FastQCFastQC Report
Fri 27 May 2016
SRR1233104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76007435
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2833670.37281484370575063No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1024000.13472366223120147No Hit
GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA887800.11680436262584049No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC883780.11627546699872189No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC816260.10739212552035204No Hit
ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT809090.10644879675258084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAC4277500.025.6069241
GCGTCGG79950.020.02790894-95
GAAAAAG5051450.019.5401959
CGGCGAG60350.017.926451
ACGTGGA2265300.017.77357544-45
CTGGAAT2030300.017.1870421
TATGGCA3290750.016.51749252-53
GACGTGG2136450.016.34687444-45
TGGCAAG3332350.016.07554-55
AAACTGA9447550.015.90704254
GAAAACT9249450.015.848322
AACTGAA9509800.015.8145975
AAAACTG9453900.015.7301933
GCAAGAA3450150.015.69093656-57
ACTGAAA9979450.015.2075166
CGTGGAA2333400.015.18723446-47
TGTAGGA3522550.015.08479938-39
ACTTGAC2859750.014.85693394-95
GTGGAAT2908800.014.36106546-47
CCACTTG3049600.014.2543292-93
Produced by FastQC (version 0.11.5)