FastQCFastQC Report
Fri 27 May 2016
SRR1233109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38585565
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1946430.5044451208632036No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1624770.42108233998906064No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1154720.29926217226571644No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1100750.2852750763141605No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1091680.2829244563349014No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1074690.2785212552932684No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG702710.1821173280733352No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA681490.17661786214611605No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT664280.1721576449638615No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA626930.16247785927198422No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT609140.15786732680990936No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC597600.15487657107003616No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT588670.1525622340893544No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT573230.14856073767482736No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC562740.14584210442428405No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC478630.12404379720758268No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT476900.12359544301087727No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA473570.1227324259732882No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG411460.10663573281873674No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG408770.10593858091750115No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT403570.10459092668462934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT488350.029.9629082
CGGCCAG184000.025.8718591
CACGGTC577250.025.1939413
ATCACGG661250.022.7251361
TTACGCG70000.020.4734848
TACGCGC73850.019.4282919
CGCCGTA40900.018.9281446-47
CGCTTCG27450.018.1956796
CGGGTGC55900.017.7263551
GTCGCCG43050.017.09861244-45
CTTTTAT1543800.017.0886921
CGGTGCT58700.016.8808041
TATTATA1478900.016.7462089
TTATATA1473300.016.5863534
ACGGTCT874900.016.4947154
CGCCAAC33550.015.9855031
CGCGCAG79650.015.76302210-11
TCGCCGT44250.015.67051446-47
CGGGTAT30300.015.50867751
CGACCGT9600.014.8976011
Produced by FastQC (version 0.11.5)