##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44661688 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13641490666452 33.0 31.0 34.0 30.0 34.0 2 32.43855574827355 34.0 31.0 34.0 31.0 34.0 3 32.545162556327924 34.0 31.0 34.0 31.0 34.0 4 35.42991662115413 37.0 35.0 37.0 33.0 37.0 5 35.861045355025546 37.0 35.0 37.0 35.0 37.0 6 35.865504232621035 37.0 35.0 37.0 35.0 37.0 7 35.863702442236395 37.0 35.0 37.0 35.0 37.0 8 35.90424504331319 37.0 35.0 37.0 35.0 37.0 9 37.67332258019446 39.0 37.0 39.0 35.0 39.0 10-11 37.70490575725664 39.0 37.0 39.0 35.0 39.0 12-13 37.61839834625149 39.0 37.0 39.0 35.0 39.0 14-15 38.90004936669658 40.0 38.0 41.0 35.0 41.0 16-17 38.81512741972493 40.0 38.0 41.0 35.0 41.0 18-19 38.7833043278615 40.0 38.0 41.0 34.0 41.0 20-21 38.83926896135229 40.0 38.0 41.0 35.0 41.0 22-23 38.79610236630555 40.0 38.0 41.0 35.0 41.0 24-25 38.16204329312407 40.0 37.5 41.0 32.5 41.0 26-27 37.08826772288589 40.0 36.0 41.0 30.5 41.0 28-29 36.68727250076172 39.0 36.0 41.0 29.5 41.0 30-31 36.5784780906624 39.0 36.0 41.0 30.0 41.0 32-33 36.491725603832975 39.0 35.5 41.0 29.5 41.0 34-35 36.40589473465491 39.0 35.0 41.0 29.5 41.0 36-37 36.2865088641522 39.0 35.0 41.0 29.0 41.0 38-39 36.159762770274156 39.0 35.0 41.0 29.0 41.0 40-41 36.015716893638235 39.0 35.0 41.0 27.5 41.0 42-43 35.86728325181082 39.0 35.0 41.0 27.0 41.0 44-45 35.724540886139366 38.5 35.0 40.5 27.0 41.0 46-47 35.8875229704708 39.0 35.0 41.0 27.0 41.0 48-49 35.8162690357785 39.0 35.0 41.0 27.0 41.0 50-51 35.71130907546531 39.0 35.0 41.0 27.0 41.0 52-53 35.561336553154916 39.0 34.5 41.0 26.5 41.0 54-55 35.4074801539073 39.0 34.0 41.0 26.0 41.0 56-57 35.04506105994024 38.0 34.0 41.0 25.5 41.0 58-59 34.8709833918503 38.0 34.0 41.0 25.0 41.0 60-61 34.597236819620434 38.0 33.5 40.0 24.0 41.0 62-63 34.45739648264078 38.0 33.0 40.0 24.0 41.0 64-65 34.27138877957322 37.0 33.0 40.0 24.0 41.0 66-67 33.98407250079755 37.0 33.0 40.0 23.0 41.0 68-69 33.64070213826221 36.5 32.5 39.5 23.0 41.0 70-71 33.30160653578521 36.0 32.0 39.0 22.5 41.0 72-73 32.95831161822635 35.5 32.0 39.0 22.0 41.0 74-75 32.5645045368639 35.0 32.0 38.5 22.0 40.0 76-77 32.20709706493852 35.0 31.0 37.0 21.0 39.5 78-79 31.865626932864693 35.0 31.0 37.0 20.5 39.0 80-81 31.52966604173134 35.0 31.0 36.5 20.0 39.0 82-83 31.192131755073834 35.0 31.0 36.0 19.5 37.5 84-85 30.887543928030667 35.0 31.0 36.0 18.5 37.0 86-87 30.59620966408614 35.0 31.0 35.0 18.0 37.0 88-89 30.328859155524977 34.5 31.0 35.0 15.0 36.0 90-91 30.07959803265833 34.0 31.0 35.0 10.5 36.0 92-93 29.84422555860405 34.0 30.0 35.0 7.0 36.0 94-95 29.6019725564336 34.0 30.0 35.0 2.0 36.0 96-97 29.325044163131498 34.0 30.0 35.0 2.0 35.0 98-99 29.029069232672086 34.0 30.0 35.0 2.0 35.0 100-101 28.022069116599443 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.363846297484244 1101 2 0.28039473179183005 1101 3 0.2629018951480617 1101 4 0.8051452326957076 1101 5 0.3866123268338839 1101 6 0.3064019495004331 1101 7 0.1414085613383662 1101 8 0.19821238891720583 1101 9 0.25963173519136973 1101 10-11 0.24953036255085692 1101 12-13 0.27621403853906656 1101 14-15 0.3004343415097992 1101 16-17 0.2699939118968402 1101 18-19 0.29262683144550294 1101 20-21 0.34575211306542286 1101 22-23 0.32005003688662015 1101 24-25 0.3334310896372301 1101 26-27 0.3791319541966516 1101 28-29 0.5378546798022654 1101 30-31 0.5080810748314306 1101 32-33 0.45168372404497603 1101 34-35 0.4838986325478487 1101 36-37 0.45995383439780824 1101 38-39 0.38481493893992536 1101 40-41 0.16421535273052967 1101 42-43 0.28128616890543157 1101 44-45 0.4123370343137438 1101 46-47 0.4432378240835888 1101 48-49 0.5173295992775664 1101 50-51 