##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233109_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38585565
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.374132450827144	31.0	26.0	31.0	2.0	34.0
2	26.405973995715755	31.0	26.0	31.0	2.0	34.0
3	26.55918870178524	31.0	26.0	31.0	10.0	34.0
4	29.93785448003677	35.0	28.0	37.0	10.0	37.0
5	29.815661582252325	35.0	28.0	37.0	10.0	37.0
6	30.189442217575408	35.0	30.0	37.0	10.0	37.0
7	29.518628948416332	35.0	30.0	37.0	2.0	37.0
8	29.62379687844405	35.0	29.0	37.0	7.0	37.0
9	31.108771039117865	35.0	30.0	39.0	10.0	39.0
10-11	31.360937322027034	35.0	30.0	39.0	10.5	39.0
12-13	31.229297575401578	35.0	30.0	39.0	9.0	39.0
14-15	31.438336279383236	37.0	29.5	40.0	5.0	41.0
16-17	31.53513921592181	37.0	29.5	40.0	5.0	41.0
18-19	31.541795902172225	37.0	29.0	40.0	8.0	41.0
20-21	31.524084226834567	37.0	29.5	40.0	7.5	41.0
22-23	31.70682478019954	37.0	30.0	40.0	8.0	41.0
24-25	31.44194534925172	37.0	29.5	40.0	4.5	41.0
26-27	31.322066879155457	36.5	29.5	40.0	2.0	41.0
28-29	31.01866166790612	36.0	29.0	40.0	2.0	41.0
30-31	30.88172242650846	36.0	29.0	40.0	2.0	41.0
32-33	30.686738887975334	36.0	28.5	40.0	2.0	41.0
34-35	30.49526164771722	36.0	28.0	40.0	2.0	41.0
36-37	30.423740536130545	35.5	28.0	40.0	2.0	41.0
38-39	30.216557707526118	35.0	27.5	39.5	2.0	41.0
40-41	29.955574008051975	35.0	27.0	39.0	2.0	41.0
42-43	29.784750942483285	35.0	27.0	39.0	2.0	40.0
44-45	29.404455487434227	35.0	26.0	39.0	2.0	40.0
46-47	29.42476414431148	35.0	26.5	39.0	2.0	40.0
48-49	29.55264292488655	35.0	27.0	39.0	2.0	40.0
50-51	29.024698342501917	34.0	26.0	38.5	2.0	40.5
52-53	29.088289843624164	34.5	26.0	39.0	2.0	40.0
54-55	29.264145166722322	35.0	26.0	39.0	2.0	41.0
56-57	29.30268565459648	35.0	26.0	40.0	2.0	41.0
58-59	29.144458828061737	35.0	26.0	39.0	2.0	41.0
60-61	28.818283210314533	34.0	26.0	39.0	2.0	41.0
62-63	28.532360249227917	34.0	25.5	39.0	2.0	40.5
64-65	28.220094781558856	34.0	25.0	39.0	2.0	40.0
66-67	27.88839912024095	34.0	23.5	38.0	2.0	40.0
68-69	27.553754001010482	34.0	23.0	37.5	2.0	40.0
70-71	27.09370934441416	33.0	22.0	37.0	2.0	40.0
72-73	26.642295855976194	33.0	20.5	36.5	2.0	39.0
74-75	26.27415994815678	32.5	20.0	36.0	2.0	39.0
76-77	25.848963504875464	32.0	18.0	36.0	2.0	38.5
78-79	25.473014778972395	32.0	15.5	35.0	2.0	37.5
80-81	25.068754208989812	32.0	11.0	35.0	2.0	37.0
82-83	24.74860750386835	32.0	7.5	35.0	2.0	36.5
84-85	24.444467911769596	32.0	4.5	35.0	2.0	36.0
86-87	24.096905137970637	31.0	2.0	35.0	2.0	36.0
88-89	23.68736286484337	31.0	2.0	35.0	2.0	35.0
90-91	23.387527822904758	31.0	2.0	34.0	2.0	35.0
92-93	23.111484035545416	31.0	2.0	34.0	2.0	35.0
94-95	22.79743567315912	31.0	2.0	34.0	2.0	35.0
96-97	22.476883220447853	30.5	2.0	34.0	2.0	35.0
98-99	22.011402113717914	30.0	2.0	34.0	2.0	35.0
100-101	20.43763421631898	27.5	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.236656416448774
1101	2	5.271625949402978
1101	3	5.084475502177824
1101	4	5.4709916528965685
1101	5	5.527921565106627
1101	6	5.023806849121215
1101	7	6.308346843327559
1101	8	6.017392710142499
1101	9	5.836348958177737
1101	10-11	5.324185309524548
1101	12-13	5.392727429318455
1101	14-15	6.46035223324656
1101	16-17	6.075733197748772
1101	18-19	5.953280046714049
1101	20-21	5.90716561863168
1101	22-23	5.472453028195552
1101	24-25	5.550731556318453
1101	26-27	5.515071442608605
1101	28-29	5.5962001551467395
1101	30-31	5.626277896808748
1101	32-33	5.714357374519331
1101	34-35	5.678743019135673
1101	36-37	5.440758810132085
1101	38-39	5.51728268038654
1101	40-41	5.625547608872875
1101	42-43	5.595841606320754
1101	44-45	5.642979176122012
1101	46-47	5.538004345375533
1101	48-49	5.425286310800306
1101	50-51	5.579053914096537
1101	52-53	5.620487533191845
1101	54-55	5.868527304518132
1101	56-57	5.805351519943134
1101	58-59	5.58735536323271
1101	60-61	5.572877803025403
1101	62-63	5.672216496551528
1101	64-65	5.645875281871767
1101	66-67	5.647853083945897
1101	68-69	5.477168388794862
1101	70-71	5.433138266986379
1101	72-73	5.4502380692832055
1101	74-75	5.412644517450648
1101	76-77	5.3239598457231985
1101	78-79	5.271727382370543
1101	80-81	5.328802911341036
