FastQCFastQC Report
Fri 27 May 2016
SRR1233106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44661688
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1161920.2601603414541788No Hit
GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT745550.1669327858812681No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG712180.1594610575399658No Hit
TTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTT655200.1467029190656654No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC651520.14587894662646875No Hit
CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT602480.1348986182519568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520180.11647119114709681No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC493530.11050410812954495TruSeq Adapter, Index 8 (100% over 50bp)
CGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488490.10937562413673213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGC90850.044.5105631
GACGGCC119650.035.8436782
ACGGCCA136650.031.3089373
CGGCCAG235000.029.6375521
TATGCCG116200.024.89787146-47
TGCCGTC127500.022.67829548-49
CGTATGC130600.022.42522844-45
ATGCCGT126750.022.2263146-47
CTCGTAT122700.021.95415142-43
TCTCGTA117150.021.75850540-41
TCGTATG136050.021.14313542-43
GCCGTCT141150.020.06470948-49
CGGCGCT47950.019.5454981
CCGTCTT151000.019.19522750-51
CGGCGAG38000.018.904381
AATCTCG137550.018.77307138-39
CTTTTAT754150.018.3697281
CGTCTTC158750.018.00309450-51
GCGAACT26400.017.4798181
CCGACGG22550.017.2996141
Produced by FastQC (version 0.11.5)