##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66292143 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.932639347622235 31.0 30.0 34.0 27.0 34.0 2 31.251474220708175 31.0 31.0 34.0 28.0 34.0 3 30.73905791520422 31.0 31.0 34.0 26.0 34.0 4 33.30004254048628 35.0 33.0 37.0 25.0 37.0 5 33.73921957840464 35.0 33.0 37.0 28.0 37.0 6 34.55700768339922 36.0 35.0 37.0 30.0 37.0 7 35.05675745012497 37.0 35.0 37.0 32.0 37.0 8 35.1529576891186 37.0 35.0 37.0 32.0 37.0 9 36.855849297253826 39.0 37.0 39.0 33.0 39.0 10-11 36.861775874404906 39.0 37.0 39.0 33.0 39.0 12-13 36.80668038593956 39.0 37.0 39.0 32.5 39.0 14-15 38.060069464038904 40.0 37.0 41.0 33.0 41.0 16-17 38.00963962652406 40.0 37.0 41.0 33.0 41.0 18-19 38.04033401062325 40.0 37.0 41.0 33.0 41.0 20-21 37.99665378142927 40.0 37.0 41.0 33.0 41.0 22-23 37.964944706946646 40.0 37.0 41.0 32.5 41.0 24-25 37.93838385945677 40.0 37.0 41.0 32.5 41.0 26-27 37.664778629950156 40.0 37.0 41.0 32.0 41.0 28-29 37.543732113170634 40.0 37.0 41.0 32.0 41.0 30-31 37.41126468034078 40.0 37.0 41.0 31.5 41.0 32-33 37.340407942461596 40.0 36.5 41.0 31.0 41.0 34-35 37.22710367803316 40.0 36.0 41.0 31.0 41.0 36-37 37.04523537578201 39.0 36.0 41.0 30.0 41.0 38-39 36.97187119897451 39.0 36.0 41.0 30.0 41.0 40-41 36.82598720635717 39.0 36.0 41.0 30.0 41.0 42-43 36.711718498827224 39.0 35.0 40.0 30.0 41.0 44-45 36.56288570275967 39.0 35.0 40.0 30.0 41.0 46-47 36.323391536761754 39.0 35.0 40.0 30.0 41.0 48-49 36.195803671635716 38.5 35.0 40.0 30.0 41.0 50-51 36.01601009489164 38.5 35.0 40.0 28.5 41.0 52-53 35.72580678980313 38.0 34.5 40.0 28.0 41.0 54-55 35.48746097256201 38.0 34.0 40.0 27.5 41.0 56-57 35.68231437321313 38.0 34.5 40.0 28.0 41.0 58-59 35.75837009824829 39.0 35.0 40.0 28.0 41.0 60-61 35.581309145489534 38.0 34.0 40.0 28.0 41.0 62-63 35.354923033639146 38.0 34.0 40.0 28.0 41.0 64-65 35.116387201421446 38.0 34.0 40.0 27.5 41.0 66-67 34.629991928304385 37.0 34.0 40.0 26.0 41.0 68-69 34.478788232867956 37.0 34.0 39.5 26.0 41.0 70-71 34.181305037913766 36.5 33.5 39.0 26.0 41.0 72-73 33.80518087942941 36.0 33.0 39.0 26.0 40.5 74-75 33.40400733462486 35.5 33.0 38.5 26.0 40.0 76-77 32.285688584543124 34.5 31.0 37.0 24.5 39.0 78-79 32.63693589600807 35.0 32.0 37.0 25.0 39.0 80-81 32.45308545086557 35.0 32.0 37.0 25.0 39.0 82-83 32.21362998477814 35.0 32.0 36.0 25.0 38.5 84-85 31.93333896295976 35.0 32.0 36.0 25.0 37.0 86-87 31.584149806410693 35.0 32.0 35.5 24.5 37.0 88-89 31.235565661227756 34.5 32.0 35.0 23.5 36.5 90-91 31.044795896853117 34.0 32.0 35.0 23.0 36.0 92-93 30.845360482614055 34.0 32.0 35.0 20.5 36.0 94-95 30.62646316924767 34.0 31.0 35.0 20.0 36.0 96-97 30.337917768022677 34.0 31.0 35.0 17.5 35.5 98-99 30.00015748472636 34.0 31.0 35.0 4.5 35.0 100-101 28.60118682541308 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3790335682895645 1101 2 1.3449724332078894 1101 3 1.9759927889656979 1101 4 2.9355546441516367 1101 5 2.511127100140179 1101 6 1.4025937587435209 1101 7 0.9024838302440372 1101 8 0.7255719358447479 1101 9 0.6943290919373339 1101 10-11 0.5472781528403914 1101 12-13 0.6717555633922387 1101 14-15 0.7627543077422061 1101 16-17 0.7185531102381759 1101 18-19 0.6859249135988534 1101 20-21 0.9912553447401109 1101 22-23 0.7929218291765565 1101 24-25 0.6517784195560381 1101 26-27 0.7159833767339805 1101 28-29 0.8341681905723277 1101 30-31 0.833130148914023 1101 32-33 0.897811892741359 1101 34-35 0.855062271647185 1101 36-37 0.854098568876573 1101 38-39 0.6589800072388599 1101 40-41 0.7498401137827955 1101 42-43 0.5938791995122585 1101 44-45 0.7125414684283768 1101 46-47 0.7178037116463258 1101 48-49 0.6367493623309457 1101 50-51 0.6102522000107342 1101 52-53 0.681556396787478 1101 54-55 0.662418860369371 1101 56-57 0.8367661826349106 1101 58-59 0.6948803537227874 1101 60-61 0.6672110363482489 1101 62-63 0.8087177065171147 1101 64-65 0.6962587809269181 1101 66-67 0.8842287727256348 1101 68-69 0.7134634023008246 1101 70-71 0.806343624909573 1101 72-73 0.7748733695886045 1101 74-75 0.6331868296088743 1101 76-77 0.6051922424079024 1101 78-79 0.6030999452639136 1101 80-81 0.6116062774756656 1101 82-83 0.5979564152435017 1101 84-85 0.548870035743608 1101 86-87 0.5081474462184126 1101 88-89 0.5446584300523227 1101 90-91 0.5258635513915486 1101 92-93 0.557036251523769 1101 94-95 0.5342549508841259 1101 96-97 0.4899091677087455 1101 98-99 0.5055123343065944 1101 100-101 0.3822299759483201 1102 1 1.362226947132143 1102 2 1.3272599906520988 1102 3 1.9324853972879694 1102 4 2.911589373837508 1102 5 2.5128115201810814 1102 6 1.295560615566977 