##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233103_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46727462 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.26751104093777 31.0 27.0 33.0 10.0 34.0 2 26.56982153235714 31.0 27.0 33.0 2.0 34.0 3 27.06159532054191 31.0 27.0 34.0 10.0 34.0 4 30.43077648856683 35.0 30.0 37.0 10.0 37.0 5 30.5699042032285 35.0 30.0 37.0 10.0 37.0 6 30.75838084251184 35.0 31.0 37.0 10.0 37.0 7 30.20692277273694 35.0 31.0 37.0 2.0 37.0 8 30.38962790232433 35.0 31.0 37.0 7.0 37.0 9 31.636882033096512 37.0 31.0 39.0 7.0 39.0 10-11 31.703784543230704 37.0 30.0 39.0 10.0 39.0 12-13 31.73354303942294 37.0 30.0 39.0 10.0 39.0 14-15 32.35669564077757 38.0 31.0 40.0 8.0 41.0 16-17 32.534882420962646 38.0 31.0 40.0 9.0 41.0 18-19 32.411634543729335 38.0 31.0 40.0 8.0 41.0 20-21 32.32298172967323 38.0 30.0 40.0 7.5 41.0 22-23 32.2062219214902 38.0 30.0 40.0 7.0 41.0 24-25 31.986569075803857 37.5 30.0 40.0 2.0 41.0 26-27 31.807647395871832 37.0 30.0 40.0 2.0 41.0 28-29 31.72724387427676 37.0 30.0 40.0 2.0 41.0 30-31 31.539509892491058 37.0 30.0 40.0 2.0 41.0 32-33 31.2448283302868 37.0 30.0 40.0 2.0 41.0 34-35 30.981671153036302 37.0 29.0 40.0 2.0 41.0 36-37 30.872859957598383 37.0 29.0 40.0 2.0 41.0 38-39 30.691574999301267 36.0 28.5 40.0 2.0 41.0 40-41 30.48062546174667 36.0 28.0 40.0 2.0 41.0 42-43 30.226316207800885 36.0 28.0 40.0 2.0 41.0 44-45 30.349179814217173 36.0 28.0 40.0 2.0 41.0 46-47 30.246127459265814 36.0 28.0 40.0 2.0 41.0 48-49 30.150096371594074 36.0 28.0 40.0 2.0 41.0 50-51 29.802178320748514 35.5 27.5 39.0 2.0 40.5 52-53 29.77536481651839 35.5 28.0 39.0 2.0 40.0 54-55 29.933107783598434 36.0 28.0 40.0 2.0 41.0 56-57 29.858114164214612 36.0 28.0 40.0 2.0 41.0 58-59 29.645928993532756 35.0 27.5 40.0 2.0 41.0 60-61 29.338066296003838 35.0 26.5 39.5 2.0 41.0 62-63 29.01349386362991 35.0 26.0 39.0 2.0 41.0 64-65 28.698866043698242 34.5 26.0 39.0 2.0 41.0 66-67 28.41380892889068 34.0 26.0 39.0 2.0 40.5 68-69 28.001041999670342 34.0 24.5 38.0 2.0 40.0 70-71 27.60337442465846 34.0 23.5 38.0 2.0 40.0 72-73 27.160851182116417 33.5 22.0 37.0 2.0 40.0 74-75 26.732932841505495 33.0 20.5 36.5 2.0 39.0 76-77 26.334315632207886 33.0 19.5 36.0 2.0 39.0 78-79 25.872807729210717 32.5 18.0 36.0 2.0 39.0 80-81 25.388383013397988 32.0 12.5 35.0 2.0 37.5 82-83 25.14878709868728 32.0 9.5 35.0 2.0 37.0 84-85 24.824237479450524 32.0 7.0 35.0 2.0 36.5 86-87 24.41648190736317 32.0 2.0 35.0 2.0 36.0 88-89 24.06091193225945 32.0 2.0 35.0 2.0 36.0 90-91 23.67806525635824 31.0 2.0 35.0 2.0 35.5 92-93 23.329966594376558 31.0 2.0 35.0 2.0 35.0 94-95 22.96838192281875 31.0 2.0 34.5 2.0 35.0 96-97 22.58827826985339 31.0 2.0 34.0 2.0 35.0 98-99 22.047224338869505 31.0 2.0 34.0 2.0 35.0 100-101 20.321350033947915 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.799841162067093 1101 2 5.921505652489671 1101 3 5.282612224217775 1101 4 5.559707371683604 1101 5 5.328147411558625 1101 6 5.108160500230827 1101 7 6.303610967926996 1101 8 5.959834828987752 1101 9 6.188769293641961 1101 10-11 5.60686403034186 1101 12-13 5.427045305448825 1101 14-15 6.0510506228795435 1101 16-17 5.585211303796019 1101 18-19 5.591109454035269 1101 20-21 5.5688009988417875 1101 22-23 5.389843258564685 1101 24-25 5.527608944940546 1101 26-27 5.413815912612055 1101 28-29 5.292855076785827 1101 30-31 5.314104067640567 1101 32-33 5.531331723669176 1101 34-35 5.399624075660434 1101 36-37 5.373944147572914 1101 38-39 5.419799472636644 1101 40-41 5.267250582689201 1101 42-43 5.280229643703127 1101 44-45 5.208286651605924 1101 46-47 4.978332867682635 1101 48-49 4.871661856894047 1101 50-51 4.827508231040383 1101 52-53 4.963461028587375 1101 54-55 5.225462360822821 1101 56-57 5.083949302104333 1101 58-59 5.025983847155011 1101 60-61 4.97284832844138 1101 62-63 4.89760898240781 1101 64-65 4.974962618995189 1101 66-67 4.938356719054017 1101 68-69 4.954394891856651 1101 70-71 4.860685654158566 1101 72-73 4.941218191873645 1101 74-75 4.933339369339418 1101 76-77 4.835833848424485 1101 78-79 4.941291199939148 1101 80-81 5.027355078391281 1101 82-83 4.793490401719538 1101 84-85 4.786152851514995 1101 86-87 