FastQCFastQC Report
Fri 27 May 2016
SRR1233108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66292143
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1390600.20976844872853181No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1354350.20430022906334466No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC1078260.16265275961888878No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT1018560.1536471675082219No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG892740.13466754272825363No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT826990.1247493236113969No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA689190.10396254651173367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG330800.033.105221
CGACGGC162800.027.0238761
CACGGTC242850.024.1221521
ACGGCCA233950.022.25093
GACGGCC237450.021.8829922
CTGGAAT1921950.020.7302171
CGGCGAG56700.020.6968841
ACGGTCT318100.018.2425692
CGGACGT32050.017.1957451
CTTTTAT1271850.016.8249421
TCACGGT219000.016.734152
GGCCAGT654700.016.5394862
CGGTCTT370050.015.9890433
CGACGGA26000.015.7150651
TATGGCG2661850.015.5232377
TATTATA1040250.015.4584799
TGGCGAG2650500.015.3339369
ATGGCGA2722650.015.18180758
TTATATA1028000.014.5549994
CGGACAG89000.014.1464331
Produced by FastQC (version 0.11.5)