##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44661688 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3977560140584 33.0 31.0 34.0 28.0 34.0 2 31.542247059717045 33.0 31.0 34.0 28.0 34.0 3 31.713831080455357 34.0 31.0 34.0 30.0 34.0 4 35.1396875998059 37.0 35.0 37.0 33.0 37.0 5 35.10413670437176 37.0 35.0 37.0 32.0 37.0 6 35.11299911010977 37.0 35.0 37.0 32.0 37.0 7 35.009451837109246 37.0 35.0 37.0 32.0 37.0 8 35.04507986800678 37.0 35.0 37.0 32.0 37.0 9 36.68817582532931 39.0 37.0 39.0 33.0 39.0 10-11 36.74373177520742 39.0 37.0 39.0 33.0 39.0 12-13 36.648619091602626 39.0 37.0 39.0 32.5 39.0 14-15 37.82237397744572 40.0 38.0 41.0 32.0 41.0 16-17 37.75839350272654 40.0 38.0 41.0 32.0 41.0 18-19 37.790049964076594 40.0 38.0 41.0 32.0 41.0 20-21 37.82209270728863 40.0 38.0 41.0 32.0 41.0 22-23 37.76229031020951 40.0 38.0 41.0 32.0 41.0 24-25 37.07297027152221 40.0 37.0 41.0 30.5 41.0 26-27 36.045016267634125 39.0 35.0 41.0 27.0 41.0 28-29 35.7135473249466 39.0 34.5 41.0 27.0 41.0 30-31 35.53616101567859 39.0 34.0 41.0 26.0 41.0 32-33 35.44339092154331 39.0 34.0 41.0 25.0 41.0 34-35 35.2086229365088 38.5 34.0 41.0 25.0 41.0 36-37 35.117860883359356 38.0 34.0 41.0 25.0 41.0 38-39 35.037066590496984 38.0 34.0 40.5 25.0 41.0 40-41 35.03333901083184 38.5 34.0 40.5 24.5 41.0 42-43 35.083192276565995 39.0 34.0 41.0 25.0 41.0 44-45 35.00506803504605 39.0 34.0 41.0 24.0 41.0 46-47 34.91105210801705 38.0 34.0 41.0 24.0 41.0 48-49 34.80018982936785 38.5 33.0 41.0 24.0 41.0 50-51 34.68018358374632 38.0 33.0 41.0 24.0 41.0 52-53 34.54189232614763 38.0 33.0 40.5 23.5 41.0 54-55 34.37831723915137 38.0 33.0 40.0 23.0 41.0 56-57 34.17787435620436 38.0 33.0 40.0 22.5 41.0 58-59 33.97057214899715 38.0 33.0 40.0 22.0 41.0 60-61 33.73158280537896 38.0 32.5 40.0 21.5 41.0 62-63 33.487847895493786 37.5 32.0 40.0 20.5 41.0 64-65 33.19147744706828 37.0 32.0 40.0 19.5 41.0 66-67 32.82611407343135 36.5 31.0 40.0 18.0 41.0 68-69 32.51183725971127 36.0 31.0 39.0 16.5 41.0 70-71 32.184067561441026 36.0 31.0 39.0 13.0 41.0 72-73 31.89461699253284 35.0 31.0 39.0 13.0 40.5 74-75 31.50598563359271 35.0 30.5 38.0 12.0 40.0 76-77 31.13879702665963 35.0 30.0 37.0 8.0 39.5 78-79 30.787851480221704 35.0 30.0 37.0 7.5 39.0 80-81 30.435922507004214 35.0 30.0 36.0 7.0 39.0 82-83 30.10821428200385 34.5 30.0 36.0 4.0 37.5 84-85 29.778036255593385 34.0 29.0 35.5 2.0 37.0 86-87 29.445685259365924 34.0 29.0 35.0 2.0 37.0 88-89 29.14734878583183 34.0 29.0 35.0 2.0 36.0 90-91 28.87675031449774 34.0 29.0 35.0 2.0 36.0 92-93 28.61093144307488 34.0 29.0 35.0 2.0 36.0 94-95 28.340965124291763 34.0 29.0 35.0 2.0 35.5 96-97 28.011738606924126 34.0 29.0 35.0 2.0 35.0 98-99 27.68583401952922 34.0 28.0 35.0 2.0 35.0 100-101 26.639579979601308 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25351671801596254 1101 2 0.3627840471141788 1101 3 0.34752019300374215 1101 4 0.32400967052638663 1101 5 0.30385115578621225 1101 6 0.325139706913518 1101 7 0.35612542928543434 1101 8 0.3409247502086643 1101 9 0.4115800101310896 1101 10-11 0.3808117324705549 1101 12-13 0.3873334781273954 1101 14-15 0.5363221830030085 1101 16-17 0.44415312470458446 1101 18-19 0.47506636894276255 1101 20-21 0.4290751350074018 1101 22-23 0.4708888027357645 1101 24-25 0.5081552739131538 1101 26-27 0.44463862273937593 1101 28-29 0.3567161698514383 1101 30-31 0.287998796315712 1101 32-33 0.2962791674734362 1101 34-35 0.42241864987732214 1101 36-37 0.3282920975558383 1101 38-39 0.35200690413734037 1101 40-41 0.4323270216920605 1101 42-43 0.4129802657369268 1101 44-45 0.33794016302331187 1101 46-47 0.3667102344866393 1101 48-49 