1102 7 0.7412971287473411 1102 8 0.5803428746984309 1102 9 0.6356876029307159 1102 10-11 0.49933842271696705 1102 12-13 0.5842010603455634 1102 14-15 0.6522042889943904 1102 16-17 0.5516880101761359 1102 18-19 0.5232626889866481 1102 20-21 0.8105342346848019 1102 22-23 0.6087786049626516 1102 24-25 0.5644354337023856 1102 26-27 0.5261942477164823 1102 28-29 0.5966858029100379 1102 30-31 0.633515924005863 1102 32-33 0.735781942889723 1102 34-35 0.7792501416131614 1102 36-37 0.7126992034145232 1102 38-39 0.6185406172522008 1102 40-41 0.6509172589628278 1102 42-43 0.551548827637319 1102 44-45 0.4763722924674454 1102 46-47 0.5375902513113076 1102 48-49 0.5765066910625833 1102 50-51 0.6502094217572676 1102 52-53 0.5683021580124361 1102 54-55 0.6250907729712623 1102 56-57 0.7871950175990818 1102 58-59 0.6297568582119126 1102 60-61 0.6144115940005932 1102 62-63 0.6831593134841683 1102 64-65 0.7062028375317695 1102 66-67 0.886967147849802 1102 68-69 0.6883994270359963 1102 70-71 0.8098576697273074 1102 72-73 0.7400386380948092 1102 74-75 0.6997719909332147 1102 76-77 0.638592663268831 1102 78-79 0.6518970380438631 1102 80-81 0.619184372421671 1102 82-83 0.572787584639201 1102 84-85 0.4537127474722489 1102 86-87 0.3655345858133323 1102 88-89 0.2812821789872899 1102 90-91 0.3017642903540043 1102 92-93 0.403159486398593 1102 94-95 0.3853671485451997 1102 96-97 0.4459156696371771 1102 98-99 0.3992151104662902 1102 100-101 0.2504439086926453 1103 1 1.2972028310567723 1103 2 1.2664146457751038 1103 3 1.8802254798638316 1103 4 2.846230688734721 1103 5 2.4466639929080785 1103 6 1.2748916746846106 1103 7 0.6760582949849478 1103 8 0.5992976183905512 1103 9 0.5562290026011851 1103 10-11 0.408771696490696 1103 12-13 0.5008762184421585 1103 14-15 0.5047926168963599 1103 16-17 0.5436628689638994 1103 18-19 0.5464803738720576 1103 20-21 0.8003124253687588 1103 22-23 0.5952965591331534 1103 24-25 0.4485314724036158 1103 26-27 0.36190999350304054 1103 28-29 0.3974354219734906 1103 30-31 0.4732220571331567 1103 32-33 0.6115589892463618 1103 34-35 0.6038507352345945 1103 36-37 0.6138418401997043 1103 38-39 0.5289832113799307 1103 40-41 0.4869424829991331 1103 42-43 0.4739757658788122 1103 44-45 0.44098285856609465 1103 46-47 0.4801328700994105 1103 48-49 0.5001911592183248 1103 50-51 0.3982630130948408 1103 52-53 0.2659000906562454 1103 54-55 0.42593799331318394 1103 56-57 0.5571025346982168 1103 58-59 0.4216588971445887 1103 60-61 0.4064583507603885 1103 62-63 0.4571828579338799 1103 64-65 0.35776622468743824 1103 66-67 0.622729632852753 1103 68-69 0.5088154235289437 1103 70-71 0.6237559263075809 1103 72-73 0.5469132981549762 1103 74-75 0.5228095154777179 1103 76-77 0.43164130660995426 1103 78-79 0.4475405200860152 1103 80-81 0.4135028469631621 1103 82-83 0.40562215252153777 1103 84-85 0.4253109570154088 1103 86-87 0.29596312689537285 1103 88-89 0.13597323925393567 1103 90-91 0.2422515561499985 1103 92-93 0.36085571256925064 1103 94-95 0.33819943465791624 1103 96-97 0.29420152497536023 1103 98-99 0.2528397763300312 1103 100-101 0.08863090868604928 1104 1 1.22090113516278 1104 2 1.1907800356690466 1104 3 1.7889638898477926 1104 4 2.752092765889131 1104 5 2.3683967068993397 1104 6 1.1852420738440301 1104 7 0.6569332657766296 1104 8 0.48002662464487855 1104 9 0.4887571509050943 1104 10-11 0.3245424415300775 1104 12-13 0.39954880800792836 1104 14-15 0.44105048293011606 1104 16-17 0.435388759541695 1104 18-19 0.32208351180096884 1104 20-21 0.7253122245271015 1104 22-23 0.45828118585453836 1104 24-25 0.3840585970292878 1104 26-27 0.39018049095556506 1104 28-29 0.4920045490387679 1104 30-31 0.4809614281942558 1104 32-33 0.5413192533264208 1104 34-35 0.5419781704050237 1104 36-37 0.5029432289088902 1104 38-39 0.4325615713282147 1104 40-41 0.4229621657361591 1104 42-43 0.3994082238549552 1104 44-45 0.3170336362750774 1104 46-47 0.40911728272759973 1104 48-49 0.35216081504871966 1104 50-51 0.30679110704662094 1104 52-53 0.3920836381083532 1104 54-55 0.36916806213037034 1104 56-57 0.40852287072501525 1104 58-59 0.302235731978179 1104 60-61 0.23917716119707677 1104 62-63 0.3021719511093153 1104 64-65 0.18962651511163386 1104 66-67 0.48196779149475333 1104 68-69 0.3611559161298672 1104 70-71 0.4625526149768646 1104 72-73 0.36321598980708814 1104 74-75 0.23669487060520567 1104 76-77 0.18311668862614994 1104 78-79 0.2648077614789983 1104 80-81 0.27573135108273306 1104 82-83 0.3144319644609226 1104 84-85 0.2438551804172988 1104 86-87 0.20475764475439462 1104 88-89 0.16864244969759312 1104 90-91 0.17601623211806938 1104 92-93 