0.4848327269881949 1101 52-53 0.4717673741125168 1101 54-55 0.3408383677916902 1101 56-57 0.45177443266464223 1101 58-59 0.4611957185061115 1101 60-61 0.5706121736219956 1101 62-63 0.4079331156398922 1101 64-65 0.35523424845636953 1101 66-67 0.40883771280321923 1101 68-69 0.3909376589634874 1101 70-71 0.46784288890241044 1101 72-73 0.4645202028750077 1101 74-75 0.3692829536446425 1101 76-77 0.39541793502760925 1101 78-79 0.41058606838990386 1101 80-81 0.36363883006285036 1101 82-83 0.36309061913582497 1101 84-85 0.4086658464267785 1101 86-87 0.3200931331738097 1101 88-89 0.3594926836967183 1101 90-91 0.35962445993259706 1101 92-93 0.34638651656815966 1101 94-95 0.3736854233034599 1101 96-97 0.417248496077093 1101 98-99 0.4328822487127155 1101 100-101 0.30158218560504935 1102 1 0.31051645799592364 1102 2 0.2344912125113936 1102 3 0.20957373193863305 1102 4 0.7823742787514405 1102 5 0.3220676934857849 1102 6 0.25520720010078435 1102 7 0.13006708907855113 1102 8 0.13027270798923496 1102 9 0.11061811533564736 1102 10-11 0.16466125089115735 1102 12-13 0.15568572423605787 1102 14-15 0.155356017294352 1102 16-17 0.2071244143358797 1102 18-19 0.2300934678870945 1102 20-21 0.28284131720818806 1102 22-23 0.25949941301062296 1102 24-25 0.29298684629604566 1102 26-27 0.17845071327106155 1102 28-29 0.3559262098584952 1102 30-31 0.36690804234451235 1102 32-33 0.23709037477540562 1102 34-35 0.25188524226990694 1102 36-37 0.15134847762732306 1102 38-39 0.1998655539965597 1102 40-41 0.23098447535468125 1102 42-43 0.20593472258487822 1102 44-45 0.16095239412819495 1102 46-47 0.2405225595905165 1102 48-49 0.2746774065451447 1102 50-51 0.2605787368362158 1102 52-53 0.26134499686833834 1102 54-55 0.26912885532345854 1102 56-57 0.29151264505633634 1102 58-59 0.3424552987844507 1102 60-61 0.4434974511759364 1102 62-63 0.42092736825306787 1102 64-65 0.3670086008360727 1102 66-67 0.32839158131606894 1102 68-69 0.3118656225922436 1102 70-71 0.25266667741999527 1102 72-73 0.3470009878552034 1102 74-75 0.27893151459176124 1102 76-77 0.2112483117507864 1102 78-79 0.28251890933110246 1102 80-81 0.3073701207719459 1102 82-83 0.29500177683107154 1102 84-85 0.3036742735704365 1102 86-87 0.25112922147366845 1102 88-89 0.22474930552593264 1102 90-91 0.24526500431642972 1102 92-93 0.25713167730120645 1102 94-95 0.27339409033679374 1102 96-97 0.25588676796834875 1102 98-99 0.2620068323276037 1102 100-101 0.20227449320545077 1103 1 0.2992614321618632 1103 2 0.23805032770543733 1103 3 0.20621325757792164 1103 4 0.7671973986648624 1103 5 0.3610150027965773 1103 6 0.24216217072077484 1103 7 0.10381910807267047 1103 8 0.12497870374713216 1103 9 0.1716797661642886 1103 10-11 0.2155323326778955 1103 12-13 0.2283680030943387 1103 14-15 0.2569469763420642 1103 16-17 0.25256244655074056 1103 18-19 0.34758380500661445 1103 20-21 0.31269817579175196 1103 22-23 0.28342221470123974 1103 24-25 0.29159258922440756 1103 26-27 0.2809908036063007 1103 28-29 0.4444046133467836 1103 30-31 0.3692206276894794 1103 32-33 0.315203829731594 1103 34-35 0.26773795463872574 1103 36-37 0.2198751327041748 1103 38-39 0.2934185647095262 1103 40-41 0.2847268511122749 1103 42-43 0.12736714440057284 1103 44-45 -0.03317754508493209 1103 46-47 0.22881605304699804 1103 48-49 0.17859026366564024 1103 50-51 0.22393488327815447 1103 52-53 0.2787568618801828 1103 54-55 0.32203849295770226 1103 56-57 0.2809788345006794 1103 58-59 0.3266172038358732 1103 60-61 0.480714251940384 1103 62-63 0.2896413729114258 1103 64-65 0.24997648821921814 1103 66-67 0.3281720557159815 1103 68-69 0.2352153162701356 1103 70-71 0.22481507043602278 1103 72-73 0.27326944211873183 1103 74-75 