0.39175790749452233 1101 50-51 0.4841328080566569 1101 52-53 0.4839476770082882 1101 54-55 0.5139979633549956 1101 56-57 0.5730940977269015 1101 58-59 0.566858962684968 1101 60-61 0.5268322423821843 1101 62-63 0.6110124441125322 1101 64-65 0.5868527640663146 1101 66-67 0.49963797162699564 1101 68-69 0.5486460745046742 1101 70-71 0.5526643063199188 1101 72-73 0.5519352520018437 1101 74-75 0.5186446833309901 1101 76-77 0.5167646400556762 1101 78-79 0.5008772367896022 1101 80-81 0.4425967664970827 1101 82-83 0.4116878387094083 1101 84-85 0.33182168122512934 1101 86-87 0.28574631306620546 1101 88-89 0.4182412916968019 1101 90-91 0.4132645205705998 1101 92-93 0.3907184869094955 1101 94-95 0.48034706643119307 1101 96-97 0.46057595908513704 1101 98-99 0.3758856752681474 1101 100-101 0.4367860422039733 1102 1 0.11892250657420433 1102 2 0.14222100728185794 1102 3 0.16324190244251113 1102 4 0.15239738471635889 1102 5 0.19421228077026598 1102 6 0.23014895057353613 1102 7 0.25027077982982604 1102 8 0.23860680505922005 1102 9 0.28163404102274825 1102 10-11 0.3028172968810665 1102 12-13 0.3280085690772623 1102 14-15 0.360176993437868 1102 16-17 0.3094513347625565 1102 18-19 0.28774790644492043 1102 20-21 0.3193146281930055 1102 22-23 0.2920270895780206 1102 24-25 0.356783701437557 1102 26-27 0.3079240917085002 1102 28-29 0.25605233935968386 1102 30-31 0.36220346380614643 1102 32-33 0.4016368847351046 1102 34-35 0.26871254360505503 1102 36-37 0.1918925416061228 1102 38-39 0.19119390877047948 1102 40-41 0.21580709446007518 1102 42-43 0.23640510949749682 1102 44-45 0.3201887602827398 1102 46-47 0.17666650339040757 1102 48-49 0.1845647194857989 1102 50-51 0.2065107173506746 1102 52-53 0.31972767007648883 1102 54-55 0.22972924532557215 1102 56-57 0.19580323321595472 1102 58-59 0.24135094064211415 1102 60-61 0.22301181038213258 1102 62-63 0.14398785512776868 1102 64-65 0.16521917607680336 1102 66-67 0.2674525421817364 1102 68-69 0.2828374734186525 1102 70-71 0.24176794832838056 1102 72-73 0.11896305497807091 1102 74-75 0.09529291130034068 1102 76-77 0.19974108016038983 1102 78-79 0.17139169191027293 1102 80-81 0.1343020633789962 1102 82-83 0.09292788812944153 1102 84-85 0.13738757870527962 1102 86-87 0.15934817274312962 1102 88-89 0.10267658103577659 1102 90-91 0.11624694952784154 1102 92-93 0.09757049476958457 1102 94-95 0.1083294055503643 1102 96-97 0.12199374847379474 1102 98-99 0.1013033227388398 1102 100-101 0.04676185998149407 1103 1 0.2517094656649981 1103 2 0.20972433508374166 1103 3 0.27674177252855614 1103 4 0.18023571982972442 1103 5 0.1737942789048219 1103 6 0.22386578371630605 1103 7 0.23321731081301778 1103 8 0.20694711786716624 1103 9 0.2301238360796347 1103 10-11 0.19436401792896163 1103 12-13 0.259633580042113 1103 14-15 0.3394934180009912 1103 16-17 0.31290014942695166 1103 18-19 0.28046436153325516 1103 20-21 0.2987832255516949 1103 22-23 0.32184449779587254 1103 24-25 0.3580349650007051 1103 26-27 0.3532834520557202 1103 28-29 0.4456630236767154 1103 30-31 0.4810535122692414 1103 32-33 0.4695164657006572 1103 34-35 0.505358759830628 1103 36-37 0.4844799105713946 1103 38-39 0.49647327415446796 1103 40-41 0.41140930655117103 1103 42-43 0.40177780235641336 1103 44-45 0.3988148357810317 1103 46-47 0.376554462693818 1103 48-49 0.3242530062727198 1103 50-51 0.32631247076025716 1103 52-53 0.36821272754175993 1103 54-55 0.32947703111443616 1103 56-57 0.32872984779136516 1103 58-59 0.292927496692883 1103 60-61 0.21509580855656907 1103 62-63 0.35412793289835776 1103 64-65 0.3904343098322727 1103 66-67 0.3220421745388933 1103 68-69 0.31495548940573315 1103 70-71 