0.1768570951127444 1104 94-95 0.17204095931656127 1104 96-97 0.13876057038014622 1104 98-99 0.13682561300617024 1104 100-101 -0.07771451756201486 1105 1 1.1826626797376854 1105 2 1.1350010519963298 1105 3 1.7560052441202814 1105 4 2.69205541361562 1105 5 2.328547242466172 1105 6 1.1769357100098787 1105 7 0.6684317413163896 1105 8 0.4579311104040329 1105 9 0.3750234443117222 1105 10-11 0.2489311934132914 1105 12-13 0.30548797522712334 1105 14-15 0.29818453394145905 1105 16-17 0.27871203503807607 1105 18-19 0.27639714396627824 1105 20-21 0.6297126272528217 1105 22-23 0.4037067339400622 1105 24-25 0.2825970690893058 1105 26-27 0.26111197900248584 1105 28-29 0.4018552706585865 1105 30-31 0.40280851913680493 1105 32-33 0.4057981869782452 1105 34-35 0.4648175619665409 1105 36-37 0.47757998189882755 1105 38-39 0.2853134427018631 1105 40-41 0.32543419759291226 1105 42-43 0.20498035510838264 1105 44-45 0.2876278100597105 1105 46-47 0.3687619220173701 1105 48-49 0.2823132741241636 1105 50-51 0.3227288468628302 1105 52-53 0.29841974892659096 1105 54-55 0.304576235492803 1105 56-57 0.3182461179956064 1105 58-59 0.23237947631475464 1105 60-61 0.22205725181946434 1105 62-63 0.3026560385359076 1105 64-65 0.3106276774021879 1105 66-67 0.4707840291132186 1105 68-69 0.32634164889385886 1105 70-71 0.3798703215649155 1105 72-73 0.3690462623792641 1105 74-75 0.281825911103013 1105 76-77 0.20919187746096668 1105 78-79 0.27001475474637715 1105 80-81 0.2682754693323446 1105 82-83 0.2066772303618194 1105 84-85 0.18895431562175702 1105 86-87 0.14518719191461216 1105 88-89 0.10687730579367738 1105 90-91 0.042640407968097094 1105 92-93 0.15875318005457473 1105 94-95 0.1015898344360906 1105 96-97 0.09898136770776489 1105 98-99 0.07806531215242885 1105 100-101 0.039629224947546504 1106 1 1.1061571218435908 1106 2 1.0669033402511232 1106 3 1.6547412059701472 1106 4 2.668817282228936 1106 5 2.2520437725888485 1106 6 1.1156273643207086 1106 7 0.5952551348379842 1106 8 0.3914281969951219 1106 9 0.31287905592352416 1106 10-11 0.22342315065289853 1106 12-13 0.3221307788200889 1106 14-15 0.28614741907584573 1106 16-17 0.27321640109389733 1106 18-19 0.2747499967681719 1106 20-21 0.5403080169819816 1106 22-23 0.32918792514525563 1106 24-25 0.22703105935667622 1106 26-27 0.17043035908421444 1106 28-29 0.31383525608925567 1106 30-31 0.31960064231112995 1106 32-33 0.3451164052362117 1106 34-35 0.3041275704136126 1106 36-37 0.33674376095080305 1106 38-39 0.1692453332695294 1106 40-41 0.2246453127149266 1106 42-43 0.1586395726761154 1106 44-45 0.20506488688393887 1106 46-47 0.21887790249457595 1106 48-49 0.21410966598510583 1106 50-51 0.16729269894189258 1106 52-53 0.18151665978857068 1106 54-55 0.17042343118004055 1106 56-57 0.32092223504471207 1106 58-59 0.19126876214249222 1106 60-61 0.1521139332489696 1106 62-63 0.19474764838712844 1106 64-65 0.18220211012178567 1106 66-67 0.40325609946837915 1106 68-69 0.2736111726407984 1106 70-71 0.39691794231357846 1106 72-73 0.23765503830633605 1106 74-75 0.23697765166436113 1106 76-77 0.15507840858871447 1106 78-79 0.17225514832557565 1106 80-81 0.16553207915874424 1106 82-83 0.17564573416437668 1106 84-85 0.09918434185957992 1106 86-87 0.11802016500575618 1106 88-89 0.07951058801840105 1106 90-91 0.11964864927044161 1106 92-93 0.1634911604703433 1106 94-95 0.04820095648092959 1106 96-97 0.009249937150844545 1106 98-99 0.011714602531011309 1106 100-101 -0.011266738235402585 1107 1 0.7818176176917611 1107 2 0.8867710166247775 1107 3 1.4747726948009898 1107 4 0.981129962804772 1107 5 1.7160911413345232 1107 6 0.83644259559658 1107 7 0.4097686832055558 1107 8 0.31726331967784205 1107 9 0.18696169051028733 1107 10-11 0.04701542102758083 1107 12-13 0.13629255390547002 1107 14-15 0.14242546121818833 1107 16-17 0.12427391414507127 1107 18-19 0.10162413279195448 1107 20-21 0.442041056015654 1107 22-23 0.2365316530050805 1107 24-25 0.09024660633745185 1107 26-27 0.12918390774078148 1107 28-29 0.1822394930883462 1107 30-31 0.14894186017266975 1107 32-33 0.2740475080010327 1107 34-35 0.15062258022114605 1107 36-37 0.19795078257151744 1107 38-39 0.22102672390148115 1107 40-41 0.06679288755590562 1107 42-43 -0.024162430632657106 1107 44-45 0.031129421909746213 1107 46-47 0.11069932923646064 1107 48-49 0.07195043966329706 1107 50-51 0.054048846077058954 1107 52-53 0.0403643943077725 1107 54-55 0.018106300997118296 1107 56-57 -0.02348485310081827 1107 58-59 -0.08963842228052243 1107 60-61 -0.184762961097519 1107 62-63 -0.004688105693013256 1107 64-65 -0.0419096282183844 1107 66-67 0.15309267805018578 1107 