FastQCFastQC Report
Fri 27 May 2016
SRR1233109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38585565
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC2508470.6501058102946011No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1674630.43400427076809683No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1440100.3732224732228231No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1374370.35618760539077243No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1372450.3556900099817121No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1080830.2801125239451593No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT888430.23024931732890264No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA846520.21938774254050705No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT808000.2094047346462336No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA727580.18856274360632014No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG711210.18432022441552948No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC695230.18017877929220422No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT691820.17929502911257097No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC690210.17887777462893184No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC650560.16860191110328435No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT622030.16120795432177812No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA593480.1538088142547608No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT580720.15050187809871385No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG520880.13499348784966606No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT470510.12193938329009826No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA460250.11928035782293198No Hit
ATACGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAAT445550.11547064297231362No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT442700.11473202478698963No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA434590.11263020251226073No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG428020.11092749322188233No Hit
CCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCC415920.10779160548769987No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT399390.10350762001282085No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG394180.10215737413719354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT541600.029.7987862
CGGCCAG198550.024.2794551
CACGGTC674050.023.9996033
ATCACGG710450.022.9919551
GTCGAAT58200.021.862411
GTCGGAT86200.021.8350351
CGGTGCT62000.020.2901231
CGGATTA88950.020.2013513
CGAATTA64700.020.07863
TTACGCG77100.019.492798
TATGCCG61450.019.01833748-49
TACGCGC80650.018.5759839
TCGGATT105400.017.768232
TATTATA1732450.017.3855029
CCGCTAC32050.017.3782121
CTTTTAT1774200.017.1713391
TTATATA1696950.016.8391154
CGTACGT69900.016.69123834-35
TAACGTA38700.016.6608478
CGGGTGC58350.016.3752271
Produced by FastQC (version 0.11.5)