##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233107_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72820238
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.55503894398148	31.0	26.0	31.0	7.0	34.0
2	26.563190853619567	31.0	26.0	31.0	7.0	34.0
3	26.70582666867966	31.0	26.0	31.0	10.0	34.0
4	30.09416380924215	35.0	28.0	37.0	12.0	37.0
5	29.984429232433982	35.0	29.0	37.0	10.0	37.0
6	30.326198027531852	35.0	30.0	37.0	11.0	37.0
7	29.710144836384632	35.0	30.0	37.0	2.0	37.0
8	29.81207799677886	35.0	30.0	37.0	8.0	37.0
9	31.26297174420111	35.0	30.0	39.0	11.0	39.0
10-11	31.49399552910003	35.0	30.0	39.0	11.0	39.0
12-13	31.358369502994485	35.0	30.0	39.0	10.0	39.0
14-15	31.606059403431228	37.0	30.0	40.0	6.0	41.0
16-17	31.719889098687098	37.0	30.0	40.0	6.0	41.0
18-19	31.729696619502946	37.0	29.0	40.0	10.0	41.0
20-21	31.710827579278167	37.0	29.5	40.0	9.0	41.0
22-23	31.89900845970869	37.0	30.0	40.0	9.0	41.0
24-25	31.652257199434036	37.0	30.0	40.0	8.0	41.0
26-27	31.55547609580732	36.5	29.5	40.0	8.0	41.0
28-29	31.2571424855821	36.0	29.0	40.0	7.5	41.0
30-31	31.13030305531273	36.0	29.0	40.0	2.0	41.0
32-33	30.944133971383067	36.0	28.5	40.0	2.0	41.0
34-35	30.76715727707454	36.0	28.5	40.0	2.0	41.0
36-37	30.699812434010447	36.0	28.5	40.0	2.0	41.0
38-39	30.499799238502902	35.5	28.0	40.0	2.0	41.0
40-41	30.24552902450003	35.0	27.5	39.0	2.0	41.0
42-43	30.06276495553338	35.0	27.5	39.0	2.0	40.0
44-45	29.682964899125984	35.0	27.0	39.0	2.0	40.0
46-47	29.700812314016332	35.0	27.0	39.0	2.0	40.0
48-49	29.822196227922245	35.0	27.5	39.0	2.0	40.0
50-51	29.285983629990334	34.5	26.5	38.5	2.0	40.5
52-53	29.360623732100407	35.0	27.0	39.0	2.0	40.0
54-55	29.554872005499348	35.0	27.0	39.0	2.0	41.0
56-57	29.597579178469587	35.0	27.0	40.0	2.0	41.0
58-59	29.43360901264838	35.0	26.5	39.5	2.0	41.0
60-61	29.09397163052392	34.5	26.0	39.0	2.0	41.0
62-63	28.816400586331508	34.0	26.0	39.0	2.0	40.5
64-65	28.509814524363406	34.0	26.0	39.0	2.0	40.0
66-67	28.177793809188046	34.0	25.0	38.5	2.0	40.0
68-69	27.833498298920695	34.0	24.5	38.0	2.0	40.0
70-71	27.3820660816846	33.0	23.0	37.0	2.0	40.0
72-73	26.928270503592696	33.0	22.0	37.0	2.0	39.0
74-75	26.54896075731035	33.0	21.5	36.0	2.0	39.0
76-77	26.120250911566643	32.0	20.0	36.0	2.0	38.5
78-79	25.744371674533664	32.0	18.5	35.5	2.0	37.5
80-81	25.336451935243606	32.0	17.0	35.0	2.0	37.0
82-83	25.011232928131875	32.0	12.0	35.0	2.0	36.5
84-85	24.707992337240096	32.0	7.5	35.0	2.0	36.0
86-87	24.34919745799238	31.5	4.5	35.0	2.0	36.0
88-89	23.928944437122	31.0	2.0	35.0	2.0	35.5
90-91	23.62559259968362	31.0	2.0	34.5	2.0	35.0
92-93	23.350308007782125	31.0	2.0	34.0	2.0	35.0
94-95	23.031704640954345	31.0	2.0	34.0	2.0	35.0
96-97	22.706180121245964	31.0	2.0	34.0	2.0	35.0
98-99	22.239535608219242	30.5	2.0	34.0	2.0	35.0
100-101	20.656877899794836	27.5	2.0	33.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.244356713012479
1101	2	5.2690794759786606
1101	3	5.116847648907072
1101	4	5.48065076563649
1101	5	5.540758044205731
1101	6	5.069506084279563
1101	7	6.327461543888067
1101	8	6.051889705483255
1101	9	5.86849180813638
1101	10-11	5.349389724294237
1101	12-13	5.428380428487415
1101	14-15	6.4607240201039
1101	16-17	6.092328422970372
1101	18-19	5.984017738974604
1101	20-21	5.9629714723419625
1101	22-23	5.525458708120361
1101	24-25	5.5986631601969705
1101	26-27	5.557717186629148
1101	28-29	5.615038507153329
1101	30-31	5.662017481565023
1101	32-33	5.718030553658437
1101	34-35	5.72785743428156
1101	36-37	5.473547736187708
1101	38-39	5.54289326296767
1101	40-41	5.684369130086068
1101	42-43	5.651506881785767
1101	44-45	5.687445989056105
1101	46-47	5.550215034962381
1101	48-49	5.466759771711541
1101	50-51	5.618352621686157
1101	52-53	5.658406571319496
1101	54-55	5.932765221707626
1101	56-57	5.871734950663466
1101	58-59	5.632509295126507
1101	60-61	5.651896614031855
1101	62-63	5.75413654864462
1101	64-65	5.7257287019677
1101	66-67	5.733694687383171
1101	68-69	5.53372027517111
1101	70-71	5.477500761281011
1101	72-73	5.495792919622961
1101	74-75	5.446058107812881
1101	76-77	5.384636849090235