0.2949523451211391 1103 76-77 0.17696343692173855 1103 78-79 0.2539534842490738 1103 80-81 0.285550284148524 1103 82-83 0.25741615663019246 1103 84-85 0.2615378150493832 1103 86-87 0.3142928240846068 1103 88-89 0.3526530554779512 1103 90-91 0.32401396740641175 1103 92-93 0.3250377122006469 1103 94-95 0.30674789205485453 1103 96-97 0.21268701636171272 1103 98-99 0.24400087104617185 1103 100-101 0.15969723391311774 1104 1 0.21136885484324353 1104 2 0.16743690874564265 1104 3 0.14737853229482312 1104 4 0.6877314902726042 1104 5 0.31591092648352515 1104 6 0.18807251801320035 1104 7 0.08352549053938674 1104 8 0.0808456573813956 1104 9 0.08412921583985877 1104 10-11 0.10194222872007686 1104 12-13 0.09971542185874682 1104 14-15 0.1532667828536134 1104 16-17 0.17780091804532816 1104 18-19 0.14580403812916387 1104 20-21 0.06643273110958603 1104 22-23 0.14963912443554506 1104 24-25 -0.016884088993649016 1104 26-27 -0.12044307441226465 1104 28-29 0.07495685308565214 1104 30-31 -0.04203662877365133 1104 32-33 0.05176177736596088 1104 34-35 -0.014324463685305489 1104 36-37 0.06551557299412991 1104 38-39 -0.09006763518863892 1104 40-41 -0.1395034863497031 1104 42-43 -0.14626868038104845 1104 44-45 -0.011442514591301745 1104 46-47 -0.15065186145398002 1104 48-49 -0.07387000161279644 1104 50-51 0.07884593167138831 1104 52-53 0.09585275883978994 1104 54-55 0.13607644644478967 1104 56-57 0.10533002417654558 1104 58-59 0.1956140080719777 1104 60-61 0.25793093751168783 1104 62-63 0.15868613804995846 1104 64-65 -0.013836806788049216 1104 66-67 0.13838956601077257 1104 68-69 0.013570425764129368 1104 70-71 0.13586626052473605 1104 72-73 -0.030698091553276186 1104 74-75 0.09285123015720131 1104 76-77 0.09736496761622959 1104 78-79 -0.03623940955287708 1104 80-81 0.06363702418840589 1104 82-83 -0.038048539197600206 1104 84-85 -0.00417353114442065 1104 86-87 0.034170649448459045 1104 88-89 0.14497491054745026 1104 90-91 0.14514168992089793 1104 92-93 0.08811967525192443 1104 94-95 0.17768390918540078 1104 96-97 0.14438524400427255 1104 98-99 -0.005238313682770723 1104 100-101 0.050191600814432746 1105 1 0.22622550415174913 1105 2 0.17744542441145228 1105 3 0.1563505468287687 1105 4 0.7213676645029636 1105 5 0.2953801899470676 1105 6 0.14384522124289134 1105 7 0.0668730555245034 1105 8 0.08882673240556471 1105 9 0.13518546316301183 1105 10-11 0.10751232555692525 1105 12-13 0.13143571401104737 1105 14-15 0.12052133363090434 1105 16-17 0.18000803380326857 1105 18-19 0.18431058557893465 1105 20-21 0.1892264930492047 1105 22-23 0.15137947675290775 1105 24-25 0.14950343666742327 1105 26-27 0.14849540506280334 1105 28-29 0.2927456914023452 1105 30-31 0.25400259493724064 1105 32-33 0.1911643786509032 1105 34-35 0.15558460435308774 1105 36-37 0.11735753932921966 1105 38-39 0.11014595962188878 1105 40-41 0.05202051141284869 1105 42-43 -0.04965548363541927 1105 44-45 -0.02122004749823958 1105 46-47 0.12837863871769883 1105 48-49 -0.05627325178401321 1105 50-51 0.02483601239841704 1105 52-53 -0.0014247915225098495 1105 54-55 0.1659520606040843 1105 56-57 0.009206923953719581 1105 58-59 0.04784264232521451 1105 60-61 0.17536878297815406 1105 62-63 0.07405820031779342 1105 64-65 0.1326859871947761 1105 66-67 0.11362287600223908 1105 68-69 0.09413829875046531 1105 70-71 -0.06153663177818203 1105 72-73 0.016195538745378713 1105 74-75 0.08794051628211719 1105 76-77 0.07533170479767648 1105 78-79 0.09960730320944577 1105 80-81 0.11742688493500353 1105 82-83 0.11744147803988625 1105 84-85 0.09973169060746656 1105 86-87 0.09565232927878853 1105 88-89 0.1303590965842112 1105 90-91 0.03869804703178659 1105 92-93 0.07141011960278121 1105 