##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233104_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76007435 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27199735131175 31.0 31.0 34.0 28.0 34.0 2 31.518154993652924 31.0 31.0 34.0 28.0 34.0 3 31.18753998210833 31.0 31.0 34.0 27.0 34.0 4 34.34874480371558 37.0 35.0 37.0 30.0 37.0 5 34.695647392916236 37.0 35.0 37.0 30.0 37.0 6 35.035578427294645 37.0 35.0 37.0 32.0 37.0 7 35.32976708133882 37.0 35.0 37.0 33.0 37.0 8 35.3384257079587 37.0 35.0 37.0 33.0 37.0 9 36.91603613514915 39.0 37.0 39.0 33.0 39.0 10-11 36.83324731981812 39.0 37.0 39.0 32.5 39.0 12-13 36.66343014995836 39.0 37.0 39.0 32.0 39.0 14-15 38.06382583493312 40.0 37.0 41.0 33.0 41.0 16-17 38.0308221952234 40.0 37.0 41.0 33.0 41.0 18-19 38.056913958483136 40.0 37.0 41.0 33.0 41.0 20-21 38.050885903464575 40.0 37.0 41.0 33.0 41.0 22-23 38.06492918094131 40.0 37.0 41.0 33.0 41.0 24-25 38.02528286870883 40.0 37.0 41.0 33.0 41.0 26-27 37.77141042846664 40.0 37.0 41.0 32.0 41.0 28-29 37.64019523879473 40.0 37.0 41.0 32.0 41.0 30-31 37.52433809271422 40.0 37.0 41.0 32.0 41.0 32-33 37.47211483323967 40.0 37.0 41.0 32.0 41.0 34-35 37.37500626090066 40.0 36.0 41.0 31.0 41.0 36-37 37.23226900105233 39.0 36.0 41.0 31.0 41.0 38-39 37.143404188024505 39.0 36.0 41.0 31.0 41.0 40-41 37.013286739645935 39.0 36.0 41.0 30.5 41.0 42-43 36.90957033348119 39.0 36.0 41.0 30.0 41.0 44-45 36.73552346556623 39.0 35.5 40.0 30.0 41.0 46-47 36.59162401678204 39.0 35.0 40.0 30.0 41.0 48-49 36.44665994188595 39.0 35.0 40.0 30.0 41.0 50-51 36.26386091044909 39.0 35.0 40.0 29.5 41.0 52-53 35.964910570919805 38.0 35.0 40.0 28.5 41.0 54-55 35.851931288037804 38.5 34.5 40.0 28.0 41.0 56-57 35.85096255386068 38.5 35.0 40.0 28.0 41.0 58-59 35.67326619428744 38.0 34.0 40.0 28.0 41.0 60-61 35.402539804165215 38.0 34.0 40.0 27.5 41.0 62-63 35.10372116885671 38.0 34.0 40.0 26.5 41.0 64-65 34.82674079187122 37.5 34.0 40.0 26.0 41.0 66-67 34.62352834298382 37.0 33.5 40.0 26.0 41.0 68-69 34.64946775272709 37.0 34.0 40.0 27.0 41.0 70-71 34.35769461237575 36.5 33.5 39.0 26.0 41.0 72-73 34.08758714196841 36.0 33.0 39.0 26.0 41.0 74-75 33.756822447698696 36.0 33.0 39.0 26.0 40.0 76-77 32.66254157899158 35.0 31.5 37.0 25.5 39.0 78-79 32.990197860248806 35.0 32.0 37.0 26.0 39.0 80-81 32.83508481111091 35.0 32.0 37.0 26.0 39.0 82-83 32.57089718262431 35.0 32.0 36.0 26.0 39.0 84-85 32.285939046620896 35.0 32.0 36.0 26.0 37.0 86-87 31.925270646220334 35.0 32.0 35.5 25.5 37.0 88-89 31.63154274973231 35.0 32.0 35.0 25.0 36.5 90-91 31.422754537105483 35.0 32.0 35.0 25.0 36.0 92-93 31.19475791414353 34.0 32.0 35.0 24.0 36.0 94-95 30.99002026578058 34.0 32.0 35.0 23.5 36.0 96-97 30.721585171240157 34.0 32.0 35.0 21.0 35.5 98-99 30.39942539831794 34.0 31.0 35.0 18.5 35.0 100-101 29.070752381000624 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2599947050775917 1101 2 1.2522560877229658 1101 3 1.6668714430857996 1101 4 2.0094331575502054 1101 5 1.596751711563364 1101 6 1.013876087131031 1101 7 0.7426157992236639 1101 8 0.6612507985625626 1101 9 0.7483491185275071 1101 10-11 0.8429388789583143 1101 12-13 1.2010494299178518 1101 14-15 1.0702858510990794 1101 16-17 0.8801685409994704 1101 18-19 0.8634822511187394 1101 20-21 0.9058084272519551 1101 22-23 0.82548952254988 1101 24-25 0.8476707141845736 1101 26-27 0.9245968188105707 1101 28-29 0.9220563758242477 1101 30-31 0.9514425520768199 1101 32-33 0.9859641208919925 1101 34-35 0.887838283517361 1101 36-37 0.8452134855182294 1101 38-39 0.8388197386616909 1101 40-41 0.8768550483572426 1101 42-43 0.9002462132856692 1101 44-45 0.905219107994661 1101 46-47 0.8765076419856328 1101 48-49 0.8654668590859913 1101 50-51 