1101	82-83	5.272813486498787
1101	84-85	5.312499565134253
1101	86-87	5.199429667957979
1101	88-89	5.125622221563045
1101	90-91	5.0528372159840345
1101	92-93	5.060091959976081
1101	94-95	5.0213287957151245
1101	96-97	4.885251913636601
1101	98-99	4.828616557968736
1101	100-101	4.4198056451753125
1102	1	5.01783266857851
1102	2	5.186264123187268
1102	3	4.832715923977727
1102	4	5.182262818803945
1102	5	5.2618243162003075
1102	6	4.819768117720997
1102	7	6.0931791349383
1102	8	5.867623476000812
1102	9	5.60169116592354
1102	10-11	5.100157658006136
1102	12-13	5.191819408281752
1102	14-15	6.234900549757796
1102	16-17	5.9486857099573704
1102	18-19	5.75013466987896
1102	20-21	5.721610997168568
1102	22-23	5.25241310292062
1102	24-25	5.389629738851454
1102	26-27	5.3121858434997655
1102	28-29	5.35419888522344
1102	30-31	5.298739022847332
1102	32-33	5.414649122790429
1102	34-35	5.368930503994193
1102	36-37	5.156920824624802
1102	38-39	5.373035685118996
1102	40-41	5.408946886284088
1102	42-43	5.377944580770169
1102	44-45	5.5330939539972235
1102	46-47	5.351604865857595
1102	48-49	5.256476924372858
1102	50-51	5.310984333146042
1102	52-53	5.360062618902074
1102	54-55	5.647260031454671
1102	56-57	5.569878014250111
1102	58-59	5.484123792941617
1102	60-61	5.509638779704343
1102	62-63	5.546107553971424
1102	64-65	5.496033779107101
1102	66-67	5.465752330327621
1102	68-69	5.4281503376593285
1102	70-71	5.40483462216163
1102	72-73	5.4020979042095725
1102	74-75	5.324501152886384
1102	76-77	5.2371795253190605
1102	78-79	5.112531264719486
1102	80-81	5.054564037540967
1102	82-83	5.149463247560025
1102	84-85	5.123995767143924
1102	86-87	5.111270762535511
1102	88-89	5.077540397945349
1102	90-91	5.045369548427789
1102	92-93	4.964621642687025
1102	94-95	4.808348259692551
1102	96-97	4.691175833528213
1102	98-99	4.571969984961374
1102	100-101	4.232887864746896
1103	1	4.952470583202857
1103	2	5.114247117812589
1103	3	4.99217985707746
1103	4	5.410878889520756
1103	5	5.526167765518441
1103	6	5.032852385487672
1103	7	6.3602830624813045
1103	8	6.028920651201833
1103	9	5.986496341716318
1103	10-11	5.548655553345068
1103	12-13	5.6225188573917535
1103	14-15	6.593858228384242
1103	16-17	6.290232754798925
1103	18-19	5.9283701845915076
1103	20-21	6.032901187833886
1103	22-23	5.6573559928702934
1103	24-25	5.833285194254302
1103	26-27	5.684485809857822
1103	28-29	5.7855811719588885
1103	30-31	5.65503649714282
1103	32-33	5.8525852553678845
1103	34-35	5.778807025251098
1103	36-37	5.775096347721874
1103	38-39	5.766371002837044
1103	40-41	5.785502895926303
1103	42-43	5.7149867792925235
1103	44-45	5.803762769406376
1103	46-47	5.678694280212071
1103	48-49	5.744409412508876
1103	50-51	5.801413221951986
1103	52-53	5.958973066865266
1103	54-55	6.225222318113488
1103	56-57	6.119172424742931
1103	58-59	6.021363366007748
1103	60-61	5.937592963312998
1103	62-63	5.971729305876515
1103	64-65	5.942632117105852
1103	66-67	6.034075528230897
1103	68-69	5.950092280517698
1103	70-71	5.868973863801411
1103	72-73	5.816858213359556
1103	74-75	5.825072468132081
1103	76-77	5.662571612527593
1103	78-79	5.630645057868961
1103	80-81	5.671657302118824
1103	82-83	5.709951111122027
1103	84-85	5.7374885012493095
1103	86-87	5.708327890753978
1103	88-89	5.676106031384801
1103	90-91	5.628138326524287
1103	92-93	5.618917773282053
1103	94-95	5.433005020976481
1103	96-97	5.312660827698959
1103	98-99	5.158600279721448
1103	100-101	4.869138612328591
1104	1	4.222767758137866
1104	2	4.358673123195199
1104	3	3.5330066676906853
1104	4	4.39891076739443
1104	5	2.800453371606089
1104	6	3.827192073908911
1104	7	4.290784161636694
1104	8	4.084336206844743
1104	9	4.5294308663708165
1104	10-11	4.391003400400717
1104	12-13	4.353696947206238
1104	14-15	5.408368924743833
1104	16-17	4.8891047219971036
1104	18-19	4.0867453432436776
1104	20-21	2.2785387884952897
1104	22-23	3.074324822873308
1104	24-25	4.358336102544563
1104	26-27	4.446321418223246
1104	28-29	4.621858923211761
1104	30-31	4.600221563917909
1104	32-33	4.6528215698089355
1104	34-35	4.550079796467145
1104	36-37	4.38054864605785
1104	38-39	4.394855123215162
1104	40-41	4.382710830076039