1101	78-79	5.354248217119775
1101	80-81	5.3642200303526515
1101	82-83	5.294242091395514
1101	84-85	5.339488381157448
1101	86-87	5.217100422397078
1101	88-89	5.131430376644019
1101	90-91	5.053562743179043
1101	92-93	5.056267661062229
1101	94-95	4.992000146322919
1101	96-97	4.881479938264054
1101	98-99	4.8438843678451775
1101	100-101	4.47349859600639
1102	1	5.005192642430323
1102	2	5.1442801717577105
1102	3	4.793442992648462
1102	4	5.153048241369163
1102	5	5.2701612936892275
1102	6	4.811981975699656
1102	7	6.060261184971548
1102	8	5.867358373857961
1102	9	5.600850546142393
1102	10-11	5.083833494474842
1102	12-13	5.1551986037096285
1102	14-15	6.172404111582626
1102	16-17	5.898312289542023
1102	18-19	5.729429243567555
1102	20-21	5.72355605691007
1102	22-23	5.2289886413278595
1102	24-25	5.344713243945932
1102	26-27	5.241724516821719
1102	28-29	5.3103908227180625
1102	30-31	5.229198271690514
1102	32-33	5.36508876368228
1102	34-35	5.338966002434695
1102	36-37	5.123598371637005
1102	38-39	5.334810917111117
1102	40-41	5.3761899835169515
1102	42-43	5.327954641193312
1102	44-45	5.456051338835511
1102	46-47	5.273911904219705
1102	48-49	5.236908661567487
1102	50-51	5.284094334327342
1102	52-53	5.319605312186532
1102	54-55	5.572705839765618
1102	56-57	5.496740274750458
1102	58-59	5.410864388327401
1102	60-61	5.48211035911509
1102	62-63	5.485168541105121
1102	64-65	5.438616793230555
1102	66-67	5.426170694520835
1102	68-69	5.416297970669067
1102	70-71	5.390062598520849
1102	72-73	5.366133228709607
1102	74-75	5.256591046262724
1102	76-77	5.198579814688646
1102	78-79	5.09015619391274
1102	80-81	4.99476705800809
1102	82-83	5.117687175790952
1102	84-85	5.102226261584683
1102	86-87	5.072197238277749
1102	88-89	5.08879762634033
1102	90-91	5.052607108927106
1102	92-93	4.931307561600594
1102	94-95	4.784335469840098
1102	96-97	4.634371984195855
1102	98-99	4.5131485710901025
1102	100-101	4.205300603165298
1103	1	4.945157750878767
1103	2	5.10948372529996
1103	3	4.985298657531018
1103	4	5.433125344745228
1103	5	5.5273959481199455
1103	6	5.0600302121122915
1103	7	6.353024553410894
1103	8	6.010423142821132
1103	9	5.970318309020502
1103	10-11	5.572005919584324
1103	12-13	5.626600880799693
1103	14-15	6.541087359714542
1103	16-17	6.2334778573106355
1103	18-19	5.914982352427284
1103	20-21	6.05714824426218
1103	22-23	5.6569823208517604
1103	24-25	5.837895808117452
1103	26-27	5.757528367294093
1103	28-29	5.849003646526938
1103	30-31	5.6997890389635515
1103	32-33	5.840612706003839
1103	34-35	5.782364204179906
1103	36-37	5.766522109867285
1103	38-39	5.810409390948056
1103	40-41	5.77880777466477
1103	42-43	5.708407845109573
1103	44-45	5.811569432278862
1103	46-47	5.658978515711166
1103	48-49	5.717407255629336
1103	50-51	5.806042967553282
1103	52-53	5.987013941006438
1103	54-55	6.262754018444866
1103	56-57	6.116699659426963
1103	58-59	6.025096008858316
1103	60-61	6.015566301591775
1103	62-63	6.014086673521863
1103	64-65	6.001963316523668
1103	66-67	6.0944674382989845
1103	68-69	6.004362588843438
1103	70-71	5.9015799354392655
1103	72-73	5.869487902820321
1103	74-75	5.880110979416028
1103	76-77	5.703221204839934
1103	78-79	5.685472084810812
1103	80-81	5.729835750199431
1103	82-83	5.743688446995158
1103	84-85	5.770798221533212
1103	86-87	5.727342289949984
1103	88-89	5.712553674846074
1103	90-91	5.655280494616395
1103	92-93	5.64674119422331
1103	94-95	5.475261412884485
1103	96-97	5.324852687749068
1103	98-99	5.150319229984213
1103	100-101	4.88713309023554
1104	1	4.23633596869675
1104	2	4.380704766720484
1104	3	3.487500206128079
1104	4	4.365155983255647
1104	5	2.7999092979834828
1104	6	3.7944890353331253
1104	7	4.250677453293353
1104	8	4.026851346592494
1104	9	4.545902230051599
1104	10-11	4.391822392394165
1104	12-13	4.347217232174479
1104	14-15	5.378704006601939
1104	16-17	4.887588538050078
1104	18-19	4.087105948823083
1104	20-21	2.202398743769578
1104	22-23	3.0542033595689446
1104	24-25	4.339030478652589
1104	26-27	4.44076243220767
1104	28-29	4.583125819784577
1104	30-31	4.590295648258412