0.9268662350753303 1101 52-53 1.003145914519223 1101 54-55 1.0650733266580943 1101 56-57 1.038832993856552 1101 58-59 0.8901996578900935 1101 60-61 0.9963528207263224 1101 62-63 1.0939698788849412 1101 64-65 1.172546819800921 1101 66-67 0.9661979163388992 1101 68-69 0.9966153449354564 1101 70-71 1.1700348384491193 1101 72-73 0.9675710546046474 1101 74-75 0.8763193466514636 1101 76-77 0.8422165676875508 1101 78-79 0.8781287643961733 1101 80-81 0.8783851155999116 1101 82-83 0.8305854782255082 1101 84-85 0.8104373879135593 1101 86-87 0.7951871760408658 1101 88-89 0.792757600396488 1101 90-91 0.7709777104014002 1101 92-93 0.8044535917072508 1101 94-95 0.757919254644829 1101 96-97 0.7269540453645114 1101 98-99 0.8887704487570218 1101 100-101 1.0003584086413326 1102 1 1.1820336820002488 1102 2 1.155071847871472 1102 3 1.5798823285022756 1102 4 1.9452466495709757 1102 5 1.5259134227767959 1102 6 0.9760471741561005 1102 7 0.6832761826930422 1102 8 0.5677279236688264 1102 9 0.7039289004553453 1102 10-11 0.7585835561690928 1102 12-13 1.1185651167167023 1102 14-15 0.9533460241943814 1102 16-17 0.7501769950377835 1102 18-19 0.6777199281303439 1102 20-21 0.810197251708388 1102 22-23 0.7260791020551736 1102 24-25 0.7514487871043158 1102 26-27 0.7937178431689063 1102 28-29 0.8296785966332436 1102 30-31 0.9096759454187051 1102 32-33 0.9154448441430034 1102 34-35 0.8692593030793532 1102 36-37 0.798046277220962 1102 38-39 0.8019018831926843 1102 40-41 0.7408945733346002 1102 42-43 0.7634579528680376 1102 44-45 0.8358319381739179 1102 46-47 0.8152925955563788 1102 48-49 0.7519411259241551 1102 50-51 0.8346696163405412 1102 52-53 0.9259250198057885 1102 54-55 1.0352896934024045 1102 56-57 0.9366619987069598 1102 58-59 0.857068446895191 1102 60-61 0.8590882977671086 1102 62-63 0.9589497995096465 1102 64-65 1.088286001337103 1102 66-67 0.8542462738065311 1102 68-69 0.9084360552575461 1102 70-71 1.0269895661469093 1102 72-73 0.8903813707329746 1102 74-75 0.845142646147039 1102 76-77 0.7727924792018399 1102 78-79 0.8133763156039393 1102 80-81 0.7551616153795777 1102 82-83 0.7778168078426404 1102 84-85 0.6899776554592236 1102 86-87 0.6121122518289575 1102 88-89 0.5793602335912276 1102 90-91 0.6804090365125894 1102 92-93 0.756746145995006 1102 94-95 0.6799006454085905 1102 96-97 0.6800110895698062 1102 98-99 0.7814422511745569 1102 100-101 0.8796493473444613 1103 1 1.1015142476703979 1103 2 1.1205435426742234 1103 3 1.54253020143641 1103 4 1.9191539937113262 1103 5 1.5083688668003035 1103 6 0.9425699500638061 1103 7 0.6317426894297213 1103 8 0.518963428942584 1103 9 0.6161976115773342 1103 10-11 0.6627624557697445 1103 12-13 1.0413619891919055 1103 14-15 0.8341621049828305 1103 16-17 0.7306034476645635 1103 18-19 0.7110624604301492 1103 20-21 0.7788246048763199 1103 22-23 0.6875891841551223 1103 24-25 0.5935071058051875 1103 26-27 0.6796681570720224 1103 28-29 0.7235049612438047 1103 30-31 0.8056673286331488 1103 32-33 0.7976344948704295 1103 34-35 0.7291237353041566 1103 36-37 0.7133307936231859 1103 38-39 0.7234773175679194 1103 40-41 0.7341759298310819 1103 42-43 0.7641178573835532 1103 44-45 0.7890179407053566 1103 46-47 0.7686421442358338 1103 48-49 0.7497053750938889 1103 50-51 0.7021763700027108 1103 52-53 0.8088835904699891 1103 54-55 0.9475845087951029 1103 56-57 0.8576173153739646 1103 58-59 0.70671559055058 1103 60-61 0.7875069745341889 1103 62-63 0.8776354579341614 1103 64-65 0.8773144456786142 1103 66-67 0.7111824032752168 1103 68-69 0.7730347573776939 1103 70-71 0.9888678295415971 1103 72-73 0.8801373390457101 1103 74-75 0.743005116158777 1103 76-77 0.6562505031196864 1103 78-79 0.7669718791923472 1103 80-81 0.7336840047684348 1103 82-83 0.8029299455651611 