1104	42-43	4.431064577943168
1104	44-45	4.5908133599568615
1104	46-47	4.396363749767659
1104	48-49	4.242022520991743
1104	50-51	4.358999636578215
1104	52-53	4.487810556304847
1104	54-55	4.6728259199159865
1104	56-57	4.390494119343277
1104	58-59	4.4352009931392935
1104	60-61	4.519525090811339
1104	62-63	4.593825871609887
1104	64-65	4.521458639407776
1104	66-67	4.494829240117941
1104	68-69	4.470200129967363
1104	70-71	4.477628976119934
1104	72-73	4.414615051257396
1104	74-75	4.20845827476942
1104	76-77	4.248228468002534
1104	78-79	4.262367414608647
1104	80-81	4.128567938026833
1104	82-83	4.179639223918205
1104	84-85	4.2047030777059184
1104	86-87	4.239224914948373
1104	88-89	4.306701244724394
1104	90-91	4.183669119602012
1104	92-93	3.9660896483604695
1104	94-95	3.8775851142803504
1104	96-97	3.810197020107964
1104	98-99	3.7194598773430165
1104	100-101	3.5699713784338094
1105	1	0.38052349910865146
1105	2	-0.3631832148293199
1105	3	1.3812703384671003
1105	4	1.1299705788212897
1105	5	1.3921868906404242
1105	6	-1.8656023985867058
1105	7	0.21876700580007835
1105	8	1.9074473544515698
1105	9	-0.35534283686640933
1105	10-11	-2.1444975212786694
1105	12-13	-2.7546860346445072
1105	14-15	-1.5074978007562692
1105	16-17	-0.6542337486644776
1105	18-19	-0.5084561196718695
1105	20-21	-1.7176994706711746
1105	22-23	-2.3779052922975623
1105	24-25	-2.5743797884891535
1105	26-27	-1.7959938376897853
1105	28-29	-1.4336428081100934
1105	30-31	-1.3994169686421039
1105	32-33	-1.9982503996776586
1105	34-35	-1.0337567966759345
1105	36-37	-1.9902712097248134
1105	38-39	-2.4457780059660728
1105	40-41	-2.3392008959099435
1105	42-43	-2.100942786594171
1105	44-45	-2.109721935776882
1105	46-47	-2.028924688397815
1105	48-49	-1.620235751617983
1105	50-51	-0.0856565325787777
1105	52-53	-1.2759737161404452
1105	54-55	-0.7287801897286812
1105	56-57	0.31054241233939806
1105	58-59	-1.4881150399388403
1105	60-61	-1.372574054033155
1105	62-63	-1.5673543084062977
1105	64-65	-0.6359995805929763
1105	66-67	-0.7903342505349613
1105	68-69	-1.3046273060945168
1105	70-71	-1.153079819289868
1105	72-73	-0.9269456619834706
1105	74-75	-1.1490566631791914
1105	76-77	-1.6624475570699495
1105	78-79	-1.2948470837111614
1105	80-81	-0.9147318676964353
1105	82-83	-0.9274547142814065
1105	84-85	-0.7842714681676846
1105	86-87	-1.0635999025500098
1105	88-89	-0.8923881296238392
1105	90-91	-1.016291979412209
1105	92-93	-0.9183396036443447
1105	94-95	-0.9988897658252398
1105	96-97	-0.6216950036895312
1105	98-99	-0.5758962391516356
1105	100-101	-1.1431860925070083
1106	1	2.3493183624598117
1106	2	2.2634514007362903
1106	3	3.0353456175524833
1106	4	1.90799835557063
1106	5	0.39754302169529154
1106	6	0.9240680429611707
1106	7	3.7339073948188357
1106	8	2.649232346532621
1106	9	1.7022746411243332
1106	10-11	2.254705256053793
1106	12-13	2.666080102697194
1106	14-15	2.8641644168419234
1106	16-17	3.4236899504778044
1106	18-19	1.527303221001592
1106	20-21	1.3851336928091342
1106	22-23	2.215584761210877
1106	24-25	2.9894724351288318
1106	26-27	2.1106064752839195
1106	28-29	3.232387983661397
1106	30-31	2.407243247228127
1106	32-33	1.6802925060768494
1106	34-35	0.8944358664223095
1106	36-37	1.7936089780757918
1106	38-39	3.024554324819956
1106	40-41	3.106930725703492
1106	42-43	2.4303566515266226
1106	44-45	1.9901423229926607
1106	46-47	3.119998495664504
1106	48-49	3.33303105672708
1106	50-51	3.383801726097669
1106	52-53	3.484518454398785
1106	54-55	3.540943868192432
1106	56-57	3.467309995296805
1106	58-59	3.3619375626233
1106	60-61	3.2160373791040406
1106	62-63	3.388332475485555
1106	64-65	3.4636885137992195
1106	66-67	3.445022782937734
1106	68-69	3.2114177254845124
1106	70-71	3.195376478038856
1106	72-73	3.1131905218523563
1106	74-75	3.109788378861193
1106	76-77	3.128928193840668
1106	78-79	3.0113285087470985
1106	80-81	2.9942082141881627
1106	82-83	2.94966291266352
1106	84-85	2.988984907539539
1106	86-87	2.9826652388376473
1106	88-89	2.998850506275687
1106	90-91	2.880302753754986
1106	92-93	2.8333814418461323
1106	94-95	2.679200543997503
1106	96-97	2.576900546439653
1106	98-99	2.5946225107408125
