FastQCFastQC Report
Fri 27 May 2016
SRR1233105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25975846
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC310560.1195572225058618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCC75800.028.7802092
CGACGGC78000.027.2955991
CTGGAAT473700.024.3262441
ACGGCCA120850.018.2904763
TATGGCG544650.018.2395827
AACGACG28500.018.225062
CGGCCAG125150.017.9173361
TGGCGAG560150.017.4901589
ATGGCGA575200.017.3895938
GAATATG672850.017.3694444
GGAATAT731750.016.7817173
TGGAATA747900.016.4722022
CGCCGTA81050.014.68189846-47
GTTTTTT826450.014.4858291
GGCGAGA331750.014.32071410-11
CTCCGTT51850.014.203211
GCGAGAA326950.013.88722710-11
CTTTTAT406050.013.7768361
ATATGGC909300.013.6466236
CGAGAAA360400.013.2578712-13
Produced by FastQC (version 0.11.5)