1103 84-85 0.7118526393169233 1103 86-87 0.6938113198793552 1103 88-89 0.5249376430121835 1103 90-91 0.6652795299797312 1103 92-93 0.731450848296415 1103 94-95 0.7639809425113064 1103 96-97 0.7579518043812712 1103 98-99 0.841413009706482 1103 100-101 0.8655328103617919 1104 1 1.0037730788555272 1104 2 1.0401108483589532 1104 3 1.470768944718035 1104 4 1.8095684006700878 1104 5 1.438457673040105 1104 6 0.8952651058987371 1104 7 0.5862935073777393 1104 8 0.4996854918635023 1104 9 0.5415765930123158 1104 10-11 0.6118417453547806 1104 12-13 0.9568714585391618 1104 14-15 0.7556549098437344 1104 16-17 0.6194245147767106 1104 18-19 0.5913201939695298 1104 20-21 0.6538818472162404 1104 22-23 0.48282578136153376 1104 24-25 0.5369250494731403 1104 26-27 0.700362942423034 1104 28-29 0.7109122809514687 1104 30-31 0.7557258613732856 1104 32-33 0.7310615234499664 1104 34-35 0.6219090567034726 1104 36-37 0.585623642443295 1104 38-39 0.5517201873311919 1104 40-41 0.5448206807361089 1104 42-43 0.5950894541563798 1104 44-45 0.6221497718921967 1104 46-47 0.5383785264146965 1104 48-49 0.45978510587618615 1104 50-51 0.6492819155148624 1104 52-53 0.7555965709876489 1104 54-55 0.8728175944177821 1104 56-57 0.7150982263682906 1104 58-59 0.5971050016733628 1104 60-61 0.6671610830068246 1104 62-63 0.6759417188690335 1104 64-65 0.8156208234416695 1104 66-67 0.6186367596829712 1104 68-69 0.6644790051844112 1104 70-71 0.7970294936566873 1104 72-73 0.618883639818641 1104 74-75 0.46958701759789534 1104 76-77 0.377085267374099 1104 78-79 0.561268397470549 1104 80-81 0.5669451871266133 1104 82-83 0.6166874580451633 1104 84-85 0.569624953172088 1104 86-87 0.5623026354636558 1104 88-89 0.5608293890584015 1104 90-91 0.5613859376384518 1104 92-93 0.5576833560507239 1104 94-95 0.5444524811392277 1104 96-97 0.5862977682157684 1104 98-99 0.6377765477413426 1104 100-101 0.6109265911005863 1105 1 0.9843321038495922 1105 2 0.9957115078882843 1105 3 1.4088630032205707 1105 4 1.8041384323605456 1105 5 1.4069023467854578 1105 6 0.8470322768671963 1105 7 0.5411036157569313 1105 8 0.4397651457501226 1105 9 0.5053709347275444 1105 10-11 0.584127857665429 1105 12-13 0.9576927761001386 1105 14-15 0.7254254820527777 1105 16-17 0.5553193391663669 1105 18-19 0.5146596448368754 1105 20-21 0.6175684734275677 1105 22-23 0.5202219049074088 1105 24-25 0.48437078810709266 1105 26-27 0.5909724243589167 1105 28-29 0.6208758439877045 1105 30-31 0.6756949966118455 1105 32-33 0.6566432099149537 1105 34-35 0.5849438436650445 1105 36-37 0.5706287622507205 1105 38-39 0.5415063880006841 1105 40-41 0.5496737441527131 1105 42-43 0.5943109500912698 1105 44-45 0.6314599826447491 1105 46-47 0.5363323981593524 1105 48-49 0.5161146057386716 1105 50-51 0.6234093095054689 1105 52-53 0.6805123439818743 1105 54-55 0.7429782023967135 1105 56-57 0.682021597107159 1105 58-59 0.6152210806017067 1105 60-61 0.5907067187357242 1105 62-63 0.7719762098681642 1105 64-65 0.8391512313604679 1105 66-67 0.6275910486813743 1105 68-69 0.6246649200323517 1105 70-71 0.7884796443789455 1105 72-73 0.675957136525895 1105 74-75 0.6048235015691361 1105 76-77 0.49811312977877975 1105 78-79 0.5236676775583291 1105 80-81 0.5562665208972248 1105 82-83 0.5854103200942617 1105 84-85 0.47853268444043096 1105 86-87 0.4699753851512831 1105 88-89 0.49214326526754704 1105 90-91 0.4971104668840489 1105 92-93 0.5167002798459848 1105 94-95 0.5004532054215893 1105 96-97 0.5311291354361458 1105 98-99 0.6244155020293967 1105 100-101 0.6397762693924349 1106 1 0.9097311018916763 1106 2 0.9444732312131343 1106 3 1.3619754576561895 1106 4 1.7660438379955536 1106 5 1.349297889569229 1106 6 0.792374924966758 1106 7 0.5054687419054815 1106 8 0.3564724820994414 1106 9 