94-95 0.07049949759345964 1105 96-97 0.0633270093964029 1105 98-99 0.002706279190348937 1105 100-101 -0.013318314846596735 1106 1 0.11364656624252234 1106 2 0.12185770524701667 1106 3 0.1060487596522961 1106 4 0.6058087115992876 1106 5 0.20958340204734327 1106 6 0.12089892085727882 1106 7 0.09205653942228054 1106 8 0.05913760502320997 1106 9 0.0376663692274235 1106 10-11 0.018389046469998505 1106 12-13 0.0636517463780919 1106 14-15 0.07925806385838996 1106 16-17 0.0729111846015087 1106 18-19 0.11004780097125888 1106 20-21 0.09818337139794409 1106 22-23 0.08803156462013817 1106 24-25 0.02939872351691264 1106 26-27 -0.007056878725954618 1106 28-29 0.1417456620626325 1106 30-31 0.07017124413876985 1106 32-33 0.046171805105792885 1106 34-35 -0.031055745813276303 1106 36-37 -0.023715984364848453 1106 38-39 -0.03418403924069224 1106 40-41 -0.0907722172820371 1106 42-43 -0.029639377909582265 1106 44-45 -0.023299895260421977 1106 46-47 0.03317285671817416 1106 48-49 -0.0021511418476052313 1106 50-51 0.020409958142884932 1106 52-53 0.004630570611809048 1106 54-55 0.016111266136562108 1106 56-57 -0.04148052496046972 1106 58-59 -0.006314213330796292 1106 60-61 0.09403031159867226 1106 62-63 0.07693906968476938 1106 64-65 0.08104971614431378 1106 66-67 0.0684467989372024 1106 68-69 0.05004519297047949 1106 70-71 -0.0699998642757862 1106 72-73 -0.01781605973589251 1106 74-75 0.014099607140217074 1106 76-77 0.058341976935764706 1106 78-79 0.07072521579686963 1106 80-81 0.045383466819718166 1106 82-83 0.042295196993705986 1106 84-85 0.06326496994725872 1106 86-87 0.10097722387064678 1106 88-89 0.06918183696462421 1106 90-91 0.06825405751588676 1106 92-93 0.0608816862368613 1106 94-95 0.06657380240788413 1106 96-97 0.0679794341042026 1106 98-99 0.06577065016096384 1106 100-101 -0.07070643924056341 1107 1 0.08020127140609645 1107 2 0.07584382567988968 1107 3 0.08888990981568412 1107 4 0.6470181127002235 1107 5 0.23058279572432383 1107 6 0.0671638290948593 1107 7 0.03014563784421398 1107 8 0.011571761946427728 1107 9 6.426066419891185E-4 1107 10-11 0.003983959654476621 1107 12-13 0.03550118729481966 1107 14-15 0.04341397532773783 1107 16-17 0.08660965854476643 1107 18-19 0.09463965807236008 1107 20-21 0.076942628291512 1107 22-23 0.04501263081833429 1107 24-25 0.016677917192495784 1107 26-27 -0.04738492650276527 1107 28-29 0.10538233292577814 1107 30-31 0.020142407555333364 1107 32-33 0.007709735678425034 1107 34-35 -0.10772160160494337 1107 36-37 -0.12010631527601845 1107 38-39 -0.08402558999873122 1107 40-41 -0.14555929775254128 1107 42-43 -0.13347817940693574 1107 44-45 -0.18446467233726338 1107 46-47 -0.14257377710237762 1107 48-49 -0.2100462705761288 1107 50-51 -0.1425380295908596 1107 52-53 -0.11299388901939267 1107 54-55 -0.09413529830352729 1107 56-57 -0.15163237999704648 1107 58-59 -0.09930263646481308 1107 60-61 -0.0014076696648714915 1107 62-63 -0.12463128807202395 1107 64-65 -0.024665557659403703 1107 66-67 -0.0645673832002629 1107 68-69 -0.04908642902726257 1107 70-71 -0.06034013965768281 1107 72-73 -0.06906505030097776 1107 74-75 -0.09377534294594625 1107 76-77 -0.10226777716516722 1107 78-79 -0.03886499009465183 1107 80-81 -0.06159553335531598 1107 82-83 -0.11695424462543969 1107 84-85 -0.0835530769005004 1107 86-87 -0.08428096390251838 1107 88-89 -0.051499068065684384 1107 90-91 -0.0765078499639813 1107 92-93 -0.16919576236276157 1107 94-95 -0.13723778681844223 1107 96-97 -0.07507910849358268 1107 98-99 -0.10101391042477204 1107 100-101 -0.20364056310749135 1108 1 0.06247271596943449 1108 2 0.022480886568139624 1108 3 0.004171343661390381 1108 4 0.5873115421139303 1108 5 0.19061958666993917 