4.648485584794727 1101 88-89 4.659020087916101 1101 90-91 4.607055333582341 1101 92-93 4.539877411167431 1101 94-95 4.4791726863334915 1101 96-97 4.341126415869159 1101 98-99 4.307887718892459 1101 100-101 3.939185105822908 1102 1 4.579209442135699 1102 2 5.778512251957135 1102 3 5.150919145836486 1102 4 5.35321101128795 1102 5 5.0727400134392155 1102 6 5.022665013097406 1102 7 6.188215429103334 1102 8 5.939565966266898 1102 9 6.030159074874696 1102 10-11 5.555255126790875 1102 12-13 5.400218953439914 1102 14-15 6.018624665336162 1102 16-17 5.635943105216402 1102 18-19 5.537681153244243 1102 20-21 5.453070342082846 1102 22-23 5.298086445490757 1102 24-25 5.452961588829432 1102 26-27 5.277575343534149 1102 28-29 5.171953647931218 1102 30-31 5.21549365351488 1102 32-33 5.447072265908517 1102 34-35 5.372831799179323 1102 36-37 5.2928480265187545 1102 38-39 5.393757431895597 1102 40-41 5.244119583515946 1102 42-43 5.306379505555707 1102 44-45 5.182884685133445 1102 46-47 4.980798961012038 1102 48-49 4.981608165404648 1102 50-51 4.848189650685015 1102 52-53 5.091135106386062 1102 54-55 5.310342022231193 1102 56-57 5.312610656813778 1102 58-59 5.159733268104198 1102 60-61 5.09235730268815 1102 62-63 5.012284192784946 1102 64-65 5.036220463587036 1102 66-67 5.011527534729801 1102 68-69 5.110098507589846 1102 70-71 4.999197260173926 1102 72-73 5.025805610824083 1102 74-75 5.090535811823884 1102 76-77 5.065012294503266 1102 78-79 4.919895868622941 1102 80-81 4.948497573041347 1102 82-83 4.866935867433849 1102 84-85 4.7996860545342095 1102 86-87 4.716025879693767 1102 88-89 4.744259059576844 1102 90-91 4.617842204895563 1102 92-93 4.598445131896501 1102 94-95 4.655989102877232 1102 96-97 4.566392639193204 1102 98-99 4.40792786006384 1102 100-101 3.9630989791375413 1103 1 4.359462238460189 1103 2 5.511709500141027 1103 3 4.968923135360093 1103 4 5.146103545539649 1103 5 4.891737407106149 1103 6 4.846463501878606 1103 7 6.005263473363609 1103 8 5.756739833659687 1103 9 5.861336150414282 1103 10-11 5.396028090953919 1103 12-13 5.291922134975817 1103 14-15 5.900926072443767 1103 16-17 5.343158004826979 1103 18-19 5.135163225483332 1103 20-21 5.084408977518926 1103 22-23 5.036928926389873 1103 24-25 5.268211894381231 1103 26-27 5.163285452642494 1103 28-29 5.076697908720384 1103 30-31 5.119521751422564 1103 32-33 5.327684565112499 1103 34-35 5.207688719790504 1103 36-37 5.1309075059933065 1103 38-39 5.127743144376428 1103 40-41 4.939390356642932 1103 42-43 4.971872300616358 1103 44-45 4.89462952990997 1103 46-47 4.773559298577336 1103 48-49 4.832389758642801 1103 50-51 4.759030029415449 1103 52-53 4.92127529239567 1103 54-55 5.066654339214569 1103 56-57 4.9935530470159115 1103 58-59 4.9600639132669215 1103 60-61 4.821412478320113 1103 62-63 4.726150742995685 1103 64-65 4.8284254716430155 1103 66-67 4.884186366369612 1103 68-69 4.896857755460736 1103 70-71 4.797577754441367 1103 72-73 4.798599722315657 1103 74-75 4.7385100072275 1103 76-77 4.771536536822161 1103 78-79 4.843902665142739 1103 80-81 4.889231228076024 1103 82-83 4.747964362219641 1103 84-85 4.74113184331684 1103 86-87 4.536164612199794 1103 88-89 4.5869755532282035 1103 90-91 4.569062300016718 1103 92-93 4.4335505205926005 1103 94-95 4.404747795129762 1103 96-97 4.4223266520140925 1103 98-99 4.214628389989706 1103 100-101 3.783386568712544 1104 1 4.319757267782045 1104 2 5.512167073501185 1104 3 4.816783912454621 1104 4 4.9844923447166956 1104 5 4.730168203831916 1104 6 4.6615335332926655 1104 7 5.955741822917961 1104 8 5.724354847132133 1104 9 5.7674419170523095 1104 10-11 5.206931595197467 1104 12-13 4.78067360858395 1104 14-15 5.575968814208025 1104 16-17 5.21195473580369 1104 18-19 5.03697043809273 1104 20-21 4.986524519122405 1104 22-23 4.988133334560974 1104 24-25 5.1348553345836265 1104 26-27 4.926705236724917 1104 28-29 4.8184121473532855 1104 30-31 4.860899661515393 1104 32-33 4.945256936427079 1104 34-35 5.0478214203843805 1104 36-37 4.900894883644554 1104 38-39 4.968790643540299 1104 40-41 4.722136936801405 1104 42-43 4.881991335118645 1104 44-45 4.69654844070395 1104 46-47 4.433714656368405 1104 48-49 4.432853773609782 1104 