FastQCFastQC Report
Fri 27 May 2016
SRR1233104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76007435
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG2692100.35418903427013426No Hit
AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA1020680.13428686285756125No Hit
ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT978640.1287558250058037No Hit
CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC946160.12448255884440779No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC907440.11938832036629049No Hit
GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA871640.11467825483125434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAC4245950.023.9285851
GAAAAAG4965000.018.8358739
CGGCGAG62650.018.78741
CTGGAAT2071350.017.1526971
ACGTGGA2255700.016.7450844-45
CGGCGCT60950.016.196671
TATGGCA3270300.015.64957752-53
GACGTGG2125750.015.60317444-45
TGGCAAG3333250.015.19283854-55
AACTGAA9479800.015.1238475
AAACTGA9389400.015.0914694
GAAAACT9220050.014.9472522
GCAAGAA3440350.014.89057556-57
AAAACTG9400550.014.8330613
CTTTTAT1114200.014.7340971
ATACGAC1410050.014.671971
ACTGAAA9928000.014.5615396
TACGACT1431650.014.4392312
CGTGGAA2318050.014.43679646-47
ACGACTC1451650.014.3283013
Produced by FastQC (version 0.11.5)