##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233111_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40085961 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.776886401700587 31.0 30.0 34.0 27.0 34.0 2 31.085091012287318 31.0 31.0 34.0 28.0 34.0 3 30.903727616758395 31.0 31.0 34.0 27.0 34.0 4 33.1159123514589 35.0 33.0 37.0 25.0 37.0 5 33.82352579747309 35.0 33.0 37.0 28.0 37.0 6 34.35768677218441 35.0 35.0 37.0 30.0 37.0 7 34.761845150724966 35.0 35.0 37.0 32.0 37.0 8 34.82184935020019 35.0 35.0 37.0 32.0 37.0 9 36.3764515212695 38.0 35.0 39.0 32.0 39.0 10-11 36.270162015075556 38.0 35.0 39.0 32.0 39.0 12-13 36.215385194332754 38.0 35.0 39.0 32.0 39.0 14-15 37.39408987849886 39.0 36.0 41.0 32.0 41.0 16-17 37.39419521462888 39.0 36.0 41.0 32.0 41.0 18-19 37.42957205142219 39.0 36.0 41.0 32.0 41.0 20-21 37.41648421500984 39.0 36.0 41.0 32.0 41.0 22-23 37.36211647763665 39.0 36.0 41.0 32.0 41.0 24-25 37.299910958851655 39.0 36.0 41.0 31.0 41.0 26-27 36.970345453362086 39.0 36.0 41.0 30.5 41.0 28-29 36.80929353296532 39.0 36.0 40.5 30.0 41.0 30-31 36.6640466471541 39.0 36.0 40.0 30.0 41.0 32-33 36.541847443797096 39.0 35.5 40.0 30.0 41.0 34-35 36.48934786670076 39.0 35.0 40.0 30.0 41.0 36-37 36.37277807809073 38.5 35.0 40.0 30.0 41.0 38-39 36.27050863268565 38.0 35.0 40.0 30.0 41.0 40-41 36.16325437476726 38.0 35.0 40.0 29.5 41.0 42-43 35.96688926829022 38.0 35.0 40.0 29.0 41.0 44-45 35.82839661745916 38.0 35.0 40.0 28.0 41.0 46-47 35.69490307342264 38.0 34.5 40.0 28.0 41.0 48-49 35.52166270879722 38.0 34.0 40.0 27.0 41.0 50-51 35.57232554559438 38.0 34.0 40.0 27.5 41.0 52-53 35.45945261234975 38.0 34.0 40.0 27.0 41.0 54-55 35.39399568093178 38.0 34.0 40.0 27.0 41.0 56-57 35.19896803771276 38.0 34.0 40.0 26.5 41.0 58-59 35.03622946696974 38.0 34.0 40.0 26.0 41.0 60-61 34.79371536084666 38.0 33.5 40.0 26.0 41.0 62-63 34.55350819704684 37.5 33.0 40.0 26.0 41.0 64-65 34.29886047885942 37.0 33.0 40.0 26.0 41.0 66-67 34.05693963530025 37.0 33.0 40.0 25.5 41.0 68-69 33.79646506416548 36.0 32.0 39.0 25.0 41.0 70-71 33.47248310948564 36.0 32.0 39.0 25.0 40.5 72-73 33.088939853531265 35.5 32.0 39.0 24.0 40.0 74-75 32.70934406437206 35.0 31.5 38.0 23.5 40.0 76-77 31.671291951813252 34.0 30.5 36.5 22.0 39.0 78-79 31.97247088575474 35.0 31.0 37.0 22.0 39.0 80-81 31.83053246496947 35.0 31.0 36.0 23.0 39.0 82-83 31.571013590019707 35.0 31.0 36.0 22.5 37.5 84-85 31.276989467709157 34.0 31.0 36.0 22.0 37.0 86-87 30.95520068734288 34.0 31.0 35.0 20.5 37.0 88-89 30.609799837404424 34.0 31.0 35.0 20.0 36.0 90-91 30.427473049729308 34.0 31.0 35.0 19.5 36.0 92-93 30.24210412468345 34.0 31.0 35.0 18.5 36.0 94-95 29.99359853191495 34.0 31.0 35.0 16.0 35.5 96-97 29.67965510418972 34.0 30.0 35.0 4.5 35.0 98-99 29.20302979639181 34.0 30.0 35.0 2.0 35.0 100-101 27.679825313405857 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.072318617110298 1101 2 1.091642325378487 1101 3 1.3030388420838612 1101 4 2.5788675747458285 1101 5 1.9250062893033402 1101 6 1.2819106915164653 1101 7 0.7987978742326831 1101 8 0.731571126386342 1101 9 0.7612218208369583 1101 10-11 0.8231833877494239 1101 12-13 0.9166938242609319 1101 14-15 1.1370631006419032 1101 16-17 1.029554364335084 1101 18-19 0.8854064167775348 1101 20-21 0.8636910169667118 1101 22-23 0.7664490253412239 1101 24-25 0.7955462732752991 1101 26-27 0.8685421307513508 1101 28-29 0.9905589699826649 1101 30-31 1.035325364404386 1101 32-33 1.121267949689944 1101 34-35 0.9757190564189671 1101 36-37 0.8780421448458782 1101 38-39 0.8319559524935656 1101 40-41 0.7920030459292065 1101 42-43 0.8696494258823861 1101 44-45 0.9014605403614198 1101 46-47 0.8368838366925075 1101 48-49 0.7824105435008732 1101 50-51 0.7855050243775068 1101 52-53 0.9280769832552451 1101 54-55 0.9765690714168116 1101 56-57 0.9462064820447722 1101 58-59 0.9634679697630233 1101 60-61 0.9320814610020491 1101 62-63 0.9588931836236938 1101 64-65 0.9599345608895149 1101 66-67 0.839380162725682 1101 68-69 0.8998899311341191 1101 70-71 0.8433627111258275 1101 72-73 0.8047685529812298 1101 74-75 0.7430873465356598 1101 76-77 0.5295761652388862 1101 78-79 0.7351955638172569 1101 80-81 0.7433268877249617 1101 82-83 0.7564057965571322 1101 84-85 0.7663805804298569 1101 86-87 0.7663442750621314 1101 88-89 0.7348788936632822 1101 90-91 0.6694166596084692 1101 92-93 0.6201098275952148 1101 94-95 0.4999329346274912 1101 96-97 0.4581196048645637 1101 98-99 0.5288623920050775 