0.3871960024836838 1103 72-73 0.3125924749048643 1103 74-75 0.29328946279966317 1103 76-77 0.30686428774508556 1103 78-79 0.27430459331579726 1103 80-81 0.3017013046481978 1103 82-83 0.32155120152942374 1103 84-85 0.32763157678258636 1103 86-87 0.26106890713483466 1103 88-89 0.3186905494848098 1103 90-91 0.35549051112025154 1103 92-93 0.3928606281697675 1103 94-95 0.40633784250051974 1103 96-97 0.43224615080337614 1103 98-99 0.4185622770644244 1103 100-101 0.3383182927868873 1104 1 0.11766353858024203 1104 2 0.12595163810040333 1104 3 0.19111634081428264 1104 4 0.07906894287037858 1104 5 0.07295345904985595 1104 6 0.06440748286167519 1104 7 0.06886581340022957 1104 8 0.062344292499496134 1104 9 0.10435282960980885 1104 10-11 0.11687405090776082 1104 12-13 0.10437109977598169 1104 14-15 0.10939771232737883 1104 16-17 0.11327847009630432 1104 18-19 0.16006203779969752 1104 20-21 0.1032993350495488 1104 22-23 0.14262323439509572 1104 24-25 0.10503085156209835 1104 26-27 0.08492102569147164 1104 28-29 -0.013469810091010004 1104 30-31 0.012705876802435512 1104 32-33 0.0473054990632491 1104 34-35 0.1671764275076839 1104 36-37 0.019641035414977637 1104 38-39 0.08564944159658694 1104 40-41 -0.04334215506468553 1104 42-43 0.023760923193549388 1104 44-45 0.006751449260612219 1104 46-47 -0.09975830876105363 1104 48-49 0.11037041039278961 1104 50-51 0.009004730940517902 1104 52-53 -0.028650597297897207 1104 54-55 0.08059036802612951 1104 56-57 0.07045397253167351 1104 58-59 0.03145473847344249 1104 60-61 -0.10091877972458008 1104 62-63 0.06440314190908936 1104 64-65 0.1414743913608234 1104 66-67 0.00892081701967129 1104 68-69 0.06483134101260646 1104 70-71 0.002880257480619264 1104 72-73 0.027160796002384302 1104 74-75 0.07210566112220818 1104 76-77 0.14410416325662823 1104 78-79 0.05211021625854073 1104 80-81 0.11501679723043523 1104 82-83 0.008653019808054552 1104 84-85 -0.007168294796734642 1104 86-87 -0.018308201574427585 1104 88-89 -0.004083021449257984 1104 90-91 -4.1543956656298064E-4 1104 92-93 -0.03543504748845905 1104 94-95 0.01843322634457678 1104 96-97 0.06278397003122294 1104 98-99 0.09857387193621747 1104 100-101 0.11298236786056393 1105 1 0.10075045192872523 1105 2 0.08442965744564646 1105 3 0.1463098787386521 1105 4 0.05398174752370721 1105 5 0.07319157343624738 1105 6 0.1402298684579435 1105 7 0.09939746089052903 1105 8 0.10154085853123007 1105 9 0.07783076880822648 1105 10-11 0.06649897789834114 1105 12-13 0.12616693174285132 1105 14-15 0.14295486345291408 1105 16-17 0.14130227849263122 1105 18-19 0.079226558031678 1105 20-21 0.060622359592066744 1105 22-23 0.14243072652826072 1105 24-25 0.14717623311479855 1105 26-27 0.22279149664466047 1105 28-29 0.21919363717000806 1105 30-31 0.30572478591263774 1105 32-33 0.20769621428689078 1105 34-35 0.19832954348257914 1105 36-37 0.22903159962278608 1105 38-39 0.14452272247380904 1105 40-41 0.134188039953699 1105 42-43 0.17882799521866133 1105 44-45 0.15789054868302088 1105 46-47 0.1550661753739675 1105 48-49 0.16760412490650367 1105 50-51 0.19615352092148441 1105 52-53 0.2090737050906597 1105 54-55 0.246581886106938 1105 56-57 0.16921780314535795 1105 58-59 0.18402157000877395 1105 60-61 0.18243421333399112 1105 62-63 0.1936266413124983 1105 64-65 0.21147704604715756 1105 66-67 0.19296908299433824 1105 68-69 0.1293802087365492 1105 70-71 0.0825490080228164 1105 72-73 0.08516770430918186 1105 74-75 0.14949598745351267 1105 76-77 0.11499610273796179 1105 78-79 0.07248044590330593 1105 80-81 0.1536074897412263 1105 82-83 0.14823776064174154 1105 84-85 0.13957666392943935 1105 86-87 0.1506267934059693 1105 88-89 