1104	32-33	4.671229557906329
1104	34-35	4.559250318210189
1104	36-37	4.365925410317804
1104	38-39	4.369063636963748
1104	40-41	4.380736610839037
1104	42-43	4.454057033127167
1104	44-45	4.555866698775507
1104	46-47	4.3904265765052735
1104	48-49	4.222562648228628
1104	50-51	4.32501677392456
1104	52-53	4.447893269005434
1104	54-55	4.604568574567253
1104	56-57	4.367226657536374
1104	58-59	4.405529185763978
1104	60-61	4.50076998882891
1104	62-63	4.563022012347048
1104	64-65	4.472804447836413
1104	66-67	4.44625161888758
1104	68-69	4.43217133772934
1104	70-71	4.444895195863076
1104	72-73	4.3534941598584105
1104	74-75	4.1361875737378995
1104	76-77	4.1583300873350275
1104	78-79	4.1790033381096165
1104	80-81	4.041859419106011
1104	82-83	4.097113394211334
1104	84-85	4.117042139973677
1104	86-87	4.159811157374747
1104	88-89	4.225190406758028
1104	90-91	4.124842530542072
1104	92-93	3.8977835617039993
1104	94-95	3.761788616281681
1104	96-97	3.685231218499407
1104	98-99	3.622292943841753
1104	100-101	3.4668548542567343
1105	1	0.29797308955921054
1105	2	-0.4881541147818602
1105	3	1.3048124164710018
1105	4	1.0638610713555714
1105	5	1.3073280918474879
1105	6	-1.9904992993232646
1105	7	0.10817488768846673
1105	8	1.8057021654430763
1105	9	-0.4058747815806285
1105	10-11	-2.26159977749575
1105	12-13	-2.901675747814096
1105	14-15	-1.669778390120296
1105	16-17	-0.7425599128486802
1105	18-19	-0.5674100273654723
1105	20-21	-1.8384129110749647
1105	22-23	-2.493900568386138
1105	24-25	-2.663116720903421
1105	26-27	-1.8585137577930766
1105	28-29	-1.5061192935118832
1105	30-31	-1.4160309368307153
1105	32-33	-2.076768305319902
1105	34-35	-1.0912928797143522
1105	36-37	-2.081539922665602
1105	38-39	-2.518455463291893
1105	40-41	-2.4193866717681445
1105	42-43	-2.208033313314907
1105	44-45	-2.194760460055644
1105	46-47	-2.051180481195793
1105	48-49	-1.641357386513885
1105	50-51	-0.10964285781707872
1105	52-53	-1.3234593956865837
1105	54-55	-0.7533275637684156
1105	56-57	0.28647683530412493
1105	58-59	-1.5344671303501514
1105	60-61	-1.4652755449865822
1105	62-63	-1.6028103972476018
1105	64-65	-0.6921945332249813
1105	66-67	-0.8406921317291953
1105	68-69	-1.3636764800840986
1105	70-71	-1.1688675575302057
1105	72-73	-0.9290235875844282
1105	74-75	-1.1544573099487074
1105	76-77	-1.6917252404084842
1105	78-79	-1.311666644164351
1105	80-81	-0.9241993731952256
1105	82-83	-0.935976751108349
1105	84-85	-0.798282953963799
1105	86-87	-1.0337714649277707
1105	88-89	-0.8517803815836551
1105	90-91	-0.9860466758750519
1105	92-93	-0.8758686367691944
1105	94-95	-0.9471213779256971
1105	96-97	-0.6026799474371423
1105	98-99	-0.5335618129371902
1105	100-101	-1.1061253961707784
1106	1	2.3340239476575455
1106	2	2.2692543803618612
1106	3	3.042529469869592
1106	4	1.8970951099579771
1106	5	0.40648668068248384
1106	6	0.9116595020097726
1106	7	3.7071019002397207
1106	8	2.6001350579474476
1106	9	1.6201853157999437
1106	10-11	2.229071968959321
1106	12-13	2.648753736693841
1106	14-15	2.8242396715185727
1106	16-17	3.3554537518574357
1106	18-19	1.4645530476148316
1106	20-21	1.37377771855882
1106	22-23	2.1638448825933096
1106	24-25	2.946273730523316
1106	26-27	2.0194430731158306
1106	28-29	3.1851703193090586
1106	30-31	2.417948769216519
1106	32-33	1.6347321266195038
1106	34-35	0.8658199246705536
1106	36-37	1.7798569114837584
1106	38-39	3.053255366432655
1106	40-41	3.114490947181828
1106	42-43	2.407248837459676
1106	44-45	1.945279762590868
1106	46-47	3.093828296051523
1106	48-49	3.305941569618934
1106	50-51	3.3513300886336346
1106	52-53	3.4668508835688314
1106	54-55	3.5528740505574703
1106	56-57	3.4644240860597577
1106	58-59	3.366063955328787
1106	60-61	3.207001227077587
1106	62-63	3.363586687484311
1106	64-65	3.4207929530491903
1106	66-67	3.3867544165184
1106	68-69	3.144379837598212
1106	70-71	3.138795954225202
1106	72-73	3.0180895505710446
1106	74-75	3.044059519586046
1106	76-77	3.1048198968047167
1106	78-79	3.0063159926249554
1106	80-81	2.9839959211424407
1106	82-83	2.9470149335318183
1106	84-85	3.0254321060322944
1106	86-87	2.9699088905342244