1101 100-101 0.47405025618686025 1102 1 0.7165899062085614 1102 2 0.9095886936042206 1102 3 1.1773035227404947 1102 4 1.0852487422882078 1102 5 1.3280943287226066 1102 6 0.8945562119576138 1102 7 0.6922533344815847 1102 8 0.573438759479842 1102 9 0.600396720291215 1102 10-11 0.7426399438067719 1102 12-13 0.8318681677818986 1102 14-15 1.0534178472280402 1102 16-17 0.8668423816549691 1102 18-19 0.6588945373609079 1102 20-21 0.7201540313191401 1102 22-23 0.6993645579482575 1102 24-25 0.6581150379630074 1102 26-27 0.7454135215772908 1102 28-29 0.8219201872335091 1102 30-31 0.9385830180579333 1102 32-33 1.0395524400113132 1102 34-35 0.9905209109913571 1102 36-37 0.7530004696154222 1102 38-39 0.7533874636191058 1102 40-41 0.6168986904179974 1102 42-43 0.7021296354836721 1102 44-45 0.7726149224844789 1102 46-47 0.7940570768929902 1102 48-49 0.8224978337118429 1102 50-51 0.7591354495338081 1102 52-53 0.8757588289821854 1102 54-55 0.9284522173438958 1102 56-57 0.8945525011804492 1102 58-59 0.8764074888617159 1102 60-61 0.8619544073364196 1102 62-63 0.8908191936746022 1102 64-65 0.9580581927624436 1102 66-67 0.8568115368365241 1102 68-69 0.8088282679648842 1102 70-71 0.837449207964184 1102 72-73 0.7864485062828024 1102 74-75 0.7292142626180294 1102 76-77 0.559508319314201 1102 78-79 0.7467007931189471 1102 80-81 0.600481471496785 1102 82-83 0.6554089117439972 1102 84-85 0.6843790380852539 1102 86-87 0.5493342613070205 1102 88-89 0.5045715704345746 1102 90-91 0.41310961096705157 1102 92-93 0.3895587034488237 1102 94-95 0.4998969703120686 1102 96-97 0.5411917835833009 1102 98-99 0.6170496343861593 1102 100-101 0.626627377232591 1103 1 0.004113625841771551 1103 2 0.5297006524333732 1103 3 0.8018242008122307 1103 4 -2.3136812790346504 1103 5 0.09038164650216629 1103 6 0.3582656668676165 1103 7 0.3803269211410907 1103 8 0.4710639553873719 1103 9 0.5555884535959024 1103 10-11 0.6308604592839728 1103 12-13 0.7098933829415159 1103 14-15 0.8666068313593129 1103 16-17 0.7545705914092551 1103 18-19 0.6378630421839588 1103 20-21 0.764763889718985 1103 22-23 0.6962913616052546 1103 24-25 0.5531688748113908 1103 26-27 0.6813007345354691 1103 28-29 0.7208147612811473 1103 30-31 0.792036094201336 1103 32-33 0.8407923767516721 1103 34-35 0.8179186325390972 1103 36-37 0.6908610102251416 1103 38-39 0.692238183110689 1103 40-41 0.6470290380662647 1103 42-43 0.7400813627771186 1103 44-45 0.7994413737838713 1103 46-47 0.8012902546777099 1103 48-49 0.7200032417116304 1103 50-51 0.5467091011152334 1103 52-53 0.7473391824839837 1103 54-55 0.9416399341253268 1103 56-57 0.7768741863623134 1103 58-59 0.8249786587614025 1103 60-61 0.8007807837177268 1103 62-63 0.7565818779644999 1103 64-65 0.7570766165967626 1103 66-67 0.7300679527732612 1103 68-69 0.7746114302660416 1103 70-71 0.8114249607654855 1103 72-73 0.7331186825000842 1103 74-75 0.7093505962457485 1103 76-77 0.6069018395836174 1103 78-79 0.7000426946093228 1103 80-81 0.5386899894864001 1103 82-83 0.665946253156676 1103 84-85 0.7042006793186992 1103 86-87 0.6689457117980666 1103 88-89 0.42945145142600794 1103 90-91 0.5329776328436964 1103 92-93 0.45983343732938664 1103 94-95 0.48719436161497853 1103 96-97 0.45938725726344387 1103 98-99 0.5761733826049671 1103 100-101 0.5002641832710921 1104 1 0.5390512421941942 1104 2 0.75353708632883 1104 3 0.8019541205698921 1104 4 0.3594639150006316 1104 5 1.0843730775775242 1104 6 0.7485077295174847 1104 7 0.5296726157454827 1104 8 0.418869548859945 1104 9 0.5187996941210002 1104 10-11 0.5745075429206068 1104 12-13 0.6223463120362283 1104 14-15 0.8229434920481751 1104 16-17 0.6977236552398765 1104 18-19 0.5669138090484651 1104 20-21 0.6594668883013952 1104 22-23 0.5847838855412988 1104 24-25 0.4952516735372754 1104 26-27 0.5887888919898785 1104 28-29 0.6453748175259122 1104 30-31 0.7145267670007058 1104 32-33 0.8416501744563476 1104 34-35 0.7202305318990128 1104 36-37 0.5759506441947764 1104 38-39 0.5066652520882187 1104 40-41 0.5932271882310758 1104 42-43 0.6922072126241687 1104 44-45 0.6602531900935915 1104 46-47 0.6494388699429408 1104 48-49 0.6021312100151874 1104 50-51 0.5190575045477104 1104 52-53 0.6249329741178045 1104 54-55 0.6452022817565748 1104 56-57 0.6977685815629542 1104 58-59 0.731675929006478 1104 60-61 0.6449606288848457 1104 62-63 0.6523636657330485 1104 64-65 0.5855507966838616 1104 66-67 0.6040456042168003 1104 68-69 0.6003049992686371 1104 70-71 0.5857815520839154 1104 72-73 