1106	100-101	2.5887252716762923
1107	1	1.1019521455772718
1107	2	0.37262017926170543
1107	3	-0.9549839750150859
1107	4	-0.10844395808092955
1107	5	-0.17760609672550132
1107	6	0.14675288734644099
1107	7	0.6693455763589888
1107	8	0.5058314219329283
1107	9	0.6991568133939481
1107	10-11	0.683429688067779
1107	12-13	0.8337707054229639
1107	14-15	-1.058452553597725
1107	16-17	-1.3147393636800508
1107	18-19	-0.8870761280895074
1107	20-21	-2.339081888659358
1107	22-23	-1.013423823594394
1107	24-25	-3.2356952988959975
1107	26-27	-4.530354880080441
1107	28-29	-2.826263858148934
1107	30-31	-1.8460650471331412
1107	32-33	-1.1093473525417181
1107	34-35	-2.213338132404864
1107	36-37	-0.4807708888184479
1107	38-39	-1.5320041510601001
1107	40-41	-0.15669044321893466
1107	42-43	-0.07504154540114172
1107	44-45	-0.5994994012988748
1107	46-47	-0.8319933211194694
1107	48-49	0.43415223917851975
1107	50-51	0.5523730377393257
1107	52-53	0.6133214521435093
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2208	2	2.8466680082537827
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2208	4	2.9731656040807763
2208	5	3.772355374492374
2208	6	0.7417166287947445
2208	7	3.7033213523354185
2208	8	3.692226327226461
2208	9	3.603930957355594
2208	10-11	2.6558935882829395
2208	12-13	0.5810067819762708
2208	14-15	3.019722295125952
2208	16-17	2.6852649365950363
2208	18-19	0.5904735815435416
2208	20-21	3.075990205744233
2208	22-23	2.895940724932359
2208	24-25	2.8618911693365163
2208	26-27	3.202875444091635
2208	28-29	3.048010228548087
2208	30-31	2.818275126609368
2208	32-33	1.9070581249964391
2208	34-35	2.2822540639189057
2208	36-37	2.2051301986124265
2208	38-39	2.2603626835737636
2208	40-41	2.6041697929555916
2208	42-43	1.9454212335400207
2208	44-45	2.3066577723307375
2208	46-47	2.1768823747407566
2208	48-49	2.266432203234789
2208	50-51	1.5910800858752694
2208	52-53	2.695679433150108
2208	54-55	2.4951716314397565
2208	56-57	2.899852753154505
2208	58-59	2.2781140529573563
2208	60-61	2.0990347229523643
2208	62-63	2.174039918834204
2208	64-65	2.0910773625317063
2208	66-67	2.2759016279020408
2208	68-69	2.2571681374114725
2208	70-71	1.753063890065512
2208	72-73	2.6420529870248295
2208	74-75	2.5703693600299395
2208	76-77	2.726925921274198
2208	78-79	1.8414301460964637
2208	80-81	2.4182836760180493
2208	82-83	2.471426025046412
2208	84-85	2.1990018526254147
2208	86-87	2.6197280471798514
2208	88-89	2.7591618260392856
2208	90-91	2.819309810114742
2208	92-93	2.6164693841651
2208	94-95	2.6149633998305717
2208	96-97	2.3541006216692537
2208	98-99	2.306043751217178
2208	100-101	1.813082889403784
2209	1	3.206295662969673
2209	2	2.752981220967868
2209	3	3.3855593944366333
2209	4	3.331211813297955
2209	5	3.542893159182004
2209	6	3.3684112722472435
2209	7	4.823392503999923
2209	8	4.609692364170304
2209	9	4.514162767164741
2209	10-11	3.462543559582034
2209	12-13	3.59513353797405
2209	14-15	4.993952801514077
2209	16-17	4.722363615108652
2209	18-19	4.590475654439324
2209	20-21	2.2436370428550703
2209	22-23	0.09414932905744422
2209	24-25	2.812294144613759
2209	26-27	3.499023012302498
2209	28-29	3.9005805033451395
2209	30-31	3.8874948508279594
2209	32-33	2.9454033856127566
2209	34-35	3.3614277747540484
2209	36-37	3.2780828602162693
2209	38-39	4.1100605513527135
2209	40-41	4.15063331630704
2209	42-43	2.5257220174595147
2209	44-45	3.4529973707405333
2209	46-47	3.8972808072789142
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2313	38-39	-26.600025574001993
2313	40-41	-26.326578313105014
2313	42-43	-26.137107175550888
2313	44-45	-25.77753730342684
2313	46-47	-25.776886564653182
2313	48-49	-25.917526130098548
2313	50-51	-25.404440747458064
2313	52-53	-25.421025112304438
2313	54-55	-25.571864353408728
2313	56-57	-25.61310179230004
2313	58-59	-25.495351856133553
2313	60-61	-25.201145201290057
2313	62-63	-24.89831803571125
2313	64-65	-24.595532485953143
2313	66-67	-24.2552726145222
2313	68-69	-23.931089438346103
2313	70-71	-23.47746641296479
2313	72-73	-23.045068225096383
2313	74-75	-22.69529351875794
2313	76-77	-22.282903624180346
2313	78-79	-21.925700535721717
2313	80-81	-21.536773142163767