1106	88-89	3.0074288634848827
1106	90-91	2.8659733727778303
1106	92-93	2.7655098809624796
1106	94-95	2.6435832533062573
1106	96-97	2.5544956943568344
1106	98-99	2.606755195371086
1106	100-101	2.6000930956413093
1107	1	1.0316717411998617
1107	2	0.2881163692694564
1107	3	-1.04084480752336
1107	4	-0.17236953254107235
1107	5	-0.33112550697299525
1107	6	0.1238819775099671
1107	7	0.5774350303667752
1107	8	0.4379905501168153
1107	9	0.6454516000237795
1107	10-11	0.6624019193649566
1107	12-13	0.7784786532834822
1107	14-15	-1.154061173850959
1107	16-17	-1.3778617532707784
1107	18-19	-0.9151062152614458
1107	20-21	-2.3983692400083747
1107	22-23	-1.0697805151681834
1107	24-25	-3.305950156070274
1107	26-27	-4.649694160200564
1107	28-29	-2.9085525055400154
1107	30-31	-1.8760290219826317
1107	32-33	-1.1604191695424078
1107	34-35	-2.258475091257978
1107	36-37	-0.5861855094454604
1107	38-39	-1.635536984207345
1107	40-41	-0.20294372011957762
1107	42-43	-0.12909371650404466
1107	44-45	-0.6558212391536777
1107	46-47	-0.8640646536214227
1107	48-49	0.4150002827932937
1107	50-51	0.5165001279815229
1107	52-53	0.5900262590631726
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2208	28-29	3.005646196282285
2208	30-31	2.8236452468668105
2208	32-33	1.8394189634135678
2208	34-35	2.2387411691923376
2208	36-37	2.159750285823609
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2208	50-51	1.4774115382921806
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2208	64-65	2.039529199526406
2208	66-67	2.2641562788183833
2208	68-69	2.2732912184133234
2208	70-71	1.7057494821758752
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2208	74-75	2.541518338671157
2208	76-77	2.6876942983677985
2208	78-79	1.7855033836383
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2208	82-83	2.4581345603155356
2208	84-85	2.172772657829917
2208	86-87	2.6456859433779236
2208	88-89	2.739826621743152
2208	90-91	2.8273554607979534
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2208	94-95	2.5962462663935177
2208	96-97	2.3329073475985247
2208	98-99	2.3267352883149464
2208	100-101	1.8501202079983052
2209	1	3.1808856344534853
2209	2	2.7656927201401125
2209	3	3.4380228671638626
2209	4	3.360884290483405
2209	5	3.625532376181461
2209	6	3.4339186161625825
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2209	9	4.569168715821608
2209	10-11	3.5337865260213555
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2209	14-15	5.079638062381992
2209	16-17	4.807604095473696
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2209	22-23	0.1776693114882626
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2209	26-27	3.5864882374098883
2209	28-29	3.960491227242329
2209	30-31	3.96849514101374
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2209	34-35	3.454898854528821
2209	36-37	3.407741249137164
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2313	46-47	-25.7937696188409
2313	48-49	-25.92413620077591
2313	50-51	-25.40089493078557
2313	52-53	-25.424874196681607
2313	54-55	-25.58379501816311
2313	56-57	-25.63131959919007
2313	58-59	-25.51350423648614
2313	60-61	-25.20818599274778
2313	62-63	-24.911599783648928
2313	64-65	-24.618536780322824
2313	66-67	-24.279249345659544
2313	68-69	-23.947274116900022
2313	70-71	-23.493530700150437
2313	72-73	-23.06284406851312
2313	74-75	-22.704431397842573
2313	76-77	-22.296156210557736
2313	78-79	-21.93849603885755
2313	80-81	-21.545245185798873
2313	82-83	-21.223134711307353
2313	84-85	-20.920385980497798
2313	86-87	-20.568934192954305
2313	88-89	-20.166628018399575
2313	90-91	-19.88729045696958
2313	92-93	-19.63122207789689
2313	94-95	-19.32786350064543
2313	96-97	-19.0311780817655
2313	98-99	-18.59460555257235
2313	100-101	-17.146657112588688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5041555.0
3	471812.0
4	365931.0
5	333754.0
6	370213.0
7	385656.0
8	483846.0
9	553812.0
10	511656.0
11	535325.0
12	543151.0
13	574749.0
14	632588.0
15	693203.0
16	743527.0
17	778293.0
18	797168.0
19	800720.0
20	797120.0
21	815854.0
22	860632.0
23	963158.0
24	1166365.0