0.5585606976779758 1104 74-75 0.5851872673722553 1104 76-77 0.2753425090132957 1104 78-79 0.4870421464393644 1104 80-81 0.45150563660720877 1104 82-83 0.49014250895231015 1104 84-85 0.5633616619758328 1104 86-87 0.5761539388703163 1104 88-89 0.6038974876121834 1104 90-91 0.5519682827060031 1104 92-93 0.4592214752920256 1104 94-95 0.43318443850180444 1104 96-97 0.45945223002826197 1104 98-99 0.5369904322367702 1104 100-101 0.4721028717635285 1105 1 -0.06276659263687634 1105 2 0.4396463512203965 1105 3 -0.04140682104294058 1105 4 -1.3726387816547678 1105 5 -0.4789396576375964 1105 6 0.0718867268655714 1105 7 0.26288224507202784 1105 8 0.28644214378135757 1105 9 0.3642551572123338 1105 10-11 0.4228810426574299 1105 12-13 0.6099990657237697 1105 14-15 0.7644907245468389 1105 16-17 0.7213154357755869 1105 18-19 0.6126261383798806 1105 20-21 0.6080485702716203 1105 22-23 0.5771068257896772 1105 24-25 0.44294713090314275 1105 26-27 0.5765623687328798 1105 28-29 0.6171493862384452 1105 30-31 0.7017675747429166 1105 32-33 0.7692045104124077 1105 34-35 0.6916298336850488 1105 36-37 0.5698060324084011 1105 38-39 0.5834745125795067 1105 40-41 0.5786228692011193 1105 42-43 0.690470691723462 1105 44-45 0.7172553708583322 1105 46-47 0.7007755420147248 1105 48-49 0.6336659916603224 1105 50-51 0.5393024356716722 1105 52-53 0.6345124965219711 1105 54-55 0.7057124830682042 1105 56-57 0.6419380286873277 1105 58-59 0.6772026831722755 1105 60-61 0.6416594998595642 1105 62-63 0.6831701940383326 1105 64-65 0.6238673766694234 1105 66-67 0.5958188772918689 1105 68-69 0.591715092499804 1105 70-71 0.6308121575512544 1105 72-73 0.6307008598198394 1105 74-75 0.5163164261915796 1105 76-77 0.3558411902433498 1105 78-79 0.5073340129520005 1105 80-81 0.4293354934653557 1105 82-83 0.4944541521978927 1105 84-85 0.5521701244697361 1105 86-87 0.5475409373909343 1105 88-89 0.42695672867438716 1105 90-91 0.42011720734879177 1105 92-93 0.4105919320868736 1105 94-95 0.45742938943797284 1105 96-97 0.437362825241415 1105 98-99 0.49705973734819153 1105 100-101 0.4726400933738688 1106 1 -0.592647744567703 1106 2 0.134612181539147 1106 3 -1.775662232680336 1106 4 -2.9419798217099924 1106 5 -0.47282450099812934 1106 6 -0.061783935915322274 1106 7 0.13605250702209304 1106 8 0.20627677139223977 1106 9 0.2733923516780905 1106 10-11 0.3950816630437899 1106 12-13 0.5301470357227203 1106 14-15 0.6179264426302566 1106 16-17 0.46141679690123283 1106 18-19 0.45606864975700745 1106 20-21 0.5073499911155253 1106 22-23 0.47791298871210586 1106 24-25 0.3432563075803685 1106 26-27 0.40245754083355223 1106 28-29 0.5078152987672055 1106 30-31 0.6235303117339441 1106 32-33 0.6789476482286076 1106 34-35 0.4916038370549032 1106 36-37 0.49147719114026245 1106 38-39 0.46047430214866836 1106 40-41 0.37297393469827966 1106 42-43 0.42237432018036714 1106 44-45 0.5506503960167777 1106 46-47 0.5215590829297057 1106 48-49 0.39913610585649906 1106 50-51 0.4313592315173693 1106 52-53 0.6049524342526524 1106 54-55 0.5881352271531526 1106 56-57 0.5746158380951485 1106 58-59 0.5123705326345984 1106 60-61 0.4451776869164945 1106 62-63 0.4771101802579665 1106 64-65 0.6046344043309944 1106 66-67 0.5408964695762037 1106 68-69 0.4651816812209191 1106 70-71 0.48418839242047085 1106 72-73 0.4717174567572613 1106 74-75 0.4803587380107288 1106 76-77 0.27162336923323593 1106 78-79 0.3654340500689415 1106 80-81 0.32031806488049597 1106 82-83 0.49841441963044986 1106 84-85 0.4821143247197526 1106 86-87 0.416210852483367 1106 88-89 0.37332833034571067 1106 90-91 0.3316933725581883 1106 92-93 0.3127431957797029 1106 94-95 0.29667026127302165 1106 96-97 0.3071793544215211 1106 98-99 0.446903586892212 1106 100-101 0.4272783368775279 1107 1 -0.22420982757602204 1107 2 0.24904575284213237 1107 3 0.057044556000132474 1107 4 -1.2913897575367344 1107 5 -0.9883556383704288 1107 6 -0.14341570357905908 1107 7 -0.8964106493612007 1107 8 -0.15775399470117435 1107 9 -1.046272143045016 1107 10-11 0.14369334201587236 1107 12-13 0.4001385465134035 1107 14-15 0.5272185068724013 1107 16-17 0.44072657769520873 1107 18-19 -0.2314521424172611 1107 20-21 0.174505258434138 1107 22-23 0.3259276932742665 1107 24-25 -0.5466779429401782 1107 26-27 0.3193096957144661 1107 28-29 0.5386255136059432 1107 30-31 0.5274743928534136 1107 32-33 -0.2987730027085007 1107 34-35 0.025598415887351678 1107 36-37 0.35836388406460173 1107 38-39 0.32198530282479965 1107 40-41 0.36096467226142437 