FastQCFastQC Report
Fri 27 May 2016
SRR1233106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44661688
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1095870.2453713796039236No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG757870.16969130230814383No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC686730.15376266118736936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT778000.019.2849391
GATCGGG52600.018.3880421
CGCCGGA36800.018.1828346-47
GCGAACT30900.018.0406671
GGAAAAC1480650.017.836831
CGGCGAG40450.017.6684281
ATCGGGA54100.017.5259282
CGACGGC39500.016.5252741
ATCCGGT64300.016.5250236
TCCGGTA60400.016.4072577
TATTATA471200.016.2488549
GCCGGAT48550.016.17703646-47
CTTTATT1155850.015.841421
CGGGAGT62600.015.8312131
CGCAGTT59700.015.7223381
CCGGTAA62600.015.6643938
TTATATA523800.015.2819514
GAGCGGC93200.014.7554989
GGCGCCG56400.014.68309144-45
CTGGAAT809450.014.5977821
Produced by FastQC (version 0.11.5)