2313	82-83	-21.221358065862677
2313	84-85	-20.917655344344283
2313	86-87	-20.579845816456512
2313	88-89	-20.181368476991018
2313	90-91	-19.902925038370107
2313	92-93	-19.645339327956112
2313	94-95	-19.344307433502763
2313	96-97	-19.050084984391354
2313	98-99	-18.60838806696464
2313	100-101	-17.149379231808336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2842461.0
3	253371.0
4	205712.0
5	195437.0
6	222180.0
7	225747.0
8	278116.0
9	313574.0
10	284910.0
11	295927.0
12	296793.0
13	312483.0
14	340383.0
15	374142.0
16	398697.0
17	414456.0
18	418426.0
19	421123.0
20	417105.0
21	425987.0
22	450367.0
23	500695.0
24	609166.0
25	761031.0
26	926039.0
27	1016099.0
28	1065856.0
29	1160490.0
30	1320764.0
31	1491060.0
32	1714112.0
33	2015811.0
34	2383072.0
35	2844508.0
36	3445385.0
37	4213134.0
38	3245647.0
39	484833.0
40	466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.92266773427613	18.694667283458788	15.644147795916924	30.738517186348158
2	21.037314232177287	20.650297436890746	40.107070700423996	18.20531763050797
3	23.492560097683654	23.380982957386472	30.30951585654608	22.816941088383793
4	23.83501781263154	29.84028112897894	26.693624252122838	19.631076806266684
5	23.29431420588026	32.40924406881195	28.321220894766075	15.975220830541717
6	18.64581956129679	37.27198291538532	27.178767305321394	16.903430217996494
7	16.829091194756806	19.695713532197605	45.896875631270625	17.578319641774964
8	18.172256418533625	24.517839204936315	36.422603086546445	20.887301289983615
9	18.42896908358003	24.52257388756905	35.80076992003226	21.24768710881866
10-11	19.678138236438762	34.80967079695137	27.520210389682052	17.99198057692781
12-13	19.548703420312343	28.710194093090337	33.61029442360932	18.130808062988002
14-15	20.61590489235399	28.28561000755724	32.991629854278735	18.106855245810042
16-17	20.680137154665907	28.269365099154903	32.223918960218256	18.826578785960937
18-19	20.041619394775083	29.410798394179306	31.2056414890369	19.34194072200871
20-21	20.737726233945917	29.90540395074207	29.913680530940546	19.443189284371474
22-23	20.665278175434594	29.462162376592122	31.341679674745155	18.53087977322813
24-25	20.14173413458203	28.995525080363848	31.86247339709329	19.000267387960832
26-27	20.321125410614247	29.433308561769252	30.676340250731364	19.569225776885137
28-29	20.994520790519676	28.26826950225933	30.207794455790605	20.52941525143039
30-31	19.901632029499368	29.21627450629415	30.664804166322174	20.21728929788431
32-33	19.91962981479711	29.104476251752025	31.26721816044973	19.708675773001136
34-35	19.56843519192799	31.627210691986164	29.338912791930266	19.465441324155577
36-37	20.163273378042195	30.211768005092143	29.53060302948312	20.09435558738254
38-39	20.15801480958847	29.96712933424553	30.6500523201997	19.224803535966302
40-41	20.784106655152822	30.150223960946636	30.06380502068991	19.001864363210625
42-43	20.10974778754359	29.850836711921215	31.242547518237934	18.796867982297268
44-45	20.201226766081245	30.216895251633503	30.24923549257212	19.332642489713134
46-47	20.609614997424742	29.476557860388276	30.73366833159033	19.18015881059665
48-49	21.28568574056957	29.60736849106963	30.33588889844386	18.77105686991694
50-51	20.464061427220344	29.63702164607655	30.121392024285093	19.777524902418012
52-53	20.019548221952494	29.238837173649085	30.58856785883118	20.153046745567245
54-55	19.868154536402905	29.30916394865819	30.935487424896923	19.88719409004198
56-57	19.642676457714288	29.48180999152453	30.604514308364244	20.270999242396936
58-59	20.139488956531814	29.540813962061446	30.538502048597582	19.78119503280916
60-61	19.745288707582855	29.42092689759693	31.018291026659455	19.815493368160748
62-63	19.541306245305485	29.32693308395234	31.491310265591395	19.640450405150784
64-65	19.77573230373933	29.827610687657685	30.812763884655027	19.583893123947963
66-67	19.164908544807766	30.52729580414393	30.535160211196743	19.772635439851562
68-69	19.508008189414497	30.07710588442738	31.582303289153597	18.83258263700453
70-71	19.864078329793134	30.39274576297379	30.694145662670852	19.049030244562225
72-73	19.759463399868864	30.597073755006814	30.173771247266107	19.469691597858212