1107 42-43 0.5471981813529041 1107 44-45 0.5482823492079874 1107 46-47 0.5431633900686634 1107 48-49 0.42203664298703103 1107 50-51 0.5020071093003295 1107 52-53 0.5116918272771684 1107 54-55 0.5314344223678162 1107 56-57 0.5401611371243007 1107 58-59 0.4747576609523918 1107 60-61 0.3796373225487599 1107 62-63 0.4857268437488287 1107 64-65 0.5902330783612229 1107 66-67 0.64812899247719 1107 68-69 0.6557382031504275 1107 70-71 0.6297646459216182 1107 72-73 0.5657576796212282 1107 74-75 0.41592337827312775 1107 76-77 0.3605249395403156 1107 78-79 0.42526588232458096 1107 80-81 0.3475955969893434 1107 82-83 0.49342719003374924 1107 84-85 0.4913606889245692 1107 86-87 0.5809778722188099 1107 88-89 0.5923897314208766 1107 90-91 0.5796254771588423 1107 92-93 0.4599043896504931 1107 94-95 0.5162264949605166 1107 96-97 0.528490444023042 1107 98-99 0.6252931356816056 1107 100-101 0.778067317689306 1108 1 -1.270917189640592 1108 2 -0.004207735739047536 1108 3 -2.5545134798215727 1108 4 -3.4609009882585866 1108 5 -2.9209652822024523 1108 6 -1.0785504346762451 1108 7 -0.22352978524179434 1108 8 -0.1432547339710908 1108 9 -1.128707858483132 1108 10-11 -0.7782925462912615 1108 12-13 0.27087325457847555 1108 14-15 0.5883639688759743 1108 16-17 0.5544807202056674 1108 18-19 0.38976714401787405 1108 20-21 0.5157992572607029 1108 22-23 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0.5349164112636942 2310 70-71 0.49311305646474324 2310 72-73 0.5006172016830206 2310 74-75 0.5177326374469544 2310 76-77 0.41242251210464076 2310 78-79 0.5313726806657968 2310 80-81 0.36317429681940183 2310 82-83 0.41629387792755423 2310 84-85 0.483902298925873 2310 86-87 0.37666517255447474 2310 88-89 0.40415620425378407 2310 90-91 0.39434924183283826 2310 92-93 0.3338759791880257 2310 94-95 0.3373494677334854 2310 96-97 0.3601649573776342 2310 98-99 0.46884406237082743 2310 100-101 0.5599432010885579 2311 1 -0.1729714753371745 2311 2 0.030102797925167124 2311 3 -0.06662558851832401 2311 4 1.9952382120312109 2311 5 -2.67712544099248 2311 6 -0.674115179541289 2311 7 -0.19219528530990004 2311 8 -0.10089903505697606 2311 9 -0.034650275135128084 2311 10-11 0.22375869050642194 2311 12-13 0.3456872032860474 2311 14-15 0.40792779529567014 2311 16-17 0.2426852032501685 2311 18-19 0.22731967788035234 2311 20-21 0.20114865291348138 2311 22-23 0.16840344099645677 2311 24-25 -1.014492858436924 2311 26-27 -0.3652336137869483 2311 28-29 -0.4029896427027424 2311 30-31 0.1745333998752301 2311 32-33 0.31166117160955764 2311 34-35 0.26631659220380044 2311 36-37 0.3000496496355396 2311 38-39 0.2596135564391773 2311 40-41 0.2231752953078896 2311 42-43 0.3384295845907488 2311 44-45 0.2824237243896519 2311 46-47 0.4061712886942743 2311 48-49 0.32315742794167335 2311 50-51 0.17534197925447614 2311 52-53 0.2681620346658633 2311 54-55 0.2077720346903007 2311 56-57 0.11245279784855455 2311 58-59 0.2327013357466896 2311 60-61 0.16794115127176212 2311 62-63 0.09762593046063728 2311 64-65 0.24844193355531274 2311 66-67 0.3083933596300241 2311 68-69 0.23584768799593547 2311 70-71 0.3211439044775446 2311 72-73 0.34554444022170117 2311 74-75 0.2795749614294749 2311 76-77 0.18374341044368592 2311 78-79 0.31350194953319743 2311 80-81 0.3028915101659564 2311 82-83 0.2708954096317626 2311 84-85 0.25954037695910515 2311 86-87 0.27891711132016894 2311 88-89 0.32105045860391357 2311 90-91 0.1558581835192463 2311 92-93 0.13402748479389004 2311 94-95 0.10977975115690342 2311 96-97 0.0680236126031275 2311 98-99 0.3029340940014542 2311 100-101 0.46570047976061346 2312 1 -2.3239593840857005 2312 2 -3.476744253971635 2312 3 -4.54797566227753 2312 4 -4.587838928375994 2312 5 -3.7518772886133682 2312 6 -2.5545042684499855 2312 7 -0.9682177973809445 2312 8 -0.5403948598149242 2312 9 -0.2503032797828624 2312 10-11 -0.27044283653754775 2312 12-13 -0.10179839855819495 2312 14-15 0.10076273596514085 2312 16-17 0.21494983476892315 2312 18-19 -0.04903890567033642 2312 20-21 0.017100300102512733 2312 22-23 0.07695231063414099 2312 24-25 0.03868564803792651 2312 26-27 0.05105502675097284 2312 28-29 0.19709134767850145 2312 30-31 0.23305843381645275 2312 32-33 0.30097415373226966 2312 34-35 0.19369982636886363 2312 36-37 0.045046595988985416 2312 38-39 0.12910837201410175 2312 40-41 0.2826481187637384 2312 42-43 -1.4225130617070363 2312 44-45 -0.0669073420770161 2312 46-47 0.35546446710030466 2312 48-49 0.38242983444681045 2312 50-51 0.2671989092883038 