25	1450996.0
26	1760029.0
27	1935477.0
28	2046954.0
29	2239447.0
30	2542872.0
31	2864745.0
32	3286772.0
33	3856531.0
34	4550823.0
35	5408201.0
36	6532463.0
37	8011697.0
38	6186566.0
39	925399.0
40	1178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.43827326335908	17.412475061725186	14.907658511235786	32.24159316367995
2	21.23649216344453	19.857614264673764	41.5080855596964	17.39780801218531
3	22.995838512416455	22.857765778296553	31.229993771484605	22.916401937802387
4	23.128369910942013	30.45064706448996	27.421176582462238	18.999806442105786
5	22.613800191146925	32.411502649722465	29.59410554824548	15.380591610885133
6	18.264258561155128	37.53503457335297	27.972906684983474	16.22780018050842
7	16.767087981970892	19.918438537050783	46.067399571806675	17.24707390917165
8	18.230385676278825	25.01250347205357	36.47925303580514	20.277857815862472
9	18.941752752507877	24.528195602422358	35.99490757880357	20.53514406626619
10-11	19.816676409251325	35.30032848296468	27.274107012250997	17.608888095533
12-13	19.66017100160412	29.670584827276613	33.0948843293719	17.574359841747366
14-15	20.66981746803448	28.758470980338895	32.894482145125856	17.67722940650076
16-17	20.355272762878798	28.73253748431754	32.36518708566293	18.547002667140728
18-19	20.0353520641842	29.838922671312023	30.785543070907888	19.340182193595894
20-21	20.75174156502479	30.279556014809422	29.511267395445824	19.457435024719967
22-23	20.57109842564612	29.81191892049887	31.35453410190631	18.2624485519487
24-25	19.811694283801344	29.434877893962856	31.997941720669992	18.75548610156581
26-27	20.15774071852288	29.67826731713411	30.6306711286154	19.533320835727608
28-29	20.992146165041955	28.752575295549903	29.82019230787455	20.435086231533592
30-31	19.84305616457127	29.363149857200412	30.788329455604835	20.005464522623488
32-33	19.98317841556822	29.339876073147536	31.37842228892001	19.298523222364235
34-35	19.195586514609904	32.32918328075255	29.231625669580293	19.243604535057244
36-37	19.88895049137178	30.489507280583865	29.698260330441684	19.923281897602667
38-39	19.982302411294338	29.905709984484137	31.222323080446657	18.889664523774865
40-41	20.700024461864274	30.204612845620066	30.43791232683523	18.657450365680425
42-43	20.104358111881904	30.097152348878186	31.430051612826215	18.368437926413698
44-45	19.971756868188645	30.577383256521934	30.329803499706447	19.121056375582974
46-47	20.380429241498078	29.616620678825246	30.995633671859064	19.007316407817612
48-49	21.229908580632042	29.61424935463441	30.425400875294194	18.730441189439354
50-51	20.14638479914646	29.985536457015442	30.33298917100284	19.535089572835258
52-53	19.529696927386137	29.64410531689766	31.0284966379221	19.7977011177941
54-55	19.38250656262235	29.669414644755506	31.388400769993424	19.559678022628717
56-57	19.40898026745695	29.658450906905525	30.842501834871417	20.090066990766108
58-59	20.02838121633289	29.71866341318446	30.61106936792956	19.641886002553093
60-61	19.80062304416342	29.535090191308612	31.237672770267842	19.426613994260126
62-63	19.24916497022754	29.81652413168766	31.613277632173098	19.3210332659117
64-65	19.310992114103296	30.400262260074545	30.91980122303881	19.36894440278335
66-67	18.79707707113802	30.974000693489906	30.70451454089583	19.52440769447625
68-69	19.360045097472245	30.491529112621752	31.57718325730754	18.571242532598465
70-71	19.64296605275037	30.90206373139971	30.583656030643308	18.871314185206614
72-73	19.26557392724665	31.13504936178988	30.199761427786818	19.39961528317665
74-75	18.68186565489388	29.311364144751767	32.22140476745772	19.785365432896633
76-77	18.96194315194174	29.942809058833227	31.46235433759179	19.63289345163325
78-79	18.915512594685772	31.12141840028161	29.96535328952874	19.99771571550388
80-81	19.29247457611361	31.92753743774442	30.01112882589621	18.768859160245768
82-83	19.785662627308405	30.65089003115072	30.625327734507028	18.938119607033848
84-85	19.232559706903064	31.374547245421198	30.493168671799666	18.899724375876072
86-87	20.344332163898912	31.99933885381199	28.728434065942555	18.927894916346546