0.4420889439448601 1106 10-11 0.5241803486122834 1106 12-13 0.8341429627160082 1106 14-15 0.6320401739706014 1106 16-17 0.4744549995803169 1106 18-19 0.44015453676120586 1106 20-21 0.5090831321469977 1106 22-23 0.431675106185466 1106 24-25 0.42339062185838827 1106 26-27 0.49321312526237193 1106 28-29 0.5046204619361987 1106 30-31 0.5845350159162521 1106 32-33 0.5509024678028069 1106 34-35 0.44672500167947504 1106 36-37 0.4735358090574806 1106 38-39 0.4476824771739629 1106 40-41 0.42932092485307294 1106 42-43 0.47525740934172234 1106 44-45 0.5059620379393053 1106 46-47 0.48102284805002427 1106 48-49 0.4559970659130883 1106 50-51 0.5492749988725691 1106 52-53 0.6080398439674468 1106 54-55 0.7116746738581696 1106 56-57 0.6498292651929631 1106 58-59 0.5785634907017752 1106 60-61 0.601963435071859 1106 62-63 0.776708740455895 1106 64-65 0.8057408565968771 1106 66-67 0.6275515794625335 1106 68-69 0.5795396685943999 1106 70-71 0.7356556732849171 1106 72-73 0.631413550212713 1106 74-75 0.465714156927838 1106 76-77 0.35867759054752213 1106 78-79 0.462762158757954 1106 80-81 0.5485041791923706 1106 82-83 0.5562839698090798 1106 84-85 0.466166257750821 1106 86-87 0.5235396480677714 1106 88-89 0.5180264628654534 1106 90-91 0.5155497592838998 1106 92-93 0.5215168674492681 1106 94-95 0.4719935001022115 1106 96-97 0.499520261388259 1106 98-99 0.5322136526440104 1106 100-101 0.520605656931437 1107 1 0.8125390676518514 1107 2 0.8718485885887084 1107 3 1.301191542296511 1107 4 1.7384895702794907 1107 5 1.3213171575371163 1107 6 0.7241527064388507 1107 7 0.4095384589818849 1107 8 0.32485167956009775 1107 9 0.4171535281934666 1107 10-11 0.40021405979604197 1107 12-13 0.7877653686833028 1107 14-15 0.48774518726925464 1107 16-17 0.398309030184798 1107 18-19 0.29181079635638696 1107 20-21 0.3596537795614623 1107 22-23 0.3250344906759679 1107 24-25 0.3277395535419316 1107 26-27 0.44921506969902225 1107 28-29 0.4457166335637339 1107 30-31 0.4560819705942194 1107 32-33 0.451948190394603 1107 34-35 0.3635071400441774 1107 36-37 0.33304496781424575 1107 38-39 0.3468957711802503 1107 40-41 0.2919166065647545 1107 42-43 0.3048728991343097 1107 44-45 0.3914644246404535 1107 46-47 0.35333089100240755 1107 48-49 0.35327025868937056 1107 50-51 0.3999469230267394 1107 52-53 0.4803896259084013 1107 54-55 0.5529734807832938 1107 56-57 0.33697306951123096 1107 58-59 0.342371249452583 1107 60-61 0.34143583132068756 1107 62-63 0.5566358124922104 1107 64-65 0.5422653780774098 1107 66-67 0.34978739973057316 1107 68-69 0.40529060117322757 1107 70-71 0.5978683530059143 1107 72-73 0.4628600589638765 1107 74-75 0.33142342982217343 1107 76-77 0.21024356262132216 1107 78-79 0.38992996893322385 1107 80-81 0.3582924186920238 1107 82-83 0.3856416328130692 1107 84-85 0.4324799015954994 1107 86-87 0.38378151496849355 1107 88-89 0.3759128164285066 1107 90-91 0.3662732028419775 1107 92-93 0.4038707127104857 1107 94-95 0.36796223622046753 1107 96-97 0.3534166435315562 1107 98-99 0.3925478792604231 1107 100-101 0.41886437465498005 1108 1 0.7663483224650953 1108 2 0.8097478062238999 1108 3 1.2187948942696103 1108 4 1.6337636067641839 1108 5 1.267599595234799 1108 6 0.7081725855621457 1108 7 0.3633848379192486 1108 8 0.23221822764414668 1108 9 0.22615242156315674 1108 10-11 0.26318970783606943 1108 12-13 0.6670937125606571 1108 14-15 0.32115307087099154 1108 16-17 0.25183036860418895 1108 18-19 0.1613110739579824 1108 20-21 0.2131446703697577 1108 22-23 0.07946374064817263 1108 24-25 0.2346999839564603 1108 26-27 0.3131153784616316 1108 28-29 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94-95 0.2486914878569415 1108 96-97 0.1867813069919002 1108 98-99 0.1845386008810017 1108 100-101 