2312 52-53 0.20909026259040786 2312 54-55 -0.07757068012787016 2312 56-57 0.06587802456314051 2312 58-59 0.19146896588176077 2312 60-61 -0.6932582627947497 2312 62-63 0.011465934862407323 2312 64-65 0.14347423876937881 2312 66-67 0.27879059377099935 2312 68-69 0.24784112024344296 2312 70-71 0.30291382543956047 2312 72-73 0.4225432363869359 2312 74-75 0.4964307396301706 2312 76-77 0.2379358965641778 2312 78-79 0.37330377640175527 2312 80-81 0.33313594172132355 2312 82-83 0.14205142834487816 2312 84-85 0.2049953694903941 2312 86-87 0.3752841990239979 2312 88-89 0.3426155919320628 2312 90-91 0.378481723824482 2312 92-93 0.27381605760723815 2312 94-95 0.21053835771847673 2312 96-97 0.26144572236061236 2312 98-99 0.456551635769177 2312 100-101 0.5576822527987773 2313 1 -0.2804887410388943 2313 2 -1.5640333270494153 2313 3 -0.0865564381689019 2313 4 1.711117395331165 2313 5 1.2482784031420877 2313 6 0.11524415924785814 2313 7 -0.04249401697879307 2313 8 -0.5113802083256189 2313 9 -1.3509496493177267 2313 10-11 -1.7915430639338936 2313 12-13 -0.45438401410112306 2313 14-15 -0.4419986829418079 2313 16-17 -0.4045679223715908 2313 18-19 -1.3170025865765567 2313 20-21 -0.7051522494338229 2313 22-23 -0.41711253742847987 2313 24-25 -0.9436805425103358 2313 26-27 -1.4512940582604301 2313 28-29 -0.9475337211444597 2313 30-31 -0.7140991688411731 2313 32-33 -1.5796572406467106 2313 34-35 -0.9992134309648222 2313 36-37 -0.7978532292815856 2313 38-39 -0.3591056559390111 2313 40-41 -0.2183844860550721 2313 42-43 -0.04907553330500036 2313 44-45 -1.2874126410083946 2313 46-47 -0.6417787543815265 2313 48-49 -0.1268643671144929 2313 50-51 -0.5771194179446155 2313 52-53 -0.7934114292334584 2313 54-55 -1.8127518671260603 2313 56-57 -1.289228298526119 2313 58-59 -0.5284287925994491 2313 60-61 -0.9774097430532365 2313 62-63 -0.6100567089001814 2313 64-65 -0.925502042748569 2313 66-67 -0.6050858831672485 2313 68-69 -0.8565827630320513 2313 70-71 -0.16741114682749725 2313 72-73 0.03012301754237967 2313 74-75 -0.32036272863817317 2313 76-77 -0.37873717973075927 2313 78-79 0.040920028670598185 2313 80-81 0.01982886104268644 2313 82-83 -0.08929504393545074 2313 84-85 -0.5182304266290103 2313 86-87 -0.8013279041162562 2313 88-89 -0.9118877727928414 2313 90-91 -0.5142248120497861 2313 92-93 -0.4237619613457717 2313 94-95 -0.26986654707704005 2313 96-97 -0.2291593677237742 2313 98-99 -0.028923690846575312 2313 100-101 0.0019613684128572118 2314 1 0.40342599383669153 2314 2 0.209734420293195 2314 3 0.601217622018023 2314 4 1.8687662495667325 2314 5 1.4301865277848336 2314 6 0.5776844800771315 2314 7 -0.990005155542967 2314 8 -1.0170717494832857 2314 9 -2.027392226360888 2314 10-11 -2.231593169515129 2314 12-13 -1.3707451032790345 2314 14-15 -3.0226155815166535 2314 16-17 -2.226748468801439 2314 18-19 -0.6263564662252747 2314 20-21 -1.0345569857618386 2314 22-23 -0.8455709720271045 2314 24-25 -1.9238127652019372 2314 26-27 -1.4990403841062232 2314 28-29 -1.6070683260762095 2314 30-31 -1.8643048146725718 2314 32-33 -1.1124859097448407 2314 34-35 -2.652314124387466 2314 36-37 -2.587899907857917 2314 38-39 -1.402461870600689 2314 40-41 -1.322441994390978 2314 42-43 -1.9037211275508952 2314 44-45 -1.220013673155087 2314 46-47 -1.2794547487349277 2314 48-49 -1.4457724121169235 2314 50-51 -1.2279626608631062 2314 52-53 -2.244633655947254 2314 54-55 -3.109492492266469 2314 56-57 -3.8731670751621152 2314 58-59 -1.6182917441259264 2314 60-61 -2.1488418467828723 2314 62-63 -5.058979046423509 2314 64-65 -2.526272245833969 2314 66-67 -1.4095735065771606 2314 68-69 -1.692293736235797 2314 70-71 -1.0045745064078702 2314 72-73 -1.7155607464914624 2314 74-75 -2.6372563932558677 2314 76-77 -2.0594619638712466 2314 78-79 -2.9654950358549286 2314 80-81 -2.657693276957236 2314 82-83 -1.770415003595616 2314 84-85 -1.390412205089401 2314 86-87 -1.3962948881952286 2314 88-89 -1.2847956385841393 2314 90-91 -1.678696418395372 2314 92-93 -1.6455189610367427 2314 94-95 -1.8500036805545257 2314 96-97 -1.824374045349618 2314 98-99 -1.4544426991241046 2314 100-101 -2.320014523690645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9157.0 3 1208.0 4 1568.0 5 2883.0 6 6979.0 7 16980.0 8 35645.0 9 52812.0 10 66452.0 11 77719.0 12 85742.0 13 95719.0 14 108723.0 15 123179.0 16 139705.0 17 156046.0 18 173007.0 19 191723.0 20 207781.0 21 199532.0 22 225684.0 23 266543.0 24 328337.0 25 420586.0 26 543780.0 27 699155.0 28 