1.2473194792560917E-5 0.0 2.7441028543634016E-5 0.0 3 0.00598713350042924 1.2473194792560917E-5 0.0 2.9935667502146202E-5 2.4946389585121833E-6 4 0.0060869190587697275 1.2473194792560917E-5 0.0 3.741958437768275E-5 2.4946389585121833E-6 5 0.0061193493652303855 1.2473194792560917E-5 0.0 3.991422333619493E-5 2.4946389585121833E-6 6 0.006174231422317654 1.2473194792560917E-5 0.0 4.240886229470712E-5 2.4946389585121833E-6 7 0.006214145645653849 1.2473194792560917E-5 0.0 4.739814021173148E-5 2.4946389585121833E-6 8 0.006269027702741117 1.4967833751073101E-5 0.0 5.2387418128755854E-5 2.4946389585121833E-6 9 0.006401243567542262 1.7462472709585285E-5 0.0 5.488205708726803E-5 2.4946389585121833E-6 10-11 0.006703094881522237 2.245175062660965E-5 0.0 5.488205708726803E-5 2.4946389585121833E-6 12-13 0.00717458164468104 3.1182986981402294E-5 0.0 6.361329344206068E-5 2.4946389585121833E-6 14-15 0.0077283914934707446 3.2430306460658384E-5 0.0 8.606504406867031E-5 2.4946389585121833E-6 16-17 0.00845433143039779 3.2430306460658384E-5 0.0 9.729091938197516E-5 2.4946389585121833E-6 18-19 0.009322465787960029 3.492494541917057E-5 2.4946389585121833E-6 1.0851679469527998E-4 2.4946389585121833E-6 20-21 0.010271675911673914 3.866690385693884E-5 2.4946389585121833E-6 1.1600071157081654E-4 2.4946389585121833E-6 22-23 0.011233359230180361 4.365618177396321E-5 2.4946389585121833E-6 1.347105037596579E-4 2.4946389585121833E-6 24-25 0.012234956772023003 4.49035012532193E-5 2.4946389585121833E-6 1.4843101803147492E-4 2.4946389585121833E-6 26-27 0.013320124718975802 4.864545969098758E-5 2.4946389585121833E-6 1.621515323032919E-4 2.4946389585121833E-6 28-29 0.014501336265831322 5.2387418128755854E-5 2.4946389585121833E-6 1.6838812969957237E-4 2.4946389585121833E-6 30-31 0.01562766575559957 5.862401552503631E-5 2.4946389585121833E-6 1.796140050128772E-4 3.741958437768275E-6 32-33 0.016877479873814175 5.9871335004292403E-5 2.4946389585121833E-6 1.920871998054381E-4 4.989277917024367E-6 34-35 0.018069917295983 6.11186544835485E-5 2.4946389585121833E-6 2.083023530357673E-4 4.989277917024367E-6 36-37 0.01936588223493008 6.236597396280459E-5 2.4946389585121833E-6 2.2825946470386477E-4 4.989277917024367E-6 38-39 0.020635653464812782 6.486061292131677E-5 2.4946389585121833E-6 2.369907010586574E-4 6.2365973962804585E-6 40-41 0.02196903798813754 6.984989083834114E-5 2.4946389585121833E-6 2.4821657637196225E-4 7.4839168755365504E-6 42-43 0.02343463837626345 6.984989083834114E-5 2.4946389585121833E-6 2.6817368804005974E-4 8.731236354792642E-6 44-45 0.02499503504481282 7.109721031759723E-5 2.4946389585121833E-6 2.8813079970815717E-4 9.978555834048733E-6 46-47 0.026680163661287802 7.60864882346216E-5 2.4946389585121833E-6 3.043459529384864E-4 9.978555834048733E-6 48-49 0.02855987411652673 7.982844667238987E-5 2.4946389585121833E-6 3.13077189293279E-4 1.2473194792560917E-5 