0.0828872475057274 1105 90-91 0.11956759607728884 1105 92-93 0.11860509589089574 1105 94-95 0.08164242241996433 1105 96-97 0.02254096177211906 1105 98-99 0.11442274595832558 1105 100-101 -0.003585012421016387 1106 1 0.011583022057006787 1106 2 0.030601234472872108 1106 3 0.02534842635654755 1106 4 -6.273407395553932E-4 1106 5 0.05057347653042399 1106 6 0.021814312841932804 1106 7 0.07781861293676684 1106 8 0.11071398589145076 1106 9 0.10794832733434845 1106 10-11 0.06028783757465561 1106 12-13 0.06494335674356932 1106 14-15 0.010846568628139153 1106 16-17 0.09577925983492719 1106 18-19 0.08712924540893141 1106 20-21 0.028691536332850376 1106 22-23 0.003081993573253783 1106 24-25 0.0012527356555054325 1106 26-27 0.04299042324823432 1106 28-29 -0.04816151311761985 1106 30-31 0.036663398571462835 1106 32-33 -0.00774203924351724 1106 34-35 0.047139917798141084 1106 36-37 0.06806231088735615 1106 38-39 -0.01771968581664396 1106 40-41 -0.009241417081341297 1106 42-43 -0.03195269948835033 1106 44-45 -0.06617973973876445 1106 46-47 -0.023755548616463784 1106 48-49 -0.008697139204315363 1106 50-51 -0.0017749431561924212 1106 52-53 -0.028012739309723145 1106 54-55 0.05149677133374553 1106 56-57 0.02221368749384567 1106 58-59 -0.022736912836272438 1106 60-61 -0.04306721257576385 1106 62-63 -0.027109974840122675 1106 64-65 -0.06235421481922998 1106 66-67 -0.06417282802374302 1106 68-69 -0.05294833277950062 1106 70-71 -0.0854222971527534 1106 72-73 -0.1302478909569409 1106 74-75 -0.06454078987456668 1106 76-77 -0.1008121422574888 1106 78-79 -0.07683885006816027 1106 80-81 -0.05752081840846657 1106 82-83 -0.19317671141307002 1106 84-85 -0.07497833489069805 1106 86-87 -0.06846409269096654 1106 88-89 -0.0649547477741379 1106 90-91 -0.026638848974009477 1106 92-93 0.025797829392956828 1106 94-95 -0.06507465734298279 1106 96-97 -0.03464768130417184 1106 98-99 -0.10161144489938323 1106 100-101 -0.13872436728954796 1107 1 -0.011516400800637427 1107 2 0.025051612337758655 1107 3 0.04794247628996473 1107 4 0.004283803264847563 1107 5 0.05532399063172022 1107 6 0.05669413682988278 1107 7 0.03317910408886604 1107 8 0.05639042928360283 1107 9 0.08522783614962037 1107 10-11 0.047886373820922756 1107 12-13 -0.09931213026099073 1107 14-15 -0.19597530780286831 1107 16-17 -0.2033247098468678 1107 18-19 0.05893639297332243 1107 20-21 0.08675915397277123 1107 22-23 0.005604997110573606 1107 24-25 -0.08965032334666745 1107 26-27 -0.20320011183255815 1107 28-29 -0.13761347134934 1107 30-31 -0.09964660813666626 1107 32-33 -0.06667137720788219 1107 34-35 0.07597246056518259 1107 36-37 -0.04317351681520165 1107 38-39 0.010544641057826709 1107 40-41 0.017407605900430667 1107 42-43 0.015470018883682712 1107 44-45 8.901762101700683E-4 1107 46-47 0.011288961078420812 1107 48-49 0.012694857482344446 1107 50-51 -3.4482684601044866E-4 1107 52-53 -0.03530763785735758 1107 54-55 -0.04925165320002378 1107 56-57 -0.04929604852090108 1107 58-59 -0.07132202495265716 1107 60-61 -0.06109522811148338 1107 62-63 -0.11123914432845794 1107 64-65 -0.029281585571354185 1107 66-67 -0.01343104818644747 1107 68-69 0.05543839394989902 1107 70-71 -0.036227738981089885 1107 72-73 -0.1240840264781049 1107 74-75 -0.0564118148470385 1107 76-77 0.0071831222554763485 1107 78-79 -0.042379700871556736 1107 80-81 -0.0930457425316682 1107 82-83 -0.029088190990872675 1107 84-85 -0.03161318238047528 1107 86-87 -0.01079434779206423 1107 88-89 -0.030223569011919693 1107 90-91 -0.027910658816608702 1107 92-93 -0.05455176891565472 1107 94-95 -0.006974068229190777 1107 96-97 -0.039217268402776284 1107 98-99 -0.07413835132487279 1107 100-101 -0.18366169874240157 1108 1 -0.08939963023679454 1108 2 -0.0265922918885515 1108 3 -0.028094508666303142 1108 4 -0.06380355896041578 1108 5 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-1.4144783619154673 2216 84-85 -1.3887738504693878 2216 86-87 -1.3564928726824128 2216 88-89 -1.3931765457557752 2216 90-91 -1.455108125467973 2216 92-93 -1.6136237160865399 2216 94-95 -1.6889210262549241 2216 96-97 -1.69025747558808 2216 98-99 -1.6444043974927247 2216 100-101 -1.5221785555744667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 357820.0 3 97064.0 4 96150.0 5 102584.0 6 125281.0 7 139161.0 8 157269.0 9 184912.0 10 184248.0 11 177506.0 12 185796.0 13 200575.0 14 215491.0 15 228931.0 16 244530.0 17 245024.0 18 231159.0 19 234871.0 20 248345.0 21 273117.0 22 313362.0 23 364400.0 24 429574.0 25 508041.0 26 596640.0 27 681208.0 28 799958.0 29 940306.0 30 1108510.0 31 1329913.0 32 1626419.0 33 2019470.0 34 2560797.0 35 3389563.0 36 4707273.0 37 7082523.0 38 9188427.0 39 3078224.0 40 7246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.132078925416323 15.77590068392429 23.48456454222728 34.6074558484321 2 18.814101776111826 22.692848813727398 44.280180383635646 14.212869026525132 3 20.34294316518026 26.699091097747797 35.33292038074384 17.625045356328105 4 20.09852316802945 34.35946785814934 28.274481655585376 17.267527318235835 5 21.22084442000892 35.827707790855456 27.068354156347535 15.883093632788087 6 17.314309488243094 36.44718479905418 27.96671681927953 18.2717888934232 7 15.41285579272323 17.243858014727635 47.55897169125019 19.78431450129894 8 18.397252445585046 23.53995111858914 34.032940766796 24.029855669029814 9 19.76444202571167 22.66031271715426 34.040686356956215 23.534558900177863 10-11 21.799767666631055 34.04952351471094 24.839772691267758 19.310936127390242 12-13 19.45700231352684 27.248787898315157 32.89560388701885 20.398605901139145 14-15 19.94791672672562 28.01750015357366 32.351303445998084 19.683279673702632 16-17 20.985423141790633 28.00855025502647 30.32395758680676 20.68206901637614 18-19 21.70253236485329 28.612358667931193 29.071688416357254 20.61342055085826 20-21 20.860924735308945 29.300342015646752 29.188741187665673 20.64999206137863 22-23 20.855288744942634 29.497965836541923 30.152304733110164 19.49444068540528 24-25 21.0153709694318 29.233962553217367 29.938274334811858 19.81239214253897 26-27 20.951647875472577 29.70662671613456 28.889246443165383 20.452478965227474 28-29 21.350082460672976 29.298375541427774 28.393980045003143 20.957561952896107 30-31 20.60414940276049 29.16533419435794 29.567032319913768 20.66348408296781 32-33 21.170595557937318 29.283915660925015 29.15068724046555 20.394801540672113 34-35 20.57326230204141 30.169814851375047 28.411909077766406 20.84501376881713 36-37 21.01429292006976 29.14170460136834 28.774685403757232 21.069317074804665 38-39 21.285914143603392 28.553756975331247 29.38418025439474 20.776148626670622 40-41 21.689801741096264 29.003496908129094 28.604600329290307 20.702101021484335 42-43 21.59378241056992 29.76971007714772 28.450766500356554 20.185741011925803 44-45 21.28278520634266 29.464402170049294 28.599721024370567 20.65309159923748 46-47 21.007740820552502 29.149475914362178 28.67397108505386 21.168812180031455 48-49 21.44856260171227 28.85071626942972 28.57299946696869 21.12772166188932 50-51 21.046819156760453 29.2457824504193 28.78748706720007 20.91991132562018 52-53 20.8643897256369 29.46470773124188 29.056844411517503 20.61405813160372 54-55 20.751381380246375 29.539060231528413 28.919163215044634 20.790395173180578 56-57 21.330315522247233 29.059748539626177 