50-51 4.2844850427496795 1104 52-53 4.3912058441274695 1104 54-55 4.71763129949105 1104 56-57 4.736741259640649 1104 58-59 4.571999110319901 1104 60-61 4.430236060159217 1104 62-63 4.325577784598188 1104 64-65 4.383657055044026 1104 66-67 4.334153964079427 1104 68-69 4.53061845757761 1104 70-71 4.5123685596307475 1104 72-73 4.491377249047559 1104 74-75 4.5134487537802706 1104 76-77 4.513368092667605 1104 78-79 4.454189980633931 1104 80-81 4.452652963688443 1104 82-83 4.453178490662381 1104 84-85 4.439309027908802 1104 86-87 4.32875508331783 1104 88-89 4.349908110985556 1104 90-91 4.260510512160323 1104 92-93 4.196581036045018 1104 94-95 4.09641051743046 1104 96-97 4.00445642501084 1104 98-99 3.9369508511766327 1104 100-101 3.623493083682547 1105 1 4.188463689496604 1105 2 5.447753596781446 1105 3 4.747884660293696 1105 4 5.012965679894801 1105 5 3.661409748039695 1105 6 4.228052463064042 1105 7 4.74725768543686 1105 8 5.1994050545820585 1105 9 5.2723795585297495 1105 10-11 4.848499666802617 1105 12-13 4.481964491678209 1105 14-15 4.350159934364214 1105 16-17 4.479603025396958 1105 18-19 4.846803994268221 1105 20-21 4.862781109008246 1105 22-23 4.663293872073329 1105 24-25 4.921840222673076 1105 26-27 4.828895943469593 1105 28-29 4.728606632219098 1105 30-31 4.835264202843856 1105 32-33 4.96590046875847 1105 34-35 4.884069447089509 1105 36-37 4.796774880940134 1105 38-39 4.9387378730311475 1105 40-41 4.747426861472832 1105 42-43 4.93646791373347 1105 44-45 4.715567003136442 1105 46-47 4.581550468743838 1105 48-49 4.577082454788336 1105 50-51 4.38888183890689 1105 52-53 4.563547078209012 1105 54-55 4.686476290109429 1105 56-57 4.606460473037611 1105 58-59 4.578253633333333 1105 60-61 4.554042940436542 1105 62-63 4.480248653759329 1105 64-65 4.446211342070356 1105 66-67 4.504224292697764 1105 68-69 4.558648123529373 1105 70-71 4.500845163190366 1105 72-73 4.467686332678618 1105 74-75 4.494389489206537 1105 76-77 4.340603224977485 1105 78-79 4.407978345191292 1105 80-81 4.393801141579328 1105 82-83 4.2544052013317994 1105 84-85 4.242896008443562 1105 86-87 4.0425976262165335 1105 88-89 4.03630420728074 1105 90-91 3.9944318492058883 1105 92-93 3.9059173432108842 1105 94-95 3.8640425176603337 1105 96-97 3.6870684415807595 1105 98-99 3.718791546068452 1105 100-101 3.3050422382435194 1106 1 4.244192945253246 1106 2 5.335355158308165 1106 3 4.584916961580102 1106 4 4.83049959237265 1106 5 4.69972092011912 1106 6 4.511598320291252 1106 7 5.6396137790720395 1106 8 5.038250866865262 1106 9 5.2925537558581865 1106 10-11 3.7207880573993677 1106 12-13 4.690216423082756 1106 14-15 5.225649336326974 1106 16-17 4.825897691105528 1106 18-19 4.752532811144327 1106 20-21 4.849398694421488 1106 22-23 4.704524644043371 1106 24-25 4.85721595562817 1106 26-27 4.721814254364222 1106 28-29 4.706685619579204 1106 30-31 4.509568504228778 1106 32-33 4.694224186099213 1106 34-35 4.138712157231218 1106 36-37 4.398180840268338 1106 38-39 4.668323017369044 1106 40-41 4.55809824552378 1106 42-43 4.519666049232125 1106 44-45 4.440933160845319 1106 46-47 4.255666561486887 1106 48-49 4.256136761760974 1106 50-51 4.088935650243457 1106 52-53 4.253414307685475 1106 54-55 4.408626068299196 1106 56-57 4.171230186319587 1106 58-59 4.18269762364649 1106 60-61 4.052302527822 1106 62-63 4.097508226012046 1106 64-65 4.15406230539886 1106 66-67 4.221432248287179 1106 68-69 4.191039588984626 1106 70-71 4.1034470045441935 1106 72-73 4.194962812303729 1106 74-75 4.31524361802823 1106 76-77 4.273002555789443 1106 78-79 4.323703887970467 1106 80-81 4.403860309799111 1106 82-83 4.217872495032445 1106 84-85 4.20189296458652 1106 86-87 4.144152131588328 1106 88-89 4.155023647268347 1106 90-91 4.09780264551674 1106 92-93 4.033176789451893 1106 94-95 3.9315334200023635 1106 96-97 3.9015828804984487 1106 98-99 3.8291592335901043 1106 100-101 3.609996394900783 1107 1 0.9034825241487034 1107 2 1.6975525225206987 1107 3 0.9263903815098189 1107 4 1.3511540189704938 1107 5 0.9835288346897073 1107 6 1.573478247679521 1107 7 1.726402604214087 1107 8 1.542768755012176 1107 9 2.3672404796077977 1107 10-11 1.9686534967771827 1107 12-13 1.1980190676004305 