1108 6 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-1.017969833890266 2216 84-85 -0.9624381652366765 2216 86-87 -0.9309481486123659 2216 88-89 -0.9220668131760235 2216 90-91 -0.9697477675556314 2216 92-93 -0.9799987739026719 2216 94-95 -0.9966676681542346 2216 96-97 -1.0642732996458335 2216 98-99 -0.9969269204666098 2216 100-101 -0.7687541652914582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6739.0 3 2743.0 4 3653.0 5 6499.0 6 15315.0 7 38140.0 8 74220.0 9 108971.0 10 121111.0 11 126262.0 12 140453.0 13 159362.0 14 178768.0 15 195360.0 16 209569.0 17 218425.0 18 225412.0 19 234727.0 20 245942.0 21 262984.0 22 288099.0 23 327731.0 24 383558.0 25 455316.0 26 547581.0 27 657396.0 28 788512.0 29 927956.0 30 1109578.0 31 1332370.0 32 1612304.0 33 1984130.0 34 2505665.0 35 3316534.0 36 4747728.0 37 7456283.0 38 1.0130736E7 39 3504451.0 40 11105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.34487887796616 16.027408158546248 22.449441347397002 35.1782716160906 2 19.241622362066906 22.69933061219883 43.24262484365898 14.81642218207528 3 20.99962052486686 26.931516784587274 33.942174330714955 18.126688359830915 4 20.484095898927958 34.04703601888043 27.642139276061396 17.826728806130212 5 21.92137251955188 35.26713320822088 26.59959919114566 16.211895081081575 6 17.60672702650825 36.374644889639804 27.86739999786824 18.15122808598371 7 15.622565328515648 16.559941177507305 47.66409212127583 20.15340137270122 8 18.749119743500028 22.84601542337018 34.261654170625626 24.14321066250416 9 20.119291057695804 21.86770011917149 34.151031192551436 23.861977630581272 10-11 22.136504334542842 33.69096685284264 24.7829739440211 19.38955486859341 12-13 19.733924969427935 26.596034614723923 33.22991061152906 20.440129804319085 14-15 20.26115425820896 27.318353932345772 32.49436071471369 19.926131094731574 16-17 21.543400001464345 27.194158373257387 30.451424688136935 20.811016937141332 18-19 21.85221614959521 27.752774862678464 29.511453587977783 20.883555399748545 20-21 21.15891253371346 28.331813163891162 29.717780707258534 20.791493595136842 22-23 20.895639053818115 28.661766073528245 30.862686944030337 19.579907928623307 24-25 21.163771063049694 28.127979717217087 30.84326625583696 19.864982963896257 26-27 21.09280191434469 28.487007768712715 29.90253728793235 20.517653029010248 28-29 21.571058597225367 28.15219692434734 29.51753879719216 20.759205681235134 30-31 20.53384674060017 27.978139037255467 30.867547246019594 20.620466976124767 32-33 21.28138087112185 28.085804015916338 30.47066597666399 20.162149136297828 34-35 20.552249073570476 28.955149963633133 29.801237860605166 20.691363102191225 36-37 21.005792414730607 27.8183550973375 30.350287961589835 20.825564526342056 38-39 21.33528613317464 27.355699245209863 30.806153399582808 20.502861222032685 40-41 21.492419952298896 27.796578014046837 30.30154823659747 20.409453797056795 42-43 21.428321612049828 28.475328857621385 30.112637215682845 19.98371231464594 44-45 21.195749920426124 28.163171411257952 30.210185082156706 20.430893586159222 46-47 20.85461778523343 27.968101458853223 30.329220084840347 20.848060671073 48-49 21.469251210891464 27.57282732273452 30.21286475711288 20.745056709261142 50-51 20.944678275177242 27.940365876820657 30.316457683733983 20.798498164268125 52-53 20.92886319085181 28.133938046649646 30.56877894488518 20.368419817613358 54-55 20.74047977514924 28.225028183388034 30.45268827954481 20.581803761917918 56-57 21.29396691550437 27.77800629143476 29.789049811564244 21.13897698149663 58-59 21.08139971891512 27.892061890232217 29.71841870156521 21.30811968928745 60-61 21.391011255136263 27.887609466740553 