28.310433301516223 21.29950263661036 58-59 21.000521667132034 29.327301741608284 28.183578153336864 21.488598437922814 60-61 21.372397591371314 29.34207342878974 28.377291059492983 20.90823792034596 62-63 20.135560433367782 30.858633899304245 27.939228799735467 21.066576867592506 64-65 20.368398869970665 30.411364253107443 28.083537183834455 21.136699693087433 66-67 20.964931770886306 29.071850535231462 28.723382272377844 21.239835421504385 68-69 21.482343743164588 29.742067556032158 27.753940843323083 21.021647857480172 70-71 20.864684609230107 30.534319865800047 27.530435227632676 21.07056029733717 72-73 20.746630312752828 30.22568824641808 28.100552969638432 20.927128471190663 74-75 20.941962605622965 29.54134536966001 28.271882603272854 21.24480942144417 76-77 21.57405230962396 29.531512760176586 27.780341745271325 21.11409318492813 78-79 21.235172526394436 30.424732267172704 27.23967687920797 21.10041832722489 80-81 21.065503614641703 30.464117254144092 27.931217019831404 20.5391621113828 82-83 20.895456302502495 30.252167137077308 27.908555538697954 20.943821021722243 84-85 21.127299308525913 30.51717055566731 27.528009017482724 20.827521118324054 86-87 21.6098804967744 30.634741103511743 26.8459059249706 20.909472474743254 88-89 21.23044588014676 30.725968082531946 27.272024514613065 20.771561522708232 90-91 21.48112642394688 30.600367005015393 27.29687293411341 20.62163363692432 92-93 21.357645253879003 30.750241314524345 27.197022365730945 20.69509106586571 94-95 21.513334874400627 30.926089269174064 26.875455311944325 20.685120544480988 96-97 21.65226875554432 30.289923889350618 27.31978194195844 20.73802541314662 98-99 22.279019100218513 30.58893004671028 26.725044516902273 20.407006336168934 100-101 22.51639145390116 31.289529406053795 26.026512925351142 20.1675662146939 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 99545.0 1 73366.5 2 46639.5 3 44445.0 4 39688.0 5 34032.0 6 29718.0 7 26747.0 8 25558.5 9 25054.5 10 27897.5 11 30663.0 12 29099.0 13 29403.0 14 31064.0 15 45580.0 16 87662.0 17 95498.5 18 88104.5 19 92627.0 20 93567.5 21 103094.0 22 110600.0 23 137749.5 24 185251.5 25 228760.5 26 268164.0 27 331347.0 28 420204.5 29 512457.0 30 636863.5 31 790106.0 32 964508.0 33 1195795.0 34 1479555.5 35 1813807.5 36 2136635.5 37 2239594.5 38 2095487.5 39 1876816.0 40 1709737.5 41 1629478.0 42 1586318.0 43 1567000.5 44 1534509.0 45 1504252.0 46 1485846.5 47 1458042.5 48 1435679.5 49 1396535.0 50 1334930.0 51 1276828.0 52 1195215.5 53 1094822.0 54 997096.0 55 895463.0 56 789418.5 57 670517.0 58 559299.0 59 459408.5 60 359972.0 61 277554.0 62 216681.5 63 161945.0 64 124127.0 65 97286.0 66 68373.0 67 48533.5 68 39034.5 69 32295.5 70 23836.5 71 15043.5 72 10172.5 73 7707.0 74 6020.0 75 4392.0 76 3052.5 77 2324.0 78 1740.0 79 1289.5 80 1007.0 81 828.5 82 695.0 83 638.0 84 563.0 85 437.0 86 382.0 87 316.0 88 224.0 89 151.5 90 103.5 91 75.5 92 61.5 93 61.5 94 59.0 95 56.0 96 70.5 97 78.0 98 93.5 99 133.5 100 485.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002335334929570956 2 0.001956934543092057 3 0.13280286226530444 4 0.3443242001959263 5 0.37860190147761547 6 0.3780085517591722 7 0.4015253521093963 8 0.20733206501285847 9 0.14216883159454252 10-11 0.18214828781214001 12-13 1.3434333247771558E-5 14-15 0.0015863708510076915 16-17 0.04839942458063833 18-19 0.0031290801189601254 20-21 6.291746071039679E-4 22-23 0.002667834677453302 24-25 0.0058909551291478285 26-27 0.014471015963391263 28-29 0.026687302996698203 30-31 0.03158411746551093 32-33 0.02531252289434291 34-35 0.026606696997211566 36-37 0.031399395383354074 38-39 0.026303304971366064 