1107 14-15 2.1248026989398383 1107 16-17 2.4128593253378803 1107 18-19 2.3207133202530343 1107 20-21 0.5102482574520621 1107 22-23 1.0227934389464757 1107 24-25 0.7088474068278856 1107 26-27 0.9034574614706194 1107 28-29 0.9499469307570081 1107 30-31 0.9430675633817742 1107 32-33 1.4613617642058543 1107 34-35 0.5377762695176536 1107 36-37 1.110926470657315 1107 38-39 0.6579324176608417 1107 40-41 1.5374480028352622 1107 42-43 1.6044483755111152 1107 44-45 1.0075796348418322 1107 46-47 2.006421895759882 1107 48-49 2.3000577131891298 1107 50-51 2.2926253269508834 1107 52-53 1.3604473555762588 1107 54-55 1.3583011810598542 1107 56-57 1.5467733038716602 1107 58-59 1.36443540385023 1107 60-61 1.2011237024933799 1107 62-63 1.0187833740844603 1107 64-65 0.7504839658861684 1107 66-67 0.4293686899605902 1107 68-69 1.0030806353167279 1107 70-71 1.075492632147192 1107 72-73 1.6998354711616699 1107 74-75 1.9750197260040103 1107 76-77 1.945757430513261 1107 78-79 1.8306808656195734 1107 80-81 1.3023708314909825 1107 82-83 1.5377207838814293 1107 84-85 1.4592545782075312 1107 86-87 1.5500529155156677 1107 88-89 1.2117902211323894 1107 90-91 1.5954139517531054 1107 92-93 1.565176353347983 1107 94-95 1.6024997764407551 1107 96-97 1.262724078938529 1107 98-99 1.0419329598636082 1107 100-101 1.416489366535263 1108 1 0.5157405804250921 1108 2 -0.19320658787630762 1108 3 0.2065436899905002 1108 4 -1.5951758889203482 1108 5 -0.617847377744166 1108 6 -1.9771105684519803 1108 7 -1.3171965880034229 1108 8 -1.5475017215420834 1108 9 -3.8902009892987692 1108 10-11 -1.8233781588618498 1108 12-13 -0.9335013888017052 1108 14-15 0.08017069961630341 1108 16-17 -2.040462976661029 1108 18-19 -1.6755699935939923 1108 20-21 -0.44564101656723665 1108 22-23 -0.7936293580045621 1108 24-25 -2.7570244510459787 1108 26-27 -2.1262069279987337 1108 28-29 -1.9266115134441293 1108 30-31 -1.3183781277274598 1108 32-33 -1.8607489057169317 1108 34-35 -0.7986644050640237 1108 36-37 -0.28808607466151415 1108 38-39 -2.0219545698616166 1108 40-41 -2.0622311799856483 1108 42-43 -1.576090890455113 1108 44-45 -1.1906977331477115 1108 46-47 -0.8616537242059756 1108 48-49 -1.6680025233407108 1108 50-51 -0.9114775804054887 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66-67 -0.4589275388995837 2312 68-69 -0.8804887937924342 2312 70-71 -0.2633696260249714 2312 72-73 -0.7892246393390288 2312 74-75 0.0011745336936357376 2312 76-77 0.3253227176066176 2312 78-79 0.15836217448940104 2312 80-81 0.42016737008462357 2312 82-83 0.5923474580765351 2312 84-85 -0.857564828286737 2312 86-87 -0.7502732617183767 2312 88-89 -0.3678249608199273 2312 90-91 -0.13730025965657333 2312 92-93 0.24680632617218734 2312 94-95 -0.0779609091179232 2312 96-97 -0.40631957466716173 2312 98-99 -0.6212469709246022 2312 100-101 -1.085771560460099 2313 1 -4.499292479474995 2313 2 -22.314400955833815 2313 3 -12.793829617887766 2313 4 -9.89774302300513 2313 5 -6.860575057160585 2313 6 -6.214735890625253 2313 7 -25.697730499382725 2313 8 -13.6930626833686 2313 9 -10.225660234606185 2313 10-11 -7.564550795475 2313 12-13 -6.44045325115275 2313 14-15 -6.685392498959157 2313 16-17 -7.886591775271128 2313 18-19 -9.261598767375474 2313 20-21 -7.243781707229047 2313 22-23 -8.350750602043785 2313 24-25 -11.323122385109265 2313 26-27 -10.228110975466937 2313 28-29 -8.325611634231109 2313 30-31 -7.799106643953717 2313 32-33 -7.959554085709762 2313 34-35 -7.820012847803021 2313 36-37 -9.602588635025608 2313 38-39 -9.66084061386059 2313 40-41 -8.3634806160496 2313 42-43 -8.12242148914559 2313 44-45 -8.040349931279295 2313 46-47 -8.012094617597327 2313 48-49 -8.207373810270706 2313 50-51 -8.360053254561002 2313 52-53 -8.27403760253737 2313 54-55 -8.559129071833919 2313 56-57 -8.725992874209727 2313 58-59 -9.341836449124827 2313 60-61 -9.25364472344453 2313 62-63 -10.696704084556657 2313 64-65 -10.541518957469236 2313 66-67 -10.45889138491835 2313 68-69 -10.726728246993611 2313 70-71 -10.403017492634664 2313 72-73 -10.405710494698498 2313 74-75 -10.53135639705669 2313 76-77 -10.845651734874686 2313 78-79 -11.004495628197617 2313 80-81 -11.153204345471327 2313 82-83 -11.251693338032624 