FastQCFastQC Report
Fri 27 May 2016
SRR1233103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46727462
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1656540.35451101538534235No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT1168000.24996007700996045No Hit
GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT968260.20721433575827422No Hit
GTCGTATTACAATCCTATAGTGAGTCGTATTACAATCCTATAGTGAGTCG671720.14375272511055703No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA617380.13212358933596693No Hit
GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT500520.10711474122005599No Hit
ATACGACTCACTATAGGATTGTAATACGACTCACTATAGGATTGTAATAC481190.10297798754830725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACG40300.037.653772
ACGACGG102300.028.6748831
CGACGGC96900.027.9678962
GCGCCTA54750.023.2665442
GACGGCC123900.021.9476053
CTCGTAT49150.018.896311
ACGGCCA149100.018.438414
CGGCCAG195700.016.1564771
GCGTGTT396700.015.3521241
GGGCGCT102950.014.5921
CGTGTTT392550.014.4669642
GGAAAAC955000.012.9403211
AGCGCCT75350.012.595281
CGGCGCT57350.012.5662131
AAACGAC144700.012.5563661
GTTTATT734900.012.2303751
TGACGCG15500.011.787461
TATGGCG675550.011.7350137
ATGGCGA694250.011.3551458
CTGGAAT544450.011.1859451
Produced by FastQC (version 0.11.5)