884699.0 29 1103534.0 30 1355351.0 31 1641396.0 32 1973995.0 33 2379991.0 34 2916720.0 35 3647953.0 36 4800291.0 37 6687173.0 38 6872461.0 39 1583076.0 40 2676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.804917902645013 16.64571140125445 18.20124536171565 33.34812533438489 2 20.72280786047062 20.883525239987783 41.77369870374604 16.619968195795554 3 23.933723833139393 23.054136333131282 32.83600080846741 20.176139025261914 4 23.867178704142635 30.530149684045476 28.599680984446728 17.002990627365154 5 23.669505273283296 33.25245254878249 28.010816413125596 15.067225764808622 6 18.277321578993703 36.370294328231275 28.92582517854568 16.426558914229346 7 16.40954298189334 18.825640727435722 47.08523265788738 17.679583632783558 8 17.99626058609397 24.369312238766085 36.85993707373012 20.774490101409818 9 19.68490414886149 22.044535741577956 37.09766119864259 21.172898910917965 10-11 21.179152970786955 33.20962917665863 27.927186278507833 17.684031574046585 12-13 19.684771932996693 27.41926157140152 34.146917420789784 18.749049074812003 14-15 20.04698070494066 27.58152657582336 33.86094699049597 18.510545728740013 16-17 20.779298502573145 27.847072327584065 31.91097797700938 19.46265119283341 18-19 21.03469466529691 28.249346448249053 30.351720893007904 20.364237993446135 20-21 20.969543426936927 28.530341333216384 30.078848552489486 20.421266687357203 22-23 20.793082695028982 28.58648122384301 31.47301691688375 19.14741916424426 24-25 20.621634454542896 28.53486172978442 31.470847264736197 19.372656550936483 26-27 20.95041422114648 28.72814852162302 30.436504407506217 19.884932849724287 28-29 21.416413956074535 28.495246331798384 29.70124446134229 20.38709525078479 30-31 20.951706232095553 28.136404774025326 30.53763623083279 20.374252763046332 32-33 21.48998933227838 28.453399453860364 30.168103889150714 19.888507324710538 34-35 20.346341426290238 29.850003262534464 29.412927197073675 20.390728114101623 36-37 20.619800016370647 28.84790258295044 29.81699570788893 20.715301692789982 38-39 21.020116577144833 28.080631391935025 30.436367883217798 20.462884147702344 40-41 21.344251137695462 28.44356239421445 29.79117499063728 20.421011477452808 42-43 21.06141873046184 29.073822443341196 29.916327302043477 19.948431524153488 44-45 21.01383398099863 28.824596350324438 29.968635074241845 20.19293459443509 46-47 20.89013214693364 28.701971426613653 29.768340491893202 20.639555934559503 48-49 21.410026844639816 28.638713130391668 29.189348379745066 20.761911645223442 50-51 21.188013963028844 28.701558465526894 29.4479453396232 20.662482231821063 52-53 20.975641813251308 28.760549994499172 29.877185089487064 20.386623102762456 54-55 20.685448561426377 28.81355419058399 29.950456307026652 20.550540940962982 56-57 21.134560288748343 28.35604364001058 29.452317897246594 21.05707817399448 58-59 21.261607385054262 28.330202430153477 29.29339416469429 21.114796020097966 60-61 21.458341408761285 28.289437310189886 29.551269877947604 20.700951403101225 62-63 20.457888733857352 29.5360174927848 29.446377185393242 20.55971658796461 64-65 20.25056978464764 29.666650441475433 29.361161138784077 20.721618635092845 66-67 20.600580433376354 28.78601535486927 29.82041016042119 20.79299405133318 68-69 21.184315316941447 29.132717543604585 29.054846159654357 20.62812097979961 70-71 20.709191099554054 29.899199009782766 28.75332437118346 20.63828551947972 72-73 20.433954869821005 29.743190225889993 29.120058912350682 20.702795991938316 74-75 20.528313338922295 28.937471905048657 29.66045459358731 20.873760162441737 76-77 20.767971989714972 29.005014028588622 29.090941830203278 21.136072151493128 78-79 20.773937978848267 29.526722974984015 28.593349100342213 21.105989945825502 80-81 20.873256360352084 29.500871922960542 29.31637119430368 20.30950052238369 82-83 20.837460902282395 29.460929631126735 29.216595965154667 20.485013501436203 84-85 20.659449503846236 29.71570924425977 28.812414455090035 20.81242679680396 86-87 21.057210724667716 30.023650553989178 28.07870113474164 20.840437586601464 88-89 20.832814203603974 29.710132804768513 28.615778018243947 20.84127497338357 90-91 20.915859043250645 30.02866083962914 28.324403486911855 20.73107663020836 92-93 20.452963814879304 30.452718515543243 28.228613391477182 20.865704278100274 94-95 20.48868562799253 30.512342987006086 28.168046181296603 20.83092520370478 96-97 20.810427877737126 29.56999526235153 28.716497308544497 20.90307955136685 