2313 84-85 -11.367961095208248 2313 86-87 -11.513819130235408 2313 88-89 -11.508999143901136 2313 90-91 -11.385527798329651 2313 92-93 -11.085379029016469 2313 94-95 -10.895840983897774 2313 96-97 -10.572146094907808 2313 98-99 -10.119878079800394 2313 100-101 -8.898415477611053 2314 1 -23.38148050262996 2314 2 -22.314400955833815 2314 3 -23.07999916547848 2314 4 -25.590376664651306 2314 5 -25.57160907814779 2314 6 -25.411480423675275 2314 7 -25.697730499382725 2314 8 -25.87872596822711 2314 9 -27.16861995545574 2314 10-11 -26.65992705729905 2314 12-13 -26.876692181553622 2314 14-15 -27.596837469694982 2314 16-17 -28.31151966812899 2314 18-19 -27.50138332943833 2314 20-21 -26.887810894652564 2314 22-23 -26.723241804804022 2314 24-25 -27.13383608273294 2314 26-27 -26.933200194235013 2314 28-29 -27.426770491290597 2314 30-31 -27.213754940599976 2314 32-33 -26.87619902884019 2314 34-35 -26.906198531257708 2314 36-37 -26.791327818954286 2314 38-39 -26.094889060732847 2314 40-41 -25.835314341634145 2314 42-43 -25.935768631886514 2314 44-45 -26.097280430171413 2314 46-47 -25.510146367103378 2314 48-49 -25.988630972593704 2314 50-51 -25.494110891303777 2314 52-53 -25.190970208915566 2314 54-55 -24.9553659557727 2314 56-57 -24.796750810243203 2314 58-59 -24.63291483979241 2314 60-61 -24.348826011061423 2314 62-63 -24.05485290613861 2314 64-65 -23.729168152096612 2314 66-67 -23.414250339733005 2314 68-69 -23.005363620973775 2314 70-71 -22.76120650628527 2314 72-73 -22.359399716747266 2314 74-75 -21.9504928269639 2314 76-77 -21.607917248565148 2314 78-79 -21.210698249678586 2314 80-81 -20.715742751973018 2314 82-83 -20.51693998470514 2314 84-85 -20.223424643389876 2314 86-87 -19.830722830603342 2314 88-89 -19.47550547881747 2314 90-91 -19.10088702917672 2314 92-93 -18.791709204613774 2314 94-95 -18.441709433703576 2314 96-97 -18.065819387387524 2314 98-99 -17.564730757280362 2314 100-101 -16.014428846105627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3620061.0 3 228324.0 4 211265.0 5 223369.0 6 279473.0 7 262023.0 8 266974.0 9 293591.0 10 317611.0 11 343850.0 12 364576.0 13 385642.0 14 409307.0 15 438744.0 16 460781.0 17 470140.0 18 469229.0 19 479652.0 20 485974.0 21 476611.0 22 507818.0 23 559112.0 24 651269.0 25 779268.0 26 931178.0 27 1065445.0 28 1147555.0 29 1243664.0 30 1416084.0 31 1628092.0 32 1895906.0 33 2268934.0 34 2759949.0 35 3416888.0 36 4319397.0 37 5386375.0 38 5145116.0 39 1116663.0 40 1552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.4934710755975 21.324517230622302 15.626359252478908 25.555652441301298 2 21.54352610336667 20.103506073602144 40.26984721429479 18.08312060873639 3 23.231539844680572 25.459105099719263 28.800733459712724 22.508621595887437 4 25.31932874652853 30.050784345208765 24.717512065997184 19.912374842265525 5 22.27287117348523 33.391474257422075 28.206317558211918 16.12933701088078 6 17.404596590273268 41.02390423223339 24.909898302984388 16.661600874508952 7 14.561680753957413 19.090451833206085 47.28068646837393 19.067180944462574 8 16.628051687048732 23.983850942727962 38.70465716607648 20.683440204146827 9 18.938687063574495 25.12485150001459 33.12717906515999 22.809282371250923 10-11 19.80206203865258 34.57799794002456 25.829183269449587 19.790756751873275 12-13 18.88154899320887 27.601830588604763 34.55595180535616 18.96066861283021 14-15 19.919703923523517 27.16612171233896 32.3407084140946 20.57346595004293 16-17 22.06696902102262 26.936934523610205 31.2803835482965 19.71571290707067 18-19 21.22873878482529 28.477729955074338 30.708193970854246 19.585337289246123 20-21 21.740425935605963 29.68996191440786 29.295149340046358 19.27446280993982 22-23 21.88165474007544 29.29244704967868 30.60027173290674 18.225626477339134 24-25 21.04721121823307 29.892273097173305 30.463738459372593 18.596777225221036 26-27 21.614453223217165 28.976465785190953 29.783498106873957 19.625582884717932 28-29 21.594880415812526 29.34954571988343 29.40133347758744 19.654240386716605 30-31 20.18906116881041 29.63783550181425 30.421551623246064 19.751551706129273 32-33 20.145617528325293 29.48388148312398 29.82361333685799 20.54688765169274 34-35 