0.20153865439596785 1109 1 0.7114156548363653 1109 2 0.7500519463530644 1109 3 1.1471700116810943 1109 4 1.5646440014228133 1109 5 1.195939634497492 1109 6 0.6460718423289435 1109 7 0.3680297230140255 1109 8 0.24339567355684721 1109 9 0.22733176707782832 1109 10-11 0.22844308177916872 1109 12-13 0.6536112596761186 1109 14-15 0.3662182058820562 1109 16-17 0.1909309524491647 1109 18-19 0.13358143906848596 1109 20-21 0.19027933017620313 1109 22-23 0.07996598076136507 1109 24-25 0.19482134352372782 1109 26-27 0.2937468265395182 1109 28-29 0.22764203673530403 1109 30-31 0.25791745744430017 1109 32-33 0.2436243751332583 1109 34-35 0.12418248480231853 1109 36-37 0.08874390308622537 1109 38-39 0.10072724255463328 1109 40-41 0.05127272905696145 1109 42-43 0.10254301486438777 1109 44-45 0.1490300685110313 1109 46-47 0.11940102223857707 1109 48-49 0.022339029836480506 1109 50-51 0.18156508583157205 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2316 94-95 -7.065617739404207 2316 96-97 -7.960510899509135 2316 98-99 -7.41686621286021 2316 100-101 -6.7876663881549675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18948.0 3 2383.0 4 3361.0 5 5288.0 6 9143.0 7 17495.0 8 32901.0 9 49684.0 10 66572.0 11 84493.0 12 103995.0 13 128176.0 14 157468.0 15 190594.0 16 220393.0 17 244310.0 18 260775.0 19 282585.0 20 312221.0 21 284611.0 22 311557.0 23 366662.0 24 457968.0 25 592108.0 26 781225.0 27 1019000.0 28 1304586.0 29 1643610.0 30 2048557.0 31 2525163.0 32 3106531.0 33 3841467.0 34 4869243.0 35 6415167.0 36 9137537.0 37 1.4389015E7 38 1.638281E7 39 4330022.0 40 9811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.83100882253298 22.015200962027123 19.150553904700335 26.003236310739563 2 20.92941068801727 24.248334983719282 42.36482965448833 12.457424673775115 3 20.979160623910083 29.542851973406968 34.83857758428443 14.639409818398516 4 19.560431347939613 36.841858320468184 28.225428039852822 15.372282291739376 5 20.416783360936268 38.926980912828085 26.543358490177294 14.112877236058349 6 16.553474012114737 38.940057640413734 26.142379360650704 18.36408898682083 7 15.24207862033497 15.995587800061928 48.79413573158995 19.96819784801316 8 18.146119521070535 22.98633416586154 35.021387052464014 23.846159260603912 9 21.014671788358598 22.352293561807475 32.88713663340961 23.745898016424313 10-11 21.585512654123903 35.905951174381826 24.090792565227336 18.41774360626694 12-13 18.650551315913972 28.77492135338261 33.86873156802771 18.7057957626757 14-15 19.319444209635545 28.138018339916353 34.069269670789446 18.473267779658663 16-17 20.847457883560995 27.90126255411724 31.370022551083327 19.88125701123844 18-19 22.660052927716873 28.838951084193276 28.49234157158441 20.00865441650544 20-21 20.607151787190293 30.52036488272496 29.38179205757963 19.490691272505117 22-23 19.887026183688477 30.618632900847135 31.62005993229478 17.87428098316961 24-25 20.227495069634863 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29.653094904985767 20.061866708887802 50-51 20.517323981498116 29.78694290085386 30.120054824968484 19.57567829267954 52-53 19.67360556859969 30.10101474335879 31.158208757604704 19.067170930436813 54-55 19.885888187061937 29.841163531463717 30.829054988259035 19.44389329321531 56-57 21.541476403872384 28.360979477278157 29.564011277478798 20.533532841370654 58-59 20.656986741475908 29.513614498751632 28.94162296365101 20.887775796121446 60-61 21.41241435948738 29.517891551037028 29.36654665501405 19.70314743446154 62-63 18.666704040759143 32.23404780396968 29.351096480380924 19.748151674890256 64-65 19.20054223664082 30.7551380088877 29.639638984023993 20.404680770447484 66-67 20.363297319665794 28.27001909093304 30.473247963461414 20.89343562593975 