98-99 21.091723144843456 29.936085577772108 27.92687665361867 21.045314623765766 100-101 21.355551399355715 30.488683248506426 27.339316164700715 20.81644918743714 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 799.0 1 873.0 2 1131.5 3 1690.5 4 2588.5 5 3625.0 6 4245.5 7 4638.0 8 5665.0 9 6842.5 10 8782.0 11 12898.5 12 16431.5 13 21814.5 14 26312.5 15 38138.0 16 81219.0 17 115510.5 18 140291.0 19 163874.5 20 179809.0 21 167934.5 22 139719.5 23 157144.5 24 212495.5 25 270295.0 26 318149.5 27 362309.5 28 424568.0 29 511587.5 30 618929.0 31 757606.5 32 931032.5 33 1148986.0 34 1391721.0 35 1645091.0 36 1869830.5 37 1987738.0 38 1909926.0 39 1722973.5 40 1590983.5 41 1519573.0 42 1469059.5 43 1428704.5 44 1390770.0 45 1361720.5 46 1341368.0 47 1322958.0 48 1301119.5 49 1249262.0 50 1167801.5 51 1095179.5 52 1017351.5 53 907821.5 54 801705.5 55 709919.5 56 623370.5 57 531272.0 58 441578.0 59 356642.5 60 274475.5 61 208862.0 62 157722.0 63 116001.5 64 84393.0 65 60895.5 66 43374.5 67 31974.5 68 23842.5 69 17057.5 70 12447.5 71 8965.5 72 6801.0 73 5832.0 74 4874.5 75 3467.5 76 2477.5 77 1968.0 78 1631.5 79 1258.5 80 963.5 81 815.5 82 648.0 83 546.0 84 466.5 85 365.0 86 276.0 87 190.0 88 125.5 89 84.0 90 64.5 91 50.5 92 31.5 93 15.5 94 8.0 95 6.0 96 3.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1661030404135752 2 1.646461712618041E-4 3 5.962187110844119E-4 4 0.003480021347124496 5 5.338527371216073E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.03380859448523636 16-17 9.903716665293368E-4 18-19 0.0 20-21 0.0 22-23 6.2365973962804585E-6 24-25 0.013341329150123158 26-27 0.06671537698697057 28-29 0.05738916924057278 30-31 0.048070445411050516 32-33 0.027096768367359338 34-35 0.02433769767924486 36-37 0.02028266205218331 38-39 0.023044227379256293 40-41 0.02026270494051521 42-43 0.02669887345347664 44-45 0.03127279398390873 46-47 0.0282567754830675 48-49 0.04164924473184016 50-51 0.15068741896944918 52-53 0.2974457815792417 54-55 0.2986669073494334 56-57 0.3845860150390307 58-59 0.3793435312677174 60-61 0.37418835985995197 62-63 0.3581054224944239 64-65 0.3693687173921064 66-67 0.3323545118451819 68-69 0.2562592923742055 70-71 0.25044054700347584 72-73 0.255686772733227 74-75 0.26587986751770776 76-77 0.2510816692158135 78-79 0.21766223840810503 80-81 0.22565256699221956 82-83 0.20981285692514645 84-85 0.14744688296234187 86-87 0.04959466981470146 88-89 0.017126943769665396 90-91 0.007163355769367734 92-93 0.031115631729522465 94-95 0.018239552745161827 96-97 0.038539677270054716 98-99 0.05000004864545969 100-101 0.043174716454970355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.0085961E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.779812094116224 #Duplication Level Percentage of deduplicated Percentage of total 1 85.73962154317215 52.112380864146424 2 7.912943443698758 9.61894431238759 3 2.489875057108417 4.540024143266295 4 1.1657684831841222 2.8342075741269537 5 0.6290990265363121 1.9118260310734243 6 0.4098888434111678 1.4947780129203248 7 0.2786085644831527 1.1853643337968238 8 0.215394906722118 1.047332956528002 9 0.1547414065456657 0.8464638251722328 >10 0.8916250508761208 9.970409929336855 >50 0.06804041959783598 2.8637751313401476 >100 0.039042551946019625 4.444662523804073 >500 0.002697075600104757 1.1107987412268987 >1k 0.002223611918517521 2.7672207828618003 >5k 2.6048999503483576E-4 1.0968351134501566 >10k+ 1.695252043610362E-4 2.154975724562243 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 63626 0.15872389837429618 No Hit GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT 50251 0.12535810230419572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006473588097339116 0.0 0.0 4.989277917024367E-6 0.0 2 0.006503523764841262 4.989277917024367E-6 0.0 1.9957111668097467E-5 0.0 3 0.00655840582192853 7.4839168755365504E-6 0.0 1.9957111668097467E-5 0.0 4 0.0067280412711073585 7.4839168755365504E-6 0.0 3.741958437768275E-5 0.0 5 0.006797891161945699 7.4839168755365504E-6 0.0 4.49035012532193E-5 0.0 6 0.006882708886535114 1.2473194792560917E-5 0.0 5.737669604578022E-5 0.0 7 0.006947569499456431 1.4967833751073101E-5 0.0 5.9871335004292403E-5 0.0 8 0.007034881863004357 1.4967833751073101E-5 0.0 6.236597396280459E-5 0.0 9 0.007214495868017234 1.7462472709585285E-5 0.0 7.48391687553655E-5 0.0 10-11 0.007607401503982904 2.245175062660965E-5 0.0 7.858112719313378E-5 0.0 12-13 0.008193641659233267 2.7441028543634016E-5 