20.17217968810887 29.9929152107557 28.907807861422324 20.927097239713106 36-37 20.40501095556587 29.02021658319372 29.830133083238998 20.744639378001413 38-39 20.90308248235398 28.543396059990915 29.95925861602902 20.594262841626083 40-41 21.40990465597073 28.509023395843126 29.486979286401816 20.594092661784327 42-43 21.355489134519868 29.16460297887041 29.82366247439849 19.65624541221124 44-45 21.424248061403542 29.43072639671145 29.921237292426266 19.223788249458742 46-47 21.407971561994117 28.95894128806798 29.77836951159726 19.85471763834065 48-49 21.797672136057315 29.200958616538976 29.017775037263764 19.983594210139945 50-51 20.71078721049436 29.407031789750565 29.740083142170842 20.142097857584236 52-53 20.175536538686618 29.341155469568637 30.05296007049334 20.430347921251407 54-55 19.809295302614256 29.81241835714579 29.798027874898686 20.580258465341263 56-57 20.066983379995822 29.53182316275327 29.7540333038186 20.647160153432313 58-59 20.410172384622356 29.349347658777408 29.49687438223832 20.74360557436192 60-61 20.961653734876602 29.731199097287153 29.09446445217208 20.21268271566416 62-63 20.55598696971815 29.842181456377837 29.402008822991498 20.199822750912517 64-65 21.050877641973194 28.99855190064512 29.72086437263496 20.229706084746727 66-67 21.04652888964514 29.58963806161775 29.157183924011647 20.206649124725466 68-69 21.210880231415093 29.501931861824637 29.2401800037845 20.047007902975768 70-71 21.3264867670322 29.416581623885328 29.143656464800078 20.11327514428239 72-73 20.22916686455283 30.660978880509425 28.61688110236705 20.49297315257069 74-75 20.167532006317472 29.483514364011317 29.60212152162207 20.746832108049144 76-77 20.83701642790344 29.54977885566144 28.799950419533744 20.81325429690138 78-79 20.258755946727366 30.575519480498574 28.44104502509502 20.724679547679038 80-81 20.139537164864763 30.084932579423413 29.488934256296677 20.286595999415148 82-83 20.557803407629493 30.168135555423266 29.099388950180277 20.17467208676696 84-85 20.922813063091446 30.017462155399105 29.027941086829845 20.031783694679607 86-87 21.414282335730846 30.03491073975007 28.354191239524194 20.19661568499489 88-89 21.76908787639244 29.872648360816335 28.491884301947028 19.866379460844193 90-91 21.34208971276077 30.005838676367336 28.62548771487398 20.026583895997916 92-93 21.50820861986058 30.227251013802487 28.206749119671965 20.057791246664973 94-95 21.150018540365704 30.11282598429898 28.581349591293936 20.155805884041385 96-97 21.36996952528438 30.3156357034375 27.984773491858878 20.329621279419243 98-99 21.054009474745346 30.465184406340125 28.072574043866066 20.408232075048463 100-101 22.060606113817393 30.614082727060122 27.252578931511685 20.0727322276108 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16417.0 1 12943.5 2 9341.0 3 9944.5 4 13333.5 5 17454.0 6 19142.0 7 19555.5 8 21080.5 9 23174.5 10 26418.0 11 32221.0 12 36706.5 13 43419.0 14 49556.0 15 54563.0 16 66094.5 17 82902.5 18 104634.5 19 124806.0 20 148641.0 21 174968.5 22 201329.5 23 234929.0 24 279775.5 25 340431.5 26 406699.5 27 481081.5 28 573814.5 29 675763.5 30 854021.0 31 1036004.0 32 1427302.0 33 1771447.0 34 1803745.5 35 2002406.0 36 2213025.5 37 2180808.0 38 2013898.5 39 1850859.5 40 1741663.5 41 1661586.5 42 1597092.5 43 1599942.5 44 1640714.5 45 1651376.5 46 1644657.0 47 1606336.0 48 1581247.5 49 1518787.0 50 1385721.0 51 1243459.0 52 1086717.5 53 930658.0 54 788466.5 55 669844.0 56 567595.0 57 474927.0 58 401344.0 59 338964.5 60 276393.5 61 221286.0 62 174165.5 63 132084.0 64 97289.0 65 69811.5 66 48991.0 67 35361.0 68 25893.0 69 18355.5 70 13174.0 71 9182.0 72 6448.0 73 4795.0 74 3678.0 75 2760.5 76 2080.0 77 1604.5 78 1255.5 79 1017.0 80 836.5 81 663.5 82 547.0 83 454.5 84 359.5 85 278.5 86 232.0 87 209.5 88 167.5 89 123.5 90 97.0 91 81.5 92 63.5 93 45.0 94 35.0 95 29.0 96 22.5 97 15.0 98 10.0 99 7.0 100 6.