29.916169933756713 20.805209344366467 62-63 20.233134181168626 29.44082219779461 29.426825853457938 20.899217767578822 64-65 20.376621409159103 29.10734708333649 29.573104993473816 20.942926514030592 66-67 20.968004884994464 27.787440852277573 30.18618163752328 21.058372625204687 68-69 21.465360157069902 28.474170384624625 29.195323504157024 20.86514595414845 70-71 20.836304986413438 29.28082754421448 28.95242342415959 20.9304440452125 72-73 20.711730034289204 29.031326542993334 29.481940888660397 20.775002534057066 74-75 20.872904208446773 28.330154094471773 29.672371100323552 21.12457059675791 76-77 21.494651845901735 28.2979337599691 29.197828663710162 21.009585730419 78-79 21.172425043253444 29.175135701691524 28.60968973627796 21.042749518777075 80-81 21.018750644428504 29.203006650110336 29.30215208053708 20.476090624924083 82-83 20.826914116952874 29.033673087181743 29.235595712833735 20.903817083031647 84-85 21.021902103652877 29.303522534749394 28.854263454598154 20.820311906999574 86-87 21.464413110951227 29.426130947538994 28.148221315509197 20.96123462600058 88-89 21.041285989907056 29.543417615563477 28.542326926828203 20.872969467701267 90-91 21.254321284315708 29.477036280948553 28.501312944529317 20.76732949020642 92-93 21.093820950072466 29.649395472916297 28.362689739805624 20.894093837205617 94-95 21.172065245422093 29.84893928357259 28.042989428688614 20.936006042316706 96-97 21.233688032570555 29.249417308185933 28.412179584434877 21.104715074808635 98-99 21.72399641500339 29.609133895700495 27.750936104340706 20.91593358495541 100-101 21.83896631941005 30.29325940389893 27.043329620680705 20.824444656010314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 35219.0 1 23321.0 2 9873.5 3 8713.0 4 10456.0 5 14032.5 6 15420.5 7 15100.0 8 23608.5 9 26665.5 10 68629.0 11 103649.0 12 69866.5 13 59353.5 14 56389.0 15 56556.0 16 97019.5 17 107296.5 18 108986.0 19 112083.5 20 110831.0 21 118378.5 22 117799.0 23 141690.5 24 187411.0 25 229630.5 26 266179.0 27 324695.0 28 412537.0 29 503011.0 30 623178.0 31 774893.5 32 945447.0 33 1173649.5 34 1459400.0 35 1797716.0 36 2128406.0 37 2240208.5 38 2096309.5 39 1871556.5 40 1697973.5 41 1618595.5 42 1582354.5 43 1564764.5 44 1536348.5 45 1511445.0 46 1493121.5 47 1465486.0 48 1446755.0 49 1409757.0 50 1349396.0 51 1291485.0 52 1209099.5 53 1109392.0 54 1008955.5 55 907140.0 56 800521.5 57 678363.5 58 563839.5 59 457699.5 60 353659.0 61 268946.0 62 207391.0 63 153286.5 64 117000.0 65 91821.5 66 64078.0 67 45047.5 68 35715.5 69 29259.0 70 20933.5 71 12593.5 72 8268.0 73 6103.5 74 4747.5 75 3394.0 76 2151.0 77 1575.5 78 1240.0 79 893.0 80 627.5 81 530.5 82 483.5 83 474.5 84 438.5 85 350.5 86 295.5 87 197.5 88 72.0 89 28.0 90 16.0 91 11.0 92 5.0 93 2.0 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06099411200042417 2 0.019029733045468414 3 0.0 4 0.0 5 0.0 6 0.00837406772444427 7 0.14281144053489425 8 0.003157068313226316 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.254205528460993E-5 18-19 0.005487925131714682 20-21 0.0 22-23 8.049404670956459E-4 24-25 8.082990504075886E-4 26-27 0.001246034408730812 28-29 6.112621627736058E-4 30-31 1.5337530457872528E-4 32-33 5.709591630302912E-5 34-35 0.0018886433490825517 36-37 3.9407377526796565E-4 38-39 4.478111082590519E-6 40-41 0.0010266069656838764 42-43 7.59039828499093E-4 44-45 8.049404670956459E-4 46-47 6.101426350029582E-4 48-49 1.5113624903743E-4 50-51 7.735936895175123E-4 52-53 1.880806654688018E-4 54-55 0.025238634061480168 56-57 0.35627963725867234 58-59 0.1165014183969043 60-61 0.3189713743018401 62-63 5.821544407367675E-5 64-65 1.6569011005584922E-4 66-67 0.0018785675991467227 