FastQCFastQC Report
Fri 27 May 2016
SRR1233107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72820238
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC4303070.5909167723401288No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG3646960.500816819631927No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA2082910.2860344949710271No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG1926270.2645239912563867No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG1911130.262444898902967No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1815310.24928646896210366No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG1625200.2231797155071094No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT1466900.2014412531856872No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT1353720.18589887058594892No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT1351300.18556654538811038No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1349360.18530013593199188No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA1171610.16089071282628875No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT1134110.15574104550441045No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT1098250.15081659030007566No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA1081250.1484820744474908No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA1001410.13751808940805715No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA972080.13349036294003871No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA962460.13216930161639956No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC957110.13143461574514492No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG891080.12236708152478161No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT880530.12091830845155985No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT867670.1191523158713104No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG845940.11616825531385931No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC842070.11563680964624147No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG786920.10806336557153245No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA765100.10506694581250889No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG757190.10398070931874737No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT732120.10053798505849432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG377000.031.7165261
TCACGGT809050.029.7049142
CACGGTC952300.024.8837993
CTTTTAT2789950.020.8356571
TTATATA2712300.020.3432944
TATTATA2738000.020.075489
CGCTTCG48600.018.3215086
TTACGCG126050.018.1991238
TTTATAT3083400.018.171793
TTTTATA3168200.018.0679512
TATATAT3092250.017.8204885
GTGAATT1836150.017.2327791
CGGACAG102350.017.1642281
TACGCGC135250.017.1153539
CTGGAAT996100.016.9941221
GGCCAGT716550.016.9311222
ATCACGG1477200.016.6288641
ACGGTCT1486150.015.9075054
GCCAGAA1417250.015.5553361
CGCGCAG137150.015.14677810-11
Produced by FastQC (version 0.11.5)