88-89	19.937452006613018	31.06344953153793	29.902314540974444	19.09678392087461
90-91	20.012548625460173	31.396209916035705	29.609824635647392	18.98141682285673
92-93	19.568858990594688	31.12837941958627	30.12592232078253	19.176839269036513
94-95	19.492235818908814	32.24604476785659	29.669622306190806	18.59209710704379
96-97	19.52177439450062	31.411395499100948	30.182381123304268	18.884448983094163
98-99	19.475067824298126	31.430246244570114	29.8612111065352	19.23347482459656
100-101	20.65656303303414	32.055432632468026	28.481495248696	18.80650908580184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5204.0
1	6023.5
2	8153.0
3	13074.0
4	21175.0
5	30543.5
6	40239.5
7	51813.0
8	66820.0
9	78784.5
10	96787.0
11	124941.5
12	145825.0
13	175024.0
14	200846.5
15	286950.5
16	583635.5
17	793763.5
18	962502.5
19	1066204.0
20	1019528.0
21	857597.0
22	580284.0
23	539023.5
24	722796.5
25	913544.5
26	1001813.5
27	896549.5
28	814898.0
29	892422.5
30	1032671.5
31	1243361.5
32	1523573.5
33	1866320.0
34	2149156.5
35	2498464.5
36	3090978.0
37	3569554.0
38	3563717.0
39	3302131.0
40	3036512.0
41	2908501.0
42	2762721.0
43	2546242.5
44	2367853.0
45	2256943.5
46	2187545.0
47	2091917.5
48	2024159.0
49	1928080.5
50	1754444.5
51	1651580.0
52	1562483.5
53	1290630.5
54	1019317.0
55	865269.5
56	747727.5
57	632904.5
58	521738.5
59	418080.0
60	326218.5
61	250261.5
62	189684.0
63	143791.5
64	108490.5
65	81453.5
66	60723.0
67	46089.5
68	35646.5
69	27457.5
70	22244.5
71	19898.0
72	17512.5
73	16502.0
74	15486.5
75	12379.5
76	10104.5
77	8156.5
78	6306.5
79	4568.5
80	3107.0
81	2015.0
82	1232.5
83	738.0
84	456.0
85	272.0
86	179.5
87	142.0
88	111.5
89	77.5
90	55.5
91	32.0
92	25.0
93	21.5
94	16.0
95	13.0
96	7.5
97	6.0
98	6.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	6.6202022025800025
2	7.062146652143597
3	4.1850893154180575
4	2.262741849319416
5	3.466857661190286
6	1.5889799755941472
7	6.993136990296571
8	5.739206729865398
9	2.9014063370680003
10-11	1.9908174428103351
12-13	3.364237150666824
14-15	4.709149673474014
16-17	4.653608108229474
18-19	2.8895003062198175
20-21	2.8914173557081755
22-23	1.7250444855728155
24-25	1.8763732961158408
26-27	2.56519485695721
28-29	2.1852132644773836
30-31	1.771134694725936
32-33	2.3504420570556226
34-35	2.120113916683436
36-37	1.4828343461332822
38-39	2.888289791088021
40-41	2.2521953306442093
42-43	2.9054011056651587
44-45	2.9689514335286846
46-47	2.0134965776958875
48-49	2.816059183986737
50-51	2.1033829908658084
52-53	2.592363128502821
54-55	2.030108717853957
56-57	1.6966732517408143
58-59	1.396262258851722
60-61	1.756271381590376
62-63	1.716193237379971
64-65	1.1113352856660534
66-67	0.8497012877107049
68-69	0.7188976778680675
70-71	0.9855920822450484
72-73	0.21692046653294375
74-75	0.4648206450519977
76-77	0.9564518314263131
78-79	0.7166798878081118
80-81	0.7420684343272814
82-83	0.7925276212362833
84-85	0.7509052634516246
86-87	0.005863067901535833
88-89	0.4314116633345802
90-91	0.002614657754895006
92-93	0.2629681051028699
94-95	0.679445733204003
96-97	0.007731367205913279
98-99	0.2873878275432168
100-101	0.2303782638007857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7.2820238E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.003912960584366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96568830952522	30.0100514538783
2	9.571180706374259	7.274846369901435
3	3.833659903591497	4.370822318897204
4	2.0515363505255824	3.118656356033964
5	1.2243052763563125	2.326419557991474
6	0.8233518170266364	1.877435447413156
7	0.5842264785583026	1.5542024568278907
8	0.4476350778502865	1.3609507629381357
9	0.3322409306606621	1.1363809869694212
>10	1.9314123055333206	13.46336442573915
>50	0.1388391455309695	3.6184177909202946
>100	0.0807013414356369	5.779355011589478
>500	0.0072684275010695175	1.9025549281720893
>1k	0.005841117508992838	4.566504987904341
>5k	9.294865087399296E-4	2.5017463225332612
>10k+	0.0011833255129681014	15.138290822290562