68-69 21.49099012227766 30.287818426957365 28.181730349842898 20.039461100922075 70-71 19.959993133552505 31.802922269649187 28.51460019597518 19.722484400823127 72-73 19.542055198602554 30.748227294869938 30.1337206519521 19.575996854575408 74-75 20.217698607856686 29.288890388178178 29.96284760261026 20.53056340135488 76-77 21.44274931897139 29.481878017918568 28.61735006445427 20.45802259865577 78-79 20.87579136046449 31.170960357081245 27.77418233963031 20.179065942823954 80-81 20.06488493026579 31.25779812116596 29.544479691344485 19.132837257223763 82-83 19.793670177926465 30.593461799463423 29.63551222160461 19.9773558010055 84-85 20.588323654126853 30.701388281119197 28.70701073563728 20.003277329116674 86-87 21.39577120543762 31.017061114223292 27.43787829091426 20.149289389424833 88-89 20.40130787210751 31.208999840976936 28.451359235353475 19.938333051562086 90-91 20.464500425361315 30.764294611653092 28.72182267520867 20.049382287776922 92-93 19.9155285466909 30.477456636566945 28.721570600039552 20.8854442167026 94-95 19.899535788006457 30.58240998419372 28.13391332467615 21.384140903123676 96-97 20.437994132420766 29.481699975623876 28.971974605103274 21.10833128685209 98-99 21.500464899143864 30.012327458590004 28.082325683289593 20.404881958976542 100-101 21.07532451180034 31.184330961734215 27.233162988767347 20.507181537698102 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8271.0 1 6152.0 2 4069.0 3 4771.0 4 6302.0 5 8641.0 6 10528.5 7 11398.0 8 14683.5 9 17314.5 10 35851.5 11 52396.0 12 45747.5 13 47641.0 14 53005.0 15 85758.0 16 154400.0 17 179921.5 18 190892.0 19 194123.5 20 191508.0 21 217136.5 22 242874.5 23 300525.0 24 394558.0 25 490791.0 26 583049.5 27 703452.0 28 864146.5 29 1029381.0 30 1240731.0 31 1527856.5 32 1930541.5 33 2513138.5 34 3267769.5 35 4143119.0 36 4894099.5 37 4974886.0 38 4349834.0 39 3581227.5 40 3025305.5 41 2710333.5 42 2537441.5 43 2444038.0 44 2339102.0 45 2250162.5 46 2195786.5 47 2151582.0 48 2113674.0 49 2021563.0 50 1882377.0 51 1750061.5 52 1607826.5 53 1448798.5 54 1284579.5 55 1120295.0 56 964773.5 57 808601.0 58 664644.0 59 530303.5 60 406840.5 61 311370.5 62 235778.0 63 172400.0 64 126552.0 65 91829.5 66 64936.5 67 48154.0 68 35730.0 69 25851.5 70 17853.0 71 11981.0 72 9335.0 73 7566.5 74 5268.5 75 3543.0 76 2673.5 77 2091.0 78 1749.0 79 1418.0 80 1192.5 81 964.0 82 671.5 83 524.5 84 432.5 85 313.5 86 251.5 87 205.0 88 145.0 89 91.5 90 45.5 91 34.5 92 20.0 93 7.5 94 5.0 95 2.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10231499063216645 2 0.001277506601821256 3 6.012569691372956E-4 4 0.005644184677459514 5 5.499461993422091E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.14976692740650965 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01218367650480509 26-27 0.06374968448810303 28-29 0.0332460896753061 30-31 0.023179969170121318 32-33 0.013189499158865182 34-35 0.0046179692815577844 36-37 0.008058422179356532 38-39 0.008989252169869962 40-41 0.0080827619034901 42-43 0.018163354677078632 44-45 0.02156104860004814 46-47 0.022186645293319002 48-49 0.018285711128128452 50-51 0.08267678023867008 52-53 0.236022962753578 54-55 0.24184673512532032 56-57 0.4279462923594251 58-59 0.43601326633374743 60-61 0.40629380533628057 62-63 0.4004483245619327 64-65 0.3853451968218635 66-67 0.34236190183236676 68-69 0.2719622100127442 70-71 0.28378210631630973 72-73 0.2759190597604037 74-75 0.27439157761342686 76-77 0.273094993930528 78-79 0.24579108609572206 80-81 0.2187286020111059 82-83 0.1939126086809797 84-85 0.1290156680066891 86-87 0.04885443114874223 88-89 0.011874496225270594 90-91 0.026669759346569188 92-93 0.022249139179607892 94-95 0.037022693898300874 96-97 