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 3.9637355009779904 2 8.934339297092574 3 4.623826990646314 4 2.9293245158489456 5 1.7782519410106203 6 2.0467706977109095 7 7.254949990650037 8 5.939066410240727 9 5.002897439625547 10-11 1.5649951200003116 12-13 2.853863537463259 14-15 3.6092940378401037 16-17 3.1741484268929474 18-19 1.750483473722583 20-21 1.5884117566667755 22-23 1.9349574346665777 24-25 2.0273880913968747 26-27 1.5274818478264451 28-29 1.2752736281718018 30-31 1.3664683949665402 32-33 1.864950422515993 34-35 1.8046047525542903 36-37 1.7377104709859912 38-39 1.1060572902504313 40-41 1.5801703503605653 42-43 2.4711753015817552 44-45 1.323406993514863 46-47 0.5722181101982385 48-49 0.7267043521430717 50-51 0.7267043521430717 52-53 0.6768396708556523 54-55 0.3168671989931745 56-57 0.19084387677635906 58-59 0.8580393259963489 60-61 0.7602392357624731 62-63 0.0 64-65 0.03104598319506418 66-67 0.21964278736131657 68-69 0.0 70-71 0.0 72-73 0.1898134334794387 74-75 0.3168671989931745 76-77 0.18859680416625238 78-79 0.2858212157981103 80-81 0.053641903341551056 82-83 0.3488526725461785 84-85 0.06338563819280406 86-87 0.03158635065606602 88-89 0.06308174837315153 90-91 0.09402607828347279 92-93 0.0628891421494281 94-95 0.06281958990197242 96-97 0.09417909322787528 98-99 0.06342522947212498 100-101 0.06362639597245834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.6727462E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.92410550130037 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50635481318909 33.3323280721196 2 9.415179914123991 7.894459920669168 3 3.615969556260544 4.54788867498472 4 1.960290068896417 3.28733630646259 5 1.1573892340147947 2.4261254176452733 6 0.789377487749292 1.9856367046051655 7 0.5657990684994335 1.6604433888215406 8 0.41537288772392383 1.393130941385406 9 0.3224848661965094 1.2167900597695676 >10 1.9586533189830395 15.40279564219866 >50 0.1722302397843128 4.9900123433967964 >100 0.10661363747002453 8.367700927856287 >500 0.007952277172168317 2.3038743884207515 >1k 0.005147402512393087 4.2210922434140565 >5k 6.80309999333406E-4 1.9840813125541301 >10k+ 5.049174245783998E-4 4.986303655696064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA 165654 0.35451101538534235 No Hit ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT 116800 0.24996007700996045 No Hit GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT 96826 0.20721433575827422 No Hit GTCGTATTACAATCCTATAGTGAGTCGTATTACAATCCTATAGTGAGTCG 67172 0.14375272511055703 No Hit ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA 61738 0.13212358933596693 No Hit GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT 50052 0.10711474122005599 No Hit ATACGACTCACTATAGGATTGTAATACGACTCACTATAGGATTGTAATAC 48119 0.10297798754830725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.009786536234302647 4.280138304965076E-6 0.0 0.0 2.140069152482538E-6 2 0.009807936925827472 1.4980484067377766E-5 0.0 8.560276609930152E-6 2.140069152482538E-6 3 0.009829337617352297 1.4980484067377766E-5 0.0 8.560276609930152E-6 2.140069152482538E-6 4 0.009942761282433871 1.4980484067377766E-5 0.0 1.070034576241269E-5 6.420207457447614E-6 5 0.00998342259633104 1.4980484067377766E-5 0.0 1.2840414914895228E-5 6.420207457447614E-6 6 0.010049764740057998 1.4980484067377766E-5 0.0 1.2840414914895228E-5 6.420207457447614E-6 7 0.010094706192260132 1.4980484067377766E-5 0.0 2.3540760677307918E-5 6.420207457447614E-6 8 0.010184589096664398 1.9260622372342843E-5 0.0 2.996096813475553E-5 6.420207457447614E-6 9 0.01031299324581335 2.140069152482538E-5 0.0 3.210103728723807E-5 8.560276609930152E-6 10-11 0.010631863549533249 2.4610795253549187E-5 0.0 3.959127932092695E-5 8.560276609930152E-6 12-13 0.011241783257990773 2.7820898982272993E-5 0.0 5.350172881206345E-5 1.3910449491136497E-5 14-15 0.011985457288478454 2.996096813475553E-5 0.0 6.527210915071742E-5 1.8190587796101574E-5 16-17 0.012949558441671838 2.996096813475553E-5 0.0 7.59724549131301E-5 2.247072610106665E-5 18-19 0.01424965045180498 3.3171071863479344E-5 0.0 8.774283525178405E-5 2.3540760677307918E-5 20-21 0.015756259135152686 3.638117559220315E-5 0.0 9.202297355674913E-5 2.3540760677307918E-5 22-23 0.017597788640863907 4.280138304965076E-5 2.140069152482538E-6 1.037933538954031E-4 2.3540760677307918E-5 24-25 0.01940828714386414 4.81515559308571E-5 2.140069152482538E-6 1.1235363050533325E-4 2.6750864406031725E-5 26-27 