40-41 0.018773361185990104 42-43 0.011537853204294473 44-45 0.004193751028846021 46-47 0.002346530207277432 48-49 0.002428255734534709 50-51 0.0011990142423636116 52-53 0.001176623686950659 54-55 0.0012538711031253452 56-57 3.9855188635055624E-4 58-59 1.4889719349613474E-4 60-61 2.1271027642304966E-5 62-63 0.0011195277706476298 64-65 0.11733434705826615 66-67 0.2045265284196155 68-69 0.19443510509499776 70-71 0.0299708779480077 72-73 3.0227249807486007E-5 74-75 0.0 76-77 4.478111082590519E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.4553861018419187E-5 88-89 0.0 90-91 3.873566086440799E-4 92-93 1.2986522139512506E-4 94-95 0.0 96-97 2.2390555412952594E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.4661688E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95688667795058 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24625977841215 42.21302712314294 2 10.149730633750648 10.546968086990118 3 3.229500913517879 5.033844389699588 4 1.6165809630781618 3.35970055617537 5 0.8651183786779305 2.247442878199078 6 0.5801663059105445 1.808618100633562 7 0.3872225293140387 1.408321395230323 8 0.27666763219906026 1.1499831050898786 9 0.2124624308020976 0.9934997796455849 >10 1.242739215316911 12.142703525196621 >50 0.11146404494888425 4.017465336573906 >100 0.07283113887934749 7.211732354910408 >500 0.005620595798048667 1.9937997349702319 >1k 0.0032562496134636134 3.220982333829591 >5k 2.2837572680248664E-4 0.809790804170763 >10k+ 1.508140540812372E-4 1.8421204955420527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109587 0.2453713796039236 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 75787 0.16969130230814383 No Hit CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 68673 0.15376266118736936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.515651625169205E-4 2.2390555412952594E-6 0.0 8.956222165181038E-6 0.0 2 6.784338290124637E-4 6.717166623885779E-6 0.0 2.2390555412952596E-5 2.2390555412952594E-6 3 7.366492730861404E-4 6.717166623885779E-6 0.0 2.4629610954247856E-5 4.478111082590519E-6 4 9.605548272156664E-4 8.956222165181038E-6 0.0 4.030299974331467E-5 4.478111082590519E-6 5 0.0010299655489958194 8.956222165181038E-6 0.0 4.478111082590519E-5 4.478111082590519E-6 6 0.001128483992812811 1.1195277706476298E-5 0.0 5.149827744979097E-5 4.478111082590519E-6 7 0.0011822213258038971 1.1195277706476298E-5 0.0 5.597638853238149E-5 4.478111082590519E-6 8 0.0012896959917860695 1.1195277706476298E-5 0.0 6.269355515626727E-5 6.717166623885779E-6 9 0.0014531470463006234 1.1195277706476298E-5 0.0 6.941072178015305E-5 6.717166623885779E-6 10-11 0.0019882813206701907 1.1195277706476298E-5 0.0 7.053024955080067E-5 8.956222165181038E-6 12-13 0.0028044170654723125 1.1195277706476298E-5 0.0 8.508411056921986E-5 8.956222165181038E-6 14-15 0.003724668892944664 1.1195277706476298E-5 0.0 1.0635513821152483E-4 8.956222165181038E-6 16-17 0.00487890202448237 1.3434333247771558E-5 0.0 1.2426758254188692E-4 8.956222165181038E-6 18-19 0.006304060876516804 1.3434333247771558E-5 0.0 1.354628602483632E-4 1.1195277706476298E-5 20-21 0.007904985588542914 1.3434333247771558E-5 0.0 1.432995546428966E-4 1.1195277706476298E-5 22-23 0.009471204939678948 1.3434333247771558E-5 0.0 1.5225577680807767E-4 1.1195277706476298E-5 24-25 0.011112432651448373 1.567338878906682E-5 0.0 1.690486933677921E-4 1.1195277706476298E-5 26-27 0.012698803502456064 1.9031972101009706E-5 0.0 1.836025543862113E-4 1.3434333247771558E-5 28-29 0.014374736575115565 2.1271027642304966E-5 0.0 1.9031972101009705E-4 1.3434333247771558E-5 30-31 0.015926402065233183 2.4629610954247856E-5 0.0 