68-69 6.694776068472826E-4 70-71 2.2054697081758307E-4 72-73 2.3510083183600223E-4 74-75 0.0012964131584099552 76-77 5.608834130944625E-4 78-79 4.802774136078332E-4 80-81 5.149827744979097E-5 82-83 0.003385451978438433 84-85 9.40403327344009E-5 86-87 2.2390555412952594E-6 88-89 0.0 90-91 1.1755041591800111E-4 92-93 0.0 94-95 2.3510083183600226E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.4661688E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.12783395582618 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32235133820002 40.26385490822824 2 10.816850650305353 10.844505866469543 3 3.32978205409169 5.007442857497942 4 1.5985220932296877 3.2052180025654993 5 0.924420461374519 2.3169597696575064 6 0.5600496265121876 1.6844444810895243 7 0.4191718266222364 1.4708523006715881 8 0.2871330812304399 1.1514687535315393 9 0.22856236130178484 1.0311602486298679 >10 1.3060435961957997 12.290254380806987 >50 0.12076861294751506 4.186541739708659 >100 0.07679414627613212 7.298759474486678 >500 0.00581578095088034 1.983579473221152 >1k 0.003251532644640287 3.1828438021730308 >5k 2.5036055915821776E-4 0.8868076146298894 >10k+ 2.3247755795574945E-4 3.195306326632039 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116192 0.2601603414541788 No Hit GTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTT 74555 0.1669327858812681 No Hit GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 71218 0.1594610575399658 No Hit TTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTT 65520 0.1467029190656654 No Hit CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 65152 0.14587894662646875 No Hit CGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTT 60248 0.1348986182519568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52018 0.11647119114709681 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC 49353 0.11050410812954495 TruSeq Adapter, Index 8 (100% over 50bp) CGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48849 0.10937562413673213 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.291746071039679E-4 2.2390555412952594E-6 0.0 2.2390555412952594E-6 0.0 2 6.717166623885778E-4 8.956222165181038E-6 0.0 1.1195277706476298E-5 2.2390555412952594E-6 3 7.456054952513215E-4 1.1195277706476298E-5 0.0 1.3434333247771558E-5 2.2390555412952594E-6 4 9.919016047938E-4 1.1195277706476298E-5 0.0 2.9107722036838374E-5 6.717166623885779E-6 5 0.0010904200486107913 1.1195277706476298E-5 0.0 2.9107722036838374E-5 6.717166623885779E-6 6 0.0012381977143362786 1.1195277706476298E-5 0.0 3.806394420201941E-5 6.717166623885779E-6 7 0.0012964131584099552 1.1195277706476298E-5 0.0 4.030299974331467E-5 6.717166623885779E-6 8 0.0014419517685941472 1.1195277706476298E-5 0.0 4.702016636720045E-5 6.717166623885779E-6 9 0.001621076211897768 1.3434333247771558E-5 0.0 4.925922190849571E-5 6.717166623885779E-6 10-11 0.002213306402570364 1.3434333247771558E-5 0.0 5.26178052204386E-5 6.717166623885779E-6 12-13 0.0031290801189601254 1.4553861018419189E-5 0.0 6.157402738561964E-5 6.717166623885779E-6 14-15 0.004131057473689754 1.567338878906682E-5 0.0 7.16497773214483E-5 6.717166623885779E-6 16-17 0.005350223215925023 1.567338878906682E-5 0.0 8.284505502792461E-5 6.717166623885779E-6 18-19 0.006872780984005799 1.567338878906682E-5 0.0 9.180127719310565E-5 6.717166623885779E-6 20-21 0.008531921140105586 1.679291655971445E-5 0.0 1.0411608267022957E-4 7.836694394533408E-6 22-23 0.010213451851618326 1.7912444330362076E-5 0.0 1.1419183260605824E-4 1.0075749935828668E-5 24-25 0.011963273757140572 2.4629610954247856E-5 0.0 1.343433324777156E-4 1.2314805477123928E-5 26-27 0.013652641163047846 2.4629610954247856E-5 