68-69 0.06698098294538113 1107 70-71 0.176843910978846 1107 72-73 0.09868050883691382 1107 74-75 -0.0093504907955122 1107 76-77 -0.11577105317027048 1107 78-79 -0.05564957408363824 1107 80-81 -0.08053472723062072 1107 82-83 0.050185544870927856 1107 84-85 0.051993653641730475 1107 86-87 0.07796758764660439 1107 88-89 0.12776909923748292 1107 90-91 0.07664291540628554 1107 92-93 0.11804572628968657 1107 94-95 0.07644993297418878 1107 96-97 -0.015394697323468876 1107 98-99 0.004079804496296191 1107 100-101 -0.0852569118778348 1108 1 -0.1061513783031387 1108 2 0.40616243851636824 1108 3 1.1196985244985527 1108 4 -2.8900997455850614 1108 5 0.1247741056344438 1108 6 0.10695195510935918 1108 7 0.031937469286361875 1108 8 0.09981623377149873 1108 9 0.00795003632386937 1108 10-11 -0.07595963198360778 1108 12-13 0.05809789921023878 1108 14-15 0.059158781501473356 1108 16-17 0.0017484798460500883 1108 18-19 0.03095231241969998 1108 20-21 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-0.012060165362903774 2312 28-29 -0.031346005581937675 2312 30-31 0.004852732048746589 2312 32-33 0.05017544776762861 2312 34-35 0.04917018886604296 2312 36-37 0.01501125633712519 2312 38-39 -0.05695365527414964 2312 40-41 -0.01158217690005614 2312 42-43 -0.03321955765657236 2312 44-45 -0.09346670691908088 2312 46-47 -0.08256996262475269 2312 48-49 -0.07160536455259603 2312 50-51 -0.087900255741161 2312 52-53 -0.19628575062527887 2312 54-55 -0.2422598542708414 2312 56-57 -0.22264082000739194 2312 58-59 -0.13489261320718526 2312 60-61 -0.16327549406126707 2312 62-63 -0.1261951619466828 2312 64-65 -0.2245923848146134 2312 66-67 0.027919192276684157 2312 68-69 -0.11748497820473602 2312 70-71 -0.5890633625300836 2312 72-73 -0.26388294473629514 2312 74-75 -0.22037924101564954 2312 76-77 -0.14016211481680685 2312 78-79 -0.2030984207277129 2312 80-81 -0.14497173773084882 2312 82-83 -0.13243494459595695 2312 84-85 -0.14269756718464421 2312 86-87 -0.14974701154166326 2312 88-89 -0.14972544927177012 2312 90-91 -0.2654437935550362 2312 92-93 -0.176045501236608 2312 94-95 -0.2684513624663758 2312 96-97 -0.2390996007457531 2312 98-99 -0.26985279536631523 2312 100-101 -0.34689263069437715 2313 1 -3.260693837471532 2313 2 -0.9871403497385778 2313 3 -5.464578436485844 2313 4 -6.295865081204685 2313 5 -5.877222826750117 2313 6 -2.864330108628078 2313 7 -1.3891213172900834 2313 8 -0.8302652314581351 2313 9 -0.5581688312055633 2313 10-11 -0.3945822619618795 2313 12-13 -0.32985472295509055 2313 14-15 -0.490758311350433 2313 16-17 -0.43574558688818144 2313 18-19 -0.5272619137637378 2313 20-21 -0.09726530890873875 2313 22-23 -0.21260082003414027 2313 24-25 -0.3824777845368601 2313 26-27 -0.45092608490297437 2313 28-29 -0.4245143687685413 2313 30-31 -0.35171567185758335 2313 32-33 -0.4027628989952632 2313 34-35 -0.3527542691965948 2313 36-37 -0.35720990542224484 2313 38-39 -0.3636844020440364 2313 40-41 -0.3468091485599132 2313 42-43 -0.4719702865381308 2313 44-45 -1.203584516559438 2313 46-47 -0.5191552148168412 2313 48-49 -0.303094838389363 2313 50-51 -0.4926026018516865 2313 52-53 -0.6473179276563457 2313 54-55 -1.1621865528399695 2313 56-57 -0.6644729240729461 2313 58-59 -0.4181807522452772 2313 60-61 -0.4506460457389494 2313 62-63 -0.5387307768411986 2313 64-65 -0.5750088412141494 2313 66-67 -0.6847233829899153 2313 68-69 -0.8074083675007486 2313 70-71 -0.37601557859661483 2313 72-73 -0.4130854291135151 2313 74-75 -0.9236740613537364 2313 76-77 -0.8169122408673744 2313 78-79 -0.68427786096116 2313 80-81 -0.606007599241039 2313 82-83 -0.5540389989974948 2313 84-85 -0.6839933346655762 2313 86-87 -0.7161239455174524 2313 88-89 -0.6076460360641285 2313 90-91 -0.5823645986251371 2313 92-93 -0.6587598005329127 2313 94-95 -0.757404035998988 2313 96-97 -0.7131925986928955 2313 98-99 -0.7191454015939946 2313 100-101 -0.8813943109261295 2314 1 -4.450897421872849 2314 2 -6.899482961661597 2314 3 -7.071716605135624 2314 4 -6.7456643032572146 2314 5 -6.38026019739619 2314 6 -3.344061808582712 2314 7 -1.7714023235405065 2314 8 -1.0570665985905379 2314 9 -0.7728073925297281 2314 10-11 -0.6795716208252571 2314 12-13 -0.4949642994457264 2314 14-15 -0.5543036588151438 2314 16-17 -0.5366546829261054 2314 18-19 -0.613475557532027 2314 20-21 -0.26695415006520307 2314 22-23 -0.5256347932316103 2314 24-25 -0.5688944112318666 2314 26-27 -1.14290443044775 2314 28-29 -0.6935630294119619 2314 30-31 -0.6850783814578847 2314 32-33 -0.4688613107064086 2314 34-35 -0.6340224515852952 2314 36-37 -0.5641015633128674 2314 38-39 -0.5112636802583737 2314 40-41 -0.5007876628347674 2314 42-43 -0.5621597542802519 2314 44-45 -0.637436059799775 2314 46-47 -0.5432350364344032 2314 48-49 -0.5750850834962762 2314 50-51 -0.7567228403336586 2314 52-53 -0.6750084146576967 2314 54-55 -0.7179249428394172 2314 56-57 -0.6276999353015285 2314 58-59 -0.736988796227557 2314 60-61 -0.7451095272048534 2314 62-63 -0.7411319821499234 2314 64-65 -0.672423950529442 2314 66-67 -0.5237372836155885 2314 68-69 -0.7045454530594597 2314 70-71 -0.5982441631079638 2314 72-73 -0.6519152529199417 2314 74-75 -0.648888524985864 2314 76-77 -0.8306595430477088 2314 78-79 -0.7390758917025764 2314 80-81 -0.7161425232182523 2314 82-83 -0.7028998291329316 2314 84-85 -0.7891113394020302 2314 86-87 -0.7697098902089081 2314 88-89 -0.7495403565348511 2314 90-91 -0.7819830619688126 2314 92-93 -0.7547258783123922 2314 94-95 -0.7801738674186538 2314 96-97 -0.7934257306166046 2314 98-99 -0.9051399645206253 2314 100-101 -1.0101601771722102 2315 1 -3.258538511704856 2315 2 -4.506107066667312 2315 3 -4.18022182895055 2315 4 -4.748153482617592 2315 5 -5.7131450258420635 2315 6 -3.018584765906869 2315 7 -1.4367308962230396 2315 8 -1.2475594047536873 2315 9 -3.4061924006102657 2315 10-11 -1.359785886891963 2315 12-13 -4.852288266385685 2315 14-15 -1.992350227698232 2315 16-17 -2.319698552907269 2315 18-19 -1.9868563538067292 2315 20-21 -1.5960179591669572 2315 22-23 -1.7821753820256063 2315 24-25 -1.9241807320506155 2315 26-27 -1.4432343420852405 2315 28-29 -1.096690884222454 2315 30-31 -1.64435911924393 2315 32-33 -1.9547295058604988 2315 34-35 -1.1407870868536705 2315 36-37 -1.5556304466734971 2315 38-39 -1.3017576346464566 2315 40-41 -1.3138652821396164 2315 42-43 -1.0494730019777236 2315 44-45 -0.5684305989582086 2315 46-47 -4.447609192277451 2315 48-49 -2.431822890810963 2315 50-51 -1.5526689990644655 2315 52-53 -2.510095828035979 2315 54-55 -2.1000133047145226 2315 56-57 -2.6596959845077635 2315 58-59 -1.6819232122086518 2315 60-61 -2.7668353842163214 2315 62-63 -3.0390040617578293 2315 64-65 -2.43556457593116 2315 66-67 -3.764072767786402 2315 68-69 -2.441471021871095 2315 70-71 -1.7697636054887695 2315 72-73 -1.696059804960882 2315 74-75 -1.178635538714289 2315 76-77 -1.649824888140337 2315 78-79 -0.8824934038281391 2315 80-81 -0.9928287514422394 2315 82-83 -0.9027638336649559 2315 84-85 -1.00009695898423 2315 86-87 -1.1325966393298224 2315 88-89 -1.4164779377006909 2315 90-91 -1.0761324900631344 2315 92-93 -1.2926787842349121 2315 94-95 -0.8251363054456924 2315 96-97 -1.288989348565785 2315 98-99 -1.1572233327061419 2315 100-101 -1.4389227339106334 2316 1 -0.4209673617339895 2316 2 -1.9041482011031476 2316 3 -1.9458295635675462 2316 4 0.21928405690530184 2316 5 1.270613867979641 2316 6 -1.0926015193650542 2316 7 -1.2380436303338627 2316 8 -1.1237656151823643 2316 9 -0.690088710539392 2316 10-11 -1.742725068318741 2316 12-13 -1.7317738334340333 2316 14-15 -2.1203409115253677 2316 16-17 -3.6204803110821615 2316 18-19 -1.8153243310469236 2316 20-21 -1.8841718021565654 2316 22-23 -1.3270652550416884 2316 24-25 -1.4942033116005646 2316 26-27 -1.030647196990337 2316 28-29 -1.2860655028103665 2316 30-31 -1.5336115797523888 2316 32-33 -0.9193778307435423 2316 34-35 -1.990739258206652 2316 36-37 -1.9155818272672747 2316 38-39 -2.441272353594961 2316 40-41 -2.424705737033463 2316 42-43 -2.724801952716021 2316 44-45 -2.92889974793804 2316 46-47 -2.4383653221762387 2316 48-49 -2.459461721962491 2316 50-51 -2.117276959668459 2316 52-53 -1.811354451643929 2316 54-55 -1.7646168982385575 2316 56-57 -3.77162459016634 2316 58-59 -3.7483677610348494 2316 60-61 -3.720135070559131 2316 62-63 -4.468923733052915 2316 64-65 -3.0819591131880983 2316 66-67 -2.0343092462269325 2316 68-69 -2.510628034138634 2316 70-71 -1.4377779204881165 2316 72-73 -1.4668046218798239 2316 74-75 -1.9677583810596033 2316 76-77 -1.8041832088249237 2316 78-79 -1.1062412951709675 2316 80-81 -1.284776762285496 2316 82-83 -1.0553157274844338 2316 84-85 -1.0041924210803224 2316 86-87 -1.0039808436417879 2316 88-89 -0.916351457675713 2316 90-91 -0.9861655098743789 2316 92-93 -1.3259358187602004 2316 94-95 -1.8440247469789774 2316 96-97 -1.196848129281804 2316 98-99 -1.1760085102993543 2316 100-101 -1.0415264676124494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12107.0 3 1826.0 4 2080.0 5 3664.0 6 8420.0 7 19781.0 8 41301.0 9 66045.0 10 87064.0 11 102196.0 12 114875.0 13 130855.0 14 150829.0 15 172871.0 16 196842.0 17 222222.0 18 250293.0 19 285336.0 20 315851.0 21 297081.0 22 333036.0 23 384625.0 24 462431.0 25 575178.0 26 737236.0 27 937835.0 28 1180205.0 29 1475006.0 30 1819835.0 31 2227798.0 32 2723495.0 33 3365680.0 34 4263421.0 35 5598183.0 36 7932040.0 37 1.2303857E7 38 1.4009716E7 39 3475379.0 40 5648.