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC	430307	0.5909167723401288	No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG	364696	0.500816819631927	No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA	208291	0.2860344949710271	No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG	192627	0.2645239912563867	No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG	191113	0.262444898902967	No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA	181531	0.24928646896210366	No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG	162520	0.2231797155071094	No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT	146690	0.2014412531856872	No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT	135372	0.18589887058594892	No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT	135130	0.18556654538811038	No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC	134936	0.18530013593199188	No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA	117161	0.16089071282628875	No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT	113411	0.15574104550441045	No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT	109825	0.15081659030007566	No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA	108125	0.1484820744474908	No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA	100141	0.13751808940805715	No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA	97208	0.13349036294003871	No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA	96246	0.13216930161639956	No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC	95711	0.13143461574514492	No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG	89108	0.12236708152478161	No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT	88053	0.12091830845155985	No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT	86767	0.1191523158713104	No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG	84594	0.11616825531385931	No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC	84207	0.11563680964624147	No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG	78692	0.10806336557153245	No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA	76510	0.10506694581250889	No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG	75719	0.10398070931874737	No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT	73212	0.10053798505849432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0016561330107160595	0.0	0.0	1.3732446191675452E-6	0.0
2	0.0016684922122885673	2.7464892383350905E-6	0.0	1.0985956953340362E-5	0.0
3	0.0016890908815760804	2.7464892383350905E-6	0.0	1.0985956953340362E-5	0.0
4	0.0017838447602986412	2.7464892383350905E-6	0.0	1.5105690810842996E-5	0.0
5	0.0018209223650161649	4.119733857502635E-6	0.0	1.922542466834563E-5	2.7464892383350905E-6
6	0.0018634929482103588	5.492978476670181E-6	0.0	3.158462624085354E-5	2.7464892383350905E-6
7	0.00189645081907038	6.866223095837726E-6	0.0	4.394382781336145E-5	2.7464892383350905E-6
8	0.0019706060285054274	8.23946771500527E-6	0.0	4.806356167086408E-5	2.7464892383350905E-6
9	0.0020900783103730038	8.23946771500527E-6	0.0	5.7676274005036896E-5	4.119733857502635E-6
10-11	0.0023056777155823086	9.612712334172815E-6	0.0	6.110938555295576E-5	4.119733857502635E-6
12-13	0.002642809269587941	1.0985956953340362E-5	0.0	6.934885326796104E-5	4.119733857502635E-6
14-15	0.0031049060839378195	1.0985956953340362E-5	0.0	8.308129945963648E-5	4.119733857502635E-6
16-17	0.0038389053328828724	1.1672579262924134E-5	0.0	9.956023488964701E-5	4.119733857502635E-6
18-19	0.004769278562368884	1.2359201572507907E-5	0.0	1.1260605877173871E-4	6.179600786253953E-6
20-21	0.005641975517849859	1.2359201572507907E-5	0.0	1.2496526034424662E-4	8.23946771500527E-6
22-23	0.006635517999817578	1.2359201572507907E-5	0.0	1.3526459498800321E-4	8.23946771500527E-6
24-25	0.007631120348714049	1.2359201572507907E-5	0.0	1.428174403934247E-4	1.0299334643756588E-5
26-27	0.008739328756382257	1.3732446191675452E-5	6.866223095837726E-7	1.5860975351385145E-4	1.0985956953340362E-5
28-29	0.009885301391077574	1.5105690810842996E-5	1.3732446191675452E-6	1.6684922122885674E-4	1.0985956953340362E-5