0.04947476519895718 98-99 0.048934686455344795 100-101 0.02487125108221321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.6007435E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.16365590509393 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33238655007163 34.15280909917078 2 11.321619604466356 10.000082250000357 3 4.034311721609747 5.345098640611799 4 1.9999843557626993 3.533064836138993 5 1.1998571776909073 2.649503976539919 6 0.8008344217981084 2.122066550474791 7 0.5487906981387995 1.6965622489562755 8 0.3907146074061037 1.3804308382861623 9 0.3230403255273405 1.2839977602053096 >10 1.8022109383154195 14.692157227053102 >50 0.14660525014934916 4.472794013732322 >100 0.08786165313323031 7.369764377793035 >500 0.006867544639044875 2.060295398741703 >1k 0.004154034716635289 3.6126293288883864 >5k 4.0455225782308046E-4 1.274778208797513 >10k+ 3.5656431655543087E-4 4.353965244609332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG 269210 0.35418903427013426 No Hit AGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAA 102068 0.13428686285756125 No Hit ATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97864 0.1287558250058037 No Hit CTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACAC 94616 0.12448255884440779 No Hit CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 90744 0.11938832036629049 No Hit GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA 87164 0.11467825483125434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.183605590689911E-5 0.0 0.0 3.94698229192973E-6 0.0 2 8.55179496584775E-5 3.94698229192973E-6 0.0 2.499755451555496E-5 0.0 3 1.631419347330955E-4 3.94698229192973E-6 0.0 2.499755451555496E-5 0.0 4 4.170644621805748E-4 5.262643055906307E-6 0.0 2.762887604350811E-5 0.0 5 4.683752319756613E-4 5.262643055906307E-6 1.3156607639765767E-6 3.815416215532073E-5 0.0 6 5.31526948646537E-4 5.262643055906307E-6 1.3156607639765767E-6 4.473246597520361E-5 0.0 7 5.631028069819748E-4 6.578303819882884E-6 1.3156607639765767E-6 4.736378750315676E-5 2.6313215279531534E-6 8 6.407267920565929E-4 6.578303819882884E-6 1.3156607639765767E-6 5.3942091323039645E-5 3.94698229192973E-6 9 7.538736177585785E-4 6.578303819882884E-6 1.3156607639765767E-6 5.920473437894595E-5 5.262643055906307E-6 10-11 0.001111075515178219 7.2361342018711725E-6 1.3156607639765767E-6 6.18360559068991E-5 6.578303819882884E-6 12-13 0.0016215518916011308 8.551794965847748E-6 1.3156607639765767E-6 6.644086858081713E-5 6.578303819882884E-6 14-15 0.0022958280331391266 1.0525286111812614E-5 1.3156607639765767E-6 7.696615469262974E-5 6.578303819882884E-6 16-17 0.0031779785753854213 1.0525286111812614E-5 1.3156607639765767E-6 9.472757500631352E-5 6.578303819882884E-6 18-19 0.004130516968504463 1.249877725777748E-5 1.3156607639765767E-6 1.1972512952186849E-4 7.89396458385946E-6 20-21 0.005133050470654614 1.5130098785730632E-5 1.3156607639765767E-6 1.2432994219578651E-4 7.89396458385946E-6 22-23 0.00603362026359658 1.578792916771892E-5 1.3156607639765767E-6 1.3419739792561083E-4 7.89396458385946E-6 24-25 0.00692169127928077 1.644575954970721E-5 1.3156607639765767E-6 1.466961751833883E-4 7.89396458385946E-6 26-27 0.007853179100176187 1.71035899316955E-5 1.3156607639765767E-6 1.5590580053122434E-4 7.89396458385946E-6 28-29 0.008798481359093356 1.9734911459648653E-5 1.3156607639765767E-6 1.7037806893496668E-4 7.89396458385946E-6 30-31 0.009648398212622226 2.2366232987601806E-5 1.3156607639765767E-6 1.8221901581075587E-4 7.89396458385946E-6 32-33 0.010566071595495889 2.368189375157838E-5 1.3156607639765767E-6 1.927443019225685E-4 7.89396458385946E-6 34-35 0.011545581034276449 2.433972413356667E-5 1.3156607639765767E-6 2.0524307918034595E-4 