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.02240677456639 16.13987224643485 14.571172785975786 39.26654819302297 2 20.01535085731153 19.20602923460083 45.67627349295194 15.1023464151357 3 22.688752449009023 23.148104178897885 33.563447661652695 20.599695710440397 4 22.8457061261828 31.683128976289225 28.6418950190781 16.82926987844987 5 22.753736263631826 33.95018675189817 28.526082609571706 14.7699943748983 6 17.701479344241445 36.03289608543806 30.10278307038588 16.162841499934615 7 15.604110128103718 18.272390138300402 48.118545511494474 18.004954222101404 8 17.10654006161786 23.622180987571937 38.73626622690414 20.53501272390606 9 19.80272986498566 21.77193607996652 37.47848972087085 20.94684433417698 10-11 21.394859719650334 33.59827951255098 27.22906016177513 17.777800606023554 12-13 19.612843862959746 28.1366745075657 33.79922564880728 18.451255980667273 14-15 19.901109547778535 27.796168846133096 33.825421815070904 18.477299791017465 16-17 20.62547517886094 28.10594914694491 31.703346051130072 19.565229623064077 18-19 21.205272395553724 28.431195081444265 29.813552716194437 20.54997980680757 20-21 20.809273123905047 29.022938422921946 29.798315058824652 20.36947339434835 22-23 20.712241117322154 28.987578512886515 31.67130982626403 18.628870543527306 24-25 20.48976058402797 28.882973427125464 31.53315013622553 19.09411585262104 26-27 20.980684777536624 28.822393113416112 30.427212677379988 19.76970943166728 28-29 21.23928947257263 28.87856365809754 29.412583946596943 20.469562922732887 30-31 20.517297372373882 28.255285402031426 30.790020491145288 20.437396734449404 32-33 21.406158416243215 28.58758816261383 30.510701041911524 19.49555237923143 34-35 19.606284314869647 30.460581093478574 29.50523897848576 20.427895613166015 36-37 19.987984604403067 28.95585335655974 30.43775918786572 20.618402851171478 38-39 20.734539961448206 27.81684145850642 31.27476472126255 20.173853858782824 40-41 21.289454892500597 28.422584399148228 30.19102546450417 20.096935243847007 42-43 20.97237597937938 29.452420284979198 30.149363008170855 19.425840727470572 44-45 20.637640692812337 29.092225983799867 30.441558991865936 19.828574331521857 46-47 20.57377622806331 28.647989987427696 30.33396304893013 20.44427073557887 48-49 21.102040058419984 28.74585383370643 29.498358932688635 20.653747175184947 50-51 20.693066052939 28.911104079696354 30.016355594560768 20.379474272803883 52-53 20.454559401991308 28.902350079274292 30.73538050996987 19.907710008764536 54-55 20.19460829867011 29.12010407518453 30.652322910073742 20.032964716071625 56-57 20.937127974705838 28.24057872771678 29.939725214877942 20.882568082699436 58-59 20.87795037330124 28.430329877473365 29.4965501021336 21.195169647091788 60-61 21.41231832501472 28.404128333830457 29.872246971449922 20.3113063697049 62-63 19.72269896506399 30.45263901839433 29.720480046904147 20.104181969637533 64-65 19.57124720862609 30.183596241191303 29.845684975665048 20.39947157451756 66-67 20.289536775862086 28.70631513090679 30.464518593811658 20.539629499419465 68-69 21.2653426154319 29.38836960455146 29.095399949932837 20.2508878300838 70-71 20.435621950802656 30.4432526902723 28.725133995946962 20.395991362978084 72-73 19.912744864390024 30.271632682944787 29.448443418023356 20.367179034641833 74-75 20.259554990006542 29.07444174272671 30.145815991812498 20.52018727545426 76-77 20.6690866961169 29.22706381353984 29.163299633665602 20.940549856677663 78-79 20.46444839723994 30.1995399517774 28.433145468364433 20.902866182618222 80-81 20.564796549419878 29.920575048318977 29.821986504998314 19.692641897262835 82-83 20.3498214872725 30.05909564286522 29.503118536847374 20.08796433301491 84-85 20.22342502286506 30.12230120933313 29.078524356667696 20.575749411134115 86-87 20.89576402644087 30.42284523504596 28.097026188517976 20.584364549995193 88-89 20.413449628734504 30.091872992733208 29.05678912136567 20.437888257166613 90-91 20.562120376391224 30.44764001650574 28.656472085758804 20.33376752134423 92-93 19.877438270658164 31.00023859935884 28.679999783324682 20.442323346658313 94-95 19.81979710407943 30.96888660173057 28.64025694437073 20.57105934981927 96-97 20.381775885694278 29.66175352074417 29.414115271548525 20.542355322013027 98-99 20.78244103943954 30.289243341612305 28.234597438234804 20.693718180713354 100-101 21.171903909543627 31.058156659974756 27.36959613664938 