FastQCFastQC Report
Fri 27 May 2016
SRR1233105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25975846
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC345370.13295813349062818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCC78650.029.0487382
CTGGAAT494100.025.6456641
CGACGGC86200.024.9052011
CGGCCAG139950.020.9181351
AACGACG30900.018.9072172
ACGGCCA122600.018.8289783
TATGGCG586350.018.0889247
GAATATG725600.017.9952724
GGAATAT786300.017.5061663
TGGCGAG602150.017.409199
ATGGCGA619000.017.2729238
TGGAATA795450.017.2092532
GGCGAGA348350.014.63750910-11
GCGAGAA348700.014.30270910-11
CGGGGTT49200.014.1256521
CCCCGAT31050.014.1041511
ATATGGC982450.013.8611156
CTTTTAT393200.013.8252611
GTTTTTT771800.013.74140551
CGGGGAT32000.013.6854341
Produced by FastQC (version 0.11.5)