FastQCFastQC Report
Fri 27 May 2016
SRR1233110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70259079
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC9552811.3596548853138255No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG6198790.882276011616947No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG5760880.819948123715086No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA5051130.7189291507792182No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG4540610.6462666554453411No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA3675660.5231580106536836No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA3665170.5216649651783793No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT3626530.5161653200720152No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA3617550.5148871934401531No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC3499610.49810075079407173No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT3371330.47984261222667035No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG3052190.434419301169604No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC3030410.4313193459310789No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC2768660.3940643742284183No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT2757360.3924560411615985No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT2584450.3678456986320586No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG2394620.34082712641308605No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA2301950.3276373719615653No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA2293900.32649161256440606No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA2240890.3189466801863429No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT2168110.3085878765931446No Hit
ATACGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAAT2101820.2991527970356685No Hit
ATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGT2089760.29743629289532814No Hit
GGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAAGT2065440.2939748185426683No Hit
CGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGAA2041280.2905361170475918No Hit
GAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACA1722620.24518112456327532No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC1715550.24417484891881375No Hit
ATACGACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTA1689630.24048564599032105No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT1685860.2399490605335148No Hit
TAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTT1680530.23919043971527154No Hit
ATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAA1648380.23461451864462954No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT1592520.22666394474086402No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA1504710.2141659158384356No Hit
ATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTAT1501130.21365637315000954No Hit
GGCAATTGTAATACGACTCACTATAGGCAATTGTAATACGACTCACTATA1474530.2098703855767879No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1459100.20767422812359954No Hit
GCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGAAACCTTGGA1450070.2063889849737427No Hit
CGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTAC1421670.202346802752709No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG1407970.20039687682213997No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG1385670.19722290979646914No Hit
CAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGAA1343220.191180986018903No Hit
GTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATA1331830.18955984321969266No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC1310300.18649547057114144No Hit
CCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGG1255840.17874415917122966No Hit
ACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATACG1247140.17750588504013837No Hit
CCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGA1246570.1774247567349979No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT1238490.17627472742704184No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1216600.17315911584892824No Hit
CTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTT1216370.17312637986615223No Hit
CTTAACGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGC1151360.16387348316934242No Hit
ATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATG1131280.16101548954263975No Hit
GAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCC1128120.1605657256053698No Hit
ACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACGAC1123750.15994374193262625No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA1103280.1570302394655643No Hit
GGGTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTT1078310.1534762503789724No Hit
CCTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTG1068460.15207429633400119No Hit
CGACTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATA1066520.15179817543580382No Hit
CTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTA1060830.15098831568799814No Hit
GGTGACTCCAAAATTTTCAGATTCCAAAACTCCAAATCTGCTTCTTTGTT1051480.14965752682297473No Hit
AAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTTT1035490.14738166436824485No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG1031390.14679810989267308No Hit
GTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATA1009010.14361275643821064No Hit
CCTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCC993240.14136820666265779No Hit
CGAAGAAGTTGCTAAGAAGAGATCTAGAAAGACCGTTAAGGCGCAAAGAC983000.13991074377732735No Hit
AATTATCTTAACGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCT941570.13401399696685465No Hit
TGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAAC921010.1310876847674021No Hit
CGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACG916130.1303931126111118No Hit
CTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCT916060.13038314948591911No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT902560.12846168962732915No Hit
AAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAA899370.12800765577926235No Hit
CTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAATACGACT897770.12777992720342946No Hit
CTATAGTGAGTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGC895410.12744402755407597No Hit
CGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTTGTTGCTTGGA887560.1263267342288959No Hit
CTCACTATAGGCAATTGTAATACGACTCACTATAGGCAATTGTAATACGA886410.126163054315016No Hit
GCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAA850190.12100784867959911No Hit
GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT849900.12096657287522941No Hit
AAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCAC839010.11941659525596686No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT835030.11885012042358255No Hit
CCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAA822110.11701121217373202No Hit
ATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCA819520.11664257654160255No Hit
GCAAAGACCAATCACTGGTGCTTCTTTGGACTTGATCAAGGAAAGAAGAT806200.11474673614779379No Hit
GTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGTCGT798520.11365363898379595No Hit
AAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGGCTAA791980.11272279843007905No Hit
TATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTT790380.11249506985424618No Hit
GGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTAT783590.11152864671055537No Hit
GCCGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATA779570.11095647866377525No Hit
TAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGCAACCTT776500.11051952445889592No Hit
ATTATCTTAACGGGAAGTGGCGGCAACCTTTTGGAAAGCACCCTTAGCTT775420.11036580767020872No Hit
CAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTTTTCTTGT773270.11005979739643329No Hit
TATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAA760030.10817534343141616No Hit
GCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCA735620.10470105934636574No Hit
TTTTTTTTCTATTGTATCACGGTCTTTTATATATTATATTATTTGATACT724030.10305145047517632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCT97050.034.188641
TCACGGT1791000.033.414982
CGGGTGC72700.032.7401161
ATCACGG1919700.031.2624511
GTCCGTA23300.030.8507421
CGCCAAC52250.030.4301991
CTCCGCT45800.028.4793241
CACGGTC2131000.028.101053
TGGCGCT121600.025.2113971
GTCGCCA48500.024.538211
GCGACTC73000.023.9976981
CCCCGTT69900.023.699911
CGTCACT73750.022.9790781
CGGCTGA63050.022.9526631
CGGGTAT35200.022.5849131
GCCCGTT38650.022.17011
GATCTAC303500.020.8837645
TCGCCAC57550.020.573262
TTGTATC839400.020.2627812
CTTTTAT4913900.020.2626821
Produced by FastQC (version 0.11.5)