20.400343293832236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4320.0 1 4275.0 2 4573.0 3 6188.5 4 8713.0 5 11112.5 6 12827.0 7 13940.5 8 16542.0 9 18886.5 10 25231.5 11 39024.0 12 46373.5 13 62517.5 14 71213.5 15 81148.5 16 167030.5 17 210971.0 18 228604.5 19 261234.5 20 280801.0 21 265858.5 22 222493.5 23 253562.5 24 339685.5 25 423094.0 26 485391.5 27 555798.5 28 658270.0 29 797268.0 30 977270.0 31 1214770.0 32 1547615.0 33 1988087.5 34 2502652.0 35 3080967.5 36 3620526.0 37 3817774.0 38 3538971.0 39 3073295.0 40 2713615.5 41 2500599.5 42 2369889.5 43 2283197.5 44 2195975.5 45 2132673.5 46 2087895.0 47 2034154.5 48 1975153.5 49 1891447.5 50 1779104.0 51 1667559.5 52 1544751.0 53 1387053.5 54 1227424.0 55 1088682.0 56 955157.5 57 805313.0 58 660565.0 59 522955.0 60 394888.5 61 295846.0 62 221411.5 63 162544.5 64 120206.5 65 88524.5 66 64686.0 67 48869.5 68 36638.0 69 26900.5 70 19491.0 71 13074.0 72 9675.0 73 7571.5 74 5653.0 75 4026.0 76 2829.5 77 2142.0 78 1702.0 79 1315.0 80 1011.5 81 776.0 82 595.0 83 481.5 84 388.0 85 302.5 86 234.5 87 156.5 88 92.5 89 66.0 90 48.5 91 40.5 92 31.0 93 16.5 94 8.0 95 4.0 96 2.5 97 1.0 98 1.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21227402469098036 2 1.2067795123171686E-4 3 3.4694910979118594E-5 4 0.0018433557050644748 5 0.0026941352612480786 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.042759215070178075 22-23 0.0 24-25 0.018352853670758538 26-27 0.08551616742877055 28-29 0.06030654341646491 30-31 0.05351388323650964 32-33 0.07904632076836014 34-35 0.020331217833763498 36-37 0.020881056749063008 38-39 0.019998599230681077 40-41 0.02793392876136166 42-43 0.027004708536877437 44-45 0.03708961407387298 46-47 0.038398969844737105 48-49 0.037337003873898 50-51 0.17038670781845142 52-53 0.32610274795310207 54-55 0.28882382034323434 56-57 0.397915179782316 58-59 0.3770740976045985 60-61 0.3625806756616693 62-63 0.35815858298622205 64-65 0.370128327274018 66-67 0.3289220865887531 68-69 0.2578895360193741 70-71 0.28724897307966046 72-73 0.25880140878836877 74-75 0.2747603136015681 76-77 0.24991649462893362 78-79 0.23209236123200905 80-81 0.20037517869953306 82-83 0.18562003644987007 84-85 0.14423805849812396 86-87 0.07963160883183397 88-89 0.024014158057916457 90-91 0.01651553186325565 92-93 0.026950403458823168 94-95 0.031191479207422813 96-97 0.04184960501276901 98-99 0.05659041675572322 100-101 0.05661304387157917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.6292143E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.92025439924592 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25573420881243 40.06386668702179 2 9.98764048947262 9.971711081653643 3 3.5737513223242754 5.352077255102079 4 1.7827562175411347 3.5598257564596336 5 1.0478532534115947 2.6154550491692152 6 0.6732455525367887 2.0165153553478428 7 0.4617274261237285 1.6134685402643887 8 0.3476949060096446 1.3885614529058687 9 0.2603414972068334 1.1696682394121123 >10 1.4331860899375384 12.98768393925035 >50 0.10687966542995966 3.6836737473350554 >100 0.0605070332408723 5.703756041725081 >500 0.004914542835505001 1.6718557447141433 >1k 0.003122681621443032 3.035859396356775 >5k 3.4082071760703573E-4 1.190974561609401 >10k+ 3.0429277812706127E-4 3.9750471516727925 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT 139060 0.20976844872853181 No Hit CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 135435 0.20430022906334466 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 107826 0.16265275961888878 No Hit CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT 101856 0.1536471675082219 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 89274 0.13466754272825363 No Hit GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT 82699 0.1247493236113969 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 68919 0.10396254651173367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0019142540014131086 4.525423171189382E-6 0.0 1.0559320732775225E-5 0.0 2 0.0019338641684882626 6.033897561585843E-6 0.0 5.1288129273479665E-5 1.5084743903964607E-6 3 0.002016830259960068 9.050846342378765E-6 0.0 5.883050122546197E-5 1.5084743903964607E-6 4 0.0023200336124297566 9.050846342378765E-6 0.0 7.542371951982304E-5 1.5084743903964607E-6 5 0.0024180844478055266 9.050846342378765E-6 0.0 7.542371951982304E-5 1.5084743903964607E-6 6 0.0026081522209954805 9.050846342378765E-6 0.0 8.749151464299472E-5 1.5084743903964607E-6 7 0.0026926267868576824 9.050846342378765E-6 0.0 9.20169378141841E-5 1.5084743903964607E-6 8 0.0028751521880956542 1.0559320732775225E-5 0.0 