74-75	19.065738898402547	29.296215656328457	31.846099225093795	19.791946220175205
76-77	19.24506912201641	29.751344576595613	31.397183189694257	19.606403111693723
78-79	19.247532049877197	30.561119239758362	30.082520208846823	20.10882850151762
80-81	19.52881179840535	31.555308395200054	29.828667504549156	19.08721230184544
82-83	20.020576329717553	29.979805502855978	30.66336557586864	19.33625259155783
84-85	19.65959382460396	30.708348680464592	30.44699228777669	19.185065207154757
86-87	20.4963350245541	31.465355058585164	28.856184273737167	19.18212564312357
88-89	20.255080406858827	30.576993112536844	29.893577225482243	19.274349255122083
90-91	20.465317082536295	30.991068492531515	29.354935999217098	19.188678425715093
92-93	20.067882185772955	30.721106928783275	29.910436544602252	19.300574340841514
94-95	20.05786826635999	31.686731157615473	29.538280836585535	18.717119739439006
96-97	20.03481287783342	31.27080481683415	29.67763558155671	19.016746723775714
98-99	19.929133400400264	31.06157164901392	29.797779970364978	19.211514980220834
100-101	21.045264977897833	31.624418865499077	28.471915381173357	18.858400775429732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3108.0
1	3638.0
2	5065.5
3	8457.0
4	13946.5
5	20069.0
6	25688.0
7	32280.5
8	40713.0
9	47312.5
10	58133.0
11	74382.0
12	85261.5
13	98975.5
14	112388.0
15	158801.5
16	298631.5
17	405505.0
18	494449.5
19	558478.5
20	568154.5
21	483376.5
22	322300.0
23	294754.0
24	381842.5
25	472077.0
26	508283.5
27	459049.0
28	430127.0
29	474058.5
30	538189.5
31	629562.5
32	746883.0
33	899115.5
34	1033291.5
35	1192185.5
36	1521347.0
37	1777562.5
38	1781673.5
39	1707593.0
40	1616391.5
41	1550472.5
42	1471797.0
43	1378077.0
44	1286860.5
45	1219242.5
46	1169690.5
47	1111901.0
48	1075869.0
49	1029786.5
50	949891.5
51	899568.0
52	850769.0
53	722546.5
54	589507.5
55	506200.0
56	439801.5
57	376477.0
58	320174.0
59	267488.0
60	218585.5
61	176044.5
62	138077.0
63	106332.5
64	80582.0
65	60364.5
66	44672.5
67	33780.5
68	25879.0
69	19150.0
70	14726.5
71	12180.0
72	10422.5
73	9395.0
74	8371.0
75	7040.0
76	5992.5
77	4852.0
78	3730.0
79	2667.5
80	1804.5
81	1165.0
82	694.0
83	423.0
84	288.5
85	203.0
86	146.0
87	92.0
88	58.0
89	43.5
90	34.5
91	25.0
92	23.5
93	18.5
94	10.5
95	10.0
96	8.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	7.352910343544276
2	7.8386204789278064
3	4.60521959442605
4	2.4669277228414304
5	3.879253809034544
6	1.812372061935597
7	7.627632250557949
8	6.357333370652989
9	3.2402246798770475
10-11	2.17202080622637
12-13	3.7418798973139307
14-15	5.188707227689941
16-17	5.179902121428053
18-19	3.227324778061433
20-21	3.227278128491834
22-23	1.9058111498432122
24-25	2.019086671401598
26-27	2.836098162615994
28-29	2.410572451122589
30-31	1.980030615075871
32-33	2.599343821970729
34-35	2.4125498745450535
36-37	1.5956446406836338
38-39	3.2261403973221596
40-41	2.4973173257926895
42-43	3.257634040087271
44-45	3.31096875217455
46-47	2.179031199880059
48-49	3.138953647562243
50-51	2.3253696038920255
52-53	2.831735132037071
54-55	2.212195156401105
56-57	1.8884510308453433
58-59	1.5766647449635636
60-61	1.9264924590322832
62-63	1.8855781948508465
64-65	1.2357522819738418
66-67	0.9400445477473247
68-69	0.7889997204913288
70-71	1.0527835992553174
72-73	0.23887560024065993
74-75	0.5095078949861171
76-77	1.0348105567457675
78-79	0.7841766733233011
80-81	0.7985395574744079
82-83	0.9042526654721785
84-85	0.8075105288726496
86-87	0.005938749374280252
88-89	0.4753059855414843
90-91	0.002687533537477033
92-93	0.28796131403025976
94-95	0.7461106245301837
96-97	0.00778399901621241
98-99	0.29943322068758094
100-101	0.2526514773076408
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8585565E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.16972461552335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57511666303789	33.97830480543975
2	9.336445020394288	7.874306307960029
3	3.542883897192021	4.48207314868079
4	1.7800422346587876	3.002555633582475
5	1.123581015730841	2.369055100829979
6	0.7051318223179057	1.7841128858872937
7	0.48067497066512826	1.4188951799766256
