FastQCFastQC Report
Fri 27 May 2016
SRR1233108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66292143
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1321530.19934941611406348No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1062330.16024975991498722No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1013610.15290047268497567No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT936120.14121130463379347No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG897600.1354006612819863No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT746560.11261666408943817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG350950.036.532451
CGACGGC159900.027.8985251
CACGGTC228500.025.140191
ACGGCCA237750.022.4045223
GACGGCC236850.022.331022
CGACGGA30300.021.7403411
CTGGAAT1856000.021.4726891
CGGCGAG57000.020.224241
GGCCAGT655550.019.38732
ACGGTCT304100.018.4438462
CTTTTAT1259250.018.2320921
TCACGGT210000.017.8748252
CGGTCTT353050.016.5758723
CGGACAG105350.016.505551
GCCAGTG783800.016.3410593
CGCGAGG26750.016.2962951
TTATATA993000.015.7424464
TATGGCG2567750.015.6133787
TATTATA981400.015.4656629
TGGCGAG2548500.015.3515839
Produced by FastQC (version 0.11.5)