9.955930976616641E-5 1.5084743903964607E-6 9 0.003105948769826313 1.0559320732775225E-5 0.0 1.071016817181487E-4 1.5084743903964607E-6 10-11 0.0037281944558648526 1.2067795123171685E-5 0.0 1.2143218842691509E-4 1.5084743903964607E-6 12-13 0.004686075693766605 1.3576269513568147E-5 0.0 1.3349998355008678E-4 1.5084743903964607E-6 14-15 0.005883804359741395 1.3576269513568147E-5 0.0 1.463220158684567E-4 1.5084743903964607E-6 16-17 0.007319117742203627 1.4330506708766376E-5 0.0 1.493389646492496E-4 1.5084743903964607E-6 18-19 0.009009363296642862 1.6593218294361068E-5 0.0 1.5989828538202482E-4 1.5084743903964607E-6 20-21 0.010874591880368085 1.885592987995576E-5 0.0 1.7272031770039476E-4 3.0169487807929213E-6 22-23 0.01277753232385322 1.9610167075153988E-5 0.0 1.8554235001876467E-4 3.0169487807929213E-6 24-25 0.014662371074653598 1.9610167075153988E-5 0.0 2.1420336343629744E-4 3.0169487807929213E-6 26-27 0.016564557280943536 1.9610167075153988E-5 0.0 2.3532200490184786E-4 3.0169487807929213E-6 28-29 0.018575353643342018 2.111864146555045E-5 0.0 2.511609860010107E-4 3.0169487807929213E-6 30-31 0.020502429677073497 2.111864146555045E-5 0.0 2.6247454392898416E-4 3.0169487807929213E-6 32-33 0.02257884467545422 2.1872878660748683E-5 0.0 2.7454233905215583E-4 3.0169487807929213E-6 34-35 0.024568522396387155 2.338135305114514E-5 0.0 3.001864036888957E-4 3.0169487807929213E-6 36-37 0.026712818742335727 2.6398301831938065E-5 0.0 3.1527114759286024E-4 3.0169487807929213E-6 38-39 0.02889331847365381 3.0169487807929215E-5 0.0 3.2583046832563553E-4 3.0169487807929213E-6 40-41 0.031180919886690042 3.167796219832568E-5 0.0 3.409152122296001E-4 3.0169487807929213E-6 42-43 0.03370761449060411 3.394067378392037E-5 0.0 3.552457189383665E-4 3.0169487807929213E-6 44-45 0.036336131115869946 3.9974571345506205E-5 0.0 3.6957622564713286E-4 3.0169487807929213E-6 46-47 0.03901744434479965 4.22372829311009E-5 0.0 3.8692368113669214E-4 3.0169487807929213E-6 48-49 0.04194463889936399 4.299152012629913E-5 0.0 3.9522029028387273E-4 3.0169487807929213E-6 50-51 0.04518408765274039 4.4499994516695594E-5 0.0 4.2161859211581077E-4 3.0169487807929213E-6 52-53 0.048692044847607356 4.600846890709205E-5 0.0 4.306694384581895E-4 3.0169487807929213E-6 54-55 0.05230408677541168 4.676270610229028E-5 0.0 4.480168939477488E-4 3.0169487807929213E-6 56-57 0.05645767100936833 4.9779654883083206E-5 0.0 4.7290672138929047E-4 3.771185975991152E-6 58-59 0.06115355178667252 5.1288129273479665E-5 0.0 4.940253628548409E-4 4.525423171189382E-6 60-61 0.06643472062745054 5.1288129273479665E-5 0.0 5.128812927347967E-4 4.525423171189382E-6 62-63 0.07275145713723571 5.2042366468677894E-5 0.0 5.324914598099507E-4 4.525423171189382E-6 64-65 0.0800358799684602 5.355084085907436E-5 0.0 5.475762037139152E-4 7.542371951982304E-6 66-67 0.08895171785289849 5.883050122546197E-5 0.0 5.558728128610957E-4 7.542371951982304E-6 68-69 0.10029695374307028 6.109321281105667E-5 0.0 5.686948451794657E-4 7.542371951982304E-6 70-71 0.11550086712387621 6.335592439665135E-5 0.0 5.837795890834302E-4 7.542371951982304E-6 72-73 0.13493378845815862 6.486439878704781E-5 0.0 5.92830435425809E-4 7.542371951982304E-6 74-75 0.16059972597355918 6.561863598224604E-5 0.0 5.98864332987395E-4 8.296609147180534E-6 76-77 0.19382085747326044 6.637287317744427E-5 0.0 6.064067049393773E-4 9.050846342378765E-6 78-79 0.237483648703286 6.788134756784073E-5 0.0 6.162117884769542E-4 9.050846342378765E-6 80-81 0.2959702177677376 7.014405915343542E-5 0.0 6.373304299425046E-4 9.050846342378765E-6 82-83 0.3718623185857787 7.089829634863366E-5 0.0 6.463812762848833E-4 9.050846342378765E-6 84-85 0.47375825518266923 7.089829634863366E-5 0.0 6.576948342128569E-4 9.805083537576994E-6 86-87 0.6090269249554958 7.089829634863366E-5 0.0 6.637287317744427E-4 1.0559320732775225E-5 88-89 0.7779225058390404 7.089829634863366E-5 0.0 6.735338153120198E-4 1.0559320732775225E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCAG 33080 0.0 33.10522 1 CGACGGC 16280 0.0 27.023876 1 CACGGTC 24285 0.0 24.122152 1 ACGGCCA 23395 0.0 22.2509 3 GACGGCC 23745 0.0 21.882992 2 CTGGAAT 192195 0.0 20.730217 1 CGGCGAG 5670 0.0 20.696884 1 ACGGTCT 31810 0.0 18.242569 2 CGGACGT 3205 0.0 17.195745 1 CTTTTAT 127185 0.0 16.824942 1 TCACGGT 21900 0.0 16.73415 2 GGCCAGT 65470 0.0 16.539486 2 CGGTCTT 37005 0.0 15.989043 3 CGACGGA 2600 0.0 15.715065 1 TATGGCG 266185 0.0 15.523237 7 TATTATA 104025 0.0 15.458479 9 TGGCGAG 265050 0.0 15.333936 9 ATGGCGA 272265 0.0 15.1818075 8 TTATATA 102800 0.0 14.554999 4 CGGACAG 8900 0.0 14.146433 1 >>END_MODULE