8	0.36920531925046496	1.245542931150279
9	0.2842058432866869	1.0786393930969955
>10	1.599868297011168	12.337422162328531
>50	0.11809255577930344	3.4237254633854803
>100	0.06943798581754301	5.6140412469886565
>500	0.007080384756663165	2.071879546950263
>1k	0.006224000724789643	5.55993889475665
>5k	0.0010981730042496298	3.3370764131824493
>10k+	9.118163723519952E-4	10.422430885803683
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC	194643	0.5044451208632036	No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG	162477	0.42108233998906064	No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG	115472	0.29926217226571644	No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA	110075	0.2852750763141605	No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA	109168	0.2829244563349014	No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG	107469	0.2785212552932684	No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG	70271	0.1821173280733352	No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA	68149	0.17661786214611605	No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT	66428	0.1721576449638615	No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA	62693	0.16247785927198422	No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT	60914	0.15786732680990936	No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC	59760	0.15487657107003616	No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT	58867	0.1525622340893544	No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT	57323	0.14856073767482736	No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC	56274	0.14584210442428405	No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC	47863	0.12404379720758268	No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT	47690	0.12359544301087727	No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA	47357	0.1227324259732882	No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG	41146	0.10663573281873674	No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG	40877	0.10593858091750115	No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT	40357	0.10459092668462934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.038913515974173245	0.0	0.0	7.774928266568081E-6	0.0
2	0.03892906583070638	7.774928266568081E-6	0.0	1.0366571022090774E-5	0.0
3	0.03895239061550609	7.774928266568081E-6	2.5916427555226935E-6	1.5549856533136162E-5	5.183285511045387E-6
4	0.03905346468297147	7.774928266568081E-6	2.5916427555226935E-6	1.8141499288658853E-5	5.183285511045387E-6
5	0.03910011425257088	7.774928266568081E-6	2.5916427555226935E-6	2.850807031074963E-5	5.183285511045387E-6
6	0.039136397251148196	7.774928266568081E-6	2.5916427555226935E-6	3.1099713066272324E-5	5.183285511045387E-6
7	0.03917527189248104	7.774928266568081E-6	2.5916427555226935E-6	3.3691355821795015E-5	5.183285511045387E-6
8	0.03926597938892433	1.0366571022090774E-5	2.5916427555226935E-6	3.3691355821795015E-5	5.183285511045387E-6
9	0.03954587680652078	1.0366571022090774E-5	2.5916427555226935E-6	4.1466284088363096E-5	5.183285511045387E-6
10-11	0.03981022436758409	1.0366571022090774E-5	2.5916427555226935E-6	4.6649569599408486E-5	5.183285511045387E-6
12-13	0.040290974098733556	1.1662392399852121E-5	2.5916427555226935E-6	4.794539097716983E-5	5.183285511045387E-6
14-15	0.040986830178591394	1.5549856533136162E-5	2.5916427555226935E-6	5.442449786597657E-5	5.183285511045387E-6
16-17	0.04198590846084539	1.5549856533136162E-5	2.5916427555226935E-6	5.701614062149926E-5	5.183285511045387E-6
18-19	0.04313659784429747	1.9437320666420202E-5	3.8874641332840405E-6	5.701614062149926E-5	5.183285511045387E-6
20-21	0.04419398808855073	2.3324784799704243E-5	5.183285511045387E-6	6.09036047547833E-5	5.183285511045387E-6
22-23	0.04538355211333565	2.3324784799704243E-5	6.479106888806734E-6	6.479106888806734E-5	7.774928266568081E-6
24-25	0.04651998746163235	2.5916427555226934E-5	7.774928266568081E-6	7.127017577687408E-5	7.774928266568081E-6
26-27	0.047831358695926834	2.5916427555226934E-5	7.774928266568081E-6	7.904510404344216E-5	7.774928266568081E-6