0.0 1.466581379548395E-4 1.5673388789066815E-5 28-29 0.015486427651368663 2.4629610954247856E-5 0.0 1.578534156613158E-4 2.4629610954247856E-5 30-31 0.017235130029120263 3.358583311942889E-5 0.0 1.6009247120261105E-4 2.6868666495543117E-5 32-33 0.019076753211835613 3.358583311942889E-5 0.0 1.6233152674390632E-4 2.6868666495543117E-5 34-35 0.02093293025556938 3.358583311942889E-5 0.0 1.768853877623255E-4 2.6868666495543117E-5 36-37 0.02301189332566203 3.358583311942889E-5 0.0 1.8920019323944944E-4 2.6868666495543117E-5 38-39 0.025332674394214567 3.358583311942889E-5 0.0 1.9815641540463046E-4 2.6868666495543117E-5 40-41 0.02790087110008023 3.582488866072415E-5 0.0 2.0935169311110677E-4 2.6868666495543117E-5 42-43 0.030826197164782486 3.6944416431371785E-5 0.0 2.183079152762878E-4 2.6868666495543117E-5 44-45 0.03424859355965229 3.806394420201941E-5 0.0 2.2390555412952595E-4 2.6868666495543117E-5 46-47 0.03831359889487383 3.918347197266704E-5 0.0 2.3733988737729752E-4 2.6868666495543117E-5 48-49 0.0431477198085303 4.030299974331467E-5 0.0 2.4069847068924042E-4 2.6868666495543117E-5 50-51 0.049259221908495715 4.1422527513962305E-5 0.0 2.4517658177183096E-4 2.6868666495543117E-5 52-53 0.05707240621984552 4.254205528460993E-5 0.0 2.518937483957167E-4 2.6868666495543117E-5 54-55 0.06611931013444902 4.366158305525756E-5 0.0 2.619694983315454E-4 2.6868666495543117E-5 56-57 0.0778900251150382 4.478111082590519E-5 0.0 2.709257204967264E-4 2.6868666495543117E-5 58-59 0.09320068690641518 4.478111082590519E-5 0.0 2.8547958151514555E-4 2.6868666495543117E-5 60-61 0.11341376080545815 4.478111082590519E-5 0.0 2.921967481390314E-4 2.7988194266190743E-5 62-63 0.13933306775149207 4.478111082590519E-5 0.0 3.056310813868029E-4 3.2466305348781264E-5 64-65 0.1730588418422519 4.478111082590519E-5 0.0 3.1010919246939343E-4 3.582488866072415E-5 66-67 0.2163095582056818 4.5900638596552826E-5 0.0 3.190654146345745E-4 3.582488866072415E-5 68-69 0.27149556013198606 4.702016636720045E-5 0.0 3.35858331194289E-4 3.582488866072415E-5 70-71 0.3437554800884373 4.925922190849571E-5 0.0 3.4929266444206047E-4 3.6944416431371785E-5 72-73 0.4367680863293837 5.149827744979097E-5 0.0 3.593684143778892E-4 4.030299974331467E-5 74-75 0.5544461284132387 5.149827744979097E-5 0.0 3.683246365430702E-4 4.030299974331467E-5 76-77 0.6997877017097965 5.485686076173386E-5 0.0 3.772808587082512E-4 4.030299974331467E-5 78-79 0.879532363398356 5.597638853238149E-5 0.0 3.8287849756148937E-4 4.030299974331467E-5 80-81 1.1018224389548377 6.045449961497201E-5 0.0 3.9295424749731804E-4 4.030299974331467E-5 82-83 1.3778039468638086 6.045449961497201E-5 0.0 3.974323585799086E-4 4.030299974331467E-5 84-85 1.7254150805943564 6.157402738561964E-5 0.0 3.996714141212038E-4 4.030299974331467E-5 86-87 2.1452603851426306 6.269355515626727E-5 0.0 4.030299974331467E-4 4.030299974331467E-5 88-89 2.623545039318711 6.269355515626727E-5 0.0 4.0750810851573725E-4 4.030299974331467E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGC 9085 0.0 44.510563 1 GACGGCC 11965 0.0 35.843678 2 ACGGCCA 13665 0.0 31.308937 3 CGGCCAG 23500 0.0 29.637552 1 TATGCCG 11620 0.0 24.897871 46-47 TGCCGTC 12750 0.0 22.678295 48-49 CGTATGC 13060 0.0 22.425228 44-45 ATGCCGT 12675 0.0 22.22631 46-47 CTCGTAT 12270 0.0 21.954151 42-43 TCTCGTA 11715 0.0 21.758505 40-41 TCGTATG 13605 0.0 21.143135 42-43 GCCGTCT 14115 0.0 20.064709 48-49 CGGCGCT 4795 0.0 19.545498 1 CCGTCTT 15100 0.0 19.195227 50-51 CGGCGAG 3800 0.0 18.90438 1 AATCTCG 13755 0.0 18.773071 38-39 CTTTTAT 75415 0.0 18.369728 1 CGTCTTC 15875 0.0 18.003094 50-51 GCGAACT 2640 0.0 17.479818 1 CCGACGG 2255 0.0 17.299614 1 >>END_MODULE