FastQCFastQC Report
Fri 27 May 2016
SRR1233107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72820238
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC5817230.7988479795960018No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG3804110.5223973588221451No Hit
ATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAG2499550.34324935878402374No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG2484670.3412059707907025No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT2470350.3392394844960545No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA1828870.25114858866569484No Hit
ATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTA1809590.2485009730399398No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA1708250.23458451206929593No Hit
ATTATTTGATACTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTT1705220.23416841894968815No Hit
GCCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGG1608460.22088090401462296No Hit
TATTAATAAAAAACTAACAAAAGTTAATTATCTTAACGGGAAGTGGCGGC1496300.20547859236603977No Hit
GTCGTATTACAATGCCTATAGTGAGTCGTATTACAATGCCTATAGTGAGT1466760.20142202776101883No Hit
GTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGA1374400.1887387404583874No Hit
GCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTGTT1369920.18812352686900033No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC1339680.1839708351406377No Hit
GCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAGGT1337050.18360967180579663No Hit
AGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAA1331840.18289421135921033No Hit
GTCGTATTACAATTGCCTATAGTGAGTCGTATTACAATTGCCTATAGTGA1175130.16137409493223573No Hit
ACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTAC1113390.1528956826534953No Hit
GGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGG1107130.15203603152189643No Hit
GTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAA1072320.1472557670025742No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1011950.13896548923665972No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT989980.13594847080834863No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT989290.13585371692962608No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT954740.13110915677040222No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT941060.129230558131381No Hit
CTGCTAGAAAGGCTGAAAAGGCTAAGTCCGCTGGTGTCCAAGGTTCCAAG941010.12922369190828517No Hit
CAACAAGGAAAAGAAGAGAGCTGAAAAGGCTGCTAGAAAGGCTGAAAAGG885410.12158845182571362No Hit
ATACGACTCACTATAGGCATTGTAATACGACTCACTATAGGCATTGTAAT882110.12113528110138831No Hit
CCAGAAGTTAGAAAAGCTAACAGAGAAGAAAAATTGAAAGCCAACAAGGA848310.11649371428860203No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT847750.11641681258992864No Hit
ATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATG844430.11596089537636502No Hit
CGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAATATATCATTAC823980.11315261013016738No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG802200.11016168334962047No Hit
ATTGTATCACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAA743180.10205679360729361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAG367050.033.2312931
TCACGGT949000.029.3780082
CACGGTC1087400.025.6039893
GTCGAAT98850.024.0769771
GTCGGAT136350.023.895251
GTGCGTT72350.022.151591
CGGATTA141300.022.0797753
CGCTTCG57750.021.8382386
CTTTTAT3224450.021.4826681
TATTATA3308100.021.0466639
CGAATTA101150.021.0001813
TTATATA3185350.020.8124434
GCGTTCC70550.020.5653113
TGCGTTC76350.019.3135682
TTTTATA3554500.019.2886122
TCGGATT169450.019.1672692
TTTATAT3545150.018.9516123
TTACGCG156700.018.7590438
TATATAT3622750.018.537625
GTGAATT2129450.018.0493681
Produced by FastQC (version 0.11.5)