7.89396458385946E-6 36-37 0.012617844556917359 2.6313215279531537E-5 1.3156607639765767E-6 2.2103100834806492E-4 8.551794965847748E-6 38-39 0.013801939244496278 2.894453680748469E-5 1.3156607639765767E-6 2.3945025904373697E-4 9.209625347836037E-6 40-41 0.015198513145457414 3.2233688717426135E-5 1.3156607639765767E-6 2.4668639324560814E-4 9.209625347836037E-6 42-43 0.016985180462937607 3.289151909941442E-5 1.3156607639765767E-6 2.5326469706549106E-4 9.209625347836037E-6 44-45 0.019211278475585975 3.4865010245379285E-5 1.3156607639765767E-6 2.598430008853739E-4 9.209625347836037E-6 46-47 0.021903778229064037 3.552284062736757E-5 1.3156607639765767E-6 2.690526262332099E-4 9.209625347836037E-6 48-49 0.025587628368198452 3.552284062736757E-5 1.3156607639765767E-6 2.776044211990577E-4 9.209625347836037E-6 50-51 0.030955524285222885 3.618067100935586E-5 1.3156607639765767E-6 2.9602367189472976E-4 9.209625347836037E-6 52-53 0.03881001904616305 3.683850139134415E-5 1.3156607639765767E-6 3.0391763647858925E-4 9.209625347836037E-6 54-55 0.04936227620363719 3.683850139134415E-5 1.3156607639765767E-6 3.229947175562496E-4 9.209625347836037E-6 56-57 0.06460881096697975 3.683850139134415E-5 1.3156607639765767E-6 3.4930793283578115E-4 9.209625347836037E-6 58-59 0.08708029681569968 3.683850139134415E-5 1.973491145964865E-6 3.637802012395235E-4 9.209625347836037E-6 60-61 0.11807989573651578 3.815416215532073E-5 2.6313215279531534E-6 3.716741658233829E-4 9.209625347836037E-6 62-63 0.16218018671462864 3.815416215532073E-5 2.6313215279531534E-6 3.7825246964326583E-4 9.867455729824326E-6 64-65 0.2226433506143182 3.9469822919297305E-5 2.6313215279531534E-6 3.887777557550784E-4 1.184094687578919E-5 66-67 0.30352951655321614 4.078548368327388E-5 2.6313215279531534E-6 3.9798738110291445E-4 1.184094687578919E-5 68-69 0.4078133935186735 4.144331406526216E-5 2.6313215279531534E-6 4.071970064507505E-4 1.249877725777748E-5 70-71 0.5410141784155722 4.2101144447250455E-5 2.6313215279531534E-6 4.170644621805748E-4 1.3156607639765768E-5 72-73 0.709589792103891 4.2101144447250455E-5 2.6313215279531534E-6 4.308789002023289E-4 1.3814438021754058E-5 74-75 0.9231537677860068 4.2101144447250455E-5 2.6313215279531534E-6 4.440355078420946E-4 1.578792916771892E-5 76-77 1.1820291001794758 4.2101144447250455E-5 2.6313215279531534E-6 4.5521862433589555E-4 1.578792916771892E-5 78-79 1.480046655962012 4.341680521122703E-5 2.6313215279531534E-6 4.644282496837316E-4 1.578792916771892E-5 80-81 1.8244846704799338 4.341680521122703E-5 2.6313215279531534E-6 4.815318396154271E-4 1.71035899316955E-5 82-83 2.2290543023850233 4.341680521122703E-5 2.6313215279531534E-6 4.966619384011578E-4 1.71035899316955E-5 84-85 2.7300717357453257 4.341680521122703E-5 2.6313215279531534E-6 5.052137333670055E-4 1.71035899316955E-5 86-87 3.278866731919055 4.341680521122703E-5 2.6313215279531534E-6 5.157390194788181E-4 1.71035899316955E-5 88-89 3.8477814703258963 4.341680521122703E-5 2.6313215279531534E-6 5.302112878825604E-4 1.71035899316955E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAAAC 424595 0.0 23.928585 1 GAAAAAG 496500 0.0 18.835873 9 CGGCGAG 6265 0.0 18.7874 1 CTGGAAT 207135 0.0 17.152697 1 ACGTGGA 225570 0.0 16.74508 44-45 CGGCGCT 6095 0.0 16.19667 1 TATGGCA 327030 0.0 15.649577 52-53 GACGTGG 212575 0.0 15.603174 44-45 TGGCAAG 333325 0.0 15.192838 54-55 AACTGAA 947980 0.0 15.123847 5 AAACTGA 938940 0.0 15.091469 4 GAAAACT 922005 0.0 14.947252 2 GCAAGAA 344035 0.0 14.890575 56-57 AAAACTG 940055 0.0 14.833061 3 CTTTTAT 111420 0.0 14.734097 1 ATACGAC 141005 0.0 14.67197 1 ACTGAAA 992800 0.0 14.561539 6 TACGACT 143165 0.0 14.439231 2 CGTGGAA 231805 0.0 14.436796 46-47 ACGACTC 145165 0.0 14.328301 3 >>END_MODULE