FastQCFastQC Report
Fri 27 May 2016
SRR1233103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46727462
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1751030.37473252880714986No Hit
ATACGACTCACTATAGGGATTGTAATACGACTCACTATAGGGATTGTAAT1527540.32690412331831764No Hit
GTCGTATTACAATCCCTATAGTGAGTCGTATTACAATCCCTATAGTGAGT998320.21364738363063673No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA727350.1556579298058174No Hit
GTCGTATTACAATCCTATAGTGAGTCGTATTACAATCCTATAGTGAGTCG698980.14958655362022444No Hit
ATACGACTCACTATAGGATTGTAATACGACTCACTATAGGATTGTAATAC592710.1268440387367925No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA538850.11531762628152156No Hit
GTCGTATTACAATTCCTATAGTGAGTCGTATTACAATTCCTATAGTGAGT507920.10869839239289307No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT497900.10655404310210556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACG35250.041.160922
ACGACGG104200.027.2120361
CGACGGC107350.024.7348212
GCGCCTA54350.023.1190032
GACGGCC133350.020.3743343
GTCGAAT101350.019.307581
ACGGCCA153200.018.1057554
GGGCGCT91950.017.8205781
TATGACT183100.016.3663372
GTCTTAT185400.015.8319041
ATATGAC187750.015.5831471
CGGCCAG210450.015.504661
GCGTGTT451350.015.4899051
AAACGAC126850.015.2765081
TCGAATT129050.015.2479992
ATGACTC214650.014.9768553
TATGCCG102250.014.89155146-47
TGCCGTC103250.014.87328548-49
CGTGTTT444550.014.7297652
TGACTCA241000.014.5394134
Produced by FastQC (version 0.11.5)