FastQCFastQC Report
Fri 27 May 2016
SRR1233110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70259079
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC8055521.1465450607458147No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG5818120.8280951135155072No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG5206800.7410857179041587No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA4664570.6639099268579937No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG3978170.566214367825687No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA3407780.4850305538448632No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT3338620.4751869861544869No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA3319110.47241012083292466No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA2995470.42634632315632826No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC2910570.414262475601196No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG2890460.4114002120636964No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT2774960.39496105549576016No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC2579470.3671368934397788No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC2544880.36221368629099165No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT2534050.3606722484933228No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA2150170.3060344699366184No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG2125360.3025032537076098No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT2083800.2965880039503507No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA2063580.2937100840732626No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA1980470.2818810078623433No Hit
ATACGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAAT1907470.27149089158996803No Hit
GGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAAGT1899250.2703209360316266No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT1817740.2587195883965402No Hit
ATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGT1785180.25408531187834105No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA1687080.2401227035725874No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA1615110.22987918757090453No Hit
ATACGACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTA1530190.21779249340857426No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC1471900.2094960567302626No Hit
ATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAA1410070.2006957705779206No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT1376200.19587504128825828No Hit
TAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTT1361790.19382406080216338No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA1342590.19109131789216877No Hit
GGCAATTGTAATACGACTCACTATAGGCAATTGTAATACGACTCACTATA1339920.19071129583124766No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA1313020.18688260915005733No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG1285690.18299272041411188No Hit
ATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTAT1262640.1797120056185194No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT1250100.1779271829054292No Hit
GTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATA1210490.1722894773499664No Hit
CGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTAC1198120.17052885079805843No Hit
GCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGA1186440.1688664321944784No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG1185160.16868424933381207No Hit
ACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATACG1134170.16142682428273789No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG1115590.15878232619587854No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1110680.1580834841287914No Hit
GGTGACTCCAAAATTTTCAGATTCCAAAACTCCAAATCTGCTTCTTTGTT1091060.15529096246764065No Hit
CCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGA1086270.15460920004374096No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT1078440.1534947533257588No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1071730.15253971661085963No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT1071050.15244293196613065No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1041590.14824987956360772No Hit
GGGTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTT1040900.14815167161527978No Hit
ACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACGAC1030090.14661308042480886No Hit
CTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTT987630.14056973334364373No Hit
ATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATG967760.13774162909251914No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC949310.1351156339524462No Hit
CGACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATA942540.13415205741595332No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA941340.13398126098407864No Hit
GTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATA902410.12844034007334484No Hit
CCTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTG894190.1272703845150034No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA889860.12665409405665565No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG841530.11977526776290363No Hit
CCTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCC833720.11866366765211937No Hit
CGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACG822790.117107996818461No Hit
GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT815860.11612164742438483No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT810320.11533313723056347No Hit
CTTAACGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGC807570.1149417287408507No Hit
CGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAAGAC804130.11445211230281No Hit
CTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCT794020.11301315236426597No Hit
GCAAAGACCAATCACTGGTGCTTCTTTGGACTTGATCAAGGAAAGAAGAT783030.11144894170901387No Hit
CTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGC779680.11097213500336377No Hit
TGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAAC770050.10960149363756959No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT760540.10824793191496289No Hit
CTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACGACT754660.10741102939877706No Hit
CTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATACGA747790.10643321982629463No Hit
GCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATA746710.10627950303760743No Hit
AAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCAC739270.10522056515998451No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA724830.10316531476309275No Hit
GCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAA714740.10172920143174664No Hit
AAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAA704600.10028597158240574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCT101950.034.9355621
TCACGGT1723450.034.08362
CGGGTGC78900.033.0197371
ATCACGG1891150.031.2555581
CACGGTC2006400.029.1393283
CGCCAAC53500.028.4915831
CTCCGCT45850.025.9661431
GTCGCCA56900.023.37481
TGGCGCT142450.022.9398351
TCGCCAC60000.022.329762
GTCCGTA24800.021.4922071
GATCTAC292950.021.16995
CGGGTAT33000.020.2273881
CCCCGTT74900.020.2181151
ATCTACC307750.020.1922666
CACGACT59450.020.1098231
CGTCACT71350.019.9673751
CTTTTAT4675350.019.8920291
TCTACCC313650.019.8903737
TTATATA4684650.019.5592634
Produced by FastQC (version 0.11.5)