30-31	0.011192630268525077	1.7852180049178086E-5	1.3732446191675452E-6	1.7371544432469446E-4	1.0985956953340362E-5
32-33	0.012762935490543165	1.7852180049178086E-5	1.3732446191675452E-6	1.757753112534458E-4	1.304582388209168E-5
34-35	0.014299596219391648	1.922542466834563E-5	2.059866928751318E-6	1.8332815665886727E-4	1.3732446191675452E-5
36-37	0.015997613190992317	2.0598669287513176E-5	2.7464892383350905E-6	1.9088100206428877E-4	1.3732446191675452E-5
38-39	0.01806365972052989	2.1971913906680724E-5	2.7464892383350905E-6	1.9706060285054275E-4	1.3732446191675452E-5
40-41	0.020352858500682188	2.2658536216264498E-5	2.7464892383350905E-6	2.1628602751888837E-4	1.922542466834563E-5
42-43	0.023110333695970618	2.403178083543204E-5	2.7464892383350905E-6	2.3001847371056382E-4	1.99120469779294E-5
44-45	0.02597698183848287	2.4718403145015813E-5	2.7464892383350905E-6	2.3688469680640156E-4	2.0598669287513176E-5
46-47	0.029145057174902393	2.609164776418336E-5	2.7464892383350905E-6	2.4855727606932566E-4	2.0598669287513176E-5
48-49	0.03309862843348575	2.7464892383350903E-5	2.7464892383350905E-6	2.6503621149933623E-4	2.1971913906680724E-5
50-51	0.037878206330498404	2.7464892383350903E-5	2.7464892383350905E-6	2.6915594535683885E-4	2.2658536216264498E-5
52-53	0.043423368102696945	2.8151514692934677E-5	2.7464892383350905E-6	2.725890569047577E-4	2.334515852584827E-5
54-55	0.048934198759416306	3.0211381621685993E-5	2.7464892383350905E-6	2.8151514692934676E-4	2.334515852584827E-5
56-57	0.05532459259471248	3.0211381621685993E-5	2.7464892383350905E-6	2.870081254060169E-4	2.334515852584827E-5
58-59	0.062337066242491544	3.158462624085354E-5	2.7464892383350905E-6	2.925011038826871E-4	2.334515852584827E-5
60-61	0.07047354061105926	3.158462624085354E-5	2.7464892383350905E-6	3.0760679469353015E-4	2.334515852584827E-5
62-63	0.08032931724282472	3.295787086002109E-5	2.7464892383350905E-6	3.172195070277029E-4	2.4718403145015813E-5
64-65	0.09161670139007236	3.433111547918863E-5	2.7464892383350905E-6	3.323251978385459E-4	2.5405025454599588E-5
66-67	0.10607422074066827	3.433111547918863E-5	2.7464892383350905E-6	3.460576440302214E-4	2.609164776418336E-5
68-69	0.12448805783908588	3.433111547918863E-5	2.7464892383350905E-6	3.542971117452266E-4	2.7464892383350903E-5
70-71	0.1461551114403114	3.433111547918863E-5	2.7464892383350905E-6	3.6116333484106434E-4	2.8838137002518448E-5
72-73	0.1735575211934902	3.570436009835617E-5	2.7464892383350905E-6	3.680295579369021E-4	2.952475931210222E-5
74-75	0.2107072212535202	3.7764227027107494E-5	2.7464892383350905E-6	3.8244862643816135E-4	3.089800393126977E-5
76-77	0.258256502814506	3.9137471646275036E-5	2.7464892383350905E-6	4.112867634406798E-4	3.158462624085354E-5
78-79	0.32003191200775805	4.051071626544258E-5	2.7464892383350905E-6	4.3394529965694426E-4	3.158462624085354E-5
80-81	0.40115359139584245	4.25705831941939E-5	2.7464892383350905E-6	4.655299258977978E-4	3.158462624085354E-5
82-83	0.5015013820745821	4.394382781336145E-5	2.7464892383350905E-6	4.7994899439905703E-4	3.158462624085354E-5
84-85	0.6358809209055318	4.394382781336145E-5	2.7464892383350905E-6	4.929948182811486E-4	3.158462624085354E-5
86-87	0.8098497288624626	4.394382781336145E-5	2.7464892383350905E-6	5.10160376020743E-4	3.295787086002108E-5
88-89	1.0298071258706956	4.394382781336145E-5	2.7464892383350905E-6	5.149667321878294E-4	3.295787086002108E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCCAG	37700	0.0	31.716526	1
TCACGGT	80905	0.0	29.704914	2
CACGGTC	95230	0.0	24.883799	3
CTTTTAT	278995	0.0	20.835657	1
TTATATA	271230	0.0	20.343294	4
TATTATA	273800	0.0	20.07548	9
CGCTTCG	4860	0.0	18.321508	6
TTACGCG	12605	0.0	18.199123	8
TTTATAT	308340	0.0	18.17179	3
TTTTATA	316820	0.0	18.067951	2
TATATAT	309225	0.0	17.820488	5
GTGAATT	183615	0.0	17.232779	1
CGGACAG	10235	0.0	17.164228	1
TACGCGC	13525	0.0	17.115353	9
CTGGAAT	99610	0.0	16.994122	1
GGCCAGT	71655	0.0	16.931122	2
ATCACGG	147720	0.0	16.628864	1
ACGGTCT	148615	0.0	15.907505	4
GCCAGAA	141725	0.0	15.555336	1
CGCGCAG	13715	0.0	15.146778	10-11
>>END_MODULE