FastQCFastQC Report
Fri 27 May 2016
SRR1233111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40085961
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC636260.15872389837429618No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT502510.12535810230419572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG139550.030.9370771
CGACGGC43650.024.3730011
ACGACGG37900.022.3062231
GACGGCC68100.020.6096362
CACGGTC192900.020.4357741
TCACGGT170400.019.6732252
CGGCGAG24500.019.579451
TTACGCG12150.017.950747
TACGCGC13750.016.5515778
ACGGCCA89850.015.7789173
CTGGAAT868000.015.3920061
CGGCGCT50600.014.8303641
ACGGTCT268900.014.6709522
CGGACGT12150.014.4634081
GGCCAGT310550.013.9553392
TAATACG486100.013.6353389
TATGGCG1088100.013.6300927
TGGCGAG1096100.013.3233539
ATGGCGA1119550.013.2641398
TGAATTG475700.013.2370352
Produced by FastQC (version 0.11.5)