0.0 9.230164146495077E-5 0.0 14-15 0.008887151289699653 2.7441028543634016E-5 0.0 1.060221557367678E-4 0.0 16-17 0.009822640899141722 2.7441028543634016E-5 0.0 1.22237308967097E-4 0.0 18-19 0.010919034721407827 2.868834802289011E-5 0.0 1.309685453218896E-4 0.0 20-21 0.012118956060452187 2.9935667502146202E-5 0.0 1.4468905959370663E-4 0.0 22-23 0.013356296983874229 3.367762593991448E-5 0.0 1.6963544917882848E-4 0.0 24-25 0.014637294089070236 3.492494541917057E-5 0.0 1.90839880326182E-4 1.2473194792560917E-6 26-27 0.016031797266878547 4.240886229470712E-5 0.0 2.120443114735356E-4 2.4946389585121833E-6 28-29 0.01751984940563106 4.49035012532193E-5 0.0 2.2451750626609651E-4 4.989277917024367E-6 30-31 0.019041579170323494 4.989277917024367E-5 0.0 2.394853400171696E-4 4.989277917024367E-6 32-33 0.020720471189402193 5.2387418128755854E-5 0.0 2.519585348097305E-4 4.989277917024367E-6 34-35 0.022280867857951567 5.612937656652413E-5 0.0 2.631844101230353E-4 4.989277917024367E-6 36-37 0.02395102864067547 6.486061292131677E-5 0.0 2.7939956335336455E-4 4.989277917024367E-6 38-39 0.02556131808839509 6.735525187982895E-5 0.0 2.931200776251816E-4 6.2365973962804585E-6 40-41 0.027240210107473788 7.234452979685332E-5 0.0 3.018513139799742E-4 7.4839168755365504E-6 42-43 0.029028866240727022 7.234452979685332E-5 0.0 3.1931378668955947E-4 8.731236354792642E-6 44-45 0.03084870536096166 7.48391687553655E-5 0.0 3.2679770356509603E-4 9.978555834048733E-6 46-47 0.032841921888812894 7.48391687553655E-5 0.0 3.4675481523319346E-4 9.978555834048733E-6 48-49 0.035044688089179155 7.982844667238987E-5 0.0 3.6795924638054705E-4 9.978555834048733E-6 50-51 0.037559284159359434 8.107576615164596E-5 0.0 3.779378022145958E-4 9.978555834048733E-6 52-53 0.04020484877486161 9.354896094420688E-5 0.0 3.9041099700715674E-4 9.978555834048733E-6 54-55 0.04304374790964847 9.978555834048734E-5 0.0 4.0038955284120545E-4 9.978555834048733E-6 56-57 0.04636161772446967 9.978555834048734E-5 0.0 4.0662615023748586E-4 9.978555834048733E-6 58-59 0.050359276655485445 1.0228019729899952E-4 0.0 4.178520255507907E-4 9.978555834048733E-6 60-61 0.055059176453322395 1.0352751677825562E-4 0.0 4.2907790086409555E-4 9.978555834048733E-6 62-63 0.0612433864314741 1.0726947521602389E-4 1.2473194792560917E-6 4.452930540944247E-4 9.978555834048733E-6 64-65 0.06930605954538549 1.0851679469527998E-4 2.4946389585121833E-6 4.5402429044921733E-4 1.2473194792560917E-5 66-67 0.07990453316062449 1.1350607261230435E-4 2.4946389585121833E-6 4.6150820732475395E-4 1.2473194792560917E-5 68-69 0.09415515821112533 1.1724803105007261E-4 2.4946389585121833E-6 4.75228721596571E-4 1.2473194792560917E-5 70-71 0.11391145144306258 1.1974267000858481E-4 2.4946389585121833E-6 4.964331527439245E-4 1.2473194792560917E-5 72-73 0.14077996034571805 1.1974267000858481E-4 2.4946389585121833E-6 5.126483059742536E-4 1.2473194792560917E-5 74-75 0.17732517376844226 1.1974267000858481E-4 2.4946389585121833E-6 5.276161397253269E-4 1.2473194792560917E-5 76-77 0.2263722703317503 1.1974267000858481E-4 2.4946389585121833E-6 5.400893345178877E-4 1.2473194792560917E-5 78-79 0.29213344791708995 1.1974267000858481E-4 2.4946389585121833E-6 5.600464461859851E-4 1.2473194792560917E-5 80-81 0.38002706234234973 1.2223730896709697E-4 2.4946389585121833E-6 5.700250020200339E-4 1.2473194792560917E-5 82-83 0.49526067243342375 1.2223730896709697E-4 2.4946389585121833E-6 5.787562383748265E-4 1.2473194792560917E-5 84-85 0.6434522051248814 1.2348462844635307E-4 2.4946389585121833E-6 6.037026279599484E-4 1.2473194792560917E-5 86-87 0.8337657166308174 1.2597926740486525E-4 2.4946389585121833E-6 6.348856149413506E-4 1.2473194792560917E-5 88-89 1.0651833942561586 1.2722658688412136E-4 2.4946389585121833E-6 6.411222123376311E-4 1.372051427181701E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCAG 13955 0.0 30.937077 1 CGACGGC 4365 0.0 24.373001 1 ACGACGG 3790 0.0 22.306223 1 GACGGCC 6810 0.0 20.609636 2 CACGGTC 19290 0.0 20.435774 1 TCACGGT 17040 0.0 19.673225 2 CGGCGAG 2450 0.0 19.57945 1 TTACGCG 1215 0.0 17.95074 7 TACGCGC 1375 0.0 16.551577 8 ACGGCCA 8985 0.0 15.778917 3 CTGGAAT 86800 0.0 15.392006 1 CGGCGCT 5060 0.0 14.830364 1 ACGGTCT 26890 0.0 14.670952 2 CGGACGT 1215 0.0 14.463408 1 GGCCAGT 31055 0.0 13.955339 2 TAATACG 48610 0.0 13.635338 9 TATGGCG 108810 0.0 13.630092 7 TGGCGAG 109610 0.0 13.323353 9 ATGGCGA 111955 0.0 13.264139 8 TGAATTG 47570 0.0 13.237035 2 >>END_MODULE