28-29	0.049374681956840596	2.850807031074963E-5	7.774928266568081E-6	8.03409254212035E-5	7.774928266568081E-6
30-31	0.05097761300113138	3.498717719955636E-5	7.774928266568081E-6	8.682003231001023E-5	7.774928266568081E-6
32-33	0.0525896147950665	4.1466284088363096E-5	9.070749644329427E-6	8.811585368777158E-5	1.0366571022090774E-5
34-35	0.054183475089712954	4.1466284088363096E-5	1.2958213777613467E-5	8.811585368777158E-5	1.0366571022090774E-5
36-37	0.05594320052071286	4.276210546612444E-5	1.2958213777613467E-5	9.459496057657832E-5	1.1662392399852121E-5
38-39	0.05789600333699921	4.405792684388579E-5	1.2958213777613467E-5	9.589078195433966E-5	1.2958213777613467E-5
40-41	0.06000300889723916	4.405792684388579E-5	1.2958213777613467E-5	1.0236988884314639E-4	1.2958213777613467E-5
42-43	0.06244822383707482	4.535374822164714E-5	1.2958213777613467E-5	1.0496153159866908E-4	1.2958213777613467E-5
44-45	0.06505282480637513	4.924121235493118E-5	1.5549856533136162E-5	1.1144063848747583E-4	1.2958213777613467E-5
46-47	0.06778182462794052	4.924121235493118E-5	1.5549856533136162E-5	1.1144063848747583E-4	1.2958213777613467E-5
48-49	0.07108487331985419	5.442449786597657E-5	1.5549856533136162E-5	1.1403228124299852E-4	1.2958213777613467E-5
50-51	0.07527167219140111	5.442449786597657E-5	1.5549856533136162E-5	1.1403228124299852E-4	1.2958213777613467E-5
52-53	0.08016210207107244	5.442449786597657E-5	1.5549856533136162E-5	1.1662392399852121E-4	1.2958213777613467E-5
54-55	0.08528318815598528	5.442449786597657E-5	1.5549856533136162E-5	1.192155667540439E-4	1.4254035155374815E-5
56-57	0.09109235539248939	5.442449786597657E-5	1.5549856533136162E-5	1.2180720950956659E-4	1.5549856533136162E-5
58-59	0.0975921954233403	5.572031924373791E-5	1.5549856533136162E-5	1.243988522650893E-4	1.5549856533136162E-5
60-61	0.1051520173412	5.701614062149926E-5	1.5549856533136162E-5	1.26990495020612E-4	1.5549856533136162E-5
62-63	0.11441454854943811	5.8311961999260604E-5	1.5549856533136162E-5	1.4254035155374814E-4	1.5549856533136162E-5
64-65	0.12579056442480496	6.219942613254465E-5	1.5549856533136162E-5	1.4513199430927083E-4	1.5549856533136162E-5
66-67	0.14018973157448905	6.219942613254465E-5	1.5549856533136162E-5	1.4642781568703216E-4	1.5549856533136162E-5
68-69	0.15912168190358233	6.3495247510306E-5	1.5549856533136162E-5	1.5290692257583893E-4	1.5549856533136162E-5
70-71	0.18270951844297212	6.479106888806734E-5	1.6845677910897508E-5	1.5420274395360026E-4	1.8141499288658853E-5
72-73	0.21458024522901245	6.479106888806734E-5	1.8141499288658853E-5	1.580902080868843E-4	1.8141499288658853E-5
74-75	0.25839585347525684	6.479106888806734E-5	1.8141499288658853E-5	1.5938602946464564E-4	1.8141499288658853E-5
76-77	0.31516190057084814	6.479106888806734E-5	1.8141499288658853E-5	1.8141499288658856E-4	1.8141499288658853E-5
78-79	0.39107759598699665	6.608689026582869E-5	1.8141499288658853E-5	1.8789409977539528E-4	1.8141499288658853E-5
80-81	0.4912186720603936	6.738271164359003E-5	1.8141499288658853E-5	1.9826067079748604E-4	2.0733142044181548E-5
82-83	0.6186212382791336	6.997435439911272E-5	1.8141499288658853E-5	2.0344395630853142E-4	2.0733142044181548E-5
84-85	0.7857161091200815	6.997435439911272E-5	1.8141499288658853E-5	2.0733142044181548E-4	2.3324784799704243E-5
86-87	0.9953735289349788	6.997435439911272E-5	2.0733142044181548E-5	2.138105273306222E-4	2.3324784799704243E-5
88-89	1.2520925895474124	7.256599715463541E-5	2.3324784799704243E-5	2.1510634870838355E-4	2.3324784799704243E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	48835	0.0	29.962908	2
CGGCCAG	18400	0.0	25.871859	1
CACGGTC	57725	0.0	25.193941	3
ATCACGG	66125	0.0	22.725136	1
TTACGCG	7000	0.0	20.473484	8
TACGCGC	7385	0.0	19.428291	9
CGCCGTA	4090	0.0	18.92814	46-47
CGCTTCG	2745	0.0	18.195679	6
CGGGTGC	5590	0.0	17.726355	1
GTCGCCG	4305	0.0	17.098612	44-45
CTTTTAT	154380	0.0	17.088692	1
CGGTGCT	5870	0.0	16.880804	1
TATTATA	147890	0.0	16.746208	9
TTATATA	147330	0.0	16.586353	4
ACGGTCT	87490	0.0	16.494715	4
CGCCAAC	3355	0.0	15.985503	1
CGCGCAG	7965	0.0	15.763022	10-11
TCGCCGT	4425	0.0	15.670514	46-47
CGGGTAT	3030	0.0	15.5086775	1
CGACCGT	960	0.0	14.897601	1
>>END_MODULE