1.9815641540463046E-4 1.679291655971445E-5 32-33 0.017628084276617578 2.6868666495543117E-5 0.0 2.0375405425786863E-4 2.2390555412952596E-5 34-35 0.01935663515449752 2.7988194266190743E-5 0.0 2.138298041936973E-4 2.2390555412952596E-5 36-37 0.021256473781286547 3.2466305348781264E-5 0.0 2.2838366521211648E-4 2.2390555412952596E-5 38-39 0.02344962868398525 3.6944416431371785E-5 0.0 2.440570540011833E-4 2.3510083183600226E-5 40-41 0.025886840640685145 4.366158305525756E-5 0.0 2.608499705608978E-4 2.6868666495543117E-5 42-43 0.028676703845139036 4.478111082590519E-5 0.0 2.675671371847835E-4 2.7988194266190743E-5 44-45 0.03196587643530177 4.5900638596552826E-5 0.0 2.765233593499646E-4 2.9107722036838374E-5 46-47 0.03588534316033913 4.702016636720045E-5 0.0 2.776428871206122E-4 2.9107722036838374E-5 48-49 0.04057504499158204 5.0378749679143346E-5 0.0 2.84360053744498E-4 2.9107722036838374E-5 50-51 0.046536530370280674 5.485686076173386E-5 0.0 2.9107722036838374E-4 2.9107722036838374E-5 52-53 0.0541761878771801 5.597638853238149E-5 0.0 3.000334425335648E-4 2.9107722036838374E-5 54-55 0.06304508687625063 5.597638853238149E-5 0.0 3.033920258455077E-4 2.9107722036838374E-5 56-57 0.07457622291392121 5.597638853238149E-5 0.0 3.2354352571716503E-4 2.9107722036838374E-5 58-59 0.0895319944020029 5.709591630302912E-5 0.0 3.4481455335947E-4 2.9107722036838374E-5 60-61 0.10930845247049328 5.821544407367675E-5 0.0 3.593684143778892E-4 3.134677757813364E-5 62-63 0.13457955283732223 5.821544407367675E-5 0.0 3.7392227539630835E-4 3.35858331194289E-5 64-65 0.1676246540435283 6.045449961497201E-5 0.0 3.873566086440799E-4 3.6944416431371785E-5 66-67 0.20977487460841157 6.157402738561964E-5 2.2390555412952594E-6 3.8959566418537517E-4 3.806394420201941E-5 68-69 0.2636980492094253 6.269355515626727E-5 2.2390555412952594E-6 3.940737752679657E-4 3.806394420201941E-5 70-71 0.3344309332867132 6.269355515626727E-5 2.2390555412952594E-6 4.0638858074508964E-4 3.806394420201941E-5 72-73 0.4256881199832841 6.269355515626727E-5 2.2390555412952594E-6 4.2318149730480407E-4 3.806394420201941E-5 74-75 0.5410498591096691 6.38130829269149E-5 2.2390555412952594E-6 4.3437677501128035E-4 3.806394420201941E-5 76-77 0.6836709799235532 6.829119400950542E-5 2.2390555412952594E-6 4.455720527177567E-4 3.806394420201941E-5 78-79 0.8598992048845087 6.941072178015305E-5 2.2390555412952594E-6 4.578868581948806E-4 4.030299974331467E-5 80-81 1.0776518343865553 6.941072178015305E-5 2.2390555412952594E-6 4.634844970481187E-4 4.030299974331467E-5 82-83 1.348131982830564 6.941072178015305E-5 2.2390555412952594E-6 4.713211914426521E-4 4.030299974331467E-5 84-85 1.6887650999666648 6.941072178015305E-5 2.2390555412952594E-6 4.8139694137848085E-4 4.030299974331467E-5 86-87 2.0996709752663176 7.16497773214483E-5 2.2390555412952594E-6 4.914726913143095E-4 4.030299974331467E-5 88-89 2.5676223881193208 7.16497773214483E-5 2.2390555412952594E-6 5.037874967914334E-4 4.030299974331467E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTAT 77800 0.0 19.284939 1 GATCGGG 5260 0.0 18.388042 1 CGCCGGA 3680 0.0 18.18283 46-47 GCGAACT 3090 0.0 18.040667 1 GGAAAAC 148065 0.0 17.83683 1 CGGCGAG 4045 0.0 17.668428 1 ATCGGGA 5410 0.0 17.525928 2 CGACGGC 3950 0.0 16.525274 1 ATCCGGT 6430 0.0 16.525023 6 TCCGGTA 6040 0.0 16.407257 7 TATTATA 47120 0.0 16.248854 9 GCCGGAT 4855 0.0 16.177036 46-47 CTTTATT 115585 0.0 15.84142 1 CGGGAGT 6260 0.0 15.831213 1 CGCAGTT 5970 0.0 15.722338 1 CCGGTAA 6260 0.0 15.664393 8 TTATATA 52380 0.0 15.281951 4 GAGCGGC 9320 0.0 14.755498 9 GGCGCCG 5640 0.0 14.683091 44-45 CTGGAAT 80945 0.0 14.597782 1 >>END_MODULE