50-51 0.03072771537147382 8.357040511015813E-5 2.4946389585121833E-6 3.3053966200286434E-4 1.2473194792560917E-5 52-53 0.03303525640809759 8.98070025064386E-5 2.4946389585121833E-6 3.4800213471244956E-4 1.2473194792560917E-5 54-55 0.035597250618489604 9.479628042346297E-5 2.4946389585121833E-6 3.617226489842666E-4 1.2473194792560917E-5 56-57 0.03858957004922496 9.729091938197514E-5 2.4946389585121833E-6 3.704538853390592E-4 1.2473194792560917E-5 58-59 0.042191828705316556 9.978555834048734E-5 2.4946389585121833E-6 3.929056359656689E-4 1.372051427181701E-5 60-61 0.04647013451916495 1.0352751677825562E-4 2.4946389585121833E-6 4.053788307582298E-4 1.6215153230329192E-5 62-63 0.05213047031602909 1.0477483625751171E-4 2.4946389585121833E-6 4.253359424263273E-4 1.7462472709585285E-5 64-65 0.05942853658915649 1.0726947521602389E-4 2.4946389585121833E-6 4.5776624888698564E-4 1.9957111668097467E-5 66-67 0.06911397234558003 1.1350607261230435E-4 2.4946389585121833E-6 4.6774480472103436E-4 1.9957111668097467E-5 68-69 0.08220334296089346 1.1475339209156044E-4 2.4946389585121833E-6 4.814653189928514E-4 2.120443114735356E-5 70-71 0.10014229171155457 1.1724803105007261E-4 2.4946389585121833E-6 4.939385137854123E-4 2.245175062660965E-5 72-73 0.12447874207132019 1.1724803105007261E-4 2.4946389585121833E-6 5.02669750140205E-4 2.245175062660965E-5 74-75 0.15760505280140347 1.1974267000858481E-4 2.4946389585121833E-6 5.17637583891278E-4 2.245175062660965E-5 76-77 0.20225035892241677 1.209899894878409E-4 2.4946389585121833E-6 5.338527371216073E-4 2.369907010586574E-5 78-79 0.2622988132927635 1.2223730896709697E-4 3.741958437768275E-6 5.538098487897047E-4 2.7441028543634016E-5 80-81 0.3424640362245525 1.2473194792560918E-4 4.989277917024367E-6 5.700250020200339E-4 2.7441028543634016E-5 82-83 0.4478338438736694 1.2473194792560918E-4 4.989277917024367E-6 5.812508773333387E-4 2.7441028543634016E-5 84-85 0.5841720995537565 1.2597926740486525E-4 4.989277917024367E-6 5.949713916051558E-4 2.868834802289011E-5 86-87 0.7593793248464219 1.2722658688412136E-4 4.989277917024367E-6 6.199177811902776E-4 3.1182986981402294E-5 88-89 0.9722967599554367 1.2972122584263354E-4 4.989277917024367E-6 6.348856149413506E-4 3.2430306460658384E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCAG 15285 0.0 31.811556 1 CGACGGC 4505 0.0 28.082966 1 ACGACGG 3790 0.0 24.39892 1 GACGGCC 7195 0.0 23.444498 2 CGGCGAG 2855 0.0 21.177723 1 CACGGTC 16385 0.0 20.776234 1 TCACGGT 15260 0.0 20.376543 2 ACGGCCA 9485 0.0 17.939667 3 GGCCAGT 29985 0.0 16.14814 2 CTGGAAT 83885 0.0 15.28169 1 ACGGTCT 23095 0.0 14.937758 2 GCGACGG 1615 0.0 14.471839 1 CGACGGA 1760 0.0 14.145616 1 TTACGCG 1380 0.0 14.094835 8 CGGACGT 1650 0.0 13.856928 1 AGTGAAT 43890 0.0 13.785214 6 TAATACG 44185 0.0 13.469231 9 GCCAGTG 36705 0.0 13.362068 3 CGGTCTT 26360 0.0 13.252043 3 TACGCGC 1480 0.0 13.1235895 9 >>END_MODULE