0.0214659636339761 5.136165965958091E-5 2.140069152482538E-6 1.209139071152634E-4 3.3171071863479344E-5 28-29 0.02399980551051542 5.992193626951106E-5 2.140069152482538E-6 1.241240108439872E-4 3.424110643972061E-5 30-31 0.02662888046434022 6.099197084575233E-5 2.140069152482538E-6 1.241240108439872E-4 3.424110643972061E-5 32-33 0.029585385998494848 6.20620054219936E-5 2.140069152482538E-6 1.2626407999646974E-4 3.638117559220315E-5 34-35 0.032974185501450946 6.420207457447614E-5 2.140069152482538E-6 1.284041491489523E-4 3.959127932092695E-5 36-37 0.03673428700236277 6.848221287944122E-5 2.140069152482538E-6 1.284041491489523E-4 4.7081521354615836E-5 38-39 0.04086676053580655 7.169231660816502E-5 3.2101037287238074E-6 1.3589439118264117E-4 5.243169423582218E-5 40-41 0.04575039834177169 7.704248948937137E-5 4.280138304965076E-6 1.3910449491136496E-4 5.350172881206345E-5 42-43 0.051067400151114564 7.704248948937137E-5 4.280138304965076E-6 1.412445640638475E-4 5.564179796454599E-5 44-45 0.05741698532653025 7.811252406561263E-5 4.280138304965076E-6 1.455247023688126E-4 5.88519016932698E-5 46-47 0.06496928936564113 8.132262779433645E-5 4.280138304965076E-6 1.487348060975364E-4 5.992193626951106E-5 48-49 0.07366332029760145 8.346269694681898E-5 4.280138304965076E-6 1.5622504813122526E-4 6.20620054219936E-5 50-51 0.08376872683562399 8.560276609930151E-5 4.280138304965076E-6 1.6371529016491416E-4 6.420207457447614E-5 52-53 0.09467558927125125 8.774283525178405E-5 4.280138304965076E-6 1.6478532474115544E-4 6.527210915071742E-5 54-55 0.10585424048924377 8.98829044042666E-5 4.280138304965076E-6 1.7120553219860304E-4 6.848221287944122E-5 56-57 0.11717306623672391 9.095293898050787E-5 4.280138304965076E-6 1.733456013510856E-4 7.383238576064757E-5 58-59 0.12829928576048064 9.202297355674913E-5 4.280138304965076E-6 1.754856705035681E-4 7.490242033688883E-5 60-61 0.1404195674055655 9.202297355674913E-5 4.280138304965076E-6 1.8083584338477447E-4 7.490242033688883E-5 62-63 0.1541352706038261 9.202297355674913E-5 4.280138304965076E-6 1.82975912537257E-4 7.490242033688883E-5 64-65 0.1700349143721951 9.202297355674913E-5 4.280138304965076E-6 1.8511598168973953E-4 7.490242033688883E-5 66-67 0.18941752068622944 9.416304270923167E-5 4.280138304965076E-6 1.8832608541846334E-4 7.490242033688883E-5 68-69 0.21267258213168094 9.523307728547294E-5 4.280138304965076E-6 1.904661545709459E-4 7.704248948937136E-5 70-71 0.24178073270917216 9.844318101419675E-5 4.280138304965076E-6 1.9260622372342843E-4 8.132262779433645E-5 72-73 0.27811054664171575 1.0058325016667929E-4 4.280138304965076E-6 1.9260622372342843E-4 8.132262779433645E-5 74-75 0.3263712460993495 1.0272331931916184E-4 4.280138304965076E-6 1.9581632745215222E-4 8.132262779433645E-5 76-77 0.38752265209696174 1.070034576241269E-4 4.280138304965076E-6 2.0330656948584113E-4 8.132262779433645E-5 78-79 0.4663510292940798 1.0807349220036817E-4 4.280138304965076E-6 2.075867077908062E-4 8.132262779433645E-5 80-81 0.5699485668620308 1.1021356135285072E-4 4.280138304965076E-6 2.1293688067201252E-4 8.132262779433645E-5 82-83 0.6992825760577367 1.1449369965781578E-4 4.280138304965076E-6 2.172170189769776E-4 8.132262779433645E-5 84-85 0.869589279212297 1.177038033865396E-4 4.280138304965076E-6 2.1935708812946016E-4 8.132262779433645E-5 86-87 1.0804995143969087 1.2305397626774593E-4 4.280138304965076E-6 2.247072610106665E-4 8.132262779433645E-5 88-89 1.3370156932554993 1.241240108439872E-4 4.280138304965076E-6 2.26847330163149E-4 8.132262779433645E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGACG 4030 0.0 37.65377 2 ACGACGG 10230 0.0 28.674883 1 CGACGGC 9690 0.0 27.967896 2 GCGCCTA 5475 0.0 23.266544 2 GACGGCC 12390 0.0 21.947605 3 CTCGTAT 4915 0.0 18.89631 1 ACGGCCA 14910 0.0 18.43841 4 CGGCCAG 19570 0.0 16.156477 1 GCGTGTT 39670 0.0 15.352124 1 GGGCGCT 10295 0.0 14.592 1 CGTGTTT 39255 0.0 14.466964 2 GGAAAAC 95500 0.0 12.940321 1 AGCGCCT 7535 0.0 12.59528 1 CGGCGCT 5735 0.0 12.566213 1 AAACGAC 14470 0.0 12.556366 1 GTTTATT 73490 0.0 12.230375 1 TGACGCG 1550 0.0 11.78746 1 TATGGCG 67555 0.0 11.735013 7 ATGGCGA 69425 0.0 11.355145 8 CTGGAAT 54445 0.0 11.185945 1 >>END_MODULE